Abstract:
:The study of the light-harvesting complex II (LHC-II) phosphatase activity has been difficult due to the membrane association of its substrate. Thylakoid membranes labeled with [γ-(32)P]ATP were incubated with chymotrypsin, releasing phosphopeptides which served as labeled substrates for LHC-II phosphatase. Utilizing these phosphopeptides as substrates, protein phosphatase activities have been identified in both the thylakoid membrane and the stromal fraction. The thylakoid-bound phosphatase was liberated from the membrane with a sub-solubilizing concentration of Brij 35. The membrane and the stromal protein phosphatases were inhibited by NaF and EDTA, but not inhibited by microcystin-LR. The stromal phosphatase differed from the membrane phosphatase in pH optimum, in its lack of inhibition by molybdate ions, and by its response to magnesium and manganese ions. Using the soluble chymotryptic peptide substrate, the effect of light on pea thylakoid-bound LHC-II phosphatase activity was also assessed. Incubation of the thylakoid membranes in the light caused a 35% inhibition of LHC-II phosphatase activity. The inhibition was diminished by the addition of DCMU. Addition of 10 mM dithiothreitol stimulated the activity in darkness and obviated the inhibition when exposed to light. These studies suggest that positive or negative regulation of the LHC-II phosphatase activity is possible in vivo.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Hammer MF,Sarath G,Osterman JC,Markwell Jdoi
10.1007/BF00018301subject
Has Abstractpub_date
1995-05-01 00:00:00pages
107-15issue
1-2eissn
0166-8595issn
1573-5079journal_volume
44pub_type
杂志文章abstract::Tropical plants are sensitive to chilling temperatures above zero but it is still unclear whether photosystem I (PSI) or photosystem II (PSII) of tropical plants is mainly affected by chilling temperatures. In this study, the effect of 4 degrees C associated with various light densities on PSII and PSI was studied in ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-010-9539-7
更新日期:2010-03-01 00:00:00
abstract::Bill Arnold's concept of photosynthesis as a solid state, as opposed to solution, phenomenon led him to an amazing series of fundamental discoveries. ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00040990
更新日期:1996-05-01 00:00:00
abstract::In appreciation of his contribution to the Photosystsem II research and commemoration of the book Photosystem II: The Light-Driven Water-Plastoquinone Oxido-Reductase, co-edited with Kimiyuki Satoh, I present here some of my recollections of Thomas John Wydrzynski and by several others with whom he has associated over...
journal_title:Photosynthesis research
pub_type: 传,历史文章,杂志文章
doi:10.1007/s11120-008-9341-y
更新日期:2008-10-01 00:00:00
abstract::Chlorophyll fluorescence imaging has been used to analyse the response elicited in Phaseolus vulgaris after inoculation with Pseudomonas syringae pv. phaseolicola 1448A (compatible interaction) and P. syringae pv. tomato DC3000 (incompatible interaction). With the aim of modulating timing of symptom development, diffe...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-007-9278-6
更新日期:2008-04-01 00:00:00
abstract::Oxygen evolving photosystem II particles were exposed to 100 and 250 W m(-2) white light at 20°C under aerobic, anaerobic and strongly reducing (presence of dithionite) conditions. Three types of photoinactivation processes with different kinetics could be distinguished: (1) The fast process which occurs under strongl...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00030061
更新日期:1990-01-01 00:00:00
abstract::Four Indica and five Japonica varieties of rice (Oryza sativa L.) were examined to elucidate their differences in photosynthetic activity and dark respiratory rate as influenced by leaf nitrogen levels and temperatures. The photosynthetic rates of single leaf showed correlations with total nitrogen and soluble protein...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00032321
更新日期:1987-01-01 00:00:00
abstract::Dry weight and Relative Growth Rate of Lemna gibba were significantly increased by CO2 enrichment up to 6000 μl CO2 l(-1). This high CO2 optimum for growth is probably due to the presence of nonfunctional stomata. The response to high CO2 was less or absent following four days growth in 2% O2. The Leaf Area Ratio decr...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00029048
更新日期:1985-03-01 00:00:00
abstract::Pulse modulated fluorescence has increasingly been used as an ecological tool to examine changes in the vertical distribution of microphytobenthic cells within the upper layers of estuarine sediments (most often using the minimum fluorescence yield F(o)) as well as to indicate the health of the community (using the ma...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/B:PRES.0000028397.86495.b5
更新日期:2004-01-01 00:00:00
abstract::The PAM-2000 portable chlorophyll fluorometer represents one of the first commercially available instruments utilizing the Pulse Amplitude Modulation (PAM) measurement principle, and has become a widely used platform for measuring chlorophyll fluorescence in a wide range of study systems. In this paper, we describe a ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-005-9010-3
更新日期:2006-03-01 00:00:00
abstract::To investigate the impact of iron deficiency on bioenergetic pathways in Chlamydomonas, we compared growth rates, iron content, and photosynthetic parameters systematically in acetate versus CO(2)-grown cells. Acetate-grown cells have, predictably (2-fold) greater abundance of respiration components but also, counter-...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-010-9562-8
更新日期:2010-07-01 00:00:00
abstract::Oxygenic photosynthesis is driven by two multi-subunit membrane protein complexes, Photosystem I and Photosystem II. In plants and green algae, both complexes are composed of two moieties: a reaction center (RC), where light-induced charge translocation occurs, and a peripheral antenna that absorbs light and funnels i...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/B:PRES.0000036881.23512.42
更新日期:2004-01-01 00:00:00
abstract::Minimal photosynthetic catalytic F1(αβ) core complexes, containing equimolar ratios of the α and β subunits, were isolated from membrane-bound spinach chloroplast CF1 and Rhodospirillum rubrum chromatophore RrF1. A CF1-α3β3 hexamer and RrF1-α1β1 dimer, which were purified from the respective F1(αβ) complexes, exhibit ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00020418
更新日期:1995-11-01 00:00:00
abstract::C4 photosynthesis is a complex trait that has a high degree of natural variation, involving anatomical and biochemical changes relative to the ancestral C3 state. It has evolved at least 66 times across a variety of lineages and the evolutionary route from C3 to C4 is likely conserved but not necessarily genetically i...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-013-9872-8
更新日期:2014-02-01 00:00:00
abstract::The cyanobacterial small CAB-like proteins (SCPs) are one-helix proteins with compelling similarity to the first and third transmembrane helix of proteins belonging to the CAB family of light-harvesting complex proteins in plants. The SCP proteins are transiently expressed at high light intensity and other stress cond...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-007-9244-3
更新日期:2008-02-01 00:00:00
abstract::In this communication, evidence is presented from the kinetics of QA (-) decay (where QA is the first plastoquinone electron acceptor of photosystem II) and oxygen evolution for the requirement of bicarbonate in the electron transport in a cyanobacteriumSynechocystis (Pasteur Culture Collection 6803). A large slowing ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00046879
更新日期:1988-10-01 00:00:00
abstract::Modified forms of the D1 protein with deletions in lumen-exposed regions, were constructed in the cyanobacterium Synechocystis 6803 using site-directed mutagenesis. Integration and stability of the mutated D1 proteins in the thylakoid membrane were studied by immunoblot and pulse-chase analyses. It was found that in Δ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00117663
更新日期:1996-08-01 00:00:00
abstract::Little is known about the mechanistic basis for the movement of promiscuous nucleic acids across cell membranes. To address this problem we sought conditions that would permit the entry of plasmid DNA into isolated, intact pea chloroplasts. DNA uptake did not occur normally, but was induced by hypotonic treatments, by...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00020448
更新日期:1995-11-01 00:00:00
abstract::Recently, a number of techniques, some of them relatively new and many often used in combination, have given a clearer picture of the dynamic role of electron transport in Photosystem I of photosynthesis and of coupled cyclic photophosphorylation. For example, the photoacoustic technique has detected cyclic electron t...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00033035
更新日期:1993-06-01 00:00:00
abstract::A hypothesis is proposed to explain the function of Cl(-) in activating the oxygenevolving complex (OEC) of photosystem II (PS II), based on the results of recent (35)Cl-NMR studies. The putative mechanism involves Cl(-) binding to two types of sites. An intrinsic site is suggested to be composed of three histidyl res...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00029400
更新日期:1987-09-01 00:00:00
abstract::Intra- and inter-specific variation in CO2 assimilation rate (A) in Triticum spp. is well documented for reproductive growth stages. Research was conducted to characterize early vegetative photosynthetic variation in a diverse set of cultivated hexaploid wheat (T. aestivum L.) germplasm and in wild tetraploid (T. dico...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00034120
更新日期:1989-05-01 00:00:00
abstract::Light-harvesting Chl a/b protein complexes were isolated from the higher plant Sinapis alba, the green alga Chlorella fusca, and the prasinophycean alga Mantoniella squamata by mild gel electrophoresis. The energy transfer from chlorophyll b and the accessory xanthophyll was measured by means of fluoresence spectrosco...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00035234
更新日期:1987-01-01 00:00:00
abstract::Chloroplast NADP-dependent malate dehydrogenase (NADP-MDH, EC 1.1.1.82) is inactive in the dark and activated in the light via a reduction of specific disulfides by thiol-disulfide interchange with thioredoxin, reduced by the photosynthetic electron transfer. Compared to the constitutively active NAD-dependent forms, ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1016099228450
更新日期:2002-01-01 00:00:00
abstract::Almost 1 year has passed since the death of Wolfgang Haehnel on 13.5.2013, after a long fight against cancer. With his death, the photosynthesis community has lost an excellent scientist, a critical peer, and a stimulating sparring partner in discussions: I also lost a good friend. ...
journal_title:Photosynthesis research
pub_type: 传,历史文章
doi:10.1007/s11120-014-9999-2
更新日期:2014-06-01 00:00:00
abstract::We present here our tribute to an outstanding unique scientist, a pioneer of plant biochemistry, best known for his seminal ground-breaking discoveries related to electron and proton transport, and their intimate relation to ATP synthesis in chloroplasts. His dedication and in-depth understanding of science was matche...
journal_title:Photosynthesis research
pub_type: 传,历史文章,杂志文章
doi:10.1007/s11120-017-0380-0
更新日期:2017-06-01 00:00:00
abstract::The interaction of PsaC with the core heterodimer of Photosystem 1 was studied in wild type Synechocystis sp. PCC 6803 and the site-directed mutant R561E of PsaB. The mutant reaction center was much less stable in urea and the functional reconstitution of the mutant core using PsaC was impaired. However, the extent of...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00018261
更新日期:1994-12-01 00:00:00
abstract::A combination of inhibitor and (15)N studies were used to investigate the photorespiratory nitrogen cycle in maize, a C4 plant. Inhibitors used included isonicotinyl hydrazide which blocks the conversion of glycine to serine, methionine sulfoximine an inhibitor of GS and azaserine an inhibitor of GOGAT. Results from l...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00041796
更新日期:1983-01-01 00:00:00
abstract::The unicellular green alga Chlamydomonas reinhardtii acclimates to low-CO2 (LC) conditions by actively transporting inorganic carbon (Ci) into the cell, resulting in an increase in photosynthetic efficiency. This mechanism is called the carbon-concentrating mechanism (CCM), and soluble protein LCIB is essential for th...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-013-9963-6
更新日期:2014-09-01 00:00:00
abstract::Two-component regulatory systems that respond to changes in redox potential have recently been discovered in bacteria. 'Redox sensors' are defined as electron carriers which initiate control of gene expression upon oxidation or reduction. 'Redox response regulators' are defined as DNA-binding proteins which modify gen...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00016274
更新日期:1993-05-01 00:00:00
abstract::Various techniques of electron microscopy (EM) such as ultrathin sectioning, freeze-fracturing, freeze-etching, negative staining and (cryo-)electron crystallography of two-dimensional crystals have been employed, since now, to obtain much of the structural information of the Photosystem II (PS II) pigment-protein com...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1024927631196
更新日期:2003-01-01 00:00:00
abstract::A new binding site for anions which inhibit the water oxidizing complex (WOC) of Photosystem II in spinach has been identified. Anions which bind to this site inhibit the flash-induced S2/S0 catalase reaction (2H2O2→2H2O+O2) of the WOC by displacing hydrogen peroxide. Using a mass spectrometer and gas permeable membra...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00046771
更新日期:1993-01-01 00:00:00