Abstract:
:A recent publication (Esteban in New Phytol 217:341-342, 2018) describes how the use and citation of the assay of chlorophylls a and b extracted in aqueous 80% acetone by Arnon (Plant Physiol 2:1-15, 1949) is increasing, even in journals with high impact factors. This is a very disconcerting situation: the assay is outdated because it relies on the seriously under-estimated extinction coefficients of Mackinney (J Biol Chem 140:315-322, 1941), and the assay of chlorophylls is one of the most important, and much reported, procedures in studies of photosynthesis and related plant biological fields. Using the assay has led to the accumulation of masses of inaccurate data and confusion during the resolution of some plant biological problems. A summary not only of an accurate assay of chlorophylls in aqueous 80% acetone but also of a long-known method to correct the data obtained by Arnon's procedure (cf. Porra et al. in Biochim Biophys Acta 975:384-394, 1989) is briefly described below together with references to reliable assays in this and other solvents by other authors.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Porra RJ,Scheer Hdoi
10.1007/s11120-018-0579-8subject
Has Abstractpub_date
2019-05-01 00:00:00pages
215-219issue
2eissn
0166-8595issn
1573-5079pii
10.1007/s11120-018-0579-8journal_volume
140pub_type
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journal_title:Photosynthesis research
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更新日期:2007-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s11120-007-9135-7
更新日期:2007-07-01 00:00:00
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更新日期:2012-03-01 00:00:00
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更新日期:2006-12-01 00:00:00
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pub_type: 传,历史文章,杂志文章
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pub_type: 传,历史文章,杂志文章
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更新日期:2017-06-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章,评审
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更新日期:2007-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1991-09-01 00:00:00