Abstract：
:The Photosystem I reaction centre protein CP1, isolated from barley using polyacrylamide gel electrophoresis showed an EPR (Electron Paramgnetic Resonance) spectrum with the polarisation pattern AEEAAE, typical of the primary donor triplet state (3)P700, created via radical pair formation and recombination. (3)P700 could also be detected by Fluorescence Detected Magnetic Resonance (FDMR) at λf > 700 nm even in the presence of a large number of chlorophyll antennae. Its zero field splitting parameters, D=282.5×10(-4) cm(-1) and E=38.5×10(-4) cm(-1), were independent of the detection wavelength, and agreed with ADMR (Absorption Detected Magnetic Resonance) and EPR values. The signs of the (3)P700 D+E and D-E transitions were positive (increase in fluorescence intensity on applying a resonance microwave field). In contrast, in the emission band 685 < λf < 700 nm FDMR spectra with negative D+E and D-E transitions were detected, and the D value was wavelength-dependent. These FDMR results support an excitation energy transfer model for CP1, derived from time-resolved fluorescence studies, in which two chlorophyll antenna forms are distinguished, with fluorescence at 685 < λf < 700 nm (inner core antennae, F690), and λf > 700 nm (low energy antenna sites, F720), in addition to the P700. The FDMR spectrum in F690 emission can be interpreted as that of (3)P700, observed via reverse singlet excitation energy transfer and added to the FDMR spectrum of the antenna triplet states generated via intramolecular intersystem crossing. This would indicate that reversible energy transfer between F690 and P700 occurs even at 4.2 K.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Searle GF,Schaafsma TJdoi
10.1007/BF00034795subject
Has Abstractpub_date
1992-06-01 00:00:00pages
193-206issue
3eissn
0166-8595issn
1573-5079journal_volume
32pub_type
杂志文章abstract：:Fluorescence and electron paramagnetic resonance measurements were used to study selenium influence on photosystem activity in rape seedlings affected by Cd stress. Water cultures containing Hoagland nutrients were supplemented with 400 microM of CdCl(2), 2 microM of Na(2)SeO(4) and a mixture of both CdCl(2) and Na(2)...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-010-9551-y
更新日期：2010-07-01 00:00:00
abstract：:A correlated influence of cation concentration and excitation energy level on PS II Abbreviations: AMPD = 2-amino-2-methyl-1,3-propanediol; DCIP = 2-6 dichlorophenol indophenol; DCIPH2 = DCIP in reduced state; DCIPr = rate of DCIP-reduction; DCMU = 3-(3,4 dichlorophenyl)1-1 dimethyl-urea; Fv = variable fluorescence; F...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00018228
更新日期：1980-06-01 00:00:00
abstract：:The availability of a complete genome database for the cyanobacterium Synechocystis sp. PCC6803 (glucose-tolerant strain) has raised expectations that this organism would become a reference strain for work aimed at understanding the CO(2)-concentrating mechanism (CCM) in cyanobacteria. However, the amount of physiolog...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1025850230977
更新日期：2003-01-01 00:00:00
abstract：:We pay tribute to the life and work of Margareta Ryberg (1946-2012). She was an expert on the different forms of protochlorophyll(ide), their protein partners, and their transformations in angiosperms; on the structural aspects, and the nature of prolamellar bodies, as well as on the localization of light-dependent NA...
journal_title：Photosynthesis research
pub_type： 传,历史文章,杂志文章
doi：10.1007/s11120-012-9752-7
更新日期：2012-09-01 00:00:00
abstract：:Previous investigations have shown that increased source strength as a result of elevated CO(2) can alter the timing of the phases of change that occur in photosynthetic rates during dicot leaf ontogeny [Miller et al. (1997) Plant Physiol 115: 1195-1200]. To evaluate the converse situation of decreased source strength...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1006367719639
更新日期：2000-01-01 00:00:00
abstract：:The effects of root applications of kinetin, gibberellic acid (GA3) and indoleacetic acid (IAA) on photosynthesis was measured using an open infrared CO2 gas-exchange system. There was a 30-35% increase in the photosynthetic rates (mg CO2/dm(2)/hr) of attached leaves within 8 hr following root treatment with 0.47 μM k...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00030048
更新日期：1982-01-01 00:00:00
abstract：:A pigment system containing carotenoids and oxidised reaction centre pigments is present in chromatophores of Rhodospirillum rubrum and this pigment system may cause fluorescence quenching when a still unidentified chromatophore component is in its oxidised state. Besides by its action spectrum, this pigment system is...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00018376
更新日期：1984-03-01 00:00:00
abstract：:To investigate the impact of iron deficiency on bioenergetic pathways in Chlamydomonas, we compared growth rates, iron content, and photosynthetic parameters systematically in acetate versus CO(2)-grown cells. Acetate-grown cells have, predictably (2-fold) greater abundance of respiration components but also, counter-...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-010-9562-8
更新日期：2010-07-01 00:00:00
abstract：:Chlorosomes of green photosynthetic bacteria are the most amazing example of long-range ordered natural light-harvesting antennae. Chlorosomes are the largest among all known photosynthetic light-harvesting structures (~ 104-105 pigments in the aggregated state). The chlorosomal bacteriochlorophyll (BChl) c/d/e molecu...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-019-00707-9
更新日期：2020-12-01 00:00:00
abstract：:Using 77 K chlorophyll a (Chl a) fluorescence spectra in vivo, the development was studied of Photosystems II (PS II) and I (PS I) during greening of barley under intermittent light followed by continuous light at low (LI, 50 mumol m(-2) s(-1)) and high (HI, 1000 mumol m(-2) s(-1)) irradiances. The greening at HI inte...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1020943107318
更新日期：2002-01-01 00:00:00
abstract：:Almost 1 year has passed since the death of Wolfgang Haehnel on 13.5.2013, after a long fight against cancer. With his death, the photosynthesis community has lost an excellent scientist, a critical peer, and a stimulating sparring partner in discussions: I also lost a good friend. ...
journal_title：Photosynthesis research
pub_type： 传,历史文章
doi：10.1007/s11120-014-9999-2
更新日期：2014-06-01 00:00:00
abstract：:Oxygenic photosynthesis requires chlorophyll (Chl) for the absorption of light energy, and charge separation in the reaction center of photosystem I and II, to feed electrons into the photosynthetic electron transfer chain. Chl is bound to different Chl-binding proteins assembled in the core complexes of the two photo...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-015-0154-5
更新日期：2015-12-01 00:00:00
abstract：:Around 1960 experiments of Arnold and Clayton, Chance and Nishimura and Calvin and coworkers demonstrated that the primary photosynthetic electron transfer processes are not abolished by cooling to cryogenic temperatures. After a brief historical introduction, this review discusses some aspects of electron transfer in...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00041000
更新日期：1996-05-01 00:00:00
abstract：:The interaction of PsaC with the core heterodimer of Photosystem 1 was studied in wild type Synechocystis sp. PCC 6803 and the site-directed mutant R561E of PsaB. The mutant reaction center was much less stable in urea and the functional reconstitution of the mutant core using PsaC was impaired. However, the extent of...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00018261
更新日期：1994-12-01 00:00:00
abstract：:After a brief prologue on Otto Kandler's life, we describe briefly his pioneering work on photosynthesis (photophosphorylation and the carbon cycle) and his key participation in the discovery of the concept of three forms of life (Archaea, Prokarya, and Eukarya). With Otto Kandler's passing, both the international pho...
journal_title：Photosynthesis research
pub_type： 传,历史文章,杂志文章
doi：10.1007/s11120-018-0530-z
更新日期：2018-09-01 00:00:00
abstract：:The distribution of net photosynthetic activity of leaves was measured in a mixed grass (Setaria sphacelata var. sericea)-legume (Desmodium intortum) pasture stand using a method based on concurrent measurement of the rate of CO2 exchange, and (14)CO2 dosing followed by rapid harvesting according to height strata. Com...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00052375
更新日期：1983-06-01 00:00:00
abstract：:The role of photosynthetic pigments in the development of separation methods in biochemistry during the period 1900-1980 is described beginning with M. Tswett who introduced separation of chlorophylls and carotenoids on columns and coined the term chromatography in 1906. In Uppsala, T. Svedberg developed the ultracent...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1024944606930
更新日期：2003-01-01 00:00:00
abstract：:We present a theoretical study of excitation dynamics in the chlorosome antenna complex of green photosynthetic bacteria based on a recently proposed model for the molecular assembly. Our model for the excitation energy transfer (EET) throughout the antenna combines a stochastic time propagation of the excitonic wave ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-014-9978-7
更新日期：2014-06-01 00:00:00
abstract：:Oxygen evolving photosystem II particles were exposed to 100 and 250 W m(-2) white light at 20°C under aerobic, anaerobic and strongly reducing (presence of dithionite) conditions. Three types of photoinactivation processes with different kinetics could be distinguished: (1) The fast process which occurs under strongl...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00030061
更新日期：1990-01-01 00:00:00
abstract：:Plants are exposed to a temporally and spatially heterogeneous environment, and photosynthesis is well adapted to these fluctuations. Understanding of the complex, non-linear dynamics of photosynthesis in fluctuating light requires novel-modeling approaches that involve not only the primary light and dark biochemical ...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-007-9178-9
更新日期：2007-07-01 00:00:00
abstract：:An immunological method for quantitative determination of photosynthetic fructose-1,6-bisphosphatase in crude extracts of leaves is proposed. It is based on the ELISA technique, and offers two modifications. A non-competitive technique has a higher sensitivity and is the right option for samples of low fructose-1,6-bi...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00032710
更新日期：1987-01-01 00:00:00
abstract：:The crystallization of a given protein is a hard task being even more complicated when the protein shows a hydrophobic behavior. In the case of photosynthetic proteins, the difficulty of the experiments increased due to the high light sensitivity. Aqueous solutions of photosystem II core complex (OEC PSII) of Pisum sa...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-007-9131-y
更新日期：2006-12-01 00:00:00
abstract：:The authors present a study of the fluorescence and absorbance transients occurring in whole cells of purple nonsulfur bacterium Rhodobacter sphaeroides on the millisecond timescale under pulsed actinic illumination. The fluorescence induction curve is interpreted in terms of combination of effects of redox changes in...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-010-9569-1
更新日期：2010-08-01 00:00:00
abstract：:We have previously investigated the response mechanisms of photosystem II complexes from spinach to strong UV and visible irradiations (Wei et al J Photochem Photobiol B 104:118-125, 2011). In this work, we extend our study to the effects of strong light on the unusual cyanobacterium Acaryochloris marina, which is abl...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-013-9946-7
更新日期：2013-11-01 00:00:00
abstract：:Biohybrid light-harvesting architectures can be constructed that employ native-like bacterial photosynthetic antenna peptides as a scaffold to which synthetic chromophores are attached to augment overall spectral coverage. Synthetic bacteriochlorins are attractive to enhance capture of solar radiation in the photon-ri...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-014-0021-9
更新日期：2014-11-01 00:00:00
abstract：:There is diverse, yet controversial fossil evidence for the existence of photosynthesis 3500 million years ago. Among the most persuasive evidence is the stromatolites described from low grade metasedimentary rocks in Western Australia and South Africa. Based on the understanding of the paleobiology of stromatolites a...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00039172
更新日期：1992-08-01 00:00:00
abstract：:The dependence of chlorosome development on bacteriochlorophyll (BChl)c synthesis was studied with the phototrophic green bacteriumChloroflexus aurantiacus. By selecting defined culture conditions, three possibilities could be identified. Upon addition of 5-aminolevulinic acid, cells of resting cultures increased thei...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF02184154
更新日期：1994-07-01 00:00:00
abstract：:The coupling factor CF1 is a catalytic part of chloroplast ATP synthase which is exposed to stroma whose viscosity is many-fold higher than that of reaction mixtures commonly used to measure kinetics of CF1-catalyzed ATP hydrolysis. This study is focused on the effect of medium viscosity modulated by sucrose or bovine...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-015-0213-y
更新日期：2016-05-01 00:00:00
abstract：:Exposure of the red alga Porphyra perforata or leaves of Phytolacca americana and Echinodorus sp. to white light equivalent to full sunlight for short periods induced large decreases of variable fluorescence measured at 695 nm at 77K. This change was not produced by photoinhibition but rather appeared to result from a...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00118298
更新日期：1986-01-01 00:00:00
abstract：:The post-translational modification consisting in the formation/reduction of disulfide bonds has been the subject of intense research in plants since the discovery in the 1970s that many chloroplastic enzymes are regulated by light through dithiol-disulfide exchange reactions catalyzed by oxidoreductases called thiore...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-009-9501-8
更新日期：2010-04-01 00:00:00
