Abstract:
:Characteristics of primary phases in chlorophyll a fluorescence transients based on room temperature in vivo measurement with a Plant Productivity Fluorometer (Brancker Model SF-10) can be greatly facilitated by coupling the instrument to a fast data acquisition system. The SF-10 was linked to a Multitech Industrial Corporation Microprofessor Microcomputer and further modified to ensure simultaneous onset of light activation and signal capture. Circuit diagrams and program listings are given in detail.This microprocessor system is capable of capturing signal changes over a minimum period of 200 milliseconds to a maximum of 6 seconds. Accuracy of recorded data is dependent on rate of change of the input signal and the recording time period. Acquisition and storage of 5000 points from zero to 300 milliseconds ensured clear resolution of Fo, I and D when played back over 120 seconds on a chart recorder. For routine use, the primary transient can be captured over 0-2 seconds and then replayed as an accompaniment to standard slower presentation of primary plus secondary transients. Coincidence of signal amplitude for Fp on both systems can then be ascertained while retaining adequate resolution of Fo and I.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Norrish R,Kriedemann PE,Wiskich JTdoi
10.1007/BF00041817subject
Has Abstractpub_date
1983-01-01 00:00:00pages
213-27issue
1eissn
0166-8595issn
1573-5079journal_volume
4pub_type
杂志文章abstract::For maximal rates of CO2 assimilation in isolated intact spinach chloroplasts the generation of the adequate NADPH/ATP ratio is achieved either by cyclic electron flow around photosystem I or by linear electron transport to oxaloacetate, nitrite or oxygen (Mehler-reaction). The interrelationships between these poising...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00019060
更新日期:1994-10-01 00:00:00
abstract::Oxygen may enhance CO2-saturated photosynthesis in intact leaves, which display the Warburg effect when illuminated at the current atmospheric level of CO2 and O2, of about 350 microl l(-1) and 21%, respectively. The magnitude of the stimulation depends on irradiance. The K(M)(O2) of the stimulation is 128 microM (10....
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-006-9050-3
更新日期:2006-06-01 00:00:00
abstract::We present the wavelength dependence of homogeneous holewidths of persistent spectral holes burnt in O2-evolving Photosystem II core complexes isolated from spinach, in the temperature range 2.5-8 K. The data supports the assignment that those chlorophylls which undergo persistent spectral hole-burning are specific CP...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-004-7927-6
更新日期:2005-06-01 00:00:00
abstract::Recommendations are given for naming of genes coding for reaction center, antenna and electron transport proteins in green photosynthetic bacteria and heliobacteria. ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF02184142
更新日期:1994-07-01 00:00:00
abstract::The photosynthetic sulfur bacterium, Chromatium vinosum, was cultured in inorganic photographic processing solutions containing silver thiosulfate complex salt (AgNa3(S2O3)2) under light. It was found that Chromatium was resistant to Ag and accumulated granular silver in the membrane during growth. The amount of Ag ac...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00118318
更新日期:1986-01-01 00:00:00
abstract::Recent magnetic-resonance work on YŻ suggests that this species exhibits considerable motional flexibility in its functional site and that its phenol oxygen is not involved in a well-ordered hydrogen-bond interaction (Tang et al., submitted; Tommos et al., in press). Both of these observations are inconsistent with a ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00020428
更新日期:1995-11-01 00:00:00
abstract::The linear dichroism of Photosystem I particles containing 10 chlorophylls per P700 has been investigated at 10 K. The particles were oriented by uniaxial squeezing of polyacrylamide gels. The oxidation state of P700 was altered either by incubation of the gels with redox mediators or by low temperature illumination. ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00047172
更新日期:1989-07-01 00:00:00
abstract::Six chlorophyll-protein complexes are isolated from thylakoid membranes of Bryopsis corticulans by dodecyl-beta-D: -maltoside polyacrylamide gel electrophoresis. Unlike that of higher plants, the 77 K fluorescence emission spectrum of the CP1 band, the PSI core complexes of B. corticulans, presents two peaks, one at 6...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-007-9286-6
更新日期:2008-04-01 00:00:00
abstract::The pigment composition of the light-harvesting complexes of Photosystem II (LHC II) has been determined for lettuce (Lactuca sativa). In common with other members of the composite, the photosynthetic tissues of this species may contain large amounts of the carotenoid lactucaxanthin (ε, ε-carotene-3,3'-diol) in additi...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00029940
更新日期:1995-03-01 00:00:00
abstract::Using a novel, pulsed micro-second time-resolved photoacoustic (PA) instrument, we measured thermal dissipation and energy storage (ES) in the intact cells of wild type (WT) Chlamydomonas reinhardtii, and mutants lacking either PSI or PSII reaction centers (RCs). On this time scale, the kinetic contributions of the th...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-011-9682-9
更新日期:2011-09-01 00:00:00
abstract::Prior to the 1960s, the model for the molecular structure of cell membranes consisted of a lipid bilayer held in place by a thin film of electrostatically-associated protein stretched over the bilayer surface: (the Danielli-Davson-Robertson "unit membrane" model). Andrew Benson, an expert in the lipids of chloroplast ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-007-9159-z
更新日期:2007-05-01 00:00:00
abstract::When CO(2) supply is limited, aquatic photosynthetic organisms induce a CO(2)-concentrating mechanism (CCM) and acclimate to the CO(2)-limiting environment. Although the CCM is well studied in unicellular green algae such as Chlamydomonas reinhardtii, physiological aspects of the CCM and its associated genes in multic...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-010-9614-0
更新日期:2011-09-01 00:00:00
abstract::The electron transport rates of photosystems II and I, amounts of electron carriers, coupling factor activity and photosynthetic rates were investigated in thylakoids isolated from pea plants grown under a wide range of light intensities (16 h light-8 h dark). The electron transport rates of PS II and PS I, as partial...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00028525
更新日期:1984-06-01 00:00:00
abstract::Photosynthesis was well-established on the earth at least 3.5 thousand million years ago, and it is widely believed that these ancient organisms had similar metabolic capabilities to modern cyanobacteria. This requires that development of two photosystems and the oxygen evolution capability occurred very early in the...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:
更新日期:1992-01-01 00:00:00
abstract::Energy transfer of the light harvesting complex LHC-II trimer, extracted from spinach, was studied in the Q(y) region at room temperature by femtosecond transient absorption spectroscopy. Configuration interaction exciton method [Linnanto et al. (1999) J Phys Chem B 103: 8739-8750] and 2.72 A structural information re...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-005-9004-1
更新日期:2006-03-01 00:00:00
abstract::To investigate the impact of iron deficiency on bioenergetic pathways in Chlamydomonas, we compared growth rates, iron content, and photosynthetic parameters systematically in acetate versus CO(2)-grown cells. Acetate-grown cells have, predictably (2-fold) greater abundance of respiration components but also, counter-...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-010-9562-8
更新日期:2010-07-01 00:00:00
abstract::The protonophoric uncouplers carbonyl cyanide m-chlorophenylhydrazone (CCCP), 2,3,4,5,6-pentachlorophenol (PCP) and 4,5,6,7-tetrachloro-2-trifluoromethylbenzimidazole (TTFB) inhibited the Hill reaction with K3[Fe(CN)6] (but not with SiMo) in chloroplast and cyanobacterial membranes (the I50 values were approx. 1-2, 4-...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00032300
更新日期:1995-01-01 00:00:00
abstract::Here, we describe a new imaging setup able to assess in vivo photosynthetic activity. The system specifically measures time-resolved chlorophyll fluorescence in response to light. It is composed of a fast digital camera equipped with a wide-angle lens for the analysis of samples up to 10 x 10 cm, i.e. entire plants or...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-009-9487-2
更新日期:2009-10-01 00:00:00
abstract::A three-dimensional model of the core proteins D1 and D2, including the cofactors, that form the Photosystem II reaction centre of pea (Pisum sativum), has been generated. This model was built with a rule-based computer modelling system using the information from the crystal structures of the photosynthetic reaction c...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00033446
更新日期:1992-11-01 00:00:00
abstract::The post-translational modification consisting in the formation/reduction of disulfide bonds has been the subject of intense research in plants since the discovery in the 1970s that many chloroplastic enzymes are regulated by light through dithiol-disulfide exchange reactions catalyzed by oxidoreductases called thiore...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-009-9501-8
更新日期:2010-04-01 00:00:00
abstract::In this paper we compared the pigment composition, photochemical activity, chloroplast ultrastructure, thylakoid membrane polypeptide composition and ribosomal content of wild-type and seven light-sensitive mutants of Chlamydomonas reinhardii.All the mutants had low chlorophyll and carotenoid content compared to wild-...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00032261
更新日期:1982-01-01 00:00:00
abstract::Dan Arnon, Bob Whatley, Mary Belle Allen, and their colleagues, were the first to obtain evidence for 'complete photosynthesis by isolated chloroplasts' albeit at rates which were 1% or less of those displayed by the intact leaf. By the 1960s, partly in the hope of confirming full functionality, there was a perceived ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1024962328483
更新日期:2003-01-01 00:00:00
abstract::Light activation of phosphoenolpyruvate carboxylase from the leaves of the C4 plant Setaria verticillata (L.) is more pronounced at low CO2 levels. The 2-fold activation observed at physiological ambient CO2 becomes 3.64-fold at 5 μL/L and completely abolished above 700 μL/L. When the stomata close under the influence...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00034834
更新日期:1988-11-01 00:00:00
abstract::Photoinhibition of the light-induced Photosystem I (PS I) electron transfer activity from the reduced dichlorophenol indophenol to methyl viologen was studied. PS I preparations with Chl/P700 ratios of about 180 (PS I-180), 100 (PS I-100) and 40 (PS I(HA)-40) were isolated from spinach thylakoid membranes by the treat...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00016175
更新日期:1996-02-01 00:00:00
abstract::Minimal photosynthetic catalytic F1(αβ) core complexes, containing equimolar ratios of the α and β subunits, were isolated from membrane-bound spinach chloroplast CF1 and Rhodospirillum rubrum chromatophore RrF1. A CF1-α3β3 hexamer and RrF1-α1β1 dimer, which were purified from the respective F1(αβ) complexes, exhibit ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00020418
更新日期:1995-11-01 00:00:00
abstract::Using 77 K chlorophyll a (Chl a) fluorescence spectra in vivo, the development was studied of Photosystems II (PS II) and I (PS I) during greening of barley under intermittent light followed by continuous light at low (LI, 50 mumol m(-2) s(-1)) and high (HI, 1000 mumol m(-2) s(-1)) irradiances. The greening at HI inte...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1020943107318
更新日期:2002-01-01 00:00:00
abstract::The three major chlorophyll-proteins of spinach chloroplasts were solubilized with digitonin and isolated by electrophoresis with deoxycholate. The gel bands were identified from their absorption and fluorescence spectra measured at 77 K. The slowest moving band was a Photosystem I complex (CPI); the second, a Photosy...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00054145
更新日期:1983-12-01 00:00:00
abstract::Effect of water-soluble and stable sucrose-bound iron oxyhydroxide nanoparticles [Fe[III] sucrose complex (FSC)] on the efficiency of electron transport in the photosystem II membranes was studied. FSC significantly increases (by a factor 1.5) the rate of light-induced oxygen evolution in the presence of alternative e...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-019-00647-4
更新日期:2019-10-01 00:00:00
abstract::Photosynthesis powers our planet and is a source of inspiration for developing artificial constructs mimicking many aspects of the natural energy transducing process. In the complex machinery of photosystem II (PSII), the redox activity of the tyrosine Z (Tyrz) hydrogen-bonded to histidine 190 (His190) is essential fo...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-020-00815-x
更新日期:2021-01-11 00:00:00
abstract::Inorganic phosphate participates in many fundamental processes within the plant cell. Its broad influence on plant metabolism is related to such key operations as metabolite transport, enzyme regulation and carbohydrate metabolism in general. This review discusses these topics with special emphasis on the role assigne...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00016551
更新日期:1993-03-01 00:00:00