Abstract：
:Heliobacteria contain Type I reaction centers (RCs) and a homodimeric core, but unlike green sulfur bacteria, they do not contain an extended antenna system. Given their simplicity, the heliobacterial RC (HbRC) should be ideal for the study of a prototypical homodimeric RC. However, there exist enormous gaps in our knowledge, particularly with regard to the nature of the secondary and tertiary electron acceptors. To paraphrase S. Neerken and J. Amesz (2001 Biochim Biophys Acta 1507:278-290): with the sole exception of primary charge separation, little progress has been made in recent years on the HbRC, either with respect to the polypeptide composition, or the nature of the electron acceptor chain, or the kinetics of forward and backward electron transfer. This situation, however, has changed. First, the low molecular mass polypeptide that contains the terminal FA and FB iron-sulfur clusters has been identified. The change in the lifetime of the flash-induced kinetics from 75 ms to 15 ms on its removal shows that the former arises from the P798+ [FA/FB]- recombination, and the latter from P798+ FX- recombination. Second, FX has been identified in HbRC cores by EPR and Mössbauer spectroscopy, and shown to be a [4Fe-4S]1+,2+ cluster with a ground spin state of S=3/2. Since all of the iron in HbRC cores is in the FX cluster, a ratio of approximately 22 Bchl g/P798 could be calculated from chemical assays of non-heme iron and Bchl g. Third, the N-terminal amino acid sequence of the FA/FB-containing polypeptide led to the identification and cloning of its gene. The expressed protein can be rebound to isolated HbRC cores, thereby regaining both the 75 ms kinetic phase resulting from P798+ [FA/FB]- recombination and the light-induced EPR resonances of FA- and FB-. The gene was named 'pshB' and the protein 'PshB' in keeping with the accepted nomenclature for Type I RCs. This article reviews the current state of knowledge on the structure and function of the HbRC.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Heinnickel M,Golbeck JHdoi
10.1007/s11120-007-9162-4subject
Has Abstractpub_date
2007-04-01 00:00:00pages
35-53issue
1eissn
0166-8595issn
1573-5079journal_volume
92pub_type
杂志文章,评审abstract：:Around 1960 experiments of Arnold and Clayton, Chance and Nishimura and Calvin and coworkers demonstrated that the primary photosynthetic electron transfer processes are not abolished by cooling to cryogenic temperatures. After a brief historical introduction, this review discusses some aspects of electron transfer in...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00041000
更新日期：1996-05-01 00:00:00
abstract：:The previous work in our lab showed that the spinach chloroplast ATP synthase epsilon mutant with 3 amino acid residues deleted from the N-terminus had much lower ability to inhibit ATP hydrolysis and block proton leakage in comparison to a mutant with 1 or 2 residues deleted from the N-terminus. The present study aim...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-004-6554-6
更新日期：2005-01-01 00:00:00
abstract：:In this communication, evidence is presented from the kinetics of QA (-) decay (where QA is the first plastoquinone electron acceptor of photosystem II) and oxygen evolution for the requirement of bicarbonate in the electron transport in a cyanobacteriumSynechocystis (Pasteur Culture Collection 6803). A large slowing ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00046879
更新日期：1988-10-01 00:00:00
abstract：:In thylakoid membranes, several peptides of high MW† are present which may interfere with the study of CP1's components. Modifying Cleveland's technique [7] for limited proteolysis, we have characterized the polypeptides found in the 60 kD region. Some may result from incomplete washing of the CF1 while others come fr...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00020071
更新日期：1980-03-01 00:00:00
abstract：:We evaluate low-temperature (low-T) emission spectra of photosystem II core complexes (PSII-cc) previously reported in the literature, which are compared with emission spectra of PSII-cc obtained in this work from spinach and for dissolved PSII crystals from Thermosynechococcus (T.) elongatus. This new spectral datase...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-015-0128-7
更新日期：2015-06-01 00:00:00
abstract：:Unique light-harvesting antennas in the green sulfur bacterium Chlorobaculum tepidum, called chlorosomes, consist of self-aggregates of bacteriochlorophyll (BChl) c. In the biosynthesis of BChl c, BciC demethoxycarbonylase removes the C132-methoxycarbonyl group to facilitate the self-aggregation of BChl c. We previous...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-018-0573-1
更新日期：2019-03-01 00:00:00
abstract：:Green sulfur bacteria and heliobacteria are strictly anaerobic phototrophs that have homodimeric type 1 reaction center complexes. Within these complexes, highly reducing substances are produced through an initial charge separation followed by electron transfer reactions driven by light energy absorption. In order to ...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-009-9521-4
更新日期：2010-06-01 00:00:00
abstract：:We have constructed a mutant Synechococcus sp. PCC 7942, termed R2HECAT, in which the entire phycobilisome rod operon has been deleted. In the whole cell absorption spectra of R2HECAT, the peak corresponding to phycocyanin (PC), λmax≈620 nm, could not be detected. However, a single pigment-protein fraction with λmax=6...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00032236
更新日期：1995-07-01 00:00:00
abstract：:The effect of light on the metabolism of ammonia was studied by subjecting detached maize leaves to 150 or 1350 μmol m(-2) s(-1) PAR during incubation with the leaf base in 2 mM (15)NH4Cl. After up to 60 min, leaves were extracted. Ammonia, glutamine, glycine, serine, alanine, and aspartate were separated by isotherma...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00049278
更新日期：1985-09-01 00:00:00
abstract：:A chlorosome is an antenna complex located on the cytoplasmic side of the inner membrane in green photosynthetic bacteria that contains tens of thousands of self-assembled bacteriochlorophylls (BChls). Green bacteria are known to incorporate various esterifying alcohols at the C-17 propionate position of BChls in the ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-014-0017-5
更新日期：2014-10-01 00:00:00
abstract：:Three Chl-protein complexes were isolated from thylakoid membranes of Bryopsis maxima and Ulva pertusa, marine green algae that inhabit the intertidal zone of the Pacific Ocean off the eastern coast of Japan by dodecyl-beta-D-maltoside polyacrylamide gel electrophoresis. The slowest-moving fractions showed low Chl a/b...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-006-9064-x
更新日期：2006-07-01 00:00:00
abstract：:Modulation of the efficiency with which leaves convert absorbed light to photochemical energy [intrinsic efficiency of open photosystem II (PSII) centers, as the ratio of variable to maximal chlorophyll fluorescence] as well as leaf xanthophyll composition (interconversions of the xanthophyll cycle pigments violaxanth...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-012-9761-6
更新日期：2012-09-01 00:00:00
abstract：:When 23°C-grown potato leaves (Solanum tuberosum L.) were exposed for 15 min to elevated temperatures in weak light, a dramatic and preferential inactivation of Photosystem (PS) II was observed at temperatures higher than about 38°C. In vivo photoacoustic measurements indicated that, concomitantly with the loss of PS ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00017756
更新日期：1996-01-01 00:00:00
abstract：:A rapid procedure has been developed for the isolation of the photosystem two reaction centre complex (PS II RC) from a double mutant of Chlamydomonas reinhardtii, F54-14, which lacks the Photosystem one complex and the chloroplast ATPase. Thylakoid membranes are solubilised with 1.5% (w/v) Triton X-100 and the PS II ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00042974
更新日期：1995-02-01 00:00:00
abstract：:Although it has been believed that wild-type plants are capable of protecting photosystem I (PSI) under high light, our previous study indicates that PSI is sensitive to high light in the shade-established tree species Psychotria rubra. However, the underlying physiological mechanisms are unclear. In this study, we ex...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-016-0275-5
更新日期：2016-07-01 00:00:00
abstract：:The quantum yield for light-induced H2 generation was measured for a previously optimized bio-hybrid cytochrome c 6-crosslinked PSI(C13G)-1,8-octanedithiol-[FeFe]-H2ase(C97G) (PSI-H2ase) nanoconstruct. The theoretical quantum yield for the PSI-H2ase nanoconstruct is 0.50 molecules of H2 per photon absorbed, which equa...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-014-0064-y
更新日期：2016-01-01 00:00:00
abstract：:In this report we demonstrate sulfite interaction with oxygen and PSII electron acceptors (ferricyanide and para-benzoquinone) during measurement of oxygen evolution in chloroplasts. Redox potentials of oxygen, ferricyanide and para-benzoquinone allow them to compete for sulfite. Without taking this into account, sulf...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00019595
更新日期：1987-01-01 00:00:00
abstract：:With the discovery of targeted gene replacement, moss biology has been rapidly advancing over the last 10 years. This study demonstrates the usefulness of moss as a model organism for plant photosynthesis research. The two mosses examined in this study, Physcomitrella patens and Ceratodon purpureus, are easily culture...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-004-5577-3
更新日期：2005-01-01 00:00:00
abstract：:The factors governing quinone protein binding in the photosynthetic bacterium Rps. viridis are investigated via use of empirical intermolecular energy calculations using the GRID method (PJ Goodford (1985) J Med Chem 28: 849-854). A probe atom corresponding to a carbonyl oxygen atom is used to investigate regions exhi...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00027142
更新日期：1994-01-01 00:00:00
abstract：:Vyacheslav Vasilevich (V.V.) Klimov (or Slava, as most of us called him) was born on January 12, 1945 and passed away on May 9, 2017. He began his scientific career at the Bach Institute of Biochemistry of the USSR Academy of Sciences (Akademy Nauk (AN) SSSR), Moscow, Russia, and then, he was associated with the Insti...
journal_title：Photosynthesis research
pub_type： 传,历史文章,杂志文章
doi：10.1007/s11120-017-0440-5
更新日期：2018-04-01 00:00:00
abstract：:The post-translational modification consisting in the formation/reduction of disulfide bonds has been the subject of intense research in plants since the discovery in the 1970s that many chloroplastic enzymes are regulated by light through dithiol-disulfide exchange reactions catalyzed by oxidoreductases called thiore...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-009-9501-8
更新日期：2010-04-01 00:00:00
abstract：:The kinetic and spectroscopic properties of the secondary electron acceptor A1 were determined by flash absorption spectroscopy at room and cryogenic temperatures in a Photosystem I (PS I) core devoid of the iron-sulfur clusters FX, FB and FA. It was shown earlier (Warren, P.V., Golbeck, J.H. and Warden, J.T. (1993) B...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00015559
更新日期：1995-09-01 00:00:00
abstract：:C3 carbon fixation has a bad reputation, primarily because it is associated with photorespiration, a biochemical pathway thought to waste a substantial amount of the carbohydrate produced in a plant. This review presents evidence collected over nearly a century that (1) Rubisco when associated with Mn(2+) generates ad...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-014-0056-y
更新日期：2015-02-01 00:00:00
abstract：:A short list of the twentieth century's dominant figures in photosynthesis would unquestionably include Otto Warburg. One of his many discoveries, the 'bicarbonate effect' remains a lasting puzzle to his heirs in the field. Recent developments in this area of research have renewed interest and call for a re-examinatio...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1020447030191
更新日期：2002-01-01 00:00:00
abstract：:The linear, four-step oxidation of water to molecular oxygen by photosystem II requires cooperation between redox reactions driven by light and a set of redox reactions involving the S-states within the oxygen-evolving complex. The oxygenevolving complex is a highly ordered structure in which a number of polypeptides ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00037001
更新日期：1985-01-01 00:00:00
abstract：:Artificial aggregates of bacteriochlorophyllc (BChlc) were formed in an aqueous medium in the presence of a lipid, monogalactosyl diglyceride (MGDG), and the optical properties of those aggregates were studied by absorption and circular dichroism (CD) mainly. Four BChlc homologs, ([E,E]BChlc F, [P,E]BChlc F, [E,M]BChl...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF02184164
更新日期：1994-07-01 00:00:00
abstract：:Four Indica and five Japonica varieties of rice (Oryza sativa L.) were examined to elucidate their differences in photosynthetic activity and dark respiratory rate as influenced by leaf nitrogen levels and temperatures. The photosynthetic rates of single leaf showed correlations with total nitrogen and soluble protein...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00032321
更新日期：1987-01-01 00:00:00
abstract：:The oxidation kinetics under far-red light (FRL) of photosystem I (PSI) high potential donors P700, plastocyanin (PC), and cytochrome f (Cyt f) were investigated in sunflower leaves with the help of a new high-sensitivity photometer at 810 nm. The slopes of the 810 nm signal were measured immediately before and after ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-007-9224-7
更新日期：2007-10-01 00:00:00
abstract：:A model is presented describing the relationship between chlorophyll fluorescence quenching and photoinhibition of Photosystem (PS) II-dependent electron transport in chloroplasts. The model is based on the hypothesis that excess light creates a population of inhibited PS II units in the thylakoids. Those units are su...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00042009
更新日期：1991-12-01 00:00:00
abstract：:Dan Arnon, Bob Whatley, Mary Belle Allen, and their colleagues, were the first to obtain evidence for 'complete photosynthesis by isolated chloroplasts' albeit at rates which were 1% or less of those displayed by the intact leaf. By the 1960s, partly in the hope of confirming full functionality, there was a perceived ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1024962328483
更新日期：2003-01-01 00:00:00
