A new evaluation of chlorophyll absorption in photosynthetic membranes.

Abstract:

:The three major chlorophyll-proteins of spinach chloroplasts were solubilized with digitonin and isolated by electrophoresis with deoxycholate. The gel bands were identified from their absorption and fluorescence spectra measured at 77 K. The slowest moving band was a Photosystem I complex (CPI); the second, a Photosystem II complex (Cpa); and the third, a chlorophyll a-b, antenna complex (LHCP). When absorption spectra (630-730 nm) of the bands were added in the proportions found in the gel, the sum closely matched the absorption of the chloroplasts both before and after solubilization. Thus these spectra represent the native absorption of the major antenna chlorophyll-proteins of green plants. Each of these spectra was resolved with a computer assisted, curve-fitting program into 8 mixed Gaussian-Lorentzian shaped components. The major, Chl a components in the 3 fractions were different both in peak positions and bandwidths. This result suggests that each chlorophyll-protein has its own unique set of chlorophyll a spectral forms or components.

journal_name

Photosynth Res

journal_title

Photosynthesis research

authors

Brown JS

doi

10.1007/BF00054145

subject

Has Abstract

pub_date

1983-12-01 00:00:00

pages

375-83

issue

4

eissn

0166-8595

issn

1573-5079

journal_volume

4

pub_type

杂志文章
  • Light effects on leaf development and photosynthetic capacity of Hydrocotyle bonariensis Lam.

    abstract::Rates of net CO2 uptake were examined in developing leaves of Hydrocotyle bonariensis. Leaves that developed under high photosynthetically active radiation (48 mol m(-2) day(-1) PAR) were smaller, thicker, and reached maximum size sooner than did leaves that developed under low PAR (4.8 mol m(-2) day(-1)). Maximum net...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/BF00028750

    authors: Longstreth DJ,Hartsock TL,Nobel PS

    更新日期:1981-06-01 00:00:00

  • Supramolecular organization of the photosynthetic chain in chromatophores and cells of Rhodobacter sphaeroides.

    abstract::Flash-induced kinetics of the membrane potential increase related to electron transfer within the cytochrome (cyt) b/c1 complex (Phase III) and that of cyt c1+c2 reduction have been measured as a function of myxothiazol concentration in isolated chromatophores and whole cells of Rhodobacter sphaeroides. Upon addition ...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/BF00041020

    authors: Joliot P,Verméglio A,Joliot A

    更新日期:1996-05-01 00:00:00

  • Isolation and characterisation of the Photosystem two reaction centre complex from a double mutant of Chlamydomonas reinhardtii.

    abstract::A rapid procedure has been developed for the isolation of the photosystem two reaction centre complex (PS II RC) from a double mutant of Chlamydomonas reinhardtii, F54-14, which lacks the Photosystem one complex and the chloroplast ATPase. Thylakoid membranes are solubilised with 1.5% (w/v) Triton X-100 and the PS II ...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/BF00042974

    authors: Alizadeh S,Nixon PJ,Telfer A,Barber J

    更新日期:1995-02-01 00:00:00

  • Re-examination of Mg-dechelation reaction in the degradation of chlorophylls using chlorophyllin a as a substrate.

    abstract::The Mg-dechelating activity of extracts of Chenopodium album (goosefoot) was investigated using an artificial substrate, chlorophyllin a. The activity was measured spectrophotometrically by the formation of a reaction product, pheophorbin a (Mg-free chlorin), after release of the central Mg. The Mg-releasing protein w...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1023/A:1020915812770

    authors: Suzuki T,Shioi Y

    更新日期:2002-01-01 00:00:00

  • Regulation and function of xanthophyll cycle-dependent photoprotection in algae.

    abstract::The xanthophyll cycle represents one of the important photoprotection mechanisms in plant cells. In the present review, we summarize current knowledge about the violaxanthin cycle of vascular plants, green and brown algae, and the diadinoxanthin cycle of the algal classes Bacillariophyceae, Xanthophyceae, Haptophyceae...

    journal_title:Photosynthesis research

    pub_type: 杂志文章,评审

    doi:10.1007/s11120-010-9536-x

    authors: Goss R,Jakob T

    更新日期:2010-11-01 00:00:00

  • Changes in the binding and inhibitory properties of urea/triazine-type herbicides upon phospholipid and galactolipid depletion in the outer monolayer of thylakoid membranes : Different behaviour of atrazine-susceptible and-resistant biotypes of Solanum ni

    abstract::The binding characteristics and the inhibitory power of atrazine and DCMU towards uncoupled electron flow activity were studied in acyl lipid-depleted thylakoid membranes from atrazine-susceptible and-resistant biotypes of Solanum nigrum L. For this purpose, phospholipase A2 from Vipera russelli and the lipase from Rh...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/BF00049537

    authors: Siegenthaler PA,Mayor JP

    更新日期:1992-01-01 00:00:00

  • Absorbance changes accompanying the fast fluorescence induction in the purple bacterium Rhodobacter sphaeroides.

    abstract::The authors present a study of the fluorescence and absorbance transients occurring in whole cells of purple nonsulfur bacterium Rhodobacter sphaeroides on the millisecond timescale under pulsed actinic illumination. The fluorescence induction curve is interpreted in terms of combination of effects of redox changes in...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/s11120-010-9569-1

    authors: Bína D,Litvín R,Vácha F

    更新日期:2010-08-01 00:00:00

  • International conference on "Photosynthesis research for sustainability-2013: in honor of Jalal A. Aliyev", held during June 5-9, 2013, Baku, Azerbaijan.

    abstract::In this brief report, we provide a pictorial essay on an international conference "Photosynthesis Research for Sustainability-2013 in honor of Jalal A. Aliyev" that was held in Baku, Azerbaijan, during June 5-9, 2013 ( http://photosynthesis2013.cellreg.org/ ). We begin this report with a brief note on Jalal Aliyev, th...

    journal_title:Photosynthesis research

    pub_type:

    doi:10.1007/s11120-013-9901-7

    authors: Allakhverdiev SI,Huseynova IM,Govindjee

    更新日期:2013-12-01 00:00:00

  • The chloroplastic thiol reducing systems: dual functions in the regulation of carbohydrate metabolism and regeneration of antioxidant enzymes, emphasis on the poplar redoxin equipment.

    abstract::The post-translational modification consisting in the formation/reduction of disulfide bonds has been the subject of intense research in plants since the discovery in the 1970s that many chloroplastic enzymes are regulated by light through dithiol-disulfide exchange reactions catalyzed by oxidoreductases called thiore...

    journal_title:Photosynthesis research

    pub_type: 杂志文章,评审

    doi:10.1007/s11120-009-9501-8

    authors: Chibani K,Couturier J,Selles B,Jacquot JP,Rouhier N

    更新日期:2010-04-01 00:00:00

  • Accumulation of silver by Chromatium vinosum from solutions containing silver thiosulfate.

    abstract::The photosynthetic sulfur bacterium, Chromatium vinosum, was cultured in inorganic photographic processing solutions containing silver thiosulfate complex salt (AgNa3(S2O3)2) under light. It was found that Chromatium was resistant to Ag and accumulated granular silver in the membrane during growth. The amount of Ag ac...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/BF00118318

    authors: Kitajima M

    更新日期:1986-01-01 00:00:00

  • A new setup for in vivo fluorescence imaging of photosynthetic activity.

    abstract::Here, we describe a new imaging setup able to assess in vivo photosynthetic activity. The system specifically measures time-resolved chlorophyll fluorescence in response to light. It is composed of a fast digital camera equipped with a wide-angle lens for the analysis of samples up to 10 x 10 cm, i.e. entire plants or...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/s11120-009-9487-2

    authors: Johnson X,Vandystadt G,Bujaldon S,Wollman FA,Dubois R,Roussel P,Alric J,Béal D

    更新日期:2009-10-01 00:00:00

  • Subunit organization of PSI particles from brown algae and diatoms: polypeptide and pigment analysis.

    abstract::P700 enriched fractions were isolated from two brown algae and one diatom using sucrose density centrifugation after digitinin solubilization. They had a Chl a/P700 ratio of about 250 to 375 according to the species, they were enriched in long-wavelength absorbing Chl a and exhibited a fluorescence emission maximum at...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/BF00035009

    authors: Berkaloff C,Caron L,Rousseau B

    更新日期:1990-02-01 00:00:00

  • Flash-induced proton transfer in photosynthetic bacteria.

    abstract::A proton electrochemical potential across the membranes of photosynthetic purple bacteria is established by a light-driven proton pump mechanism: the absorbed light in the reaction center initiates electron transfer which is coupled to the vectorial displacement of protons from the cytoplasm to the periplasm. The stoi...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/BF02185435

    authors: Maróti P

    更新日期:1993-07-01 00:00:00

  • The role of charge-transfer states in the spectral tuning of antenna complexes of purple bacteria.

    abstract::The LH2 antenna complexes of purple bacteria occur, depending on light conditions, in various different spectroscopic forms, with a similar structure but different absorption spectra. The differences are related to point changes in the primary amino acid sequence, but the molecular-level relationship between these cha...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/s11120-018-0492-1

    authors: Nottoli M,Jurinovich S,Cupellini L,Gardiner AT,Cogdell R,Mennucci B

    更新日期:2018-08-01 00:00:00

  • Properties of a pigment system consisting of carotenoids and reaction centre pigments in chromatophores of Rhodospirillum rubrum.

    abstract::A pigment system containing carotenoids and oxidised reaction centre pigments is present in chromatophores of Rhodospirillum rubrum and this pigment system may cause fluorescence quenching when a still unidentified chromatophore component is in its oxidised state. Besides by its action spectrum, this pigment system is...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/BF00018376

    authors: Goedheer JC

    更新日期:1984-03-01 00:00:00

  • Spectral and kinetic characterization of electron acceptor A1 in a Photosystem I core devoid of iron-sulfur centers F X, F B and F A.

    abstract::The kinetic and spectroscopic properties of the secondary electron acceptor A1 were determined by flash absorption spectroscopy at room and cryogenic temperatures in a Photosystem I (PS I) core devoid of the iron-sulfur clusters FX, FB and FA. It was shown earlier (Warren, P.V., Golbeck, J.H. and Warden, J.T. (1993) B...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/BF00015559

    authors: Brettel K,Golbeck JH

    更新日期:1995-09-01 00:00:00

  • Temperature responses of the Rubisco maximum carboxylase activity across domains of life: phylogenetic signals, trade-offs, and importance for carbon gain.

    abstract::Temperature response of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) catalytic properties directly determines the CO2 assimilation capacity of photosynthetic organisms as well as their survival in environments with different thermal conditions. Despite unquestionable importance of Rubisco, the comprehensi...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/s11120-014-0067-8

    authors: Galmés J,Kapralov MV,Copolovici LO,Hermida-Carrera C,Niinemets Ü

    更新日期:2015-02-01 00:00:00

  • Monitoring Migration and Measuring Biomass in Benthic Biofilms: The Effects of Dark/far-red Adaptation and Vertical Migration on Fluorescence Measurements.

    abstract::Pulse modulated fluorescence has increasingly been used as an ecological tool to examine changes in the vertical distribution of microphytobenthic cells within the upper layers of estuarine sediments (most often using the minimum fluorescence yield F(o)) as well as to indicate the health of the community (using the ma...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1023/B:PRES.0000028397.86495.b5

    authors: Consalvey M,Jesus B,Perkins RG,Brotas V,Underwood GJ,Paterson DM

    更新日期:2004-01-01 00:00:00

  • An invitation to the 16th international congress on photosynthesis research in 2013: opportunities and challenges in the 21st century.

    abstract::The 16th International Congress on Photosynthesis will be held August 11-16, 2013 in St. Louis, Missouri, USA. The congress will include 15 plenary lectures, 21 scientific symposia, poster sessions, exhibitors, opening reception, final banquet, excursions and accompanying persons program. The congress is organized as ...

    journal_title:Photosynthesis research

    pub_type:

    doi:10.1007/s11120-013-9847-9

    authors: Blankenship RE,Musick J,Cooley J,Dutcher S,Govindjee

    更新日期:2013-07-01 00:00:00

  • Current status of the role of Cl(-) ion in the oxygen-evolving complex.

    abstract::This minireview summarizes the current state of knowledge concerning the role of Cl(-) in the oxygen-evolving complex (OEC) of photosystem II (PSII). The model that proposes that Cl(-) is a Mn ligand is discussed in light of more recent work. Studies of Cl(-) specificity, stoichiometry, kinetics, and retention by extr...

    journal_title:Photosynthesis research

    pub_type: 杂志文章,评审

    doi:10.1007/s11120-006-9121-5

    authors: Popelková H,Yocum CF

    更新日期:2007-07-01 00:00:00

  • Induction events and short-term regulation of electron transport in chloroplasts: an overview.

    abstract::Regulation of photosynthetic electron transport at different levels of structural and functional organization of photosynthetic apparatus provides efficient performance of oxygenic photosynthesis in plants. This review begins with a brief overview of the chloroplast electron transport chain. Then two noninvasive bioph...

    journal_title:Photosynthesis research

    pub_type: 杂志文章,评审

    doi:10.1007/s11120-015-0094-0

    authors: Tikhonov AN

    更新日期:2015-08-01 00:00:00

  • Comparison of photosystem II complexes isolated from tobacco and two chlorophyll deficient tobacco mutants.

    abstract::A comparative study of photosystem II complexes isolated from tobacco (Nicotiana tabacum L. cv. John William's Broadleaf) which contains normal stacked thylakoid membranes, and from two chlorophyll deficient tobacco mutants (Su/su and Su/su var. Aurea) which have low stacked grana or essentially unstacked thylakoids w...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/BF00032264

    authors: Specht S,Pistorius EK,Schmid GH

    更新日期:1987-01-01 00:00:00

  • Tuning the redox potential of tyrosine-histidine bioinspired assemblies.

    abstract::Photosynthesis powers our planet and is a source of inspiration for developing artificial constructs mimicking many aspects of the natural energy transducing process. In the complex machinery of photosystem II (PSII), the redox activity of the tyrosine Z (Tyrz) hydrogen-bonded to histidine 190 (His190) is essential fo...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/s11120-020-00815-x

    authors: Odella E,Moore TA,Moore AL

    更新日期:2021-01-11 00:00:00

  • Radiationless transitions as a protection mechanism against photoinhibition in higher plants and a red alga.

    abstract::Exposure of the red alga Porphyra perforata or leaves of Phytolacca americana and Echinodorus sp. to white light equivalent to full sunlight for short periods induced large decreases of variable fluorescence measured at 695 nm at 77K. This change was not produced by photoinhibition but rather appeared to result from a...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/BF00118298

    authors: Fork DC,Bose S,Herbert SK

    更新日期:1986-01-01 00:00:00

  • Photosynthetic glow peaks and their relationship with the free energy changes.

    abstract::This paper is concerned with relating thermoluminescence to the total free-energy change, ΣG, involved in detrapping a particular electron-hole pair as a photosynthetic sample is warmed from an initial low temperature. It extends a mathematical discussion of four possible mechanisms introduced in an earlier paper [DeV...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/BF00032597

    authors: Devault D,Govindjee

    更新日期:1990-05-01 00:00:00

  • Energy transfer and pigment composition in three chlorophyll b-containing light-harvesting complexes isolated from Mantoniella squamata (Prasinophyceae), Chlorella fusca (Chlorophyceae) and Sinapis alba.

    abstract::Light-harvesting Chl a/b protein complexes were isolated from the higher plant Sinapis alba, the green alga Chlorella fusca, and the prasinophycean alga Mantoniella squamata by mild gel electrophoresis. The energy transfer from chlorophyll b and the accessory xanthophyll was measured by means of fluoresence spectrosco...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/BF00035234

    authors: Wilhelm C,Lenarz-Weiler I

    更新日期:1987-01-01 00:00:00

  • Vyacheslav (Slava) Klimov (1945-2017): A scientist par excellence, a great human being, a friend, and a Renaissance man.

    abstract::Vyacheslav Vasilevich (V.V.) Klimov (or Slava, as most of us called him) was born on January 12, 1945 and passed away on May 9, 2017. He began his scientific career at the Bach Institute of Biochemistry of the USSR Academy of Sciences (Akademy Nauk (AN) SSSR), Moscow, Russia, and then, he was associated with the Insti...

    journal_title:Photosynthesis research

    pub_type: 传,历史文章,杂志文章

    doi:10.1007/s11120-017-0440-5

    authors: Allakhverdiev SI,Zharmukhamedov SK,Rodionova MV,Shuvalov VA,Dismukes C,Shen JR,Barber J,Samuelsson G,Govindjee

    更新日期:2018-04-01 00:00:00

  • C-type cytochromes in the photosynthetic electron transfer pathways in green sulfur bacteria and heliobacteria.

    abstract::Green sulfur bacteria and heliobacteria are strictly anaerobic phototrophs that have homodimeric type 1 reaction center complexes. Within these complexes, highly reducing substances are produced through an initial charge separation followed by electron transfer reactions driven by light energy absorption. In order to ...

    journal_title:Photosynthesis research

    pub_type: 杂志文章,评审

    doi:10.1007/s11120-009-9521-4

    authors: Azai C,Tsukatani Y,Itoh S,Oh-oka H

    更新日期:2010-06-01 00:00:00

  • In situ c(4) phosphoenolpyruvate carboxylase activity and kinetic properties in isolated digitaria sanguinalis mesophyll cells.

    abstract::Isolated mesophyll cells from darkened leaves of the C(4) plant Digitaria sanguinalis keep functional plasmodesmata that allow the free exchange of low molecular mass compounds with the surrounding medium. This cell suspension system has been used to measure C(4) PEPC activity in situ using a spectrophotometric assay....

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1023/B:PRES.0000017179.31351.f0

    authors: Pierre JN,Prieto JL,Gadal P,Vidal J

    更新日期:2004-01-01 00:00:00

  • The effect of high-energy-state excitation quenching on maximum and dark level chlorophyll fluorescence yield.

    abstract::The quenching of variable fluorescence yield (qN) and the quenching of dark level fluorescence yield (q0) directly atributable to high-energy-state fluorescence quenching (qE) was studied to distinguish between energy dissipation in the antenna and light harvesting complexes (antenna quenching) and energy dissipation ...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/BF00033161

    authors: Rees D,Noctor GD,Horton P

    更新日期:1990-09-01 00:00:00