Abstract：
:Minimal photosynthetic catalytic F1(αβ) core complexes, containing equimolar ratios of the α and β subunits, were isolated from membrane-bound spinach chloroplast CF1 and Rhodospirillum rubrum chromatophore RrF1. A CF1-α3β3 hexamer and RrF1-α1β1 dimer, which were purified from the respective F1(αβ) complexes, exhibit lower rates and different properties from their parent F1-ATPases. Most interesting is their complete resistance to inhibition by the general F1 inhibitor azide and the specific CF1 inhibitor tentoxin. These inhibitors were earlier reported to inhibit multisite, but not unisite, catalysis in all sensitive F1-ATPases and were therefore suggested to block catalytic site cooperativity. The absence of this typical property of all F1-ATPases in the α1β1 dimer is consistant with the view that the dimer contains only a single catalytic site. The α3β3 hexamer contains however all F1 catalytic sites. Therefore the observation that CF1-α3β3 can bind tentoxin and is stimulated by it suggests that the F1γ subunit, which is required for obtaining inhibition by tentoxin as well as azide, plays an important role in the cooperative interactions between the F1-catalytic sites.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Gromet-Elhanan Z,Sokolov Mdoi
10.1007/BF00020418subject
Has Abstractpub_date
1995-11-01 00:00:00pages
79-86issue
1-2eissn
0166-8595issn
1573-5079journal_volume
46pub_type
杂志文章abstract：:Phycobilisomes, comprised of both chromophoric (phycobiliproteins) and non-chromophoric (linker polypeptides) proteins, are light-harvesting complexes present in the prokaryotic cyanobacteria and the eukaryotic red algae. Many cyanobacteria exhibit complementary chromatic adaptation, a process which enables these orga...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00047680
更新日期：1988-07-01 00:00:00
abstract：:Due to various activities associated with nuclear industry, uranium is migrated to aquatic environments like groundwater, ponds or oceans. Uranium forms stable carbonate complexes in the oxic waters of pH 7-10 which results in a high degree of uranium mobility. Microorganisms employ various mechanisms which significan...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-013-9928-9
更新日期：2013-11-01 00:00:00
abstract：:The seasonal maximum in photosynthetic CO2 exchange rate (CER) and the cessation of leaf expansion in soybeans (Glycine max (L.) Merr) accompany fruiting under normal agricultural conditions. To investigate whether these phenomena were obligatively tied together, we caused early flowering of long-season varieties by i...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00034108
更新日期：1982-12-01 00:00:00
abstract：:In vitro studies of the carotenoid peridinin, which is the primary pigment from the peridinin chlorophyll-a protein (PCP) light harvesting complex, showed a strong dependence on the lifetime of the peridinin lowest singlet excited state on solvent polarity. This dependence was attributed to the presence of an intramol...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-005-1447-x
更新日期：2005-11-01 00:00:00
abstract：:While interest in photosynthetic thermal acclimation has been stimulated by climate warming, comparing results across studies requires consistent terminology. We identify five types of photosynthetic adjustments in warming experiments: photosynthesis as measured at the high growth temperature, the growth temperature, ...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-013-9873-7
更新日期：2014-02-01 00:00:00
abstract：:Isolated mesophyll cells from darkened leaves of the C(4) plant Digitaria sanguinalis keep functional plasmodesmata that allow the free exchange of low molecular mass compounds with the surrounding medium. This cell suspension system has been used to measure C(4) PEPC activity in situ using a spectrophotometric assay....
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/B:PRES.0000017179.31351.f0
更新日期：2004-01-01 00:00:00
abstract：:A fluorescence video imaging system utilizing relatively inexpensive commercial components is described. The instrument utilizes a black and white CCD video camera detector, a commercial video imaging board and a IBM-AT compatible computer. The color output of the imaging board greatly aids in the users ability to vis...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00048977
更新日期：1990-10-01 00:00:00
abstract：:Photoacclimation by strains of Haslea "blue" diatom species H. ostrearia and H. silbo sp. nov. ined. was investigated with rapid light curves and induction-recovery curves using fast repetition rate fluorescence. Cultures were grown to exponential phase under 50 µmol m-2 s-1 photosynthetic available radiation (PAR) an...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-018-0508-x
更新日期：2018-09-01 00:00:00
abstract：:A rapid procedure has been developed for the isolation of the photosystem two reaction centre complex (PS II RC) from a double mutant of Chlamydomonas reinhardtii, F54-14, which lacks the Photosystem one complex and the chloroplast ATPase. Thylakoid membranes are solubilised with 1.5% (w/v) Triton X-100 and the PS II ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00042974
更新日期：1995-02-01 00:00:00
abstract：:There is diverse, yet controversial fossil evidence for the existence of photosynthesis 3500 million years ago. Among the most persuasive evidence is the stromatolites described from low grade metasedimentary rocks in Western Australia and South Africa. Based on the understanding of the paleobiology of stromatolites a...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00039172
更新日期：1992-08-01 00:00:00
abstract：:The functional peculiarities and responses of the photosynthetic system in the flowering homoiochlorophyllous desiccation-tolerant (HDT) Haberlea rhodopensis and the non-desiccation-tolerant spinach were compared during desiccation and rehydration. Increasing rate of water loss clearly modifies the kinetic parameters ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-005-2440-0
更新日期：2005-08-01 00:00:00
abstract：:Four Indica and five Japonica varieties of rice (Oryza sativa L.) were examined to elucidate their differences in photosynthetic activity and dark respiratory rate as influenced by leaf nitrogen levels and temperatures. The photosynthetic rates of single leaf showed correlations with total nitrogen and soluble protein...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00032321
更新日期：1987-01-01 00:00:00
abstract：:A comparative study of photosystem II complexes isolated from tobacco (Nicotiana tabacum L. cv. John William's Broadleaf) which contains normal stacked thylakoid membranes, and from two chlorophyll deficient tobacco mutants (Su/su and Su/su var. Aurea) which have low stacked grana or essentially unstacked thylakoids w...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00032264
更新日期：1987-01-01 00:00:00
abstract：:Photosynthetic acclimation varies among species, which likely reveals variations at the biochemical level in the pathways that constitute carbon assimilation and energy transfer. Local adaptation and phenotypic plasticity affect the environmental response of photosynthesis. Phenotypic plasticity allows for a wide arra...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-013-9816-3
更新日期：2014-02-01 00:00:00
abstract：:We have used antibodies generated against synthetic peptides to determine the topology of the 43 kD chlorophyll a binding protein (CP 43) of Photosystem II. Based on the pattern of proteolytic fragments detected (on western blots) by peptide specific antibodies, a six transmembrane span topological model, with the ami...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00019041
更新日期：1994-04-01 00:00:00
abstract：:The current study confirmed earlier conclusions regarding differential ozone (O(3)) tolerances of two soybean cultivars, Essex and Forrest, and evaluated antioxidant enzyme activities of these two varieties based on their performance under environmentally relevant, elevated O(3) conditions. The experiment was conducte...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1026500911237
更新日期：2000-01-01 00:00:00
abstract：:We have constructed a mutant Synechococcus sp. PCC 7942, termed R2HECAT, in which the entire phycobilisome rod operon has been deleted. In the whole cell absorption spectra of R2HECAT, the peak corresponding to phycocyanin (PC), λmax≈620 nm, could not be detected. However, a single pigment-protein fraction with λmax=6...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00032236
更新日期：1995-07-01 00:00:00
abstract：:Historically, two main concepts guided research into possible mechanisms of light-induced atomic rearrangements in oxygenic photosynthesis: Photodecomposition of CO2 and photodecomposition of water. Both concepts envisioned photoinduced transfers of cumbersome whole atoms and not, as is currently held, photoinduced el...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00036216
更新日期：1991-09-01 00:00:00
abstract：:The nucleotide sequences of the genes coding for the subunits of the Photosystem I (PS I) core, PsaA and PsaB were determined for the marine prokaryotic oxyphototrophs Prochlorococcus sp. MED4 (CCMP1378), P. marinus SS120 (CCMP1375) and Synechococcus sp. WH7803. Divergence of these sequences from those of both freshwa...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1006445810996
更新日期：2000-01-01 00:00:00
abstract：:The water-oxidation complex of Photosystem II (PS II) contains a heteronuclear cluster of 4 Mn atoms and a Ca atom. Ligands to the metal cluster involve bridging O atoms, and O and N atoms from amino acid side-chains of the D1 polypeptide of PS II, with likely additional contributions from water and CP43. Although mod...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-005-0638-9
更新日期：2005-01-01 00:00:00
abstract：:Light-induced reaction dynamics of isolated photosynthetic membranes obtained from wild-type (WT) and reaction center (RC)-subunit deletion strains SPUHK1 (an H-subunit deletion mutant) and SK Delta LM (an (L+M) deletion mutant) of the purple non-sulphur bacterium Rhodospirillum rubrum have been investigated by femtos...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-009-9520-5
更新日期：2010-03-01 00:00:00
abstract：:Modulation of the efficiency with which leaves convert absorbed light to photochemical energy [intrinsic efficiency of open photosystem II (PSII) centers, as the ratio of variable to maximal chlorophyll fluorescence] as well as leaf xanthophyll composition (interconversions of the xanthophyll cycle pigments violaxanth...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-012-9761-6
更新日期：2012-09-01 00:00:00
abstract：:A peptide ranging from residue 229 to 240 of the D1 protein of Photosystem (PS) II was synthesized and lanthanides were used as candidates of calcium. Fluorescence and FTIR spectroscopy were used to test the conformational adaptation after lanthanide additions. Fluorescence spectroscopy showed that the synthetic pepti...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00048603
更新日期：1995-06-01 00:00:00
abstract：:The major light-harvesting system in cyanobacteria, the phycobilisome, is an essential component of the photosynthetic apparatus that regulates the utilization of the natural light source-the Sun. Earlier works revealed that the thylakoid membrane composition and its physical properties might have an important role in...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-020-00776-1
更新日期：2020-08-01 00:00:00
abstract：:Cyanobacteria perform photosynthesis with the use of large light-harvesting antennae called phycobilisomes (PBSs). These hemispherical PBSs contain hundreds of open-chain tetrapyrrole chromophores bound to different peptides, providing an arrangement in which excitation energy is funnelled towards the PBS core from wh...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-017-0417-4
更新日期：2018-03-01 00:00:00
abstract：:I provide here a personal perspective on (i) the awards that were given to young investigators at the 2008 and 2009 Gordon Research Conferences on Biochemistry/Biophysics of Photosynthesis; and (ii) the ambiance at these conferences through some photographs, particularly of the 2009 conference. ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-009-9479-2
更新日期：2009-10-01 00:00:00
abstract：:A protein was isolated from membranes of the green sulfur bacterium Chlorobium tepidum. This protein was characterized by gel electrophoresis, gel filtration, analytical ultracentrifugation and amino acid sequencing. The molecular weight of the purified protein was shown to be 26 kDa by SDS-PAGE. HPLC gelfiltration, S...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/B:PRES.0000015383.58680.56
更新日期：2004-02-01 00:00:00
abstract：:The PAM-2000 portable chlorophyll fluorometer represents one of the first commercially available instruments utilizing the Pulse Amplitude Modulation (PAM) measurement principle, and has become a widely used platform for measuring chlorophyll fluorescence in a wide range of study systems. In this paper, we describe a ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-005-9010-3
更新日期：2006-03-01 00:00:00
abstract：:Rates of net CO2 uptake were examined in developing leaves of Hydrocotyle bonariensis. Leaves that developed under high photosynthetically active radiation (48 mol m(-2) day(-1) PAR) were smaller, thicker, and reached maximum size sooner than did leaves that developed under low PAR (4.8 mol m(-2) day(-1)). Maximum net...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00028750
更新日期：1981-06-01 00:00:00
abstract：:In recent years the enzymes of the C3 photosynthetic carbon reduction (PCR) cycle have been studied using the techniques of molecular biology. In this review we discuss the primary protein sequences and structural predictions that have been made for a number of these enzymes, which, with the input of crystallographic ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00029971
更新日期：1991-01-01 00:00:00
