Abstract：
:Modified forms of the D1 protein with deletions in lumen-exposed regions, were constructed in the cyanobacterium Synechocystis 6803 using site-directed mutagenesis. Integration and stability of the mutated D1 proteins in the thylakoid membrane were studied by immunoblot and pulse-chase analyses. It was found that in Δ(N325-E333), the D1 protein with a deletion in the C-terminal tail, could insert in the thylakoids to normal amounts but its stability in the membrane was dramatically reduced. Insertion of D1 in Δ(V58-D61) or Δ(D103-G109);G110R, with deletions in the A-B loop, was severely obstructed, For Δ(P350-T354), with a deletion in the processed region of the C-terminus of D1, no phenotypic effects were observed. The effects of failed D1 insertion or accumulation on Photosystem II assembly was monitored by immunoblot analysis. The conclusions from these experiments are that the extrinsic 33 kDa protein, CP43, and the β subunit of cytochrome b559 accumulate in the thylakoid membrane independently of the D1 protein, and that accumulation of the D2 protein and CP47 requires insertion but not necessarily accumulation of the D1 protein.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Salih G,Wiklund R,Tyystjärvi T,Mäenpää P,Gerez C,Jansson Cdoi
10.1007/BF00117663subject
Has Abstractpub_date
1996-08-01 00:00:00pages
131-40issue
2eissn
0166-8595issn
1573-5079journal_volume
49pub_type
杂志文章abstract：:Six chlorophyll-protein complexes are isolated from thylakoid membranes of Bryopsis corticulans by dodecyl-beta-D: -maltoside polyacrylamide gel electrophoresis. Unlike that of higher plants, the 77 K fluorescence emission spectrum of the CP1 band, the PSI core complexes of B. corticulans, presents two peaks, one at 6...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-007-9286-6
更新日期：2008-04-01 00:00:00
abstract：:Here, we describe a new imaging setup able to assess in vivo photosynthetic activity. The system specifically measures time-resolved chlorophyll fluorescence in response to light. It is composed of a fast digital camera equipped with a wide-angle lens for the analysis of samples up to 10 x 10 cm, i.e. entire plants or...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-009-9487-2
更新日期：2009-10-01 00:00:00
abstract：:The excitation energy transfer between carotenoid and chlorophyll (Chl) in the cytochrome b ( 6 ) f complex from Bryopsis corticulans (B. corticulans), in which the carotenoid is 9-cis-alpha-carotene, was investigated by means of fluorescence excitation and sub-microsecond time-resolved absorption spectroscopies. The ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-005-9020-1
更新日期：2006-04-01 00:00:00
abstract：:Cassava, bean and maize leaves were fed with(14)CO2 in light and the primary products of photosynthesis identified 5 and 10 seconds after assimilation. In maize, approximately three quarters of the labelled carbon was incorporated in C4 acids, in beans about two thirds in PGA, and in cassava approximately 40-60% in C4...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00055123
更新日期：1987-01-01 00:00:00
abstract：:The afterglow (AG) band of thermoluminescence (TL) has been investigated in leaves of Arabidopsis thaliana. Excitation of dark-adapted leaves with two saturating single turn-over flashes induced the appearance of a complex TL glow curve that could be well simulated by three components: the two components, B1 and B2, o...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-004-7311-6
更新日期：2005-06-01 00:00:00
abstract：:A peptide ranging from residue 229 to 240 of the D1 protein of Photosystem (PS) II was synthesized and lanthanides were used as candidates of calcium. Fluorescence and FTIR spectroscopy were used to test the conformational adaptation after lanthanide additions. Fluorescence spectroscopy showed that the synthetic pepti...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00048603
更新日期：1995-06-01 00:00:00
abstract：:Henrik Lundegårdh made major contributions in the field of ecology and plant physiology from 1912 to 1969. His early work at Hallands Väderö in the Kattegat pioneered quantitative approaches to plant ecology and laid the understanding of carbon dioxide exchange in natural communities which is still useful today in glo...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1024980325118
更新日期：2003-01-01 00:00:00
abstract：:Pigment-protein-complexes of two chlorophyll b deficient mutants of Arabidopsis and from the wild type were separated electrophoretically. Light-harvesting proteins were absent in the chlorophyll b free mutant ch(1) and their amount was reduced in the mutant ch(2) which has a reduced content of chlorophyll b. The rati...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00041827
更新日期：1983-01-01 00:00:00
abstract：:The effect of leaf water potential (Ψ) on net CO2 assimilation rate (A), stomatal conductance (g), transpiration (E) and water-use efficiency (WUE) was measured for three cultivars of cacao (Theobroma cacao L.) seedlings during three recurrent drought cycles. Net assimilation varied greatly at high water potentials, b...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00037179
更新日期：1989-09-01 00:00:00
abstract：:Energy transfer of the light harvesting complex LHC-II trimer, extracted from spinach, was studied in the Q(y) region at room temperature by femtosecond transient absorption spectroscopy. Configuration interaction exciton method [Linnanto et al. (1999) J Phys Chem B 103: 8739-8750] and 2.72 A structural information re...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-005-9004-1
更新日期：2006-03-01 00:00:00
abstract：:Exposure of the red alga Porphyra perforata or leaves of Phytolacca americana and Echinodorus sp. to white light equivalent to full sunlight for short periods induced large decreases of variable fluorescence measured at 695 nm at 77K. This change was not produced by photoinhibition but rather appeared to result from a...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00118298
更新日期：1986-01-01 00:00:00
abstract：:Phosphatidylglycerol (PG), containing the unique fatty acid Delta3, trans-16:1-hexadecenoic acid, is a minor but ubiquitous lipid component of thylakoid membranes of chloroplasts and cyanobacteria. We investigated its role in electron transfers and structural organization of Photosystem II (PSII) by treating Arabidops...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-006-9126-0
更新日期：2007-07-01 00:00:00
abstract：:A protein was isolated from membranes of the green sulfur bacterium Chlorobium tepidum. This protein was characterized by gel electrophoresis, gel filtration, analytical ultracentrifugation and amino acid sequencing. The molecular weight of the purified protein was shown to be 26 kDa by SDS-PAGE. HPLC gelfiltration, S...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/B:PRES.0000015383.58680.56
更新日期：2004-02-01 00:00:00
abstract：:Bill Arnold's concept of photosynthesis as a solid state, as opposed to solution, phenomenon led him to an amazing series of fundamental discoveries. ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00040990
更新日期：1996-05-01 00:00:00
abstract：:The world is facing energy crisis and environmental issues due to the depletion of fossil fuels and increasing CO2 concentration in the atmosphere. Growing microalgae can contribute to practical solutions for these global problems because they can harvest solar energy and capture CO2 by converting it into biofuel usin...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-013-9828-z
更新日期：2013-11-01 00:00:00
abstract：:This review focuses on our recent efforts in synthetic ruthenium-tyrosine-manganese chemistry mimicking the donor side reactions of Photosystem II. Tyrosine and tryptophan residues were linked to ruthenium photosensitizers, which resulted in model complexes for proton-coupled electron transfer from amino acids. A new ...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-005-9005-0
更新日期：2006-01-01 00:00:00
abstract：:Carbohydrate feedstocks are at the root of bioindustrial production and are needed in greater quantities than ever due to increased prioritization of renewable fuels with reduced carbon footprints. Cyanobacteria possess a number of features that make them well suited as an alternative feedstock crop in comparison to t...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-014-9980-0
更新日期：2015-03-01 00:00:00
abstract：:Cuttings of grapevine (Vitis vinifera L. cv. Chardonnay) were dark-forced at least three weeks. Pigment contents, 77 K fluorescence emission, excitation spectra of the leaves, petioles, stems, transmission electron micrographs of the etioplasts from leaves, the chlorenchyma tissues of the stems were analysed. The dark...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-004-1061-3
更新日期：2004-01-01 00:00:00
abstract：:In this communication, evidence is presented from the kinetics of QA (-) decay (where QA is the first plastoquinone electron acceptor of photosystem II) and oxygen evolution for the requirement of bicarbonate in the electron transport in a cyanobacteriumSynechocystis (Pasteur Culture Collection 6803). A large slowing ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00046879
更新日期：1988-10-01 00:00:00
abstract：:Nonlinear optical responses of bacteriochlorophyll a (BChl a) were investigated by means of the three-pulse four-wave mixing (FWM) technique under the resonant excitation into the Q ( y ) band. The experimental results are explained by a theoretical model calculation including the Brownian oscillation mode of the solv...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-007-9266-x
更新日期：2008-02-01 00:00:00
abstract：:The thylakoid membrane protein PsbS is critical for quenching excessive excitation energy in mechanisms that involve the light-harvesting complexes of photosystem II. Liposomes of thylakoid lipids have been shown to be a very good platform to study photosynthetic membrane proteins and their interactions. In this study...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-015-0176-z
更新日期：2016-01-01 00:00:00
abstract：:Biogenesis of the photosynthetic apparatus in greening etiolated leaves of barley (Hordeum vulgare L) was investigated by an approach permitting investigation of this process under conditions that minimize differences in plastid development. Distributions of barley leaves greening for 24 h as to chlorophyll content an...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1016093127057
更新日期：2002-01-01 00:00:00
abstract：:The nucleotide sequences of the genes coding for the subunits of the Photosystem I (PS I) core, PsaA and PsaB were determined for the marine prokaryotic oxyphototrophs Prochlorococcus sp. MED4 (CCMP1378), P. marinus SS120 (CCMP1375) and Synechococcus sp. WH7803. Divergence of these sequences from those of both freshwa...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1006445810996
更新日期：2000-01-01 00:00:00
abstract：:Pulse modulated fluorescence has increasingly been used as an ecological tool to examine changes in the vertical distribution of microphytobenthic cells within the upper layers of estuarine sediments (most often using the minimum fluorescence yield F(o)) as well as to indicate the health of the community (using the ma...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/B:PRES.0000028397.86495.b5
更新日期：2004-01-01 00:00:00
abstract：:We present the wavelength dependence of homogeneous holewidths of persistent spectral holes burnt in O2-evolving Photosystem II core complexes isolated from spinach, in the temperature range 2.5-8 K. The data supports the assignment that those chlorophylls which undergo persistent spectral hole-burning are specific CP...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-004-7927-6
更新日期：2005-06-01 00:00:00
abstract：:With the discovery of targeted gene replacement, moss biology has been rapidly advancing over the last 10 years. This study demonstrates the usefulness of moss as a model organism for plant photosynthesis research. The two mosses examined in this study, Physcomitrella patens and Ceratodon purpureus, are easily culture...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-004-5577-3
更新日期：2005-01-01 00:00:00
abstract：:We have used antibodies generated against synthetic peptides to determine the topology of the 43 kD chlorophyll a binding protein (CP 43) of Photosystem II. Based on the pattern of proteolytic fragments detected (on western blots) by peptide specific antibodies, a six transmembrane span topological model, with the ami...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00019041
更新日期：1994-04-01 00:00:00
abstract：:The effect of acetate metabolism on the light energy distribution between the two photosystems, on the PS II/PS I stoichiometry and on the expression of psbA and psbB and psaA genes was investigated in the green alga, Chlamydobotrys stellata during autotrophic (CO(2)), mixotrophic (CO(2) plus acetate) and photoheterot...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1010650532693
更新日期：2000-01-01 00:00:00
abstract：:Recommendations are given for naming of genes coding for reaction center, antenna and electron transport proteins in green photosynthetic bacteria and heliobacteria. ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF02184142
更新日期：1994-07-01 00:00:00
abstract：:Oxygen may enhance CO2-saturated photosynthesis in intact leaves, which display the Warburg effect when illuminated at the current atmospheric level of CO2 and O2, of about 350 microl l(-1) and 21%, respectively. The magnitude of the stimulation depends on irradiance. The K(M)(O2) of the stimulation is 128 microM (10....
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-006-9050-3
更新日期：2006-06-01 00:00:00
