Artificial inhibitory effects of sulfite on photosystem II activity measured by oxygen evolution in chloroplasts.

Abstract:

:In this report we demonstrate sulfite interaction with oxygen and PSII electron acceptors (ferricyanide and para-benzoquinone) during measurement of oxygen evolution in chloroplasts. Redox potentials of oxygen, ferricyanide and para-benzoquinone allow them to compete for sulfite. Without taking this into account, sulfite inhibition of oxygen evolution can be overestimated, since sulfite consumes oxygen and reduces ferricyanide or para-benzoquinone during the measurement. In order to correctly measure the rate of oxygen evolution in chloroplasts, it is necessary to avoid presence of sulfite during the measurement. After overcoming the artifact, mentioned above, we confirm the sulfite inhibition of oxygen evolution in chloroplasts but at a lesser extent than earlier reported. This, however, is a pretreatment effect.

journal_name

Photosynth Res

journal_title

Photosynthesis research

authors

Beauregard M,Popovic R

doi

10.1007/BF00019595

subject

Has Abstract

pub_date

1987-01-01 00:00:00

pages

89-94

issue

1

eissn

0166-8595

issn

1573-5079

journal_volume

14

pub_type

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