Abstract：
:The kinetic and spectroscopic properties of the secondary electron acceptor A1 were determined by flash absorption spectroscopy at room and cryogenic temperatures in a Photosystem I (PS I) core devoid of the iron-sulfur clusters FX, FB and FA. It was shown earlier (Warren, P.V., Golbeck, J.H. and Warden, J.T. (1993) Biochemistry 32: 849-857) that the majority of the flash-induced absorbance increase at 820 nm, reflecting formation of P700(+), decays with a t1/2 of 10 μs due to charge recombination between P700(+) and A1 (-). Following A1 (-) directly around 380 nm, where absorbance changes due to the formation of P700(+) are negligible, two major decay components were resolved in this study with t1/2 of ≈ 10 μs and 110 μs at an amplitude ratio of ≈ 2.5:1. The difference spectra between 340 and 490 nm of the two kinetic phases are highly similar, showing absorbance increases from 340 to 400 nm characteristic of the one-electron reduction of the phylloquinone A1. When measured at 10 K, the flash-induced absorbance changes around 380 nm can be fitted with two decay phases of t1/2 ≈ 15 μs and 150 μs at an amplitude ratio ≈ 1:1. The difference spectra of both kinetic phases from 340 to 400 nm are similar to those determined at 298 K and are therefore attributed to charge recombination in the pair P700(+)A1 (-). These results indicate that the backreaction between P700(+) and A1 (-) is multiphasic when FX, FB and FA are removed, and only slightly temperature dependent in the range of 298 K to 10 K.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Brettel K,Golbeck JHdoi
10.1007/BF00015559subject
Has Abstractpub_date
1995-09-01 00:00:00pages
183-93issue
3eissn
0166-8595issn
1573-5079journal_volume
45pub_type
杂志文章abstract：:Chlorophyll fluorescence imaging has been used to analyse the response elicited in Phaseolus vulgaris after inoculation with Pseudomonas syringae pv. phaseolicola 1448A (compatible interaction) and P. syringae pv. tomato DC3000 (incompatible interaction). With the aim of modulating timing of symptom development, diffe...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-007-9278-6
更新日期：2008-04-01 00:00:00
abstract：:P700 enriched fractions were isolated from two brown algae and one diatom using sucrose density centrifugation after digitinin solubilization. They had a Chl a/P700 ratio of about 250 to 375 according to the species, they were enriched in long-wavelength absorbing Chl a and exhibited a fluorescence emission maximum at...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00035009
更新日期：1990-02-01 00:00:00
abstract：:This article gives an historical overview of our group's research on various metabolite translocators of chloroplasts, such as the translocators for phosphorylated intermediates of the Calvin-Benson cycle and of glycolysis, of ADP and ATP, of dicarboxylates, of pyruvate and of hexoses; how it began and where it led to...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1020475803784
更新日期：2002-01-01 00:00:00
abstract：:Redox dependent protein phosphorylation in chloroplast thylakoids regulates distribution of excitation energy between the two photosystems of photosynthesis, PS I and PS II. Several thylakoid phosphoproteins are known to be phosphorylated on N-terminal threonine residues exposed to the chloroplast stroma. Phosphorylat...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1011891017067
更新日期：2001-01-01 00:00:00
abstract：:Photosynthetic organisms normally endeavor to optimize the efficiency of their light-harvesting apparatus. However, here we describe two bacterial isolates belonging to the genera Allochromatium and Thiocapsa that demonstrate a novel adaptation by optimizing their external growth conditions at the expense of photosynt...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-007-9246-1
更新日期：2008-02-01 00:00:00
abstract：:Chlorobaculum [Cba.] tepidum is known to grow optimally at 48-52 °C and can also be cultured at ambient temperatures. In this paper, we prepared constant temperature, temperature shift, and temperature shift followed by backshift cultures and investigated the intrinsic properties and spectral features of chlorosomes f...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-013-9800-y
更新日期：2013-05-01 00:00:00
abstract：:Peridinin-chlorophyll-protein (PCP), containing differently absorbing chlorophyll derivatives, are good models with which to study energy transfer among monomeric chlorophylls (Chls) by both bulk and single-molecule spectroscopy. They can be obtained by reconstituting the N-terminal domain of the protein (N-PCP) with ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-007-9277-7
更新日期：2008-02-01 00:00:00
abstract：:Cyanobacteria, algae, and plants produce dioxygen from water. Driven and clocked by light quanta, the catalytic Mn(4)Ca Tyrosine centre accumulates four oxidizing equivalents before it abstracts four electrons from water and liberates dioxygen and protons. Intermediates of this reaction cascade are short-lived (<100 m...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-008-9321-2
更新日期：2008-10-01 00:00:00
abstract：:We present here the life and work of Shmuel Malkin (1934-2017), an accomplished scientist and a gifted musician who touched the lives of many around the world. His early scientific work addressed the dynamics of light harvesting and electron transport in photosynthesis. Later, he used photoacoustic and photothermal me...
journal_title：Photosynthesis research
pub_type： 传,历史文章,杂志文章
doi：10.1007/s11120-018-0478-z
更新日期：2018-07-01 00:00:00
abstract：:A synthetic fluorochromous lipid, rhodaminyl triglyceride (rhodaminyl TG), was intercalated into isolated thylakoid membranes of chloroplasts up to 30 molecules per 100 molecules of chlorophyll. As a result of fluorochrome presence, an absorption band appeared in a yellow-green spectrum region, its intensity being com...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00047079
更新日期：1990-11-01 00:00:00
abstract：:Chlorophyll f is a photosynthetic pigment that was discovered in 2010. In this study, we present investigations on spectral and dynamic characteristics of singlet-excited and triplet states of Chl f with the application of ultrafast time-resolved absorption and fluorescence spectroscopies. The pigment was studied at r...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-014-9981-z
更新日期：2014-07-01 00:00:00
abstract：:Regulation of photosynthetic electron transport at different levels of structural and functional organization of photosynthetic apparatus provides efficient performance of oxygenic photosynthesis in plants. This review begins with a brief overview of the chloroplast electron transport chain. Then two noninvasive bioph...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-015-0094-0
更新日期：2015-08-01 00:00:00
abstract：:The temperature dependence of the partial reactions leading to turn-over of the UQH2:cyt c 2 oxidoreductase of Rhodobacter sphaeroides have been studied. The redox properties of the cytochrome components show a weak temperature dependence over the range 280-330 K, with coefficients of about 1 m V per degree; our resul...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00114768
更新日期：1989-01-01 00:00:00
abstract：:Flash-induced absorbance measurements at 830 nm on both nanosecond and microsecond timescales have been used to characterise the effect of ultraviolet light on Photosystem II core particles. A combination of UV-A and UV-B, closely simulating the spectrum of sunlight below 350 nm, was found to have a primary effect on ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00029424
更新日期：1996-07-01 00:00:00
abstract：:The crystallization of a given protein is a hard task being even more complicated when the protein shows a hydrophobic behavior. In the case of photosynthetic proteins, the difficulty of the experiments increased due to the high light sensitivity. Aqueous solutions of photosystem II core complex (OEC PSII) of Pisum sa...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-007-9131-y
更新日期：2006-12-01 00:00:00
abstract：:The D1/D2 heterodimer core is the heart of the photosystem II reaction center. A characteristic feature of this heterodimer is the differentially rapid, light-dependent degradation of the D1 protein. The D1 protein is possibly the most researched photosynthetic polypeptide, with aspects of structure-function, gene, me...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-008-9342-x
更新日期：2008-10-01 00:00:00
abstract：:The pigment composition of the light-harvesting complexes of Photosystem II (LHC II) has been determined for lettuce (Lactuca sativa). In common with other members of the composite, the photosynthetic tissues of this species may contain large amounts of the carotenoid lactucaxanthin (ε, ε-carotene-3,3'-diol) in additi...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00029940
更新日期：1995-03-01 00:00:00
abstract：:This work addresses the question of occurrence and function of photosystem II (PSII) in bundle sheath (BS) cells of leaves possessing NADP-malic enzyme-type C4 photosynthesis (Zea mays). Although no requirement for PSII activity in the BS has been established, several component proteins of PSII have been detected in B...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-014-0009-5
更新日期：2014-10-01 00:00:00
abstract：:Roderick K. Clayton passed away on October 23, 2011, at the age of 89, shortly after the plan for this dedicatory issue of Photosynthesis Research had been hatched. I had just written a lengthy letter to him to re-establish contact after a hiatus of 2 or 3 years, and to suggest that I visit him to talk about his life....
journal_title：Photosynthesis research
pub_type： 传,历史文章,杂志文章
doi：10.1007/s11120-013-9948-5
更新日期：2014-05-01 00:00:00
abstract：:Phosphatidylglycerol (PG), containing the unique fatty acid Delta3, trans-16:1-hexadecenoic acid, is a minor but ubiquitous lipid component of thylakoid membranes of chloroplasts and cyanobacteria. We investigated its role in electron transfers and structural organization of Photosystem II (PSII) by treating Arabidops...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-006-9126-0
更新日期：2007-07-01 00:00:00
abstract：:Two portable instruments, designed to evaluate epidermal UV screening in leaves, were compared: the Dualex and the UV-A-PAM fluorimeter. Both instruments excite chlorophyll fluorescence at the same UV wavelengths but reference excitation is in the red and the blue spectral range in the former and the latter fluorimete...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-007-9135-7
更新日期：2007-07-01 00:00:00
abstract：:In thylakoid membranes, several peptides of high MW† are present which may interfere with the study of CP1's components. Modifying Cleveland's technique [7] for limited proteolysis, we have characterized the polypeptides found in the 60 kD region. Some may result from incomplete washing of the CF1 while others come fr...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00020071
更新日期：1980-03-01 00:00:00
abstract：:A combination of inhibitor and (15)N studies were used to investigate the photorespiratory nitrogen cycle in maize, a C4 plant. Inhibitors used included isonicotinyl hydrazide which blocks the conversion of glycine to serine, methionine sulfoximine an inhibitor of GS and azaserine an inhibitor of GOGAT. Results from l...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00041796
更新日期：1983-01-01 00:00:00
abstract：:We have used antibodies generated against synthetic peptides to determine the topology of the 43 kD chlorophyll a binding protein (CP 43) of Photosystem II. Based on the pattern of proteolytic fragments detected (on western blots) by peptide specific antibodies, a six transmembrane span topological model, with the ami...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00019041
更新日期：1994-04-01 00:00:00
abstract：:Dan Arnon, Bob Whatley, Mary Belle Allen, and their colleagues, were the first to obtain evidence for 'complete photosynthesis by isolated chloroplasts' albeit at rates which were 1% or less of those displayed by the intact leaf. By the 1960s, partly in the hope of confirming full functionality, there was a perceived ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1024962328483
更新日期：2003-01-01 00:00:00
abstract：:Since 1986, non-uniform photosynthesis over the leaf area that may be attributed to patchy stomatal closure, has been an important issue in stress physiology of photosynthesis. In this review, I first outline the gaseous environment within the intercellular spaces, because this is the most fundamental background of th...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00035537
更新日期：1992-03-01 00:00:00
abstract：:Pulse modulated fluorescence has increasingly been used as an ecological tool to examine changes in the vertical distribution of microphytobenthic cells within the upper layers of estuarine sediments (most often using the minimum fluorescence yield F(o)) as well as to indicate the health of the community (using the ma...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/B:PRES.0000028397.86495.b5
更新日期：2004-01-01 00:00:00
abstract：:There is diverse, yet controversial fossil evidence for the existence of photosynthesis 3500 million years ago. Among the most persuasive evidence is the stromatolites described from low grade metasedimentary rocks in Western Australia and South Africa. Based on the understanding of the paleobiology of stromatolites a...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00039172
更新日期：1992-08-01 00:00:00
abstract：:The unicellular green alga Chlamydomonas reinhardtii acclimates to low-CO2 (LC) conditions by actively transporting inorganic carbon (Ci) into the cell, resulting in an increase in photosynthetic efficiency. This mechanism is called the carbon-concentrating mechanism (CCM), and soluble protein LCIB is essential for th...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-013-9963-6
更新日期：2014-09-01 00:00:00
abstract：:In vitro studies of the carotenoid peridinin, which is the primary pigment from the peridinin chlorophyll-a protein (PCP) light harvesting complex, showed a strong dependence on the lifetime of the peridinin lowest singlet excited state on solvent polarity. This dependence was attributed to the presence of an intramol...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-005-1447-x
更新日期：2005-11-01 00:00:00
