Monitoring Migration and Measuring Biomass in Benthic Biofilms: The Effects of Dark/far-red Adaptation and Vertical Migration on Fluorescence Measurements.


:Pulse modulated fluorescence has increasingly been used as an ecological tool to examine changes in the vertical distribution of microphytobenthic cells within the upper layers of estuarine sediments (most often using the minimum fluorescence yield F(o)) as well as to indicate the health of the community (using the maximum PS II quantum efficiency F(v)/F(m)). However, the practicalities of in situ measurements, often dictates that short dark adaptation periods must be used ( approximately 15 min). The use of far-red light as an alternative to dark adaptation was investigated in natural migratory microphytobenthic biofilms and artificial non-migratory biofilms. Prolonged periods of darkness ( approximately 24 h) were not adequate to achieve 'true' measurements of F(o) and F(v)/F(m), which require complete oxidation of Q(A) and full reversal of non-photochemical quenching (NPQ). In some instances, stable values were only achieved using far-red light. Prolonged exposure to dark/far-red light led to a downwards migration of cells in natural assemblages, as seen by a reduction in both F(o) and the maximum fluorescence yield (F(m)). In non-migratory biofilms, F(m) increased in the dark and far-red treatments, indicating a reversal of NPQ, whereas F(o) decreased in far-red light but increased in the dark. It is suggested that far-red light and darkness differentially affected the balance between NPQ reversal and Q(A) oxidation that lead to the measured F(o) yield. The use of far-red light as an alternative to dark adaptation is discussed and the implications of short (e.g., 15 min) dark adaptation times used in situ are discussed with reference to the vertical migration of cells within sediment biofilms.


Photosynth Res


Photosynthesis research


Consalvey M,Jesus B,Perkins RG,Brotas V,Underwood GJ,Paterson DM




Has Abstract


2004-01-01 00:00:00












  • A hydrogen-atom abstraction model for the function of YZ in photosynthetic oxygen evolution.

    abstract::Recent magnetic-resonance work on YŻ suggests that this species exhibits considerable motional flexibility in its functional site and that its phenol oxygen is not involved in a well-ordered hydrogen-bond interaction (Tang et al., submitted; Tommos et al., in press). Both of these observations are inconsistent with a ...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Hoganson CW,Lydakis-Simantiris N,Tang XS,Tommos C,Warncke K,Babcock GT,Diner BA,McCracken J,Styring S

    更新日期:1995-11-01 00:00:00

  • Thermal acclimation of photosynthesis: on the importance of adjusting our definitions and accounting for thermal acclimation of respiration.

    abstract::While interest in photosynthetic thermal acclimation has been stimulated by climate warming, comparing results across studies requires consistent terminology. We identify five types of photosynthetic adjustments in warming experiments: photosynthesis as measured at the high growth temperature, the growth temperature, ...

    journal_title:Photosynthesis research

    pub_type: 杂志文章,评审


    authors: Way DA,Yamori W

    更新日期:2014-02-01 00:00:00

  • Excited state properties of chlorophyll f in organic solvents at ambient and cryogenic temperatures.

    abstract::Chlorophyll f is a photosynthetic pigment that was discovered in 2010. In this study, we present investigations on spectral and dynamic characteristics of singlet-excited and triplet states of Chl f with the application of ultrafast time-resolved absorption and fluorescence spectroscopies. The pigment was studied at r...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Niedzwiedzki DM,Liu H,Chen M,Blankenship RE

    更新日期:2014-07-01 00:00:00

  • Routing of thylakoid lumen proteins by the chloroplast twin arginine transport pathway.

    abstract::Thylakoids are complex sub-organellar membrane systems whose role in photosynthesis makes them critical to life. Thylakoids require the coordinated expression of both nuclear- and plastid-encoded proteins to allow rapid response to changing environmental conditions. Transport of cytoplasmically synthesized proteins to...

    journal_title:Photosynthesis research

    pub_type: 杂志文章,评审


    authors: New CP,Ma Q,Dabney-Smith C

    更新日期:2018-12-01 00:00:00

  • Bacteriochlorophyllc formation and chlorosome development inChloroflexus aurantiacus.

    abstract::The dependence of chlorosome development on bacteriochlorophyll (BChl)c synthesis was studied with the phototrophic green bacteriumChloroflexus aurantiacus. By selecting defined culture conditions, three possibilities could be identified. Upon addition of 5-aminolevulinic acid, cells of resting cultures increased thei...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Foidl M,Golecki JR,Oelze J

    更新日期:1994-07-01 00:00:00

  • Photosynthesis research in the People's Republic of China.

    abstract::The first research paper on photosynthesis in China was published by T.T. Li(2) in 1929. Two photosynthesis laboratories were established in Shanghai and Beijing in the 1950s and the 1960s, respectively. A photophosphorylation 'intermediate' was discovered after the energy conversion process was separated into light a...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Kuang TY,Xu C,Li LB,Shen YK

    更新日期:2003-01-01 00:00:00

  • The conference at the airlie house in 1963.

    abstract::In many ways, the 1963 conference at Airlie House was an unusual event. The meeting convincingly demonstrated that photosynthetic research, previously reported mostly at conferences of botany and/or plant physiology, had become an independent and important field of biological research. New techniques had been develope...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Rurainski HJ

    更新日期:2004-01-01 00:00:00

  • Variation in Rubisco content and activity under variable climatic factors.

    abstract::The main objective of the present review is to provide a compilation of published data of the effects of several climatic conditions on Rubisco, particularly its activity, state of activation, and concentration, and its influence on leaf gas exchange and photosynthesis. The environmental conditions analyzed include dr...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Galmés J,Aranjuelo I,Medrano H,Flexas J

    更新日期:2013-11-01 00:00:00

  • Energy migration and exciton trapping in green plant photosynthesis.

    abstract::The possible origins of the different fluorescence decay components in green plants are discussed in terms of a random walk and Butler's bipartite model. The interaction of the excitations with the photosystem II reaction centers and, specifically, the regeneration of theses excitations by charge recombination within ...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Geacintov NE,Breton J,Knox RS

    更新日期:1986-01-01 00:00:00

  • A Brownian Dynamics computational study of the interaction of spinach plastocyanin with turnip cytochrome f: the importance of plastocyanin conformational changes.

    abstract::Brownian Dynamics (BD) computer simulations were used to study electrostatic interactions between turnip cytochrome f (cyt f) and spinach plastocyanin (PC). Three different spinach PC structures were studied: The X-ray crystal structure of Xue and coworkers [(1998) Protein Sci 7:2099-2105] and the NMR structure of Mus...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Gross EL

    更新日期:2007-11-01 00:00:00

  • The photoenzymatic cycle of NADPH: protochlorophyllide oxidoreductase in primary bean leaves (Phaseolus vulgaris) during the first days of photoperiodic growth.

    abstract::The photoenzymatic cycle of the light-dependent NADPH:protochlorophyllide oxidoreductase (LPOR) was investigated in situ during early stages of development of bean leaves under light-dark cycles (LDC). In the experimental system used in this study, prolamellar bodies developed during night periods and disappeared duri...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Schoefs B,Franck F

    更新日期:2008-04-01 00:00:00

  • Anatomy of non-uniform leaf photosynthesis.

    abstract::Since 1986, non-uniform photosynthesis over the leaf area that may be attributed to patchy stomatal closure, has been an important issue in stress physiology of photosynthesis. In this review, I first outline the gaseous environment within the intercellular spaces, because this is the most fundamental background of th...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Terashima I

    更新日期:1992-03-01 00:00:00

  • Association of small CAB-like proteins (SCPs) of Synechocystis sp. PCC 6803 with Photosystem II.

    abstract::The cyanobacterial small CAB-like proteins (SCPs) are one-helix proteins with compelling similarity to the first and third transmembrane helix of proteins belonging to the CAB family of light-harvesting complex proteins in plants. The SCP proteins are transiently expressed at high light intensity and other stress cond...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Kufryk G,Hernandez-Prieto MA,Kieselbach T,Miranda H,Vermaas W,Funk C

    更新日期:2008-02-01 00:00:00

  • Characterization of cyanobacterial cells synthesizing 10-methyl stearic acid.

    abstract::Recently, microalgae have attracted attention as sources of biomass energy. However, fatty acids from the microalgae are mainly unsaturated and show low stability in oxygenated environments, due to oxidation of the double bonds. The branched-chain fatty acid, 10-methyl stearic acid, is synthesized from oleic acid in c...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Machida S,Suzuki I

    更新日期:2019-03-01 00:00:00

  • Recollections of Thomas John Wydrzynski.

    abstract::In appreciation of his contribution to the Photosystsem II research and commemoration of the book Photosystem II: The Light-Driven Water-Plastoquinone Oxido-Reductase, co-edited with Kimiyuki Satoh, I present here some of my recollections of Thomas John Wydrzynski and by several others with whom he has associated over...

    journal_title:Photosynthesis research

    pub_type: 传,历史文章,杂志文章


    authors: Govindjee

    更新日期:2008-10-01 00:00:00

  • Trophic status of Chlamydomonas reinhardtii influences the impact of iron deficiency on photosynthesis.

    abstract::To investigate the impact of iron deficiency on bioenergetic pathways in Chlamydomonas, we compared growth rates, iron content, and photosynthetic parameters systematically in acetate versus CO(2)-grown cells. Acetate-grown cells have, predictably (2-fold) greater abundance of respiration components but also, counter-...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Terauchi AM,Peers G,Kobayashi MC,Niyogi KK,Merchant SS

    更新日期:2010-07-01 00:00:00

  • Effects of temperature and photosynthetic inhibitors on light activation of C4-phosphoenolpyruvate carboxylase.

    abstract::Phosphoenolpyruvate carboxylase from leaves of the C4 plant Setaria verticillata (L.) Beauv. is activated by light; day levels of activity are reached after 30 minutes of illumination. Photoactivation is prevented by inhibitors of photosynthetic electron flow or of photophosphorylation and by D,L-glyceraldehyde, which...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Samaras Y,Manetas Y,Gavalas NA

    更新日期:1988-06-01 00:00:00

  • The MoD-QM/MM methodology for structural refinement of photosystem II and other biological macromolecules.

    abstract::Quantum mechanics/molecular mechanics (QM/MM) hybrid methods are currently the most powerful computational tools for studies of structure/function relations and structural refinement of macrobiomolecules (e.g., proteins and nucleic acids). These methods are highly efficient, since they implement quantum chemistry tech...

    journal_title:Photosynthesis research

    pub_type: 杂志文章,评审


    authors: Sproviero EM,Newcomer MB,Gascón JA,Batista ER,Brudvig GW,Batista VS

    更新日期:2009-11-01 00:00:00

  • A new setup for in vivo fluorescence imaging of photosynthetic activity.

    abstract::Here, we describe a new imaging setup able to assess in vivo photosynthetic activity. The system specifically measures time-resolved chlorophyll fluorescence in response to light. It is composed of a fast digital camera equipped with a wide-angle lens for the analysis of samples up to 10 x 10 cm, i.e. entire plants or...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Johnson X,Vandystadt G,Bujaldon S,Wollman FA,Dubois R,Roussel P,Alric J,Béal D

    更新日期:2009-10-01 00:00:00

  • Growth, photosynthesis and photorespiration of Lemna gibba: response to variations in CO2 and O 2 concentrations and photon flux density.

    abstract::Dry weight and Relative Growth Rate of Lemna gibba were significantly increased by CO2 enrichment up to 6000 μl CO2 l(-1). This high CO2 optimum for growth is probably due to the presence of nonfunctional stomata. The response to high CO2 was less or absent following four days growth in 2% O2. The Leaf Area Ratio decr...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Andersen IH,Dons C,Nilsen S,Haugstad MK

    更新日期:1985-03-01 00:00:00

  • Characterization of chlorophyll-protein complexes isolated from two marine green algae, Bryopsis maxima and Ulva pertusa, growing in the intertidal zone.

    abstract::Three Chl-protein complexes were isolated from thylakoid membranes of Bryopsis maxima and Ulva pertusa, marine green algae that inhabit the intertidal zone of the Pacific Ocean off the eastern coast of Japan by dodecyl-beta-D-maltoside polyacrylamide gel electrophoresis. The slowest-moving fractions showed low Chl a/b...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Yamazaki JY,Kozu A,Fukunaga Y

    更新日期:2006-07-01 00:00:00

  • Light- and CO2-saturated photosynthesis: enhancement by oxygen.

    abstract::Oxygen may enhance CO2-saturated photosynthesis in intact leaves, which display the Warburg effect when illuminated at the current atmospheric level of CO2 and O2, of about 350 microl l(-1) and 21%, respectively. The magnitude of the stimulation depends on irradiance. The K(M)(O2) of the stimulation is 128 microM (10....

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Viil J,Ivanova H,Pärnik T

    更新日期:2006-06-01 00:00:00

  • On the natural selection and evolution of the aerobic phototrophic bacteria.

    abstract::This contribution gives a brief survey of the short history since the discovery of the aerobic phototrophic bacteria to focus on a general evolutionary scenario. Most of the citations are of reviews that have covered the earlier literature and to which the reader is directed at appropriate places in the following text...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Beatty JT

    更新日期:2002-01-01 00:00:00

  • Structural Basis of Redox Signaling in Photosynthesis: Structure and Function of Ferredoxin:thioredoxin Reductase and Target Enzymes.

    abstract::The role of the ferredoxin:thioredoxin system in the reversible light activation of chloroplast enzymes by thiol-disulfide interchange with thioredoxins is now well established. Recent fruitful collaboration between biochemists and structural biologists, reflected by the shared authorship of the paper, allowed to solv...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Dai S,Johansson K,Miginiac-Maslow M,Schürmann P,Eklund H

    更新日期:2004-01-01 00:00:00

  • André Tridon Jagendorf (1926-2017): a personal tribute.

    abstract::We present here our tribute to an outstanding unique scientist, a pioneer of plant biochemistry, best known for his seminal ground-breaking discoveries related to electron and proton transport, and their intimate relation to ATP synthesis in chloroplasts. His dedication and in-depth understanding of science was matche...

    journal_title:Photosynthesis research

    pub_type: 传,历史文章,杂志文章


    authors: Govindjee

    更新日期:2017-06-01 00:00:00

  • Preparation and functional characterization of thylakoids from Arabidopsis thaliana.

    abstract::A protocol for the isolation of functional thylakoids from Arabidopsis thaliana leaves was developed. The critical factor in obtaining active, coupled and stable preparation is the inclusion of EDTA and EGTA in the grinding buffer. Preparations were characterized with respect to the whole or partial electron transport...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Casazza AP,Tarantino D,Soave C

    更新日期:2001-01-01 00:00:00

  • Artificial increase of the light-harvesting ability of photosynthetic units in isolated chloroplasts.

    abstract::A synthetic fluorochromous lipid, rhodaminyl triglyceride (rhodaminyl TG), was intercalated into isolated thylakoid membranes of chloroplasts up to 30 molecules per 100 molecules of chlorophyll. As a result of fluorochrome presence, an absorption band appeared in a yellow-green spectrum region, its intensity being com...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Sorokin EM,Bobylyev GS,Molotkovsky YG

    更新日期:1990-11-01 00:00:00

  • Photosynthetic energy storage efficiency in Chlamydomonas reinhardtii, based on microsecond photoacoustics.

    abstract::Using a novel, pulsed micro-second time-resolved photoacoustic (PA) instrument, we measured thermal dissipation and energy storage (ES) in the intact cells of wild type (WT) Chlamydomonas reinhardtii, and mutants lacking either PSI or PSII reaction centers (RCs). On this time scale, the kinetic contributions of the th...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Yan C,Schofield O,Dubinsky Z,Mauzerall D,Falkowski PG,Gorbunov MY

    更新日期:2011-09-01 00:00:00

  • The relationship of intercompartmental excitation transfer rate constants to those of an underlying physical model.

    abstract::In studies on photosynthetic systems it is common practice to interpret the results of time-resolved fluorescence experiments on the basis of compartmental, or target, models. Each compartment represents a group of molecules with similar fluorescence characteristics. In cases of practical interest, the members of each...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Holcomb CT,Knox RS

    更新日期:1996-11-01 00:00:00

  • NMR analysis of plant nitrogen metabolism.

    abstract::The analysis of primary and secondary nitrogen metabolism in plants by nuclear magnetic resonance (NMR) spectroscopy is comprehensively reviewed. NMR is a versatile analytical tool, and the combined use of (1)H, (2)H, (13)C, (14)N and (15)N NMR allows detailed investigation of the acquisition, assimilation and metabol...

    journal_title:Photosynthesis research

    pub_type: 杂志文章,评审


    authors: Mesnard F,Ratcliffe RG

    更新日期:2005-01-01 00:00:00