Abstract：
:In vitro studies of the carotenoid peridinin, which is the primary pigment from the peridinin chlorophyll-a protein (PCP) light harvesting complex, showed a strong dependence on the lifetime of the peridinin lowest singlet excited state on solvent polarity. This dependence was attributed to the presence of an intramolecular charge transfer (ICT) state in the peridinin excited state manifold. The ICT state was also suggested to be a crucial factor in efficient peridinin to Chl-a energy transfer in the PCP complex. Here we extend our studies of peridinin dynamics to reconstituted PCP complexes, in which Chl-a was replaced by different chlorophyll species (Chl-b, acetyl Chl-a, Chl-d and BChl-a). Reconstitution of PCP with different Chl species maintains the energy transfer pathways within the complex, but the efficiency depends on the chlorophyll species. In the native PCP complex, the peridinin S1/ICT state has a lifetime of 2.7 ps, whereas in reconstituted PCP complexes it is 5.9 ps (Chl-b) 2.9 ps (Chl-a), 2.2 ps (acetyl Chl-a), 1.9 ps (Chl-d), and 0.45 ps (BChl-a). Calculation of energy transfer rates using the Förster equation explains the differences in energy transfer efficiency in terms of changing spectral overlap between the peridinin emission and the absorption spectrum of the acceptor. It is proposed that the lowest excited state of peridinin is a strongly coupled S1/ICT state, which is the energy donor for the major energy transfer channel. The significant ICT character of the S1/ICT state in PCP enhances the transition dipole moment of the S1/ICT state, facilitating energy transfer to chlorophyll via the Förster mechanism. In addition to energy transfer via the S1/ICT, there is also energy transfer via the S2 and hot S1/ICT states to chlorophyll in all reconstituted PCP complexes.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Polívka T,Pascher T,Sundström V,Hiller RGdoi
10.1007/s11120-005-1447-xsubject
Has Abstractpub_date
2005-11-01 00:00:00pages
217-27issue
1-2eissn
0166-8595issn
1573-5079journal_volume
86pub_type
杂志文章abstract：:Application of target theory to the photoinactivation of Photosystem II in pea leaf discs (Park et al. 1995, 1996a,b) reveals that there is a critical light dosage below which there is complete photoprotection and above which there is photoinactivation (i.e a light-induced loss of oxygen flash yield). The critical dos...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00018219
更新日期：1996-10-01 00:00:00
abstract：:The three major chlorophyll-proteins of spinach chloroplasts were solubilized with digitonin and isolated by electrophoresis with deoxycholate. The gel bands were identified from their absorption and fluorescence spectra measured at 77 K. The slowest moving band was a Photosystem I complex (CPI); the second, a Photosy...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00054145
更新日期：1983-12-01 00:00:00
abstract：:NADP:ferredoxin oxidoreductase (EC. 1.6.7.1.) isolated from a thermophilic blue-green alga, Synechococcus sp., was stable at temperatures up to 65°C. The diaphorase and cytochrome c reductase activities of the enzyme were low at 25°C but increased with elevated temperature to reach a maximum at about 60°C. The pH-prof...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00020595
更新日期：1980-09-01 00:00:00
abstract：:The role of photosynthetic pigments in the development of separation methods in biochemistry during the period 1900-1980 is described beginning with M. Tswett who introduced separation of chlorophylls and carotenoids on columns and coined the term chromatography in 1906. In Uppsala, T. Svedberg developed the ultracent...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1024944606930
更新日期：2003-01-01 00:00:00
abstract：:Little is known about the mechanistic basis for the movement of promiscuous nucleic acids across cell membranes. To address this problem we sought conditions that would permit the entry of plasmid DNA into isolated, intact pea chloroplasts. DNA uptake did not occur normally, but was induced by hypotonic treatments, by...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00020448
更新日期：1995-11-01 00:00:00
abstract：:The post-translational modification consisting in the formation/reduction of disulfide bonds has been the subject of intense research in plants since the discovery in the 1970s that many chloroplastic enzymes are regulated by light through dithiol-disulfide exchange reactions catalyzed by oxidoreductases called thiore...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-009-9501-8
更新日期：2010-04-01 00:00:00
abstract：:Chlorosomes of green photosynthetic bacteria are the most amazing example of long-range ordered natural light-harvesting antennae. Chlorosomes are the largest among all known photosynthetic light-harvesting structures (~ 104-105 pigments in the aggregated state). The chlorosomal bacteriochlorophyll (BChl) c/d/e molecu...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-019-00707-9
更新日期：2020-12-01 00:00:00
abstract：:A recent publication (Esteban in New Phytol 217:341-342, 2018) describes how the use and citation of the assay of chlorophylls a and b extracted in aqueous 80% acetone by Arnon (Plant Physiol 2:1-15, 1949) is increasing, even in journals with high impact factors. This is a very disconcerting situation: the assay is ou...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-018-0579-8
更新日期：2019-05-01 00:00:00
abstract：:Chlorophyll (Chl) b serves an essential function in accumulation of light-harvesting complexes (LHCs) in plants. In this article, this role of Chl b is explored by considering the properties of Chls and the ligands with which they interact in the complexes. The overall properties of the Chls, not only their spectral f...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-007-9181-1
更新日期：2007-11-01 00:00:00
abstract：:We have previously investigated the response mechanisms of photosystem II complexes from spinach to strong UV and visible irradiations (Wei et al J Photochem Photobiol B 104:118-125, 2011). In this work, we extend our study to the effects of strong light on the unusual cyanobacterium Acaryochloris marina, which is abl...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-013-9946-7
更新日期：2013-11-01 00:00:00
abstract：:In appreciation of his contribution to the Photosystsem II research and commemoration of the book Photosystem II: The Light-Driven Water-Plastoquinone Oxido-Reductase, co-edited with Kimiyuki Satoh, I present here some of my recollections of Thomas John Wydrzynski and by several others with whom he has associated over...
journal_title：Photosynthesis research
pub_type： 传,历史文章,杂志文章
doi：10.1007/s11120-008-9341-y
更新日期：2008-10-01 00:00:00
abstract：:The chlorophyll-protein and polypeptide composition of manganese deficient and control sugar beet thylakoids was examined using three different detergent-electrophoresis systems. On a per chlorophyll basis, manganese deficiency reduced the amounts of CPa complex (separated by sodium dodecylsulfate (SDS)-polyacrylamide...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00014677
更新日期：1986-01-01 00:00:00
abstract：:Mike Wasielewski's pioneering work on Photosystem II photochemistry has an important place in the history of photosynthesis; we are proud to have been associated with him in making those first measurements. Here, we present our association and publications with him, and provide some of the history behind this research...
journal_title：Photosynthesis research
pub_type： 传,历史文章,杂志文章
doi：10.1007/s11120-009-9505-4
更新日期：2010-01-01 00:00:00
abstract：:The electronic structure of a Mn(II) ion bound to highly oxidizing reaction centers of Rhodobacter sphaeroides was studied in a mutant modified to possess a metal binding site at a location comparable to the Mn4Ca cluster of photosystem II. The Mn-binding site of the previously described mutant, M2, contains three car...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-013-9887-1
更新日期：2014-05-01 00:00:00
abstract：:Six chlorophyll-protein complexes are isolated from thylakoid membranes of Bryopsis corticulans by dodecyl-beta-D: -maltoside polyacrylamide gel electrophoresis. Unlike that of higher plants, the 77 K fluorescence emission spectrum of the CP1 band, the PSI core complexes of B. corticulans, presents two peaks, one at 6...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-007-9286-6
更新日期：2008-04-01 00:00:00
abstract：:Light activation of phosphoenolpyruvate carboxylase from the leaves of the C4 plant Setaria verticillata (L.) is more pronounced at low CO2 levels. The 2-fold activation observed at physiological ambient CO2 becomes 3.64-fold at 5 μL/L and completely abolished above 700 μL/L. When the stomata close under the influence...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00034834
更新日期：1988-11-01 00:00:00
abstract：:Cross-reconstitution of the extrinsic proteins and Photosystem II (PS II) from a green alga, Chlamydomonas reinhardtii, and a higher plant,Spinacia oleracea, was performed to clarify the differences of binding properties of the extrinsic proteins between these two species of organisms. (1) Chlamydomonas PsbP and PsbQ ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-004-7760-y
更新日期：2005-06-01 00:00:00
abstract：:To understand design principles for assembling photosynthetic biohybrids that incorporate precisely-controlled sites for electron injection into redox enzyme cofactor arrays, we investigated the influence of chirality in assembly of the photosensitizer ruthenium(II)bis(2,2'-bipyridine)(4-bromomethyl-4'-methyl-2,2'-bip...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-019-00697-8
更新日期：2020-02-01 00:00:00
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journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00016561
更新日期：1993-03-01 00:00:00
abstract：:A correlated influence of cation concentration and excitation energy level on PS II Abbreviations: AMPD = 2-amino-2-methyl-1,3-propanediol; DCIP = 2-6 dichlorophenol indophenol; DCIPH2 = DCIP in reduced state; DCIPr = rate of DCIP-reduction; DCMU = 3-(3,4 dichlorophenyl)1-1 dimethyl-urea; Fv = variable fluorescence; F...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00018228
更新日期：1980-06-01 00:00:00
abstract：:We investigated the dark-to-light transition in Synechococcus sp. PCC 7942 cells by a detailed analysis of fluorescence transients induced by strong red light. The transients, recorded with high data-acquisition, revealed all the steps of the fast (OJIP; 10(-5)-1 s) and slow phase (PSM(T); 1-10(3) s), kinetically dist...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-009-9405-7
更新日期：2009-03-01 00:00:00
abstract：:Photosynthesis powers our planet and is a source of inspiration for developing artificial constructs mimicking many aspects of the natural energy transducing process. In the complex machinery of photosystem II (PSII), the redox activity of the tyrosine Z (Tyrz) hydrogen-bonded to histidine 190 (His190) is essential fo...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-020-00815-x
更新日期：2021-01-11 00:00:00
abstract：:Using 77 K chlorophyll a (Chl a) fluorescence spectra in vivo, the development was studied of Photosystems II (PS II) and I (PS I) during greening of barley under intermittent light followed by continuous light at low (LI, 50 mumol m(-2) s(-1)) and high (HI, 1000 mumol m(-2) s(-1)) irradiances. The greening at HI inte...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1020943107318
更新日期：2002-01-01 00:00:00
abstract：:Recently, a number of techniques, some of them relatively new and many often used in combination, have given a clearer picture of the dynamic role of electron transport in Photosystem I of photosynthesis and of coupled cyclic photophosphorylation. For example, the photoacoustic technique has detected cyclic electron t...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00033035
更新日期：1993-06-01 00:00:00
abstract：:The D1/D2 heterodimer core is the heart of the photosystem II reaction center. A characteristic feature of this heterodimer is the differentially rapid, light-dependent degradation of the D1 protein. The D1 protein is possibly the most researched photosynthetic polypeptide, with aspects of structure-function, gene, me...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-008-9342-x
更新日期：2008-10-01 00:00:00
abstract：:Plants are exposed to a temporally and spatially heterogeneous environment, and photosynthesis is well adapted to these fluctuations. Understanding of the complex, non-linear dynamics of photosynthesis in fluctuating light requires novel-modeling approaches that involve not only the primary light and dark biochemical ...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-007-9178-9
更新日期：2007-07-01 00:00:00
abstract：:Quantum mechanics/molecular mechanics (QM/MM) hybrid methods are currently the most powerful computational tools for studies of structure/function relations and structural refinement of macrobiomolecules (e.g., proteins and nucleic acids). These methods are highly efficient, since they implement quantum chemistry tech...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-009-9467-6
更新日期：2009-11-01 00:00:00
abstract：:Several models have been proposed to interpret the mechanism of proton-pumping associated with the electron transfer reactions in the cytochrome b/f complex. Energetics considerations suggest that the proton pump is coupled to the oxidation of cytochrome b by plastoquinone. Experiments performed in living cells under ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00029737
更新日期：1986-01-01 00:00:00
abstract：:The protonophoric uncouplers carbonyl cyanide m-chlorophenylhydrazone (CCCP), 2,3,4,5,6-pentachlorophenol (PCP) and 4,5,6,7-tetrachloro-2-trifluoromethylbenzimidazole (TTFB) inhibited the Hill reaction with K3[Fe(CN)6] (but not with SiMo) in chloroplast and cyanobacterial membranes (the I50 values were approx. 1-2, 4-...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00032300
更新日期：1995-01-01 00:00:00
abstract：:Biological water oxidation, performed by a single enzyme, photosystem II, is a central research topic not only in understanding the photosynthetic apparatus but also for the development of water splitting catalysts for technological applications. Great progress has been made in this endeavor following the report of a ...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-019-00648-3
更新日期：2019-10-01 00:00:00
