Abstract：
:Phosphoenolpyruvate carboxylase from leaves of the C4 plant Setaria verticillata (L.) Beauv. is activated by light; day levels of activity are reached after 30 minutes of illumination. Photoactivation is prevented by inhibitors of photosynthetic electron flow or of photophosphorylation and by D,L-glyceraldehyde, which inhibits the reductive pentose phosphate pathway.Although the extractable activity in the dark is not affected by temperature the photoactivation is prevented when both illumination and extraction are done under low temperature (5 C). High temperature (30 C) during either illumination or extraction is needed for activation. Once the enzyme is photoactivated at 30 C, a transfer of the leaves to 5 C does not abolish the extra activity.The results suggest that both unimpaired electron flow and photophosphorylation are prerequisites for the activation of phosphoenolpyruvate carboxylase. Low temperature apparently suppresses either the transport to the cytoplasm of a photosynthetic intermediate or the activating reaction itself. The inclusion of phosphoenolpyruvate in the extraction medium increases the night activity.On the basis of the available information, it is suggested that phosphoenolpyruvate could be the activator in vivo. In that case, the activation of phosphoenolpyruvate carboxylase would depend on internal CO2 level and prior photoactivation of both pyruvate, orthophosphate, dikinase and NADP malate dehydrogenase.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Samaras Y,Manetas Y,Gavalas NAdoi
10.1007/BF00028842subject
Has Abstractpub_date
1988-06-01 00:00:00pages
233-42issue
3eissn
0166-8595issn
1573-5079journal_volume
16pub_type
杂志文章abstract：:The analysis of primary and secondary nitrogen metabolism in plants by nuclear magnetic resonance (NMR) spectroscopy is comprehensively reviewed. NMR is a versatile analytical tool, and the combined use of (1)H, (2)H, (13)C, (14)N and (15)N NMR allows detailed investigation of the acquisition, assimilation and metabol...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-004-2081-8
更新日期：2005-01-01 00:00:00
abstract：:The possible origins of the different fluorescence decay components in green plants are discussed in terms of a random walk and Butler's bipartite model. The interaction of the excitations with the photosystem II reaction centers and, specifically, the regeneration of theses excitations by charge recombination within ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00118288
更新日期：1986-01-01 00:00:00
abstract：:The light-harvesting complex (LHC) of higher plants isolated using Triton X-100 has been studied during its transformation into a monomeric form known as CPII. The change was accomplished by gradually increasing the concentration of the detergent, sodium dodecyl sulfate (SDS). Changes in the red spectral region of the...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00029727
更新日期：1986-01-01 00:00:00
abstract：:Fourier transform infrared (FTIR) spectroscopy probes the vibrational properties of amino acids and cofactors, which are sensitive to minute structural changes. The lack of specificity of this technique, on the one hand, permits us to probe directly the vibrational properties of almost all the cofactors, amino acid si...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-009-9439-x
更新日期：2009-08-01 00:00:00
abstract：:This contribution gives a brief survey of the short history since the discovery of the aerobic phototrophic bacteria to focus on a general evolutionary scenario. Most of the citations are of reviews that have covered the earlier literature and to which the reader is directed at appropriate places in the following text...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1020493518379
更新日期：2002-01-01 00:00:00
abstract：:Regulation of photosynthetic electron transport at different levels of structural and functional organization of photosynthetic apparatus provides efficient performance of oxygenic photosynthesis in plants. This review begins with a brief overview of the chloroplast electron transport chain. Then two noninvasive bioph...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-015-0094-0
更新日期：2015-08-01 00:00:00
abstract：:I provide here a personal perspective on (i) the awards that were given to young investigators at the 2008 and 2009 Gordon Research Conferences on Biochemistry/Biophysics of Photosynthesis; and (ii) the ambiance at these conferences through some photographs, particularly of the 2009 conference. ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-009-9479-2
更新日期：2009-10-01 00:00:00
abstract：:It has been known for some time that bicarbonate reverses the inhibition, by formate under HCO3 (-)-depletion conditions, of electron transport in thylakoid membranes. It has been shown that the major effect is on the electron acceptor side of photosystem II, at the site of plastoquinone reduction. After presenting a ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00114571
更新日期：1988-01-01 00:00:00
abstract：:The binding characteristics and the inhibitory power of atrazine and DCMU towards uncoupled electron flow activity were studied in acyl lipid-depleted thylakoid membranes from atrazine-susceptible and-resistant biotypes of Solanum nigrum L. For this purpose, phospholipase A2 from Vipera russelli and the lipase from Rh...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00049537
更新日期：1992-01-01 00:00:00
abstract：:A detailed analysis of the periplasmic electron carriers of the photosynthetic bacterium Ectothiorhodospira sp. has been performed. Two low mid-point redox potential electron carriers, cytochrome c' and cytochrome c, are detected. A high potential iron-sulfur protein is the only high mid-point redox potential electron...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1006486307473
更新日期：2000-01-01 00:00:00
abstract：:The current status of research on the structure, regulation, mechanism and importance of Rubisco activase is reviewed. The activase is now recognized to be a member of the AAA(+) family, whose members participate in macromolecular complexes that perform diverse chaperone-like functions. The conserved nucleotide-bindin...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1022458108678
更新日期：2003-01-01 00:00:00
abstract：:There is diverse, yet controversial fossil evidence for the existence of photosynthesis 3500 million years ago. Among the most persuasive evidence is the stromatolites described from low grade metasedimentary rocks in Western Australia and South Africa. Based on the understanding of the paleobiology of stromatolites a...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00039172
更新日期：1992-08-01 00:00:00
abstract：:An immunological method for quantitative determination of photosynthetic fructose-1,6-bisphosphatase in crude extracts of leaves is proposed. It is based on the ELISA technique, and offers two modifications. A non-competitive technique has a higher sensitivity and is the right option for samples of low fructose-1,6-bi...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00032710
更新日期：1987-01-01 00:00:00
abstract：:Taro and cocoyam were grown outdoors in either full sun or under 40% shade. Leaves were tagged as they emerged and the effect of leaf age on net CO2 assimilation rate (A) was determined. The effects of shading on A, transpiration (E), stomatal conductance for CO2 (gc) and H2O (gs), and water use efficiency (WUE) were ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00055064
更新日期：1987-01-01 00:00:00
abstract：:A recent publication (Esteban in New Phytol 217:341-342, 2018) describes how the use and citation of the assay of chlorophylls a and b extracted in aqueous 80% acetone by Arnon (Plant Physiol 2:1-15, 1949) is increasing, even in journals with high impact factors. This is a very disconcerting situation: the assay is ou...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-018-0579-8
更新日期：2019-05-01 00:00:00
abstract：:The crystallization of a given protein is a hard task being even more complicated when the protein shows a hydrophobic behavior. In the case of photosynthetic proteins, the difficulty of the experiments increased due to the high light sensitivity. Aqueous solutions of photosystem II core complex (OEC PSII) of Pisum sa...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-007-9131-y
更新日期：2006-12-01 00:00:00
abstract：:The cyanobacterial small CAB-like proteins (SCPs) are one-helix proteins with compelling similarity to the first and third transmembrane helix of proteins belonging to the CAB family of light-harvesting complex proteins in plants. The SCP proteins are transiently expressed at high light intensity and other stress cond...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-007-9244-3
更新日期：2008-02-01 00:00:00
abstract：:A chloroplast preparation was extracted from squash (Cucurbita pepo (L.) var. Senator). Enrichment of intact chloroplasts was achieved by continuous free-flow electrophoresis. The addition of monoterpenes, detergent and free fatty acids changed the elecrophoretic separation pattern characteristically. Monoterpene-depe...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00054124
更新日期：1991-06-01 00:00:00
abstract：:Chloroplast NADP-dependent malate dehydrogenase (NADP-MDH, EC 1.1.1.82) is inactive in the dark and activated in the light via a reduction of specific disulfides by thiol-disulfide interchange with thioredoxin, reduced by the photosynthetic electron transfer. Compared to the constitutively active NAD-dependent forms, ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1016099228450
更新日期：2002-01-01 00:00:00
abstract：:Water vapor was added differentially to the gas streams entering the cells of three makes of differential infrared carbon dioxide analysers. Analyser deflections were compared with those expected from dilution of the carbon dioxide by the additional gas. Tests were made at 0, 365, and 730 cm(3) m(-3) concentrations of...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00054104
更新日期：1985-12-01 00:00:00
abstract：:Photosynthesis and carbohydrate metabolism of higher plants need to be tightly regulated to prevent tissue damage during environmental changes. The intracellular position of chloroplasts changes due to a changing light regime. Chloroplast avoidance and accumulation response under high and low light, respectively, are ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-020-00795-y
更新日期：2021-01-01 00:00:00
abstract：:To determine the fluorescence properties of cyanobacterial Photosystem I (PS I) in relatively intact systems, fluorescence emission from 20 to 295 K and polarization at 77 K have been measured from phycobilisomes-less thylakoids of Synechocystis sp. PCC 6803 and a mutant strain lacking Photosystem II (PS II). At 295 K...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00028785
更新日期：1992-02-01 00:00:00
abstract：:Recent magnetic-resonance work on YŻ suggests that this species exhibits considerable motional flexibility in its functional site and that its phenol oxygen is not involved in a well-ordered hydrogen-bond interaction (Tang et al., submitted; Tommos et al., in press). Both of these observations are inconsistent with a ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00020428
更新日期：1995-11-01 00:00:00
abstract：:The engagement of different photoprotective mechanisms in the cells of the carotenogenic astaxanthin-accumulating chlorophyte Haematococcus pluvialis (i) under favorable conditions, (ii) in the course of stress-induced haematocyst formation and (iii) during recovery from the stress was studied. To this end, we followe...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-016-0246-x
更新日期：2016-06-01 00:00:00
abstract：:Flash-induced absorbance measurements at 830 nm on both nanosecond and microsecond timescales have been used to characterise the effect of ultraviolet light on Photosystem II core particles. A combination of UV-A and UV-B, closely simulating the spectrum of sunlight below 350 nm, was found to have a primary effect on ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00029424
更新日期：1996-07-01 00:00:00
abstract：:Intra- and inter-specific variation in CO2 assimilation rate (A) in Triticum spp. is well documented for reproductive growth stages. Research was conducted to characterize early vegetative photosynthetic variation in a diverse set of cultivated hexaploid wheat (T. aestivum L.) germplasm and in wild tetraploid (T. dico...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00034120
更新日期：1989-05-01 00:00:00
abstract：:This article gives an historical overview of our group's research on various metabolite translocators of chloroplasts, such as the translocators for phosphorylated intermediates of the Calvin-Benson cycle and of glycolysis, of ADP and ATP, of dicarboxylates, of pyruvate and of hexoses; how it began and where it led to...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1020475803784
更新日期：2002-01-01 00:00:00
abstract：:Green sulfur bacteria and heliobacteria are strictly anaerobic phototrophs that have homodimeric type 1 reaction center complexes. Within these complexes, highly reducing substances are produced through an initial charge separation followed by electron transfer reactions driven by light energy absorption. In order to ...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-009-9521-4
更新日期：2010-06-01 00:00:00
abstract：:We present the wavelength dependence of homogeneous holewidths of persistent spectral holes burnt in O2-evolving Photosystem II core complexes isolated from spinach, in the temperature range 2.5-8 K. The data supports the assignment that those chlorophylls which undergo persistent spectral hole-burning are specific CP...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-004-7927-6
更新日期：2005-06-01 00:00:00
abstract：:The author gives an autobiographical sketch of his path to chlorophyll research, and describes some results. The discussion is largely focused on long wavelength forms of chlorophyll and how they might be generated by self-assembly. Dimers or oligomers, (Chl)n, result from coordination interactions between the central...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00033128
更新日期：1990-12-01 00:00:00
