The effects of ultraviolet irradiation on P680(+) reduction in PS II core complexes measured for individual S-states and during repetitive cycling of the oxygen-evolving complex.

Abstract:

:Flash-induced absorbance measurements at 830 nm on both nanosecond and microsecond timescales have been used to characterise the effect of ultraviolet light on Photosystem II core particles. A combination of UV-A and UV-B, closely simulating the spectrum of sunlight below 350 nm, was found to have a primary effect on the donor side of P680. Repetitive measurements indicated reductions in the nanosecond components of the absorbance decay with a concomitant appearance and increase in the amplitude of a component with a 10 μs time constant attributed to slow reduction of P680(+) by Tyrz when the function of the oxygen evolving complex is inhibited. Single-flash measurements show that the nanosecond components have amplitudes which vary with S-state. Increasing UV irradiation inhibited the amplitude of these components without changing their S-state dependence. In addition, UV irradiation resulted in a reduction in the total amplitude, with no change in the proportion of the 10 μs contribution.

journal_name

Photosynth Res

journal_title

Photosynthesis research

authors

Post A,Lukins PB,Walker PJ,Larkum AW

doi

10.1007/BF00029424

subject

Has Abstract

pub_date

1996-07-01 00:00:00

pages

21-7

issue

1

eissn

0166-8595

issn

1573-5079

journal_volume

49

pub_type

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