Abstract：
:In thylakoid membranes, several peptides of high MW† are present which may interfere with the study of CP1's components. Modifying Cleveland's technique [7] for limited proteolysis, we have characterized the polypeptides found in the 60 kD region. Some may result from incomplete washing of the CF1 while others come from the CP1; indeed, this chlorophyll protein complex, which has a higher MW (above 100 kD), very often undergoes a dissociation into smaller components of about 60 KD MW.Analysis of the protein content of different preparations commonly used to obtain PSI reaction centre enriched fractions has been performed. The α and β subunits of CF1 are among the main contaminants of most of these preparations. A further purification step is described which can be applied to all these preparations, but numerous peptides are still present in the active fractions. It is most unlikely that all these polypeptides are required for the primary photochemical event, and this emphasizes the necessity to find a new simple method to purify PSI reaction centres.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Lagoutte B,Setif P,Duranton Jdoi
10.1007/BF00020071subject
Has Abstractpub_date
1980-03-01 00:00:00pages
3-16issue
1eissn
0166-8595issn
1573-5079journal_volume
1pub_type
杂志文章abstract：:Since 1986, non-uniform photosynthesis over the leaf area that may be attributed to patchy stomatal closure, has been an important issue in stress physiology of photosynthesis. In this review, I first outline the gaseous environment within the intercellular spaces, because this is the most fundamental background of th...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00035537
更新日期：1992-03-01 00:00:00
abstract：:Photoacclimation by strains of Haslea "blue" diatom species H. ostrearia and H. silbo sp. nov. ined. was investigated with rapid light curves and induction-recovery curves using fast repetition rate fluorescence. Cultures were grown to exponential phase under 50 µmol m-2 s-1 photosynthetic available radiation (PAR) an...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-018-0508-x
更新日期：2018-09-01 00:00:00
abstract：:Sulphoquinovosyldiacylglycerol is a negatively charged lipid which exists in the thylakoid membrane. It is proposed that a large proportion of this acidic lipid does not form a part of the bulk lipid matrix but is closely associated with protein complexes where it is tightly bound and participates in either optimising...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00029747
更新日期：1986-01-01 00:00:00
abstract：:The 16th International Congress on Photosynthesis will be held August 11-16, 2013 in St. Louis, Missouri, USA. The congress will include 15 plenary lectures, 21 scientific symposia, poster sessions, exhibitors, opening reception, final banquet, excursions and accompanying persons program. The congress is organized as ...
journal_title：Photosynthesis research
pub_type：
doi：10.1007/s11120-013-9847-9
更新日期：2013-07-01 00:00:00
abstract：:In this Minireview, we discuss a number of issues on the primary photosynthetic reactions of the green plant Photosystem II. We discuss the origin of the 683 and 679 nm absorption bands of the PS II RC complex and suggest that these forms may reflect the single-site spectrum with dominant contributions from the zero-p...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1006468024245
更新日期：2000-01-01 00:00:00
abstract：:Solubilisation of thylakoid membranes from young leaves of Pisum sativum in the presence of Triton X-100 resulted in an almost complete loss of quenching of light-harvesting chlorophyll-protein (LHCP) fluorescence, as measured at 77°K. There were concomitant changes in the kinetics of light-saturation curves of electr...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00037130
更新日期：1986-01-01 00:00:00
abstract：:Modulation of the efficiency with which leaves convert absorbed light to photochemical energy [intrinsic efficiency of open photosystem II (PSII) centers, as the ratio of variable to maximal chlorophyll fluorescence] as well as leaf xanthophyll composition (interconversions of the xanthophyll cycle pigments violaxanth...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-012-9761-6
更新日期：2012-09-01 00:00:00
abstract：:The oxygen flash yield (YO2) and photochemical yield of PS II (ΦPS II) were simultaneously detected in intact Chlorella cells on a bare platinum oxygen rate electrode. The two yields were measured as a function of background irradiance in the steady-state and following a transition from light to darkness. During stead...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00029472
更新日期：1996-06-01 00:00:00
abstract：:Bioenergetic properties of thylakoids from plants submitted to a water stress stress (watering stopped for 6-15 days) have been measured in two lupin genotypes characterized as resistant or susceptible to drought. This energy coupling was assessed by flow-force relationships relating the phosphorylation rate to the ma...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00035944
更新日期：1992-05-01 00:00:00
abstract：:Free ribulose hisphosphate (RuBP(4-)) rather than its magnesium complex (RuBP-Mg(2-)) was the apparent substrate for spinach ribulose bisphosphate carboxylase/oxygenase. The apparent Km for total RuBP (pH 8.0 at 30° C) increased with increasing Mg(2+) concentrations from 11.6 μM at 13.33 mM Mg(2+) to 32.6 μM at 40.33 ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00041806
更新日期：1983-01-01 00:00:00
abstract：:While interest in photosynthetic thermal acclimation has been stimulated by climate warming, comparing results across studies requires consistent terminology. We identify five types of photosynthetic adjustments in warming experiments: photosynthesis as measured at the high growth temperature, the growth temperature, ...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-013-9873-7
更新日期：2014-02-01 00:00:00
abstract：:The chloroplast genome of the highly neutral-lipid-producing marine pennate diatom Fistulifera sp. strain JPCC DA0580 was fully sequenced using high-throughput pyrosequencing. The general features and gene content were compared with three other complete diatom chloroplast genomes. The chloroplast genome is 134,918 bp ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-011-9622-8
更新日期：2011-09-01 00:00:00
abstract：:Previous investigations have shown that increased source strength as a result of elevated CO(2) can alter the timing of the phases of change that occur in photosynthetic rates during dicot leaf ontogeny [Miller et al. (1997) Plant Physiol 115: 1195-1200]. To evaluate the converse situation of decreased source strength...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1006367719639
更新日期：2000-01-01 00:00:00
abstract：:The problem of predicting the kinetics of proton efflux and the decay of the internal proton concentration for vesicles containing one or more buffers for which the internal proton concentration is initially higher than that of the surrounding medium is examined. An analytical solution is derived that describes the ti...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00019150
更新日期：1987-01-01 00:00:00
abstract：:A detailed analysis of the periplasmic electron carriers of the photosynthetic bacterium Ectothiorhodospira sp. has been performed. Two low mid-point redox potential electron carriers, cytochrome c' and cytochrome c, are detected. A high potential iron-sulfur protein is the only high mid-point redox potential electron...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1006486307473
更新日期：2000-01-01 00:00:00
abstract：:The study of the light-harvesting complex II (LHC-II) phosphatase activity has been difficult due to the membrane association of its substrate. Thylakoid membranes labeled with [γ-(32)P]ATP were incubated with chymotrypsin, releasing phosphopeptides which served as labeled substrates for LHC-II phosphatase. Utilizing ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00018301
更新日期：1995-05-01 00:00:00
abstract：:The functional peculiarities and responses of the photosynthetic system in the flowering homoiochlorophyllous desiccation-tolerant (HDT) Haberlea rhodopensis and the non-desiccation-tolerant spinach were compared during desiccation and rehydration. Increasing rate of water loss clearly modifies the kinetic parameters ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-005-2440-0
更新日期：2005-08-01 00:00:00
abstract：:The activity of ribulose-1,5-bisphosphate carboxylase/oxygenase fromEuglena gracilis decays steadily when exposed to agents that induce oxidative modification of cysteine residues (Cu(2+), benzofuroxan, disulfides, arsenite, oxidized ascorbate). Inactivation takes place with a concomitant loss of cysteine sulfhydryl g...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF02185411
更新日期：1993-01-01 00:00:00
abstract：:Photoinhibition of Photosystem II (PSII) in lincomycin-treated leaves begins as a first-order reaction, but fluorescence measurements have suggested that after prolonged illumination, the number of active PSII centres stabilizes to 15-20% of control. The stabilization has been interpreted to indicate that photoinhibit...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-009-9496-1
更新日期：2010-01-01 00:00:00
abstract：:Species in the Laxa and Grandia groups of the genus Panicum are adapted to low, wet areas of tropical and subtropical America. Panicum milioides is a species with C3 photosynthesis and low apparent photorespiration and has been classified as a C3/C4 intermediate. Other species in the Laxa group are C3 with normal phot...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00024189
更新日期：1986-01-01 00:00:00
abstract：:Fourier transform infrared (FTIR) spectroscopy probes the vibrational properties of amino acids and cofactors, which are sensitive to minute structural changes. The lack of specificity of this technique, on the one hand, permits us to probe directly the vibrational properties of almost all the cofactors, amino acid si...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-009-9439-x
更新日期：2009-08-01 00:00:00
abstract：:In vitro studies of the carotenoid peridinin, which is the primary pigment from the peridinin chlorophyll-a protein (PCP) light harvesting complex, showed a strong dependence on the lifetime of the peridinin lowest singlet excited state on solvent polarity. This dependence was attributed to the presence of an intramol...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-005-1447-x
更新日期：2005-11-01 00:00:00
abstract：:I provide here a personal perspective on (i) the awards that were given to young investigators at the 2008 and 2009 Gordon Research Conferences on Biochemistry/Biophysics of Photosynthesis; and (ii) the ambiance at these conferences through some photographs, particularly of the 2009 conference. ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-009-9479-2
更新日期：2009-10-01 00:00:00
abstract：:The 3- and 7-formyl groups of chlorophyll-d (Chl-d) and bacteriochlorophyll-e (BChl-e), respectively, were regioselectively labeled with an isotopically stable oxygen-18 (¹⁸O) atom to give 3¹-¹⁸O-labeled Chl-d and 7¹-¹⁸O-labeled BChl-e (ca. 90% ¹⁸O) by exchanging the carbonyl oxygen atoms in the presence of acidic H₂ ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-010-9590-4
更新日期：2010-09-01 00:00:00
abstract：:The effect of desiccation and rehydration on the function of Photosystem II has been studied in the desiccation tolerant lichen Cladonia convoluta by thermoluminescence. We have shown that in functional fully hydrated thalli thermoluminescence signals can be observed from the recombination of the S2(3)QB (-) (B band),...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00041010
更新日期：1996-05-01 00:00:00
abstract：:The linear, four-step oxidation of water to molecular oxygen by photosystem II requires cooperation between redox reactions driven by light and a set of redox reactions involving the S-states within the oxygen-evolving complex. The oxygenevolving complex is a highly ordered structure in which a number of polypeptides ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00037001
更新日期：1985-01-01 00:00:00
abstract：:A chloroplast preparation was extracted from squash (Cucurbita pepo (L.) var. Senator). Enrichment of intact chloroplasts was achieved by continuous free-flow electrophoresis. The addition of monoterpenes, detergent and free fatty acids changed the elecrophoretic separation pattern characteristically. Monoterpene-depe...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00054124
更新日期：1991-06-01 00:00:00
abstract：:Photosynthesis was well-established on the earth at least 3.5 thousand million years ago, and it is widely believed that these ancient organisms had similar metabolic capabilities to modern cyanobacteria. This requires that development of two photosystems and the oxygen evolution capability occurred very early in the...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：
更新日期：1992-01-01 00:00:00
abstract：:The nucleotide sequences of the genes coding for the subunits of the Photosystem I (PS I) core, PsaA and PsaB were determined for the marine prokaryotic oxyphototrophs Prochlorococcus sp. MED4 (CCMP1378), P. marinus SS120 (CCMP1375) and Synechococcus sp. WH7803. Divergence of these sequences from those of both freshwa...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1006445810996
更新日期：2000-01-01 00:00:00
abstract：:Historically, two main concepts guided research into possible mechanisms of light-induced atomic rearrangements in oxygenic photosynthesis: Photodecomposition of CO2 and photodecomposition of water. Both concepts envisioned photoinduced transfers of cumbersome whole atoms and not, as is currently held, photoinduced el...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00036216
更新日期：1991-09-01 00:00:00
