Abstract：
:The coupling factor CF1 is a catalytic part of chloroplast ATP synthase which is exposed to stroma whose viscosity is many-fold higher than that of reaction mixtures commonly used to measure kinetics of CF1-catalyzed ATP hydrolysis. This study is focused on the effect of medium viscosity modulated by sucrose or bovine serum albumin (BSA) on kinetics of Ca(2+)- and Mg(2+)-dependent ATP hydrolysis by CF1. These agents were shown to reduce the maximal rate of Ca(2+)-dependent ATPase without changing the apparent Michaelis constant (К m), thus supporting the hypothesis on viscosity dependence of CF1 activity. For the sulfite- and ethanol-stimulated Mg(2+)-dependent reaction, the presence of sucrose increased К m without changing the maximal rate that is many-fold as high as that of Ca(2+)-dependent hydrolysis. The hydrolysis reaction was shown to be stimulated by low concentrations of BSA and inhibited by its higher concentrations, with the increasing maximal reaction rate estimated by extrapolation. Sucrose- or BSA-induced inhibition of the Mg(2+)-dependent ATPase reaction is believed to result from diffusion-caused deceleration, while its BSA-induced stimulation is probably caused by optimization of the enzyme structure. Molecular mechanisms of the inhibitory effect of viscosity are discussed. Taking into account high protein concentrations in the chloroplast stroma, it was suggested that kinetic parameters of ATP hydrolysis, and probably those of ATP synthesis in vivo as well, must be quite different from measurements taken at a viscosity level close to that of water.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Malyan ANdoi
10.1007/s11120-015-0213-ysubject
Has Abstractpub_date
2016-05-01 00:00:00pages
163-8issue
2eissn
0166-8595issn
1573-5079pii
10.1007/s11120-015-0213-yjournal_volume
128pub_type
杂志文章abstract：:In appreciation of his contribution to the Photosystsem II research and commemoration of the book Photosystem II: The Light-Driven Water-Plastoquinone Oxido-Reductase, co-edited with Kimiyuki Satoh, I present here some of my recollections of Thomas John Wydrzynski and by several others with whom he has associated over...
journal_title：Photosynthesis research
pub_type： 传,历史文章,杂志文章
doi：10.1007/s11120-008-9341-y
更新日期：2008-10-01 00:00:00
abstract：:Photoacclimation by strains of Haslea "blue" diatom species H. ostrearia and H. silbo sp. nov. ined. was investigated with rapid light curves and induction-recovery curves using fast repetition rate fluorescence. Cultures were grown to exponential phase under 50 µmol m-2 s-1 photosynthetic available radiation (PAR) an...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-018-0508-x
更新日期：2018-09-01 00:00:00
abstract：:MOA-stilbene is known to be a specific inhibitor of the Qo site of mammalian cytochrome bc 1 complex. We show that it also binds to the chloroplast cytochrome bf complex. Binding to the reduced enzyme induces a red-shift of the Soret and visible absorption bands of the haems b. Steady state and single turnover experim...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00029819
更新日期：1992-12-01 00:00:00
abstract：:A fluorescence video imaging system utilizing relatively inexpensive commercial components is described. The instrument utilizes a black and white CCD video camera detector, a commercial video imaging board and a IBM-AT compatible computer. The color output of the imaging board greatly aids in the users ability to vis...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00048977
更新日期：1990-10-01 00:00:00
abstract：:The thylakoid membrane protein PsbS is critical for quenching excessive excitation energy in mechanisms that involve the light-harvesting complexes of photosystem II. Liposomes of thylakoid lipids have been shown to be a very good platform to study photosynthetic membrane proteins and their interactions. In this study...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-015-0176-z
更新日期：2016-01-01 00:00:00
abstract：:Two-component regulatory systems that respond to changes in redox potential have recently been discovered in bacteria. 'Redox sensors' are defined as electron carriers which initiate control of gene expression upon oxidation or reduction. 'Redox response regulators' are defined as DNA-binding proteins which modify gen...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00016274
更新日期：1993-05-01 00:00:00
abstract：:A short list of the twentieth century's dominant figures in photosynthesis would unquestionably include Otto Warburg. One of his many discoveries, the 'bicarbonate effect' remains a lasting puzzle to his heirs in the field. Recent developments in this area of research have renewed interest and call for a re-examinatio...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1020447030191
更新日期：2002-01-01 00:00:00
abstract：:A rapid procedure has been developed for the isolation of the photosystem two reaction centre complex (PS II RC) from a double mutant of Chlamydomonas reinhardtii, F54-14, which lacks the Photosystem one complex and the chloroplast ATPase. Thylakoid membranes are solubilised with 1.5% (w/v) Triton X-100 and the PS II ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00042974
更新日期：1995-02-01 00:00:00
abstract：:The dependence on temperature of tryptophan fluorescence lifetime in trimeric photosystem I (PSI) complexes from cyanobacteria Synechocystis sp. PCC 6803 during the heating of pre-frozen to - 180 °C in the dark or in the light-activated preparations has been studied. Fluorescence lifetime in samples frozen in the ligh...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-018-0595-8
更新日期：2019-03-01 00:00:00
abstract：:A combination of inhibitor and (15)N studies were used to investigate the photorespiratory nitrogen cycle in maize, a C4 plant. Inhibitors used included isonicotinyl hydrazide which blocks the conversion of glycine to serine, methionine sulfoximine an inhibitor of GS and azaserine an inhibitor of GOGAT. Results from l...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00041796
更新日期：1983-01-01 00:00:00
abstract：:The previous work in our lab showed that the spinach chloroplast ATP synthase epsilon mutant with 3 amino acid residues deleted from the N-terminus had much lower ability to inhibit ATP hydrolysis and block proton leakage in comparison to a mutant with 1 or 2 residues deleted from the N-terminus. The present study aim...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-004-6554-6
更新日期：2005-01-01 00:00:00
abstract：:The 3- and 7-formyl groups of chlorophyll-d (Chl-d) and bacteriochlorophyll-e (BChl-e), respectively, were regioselectively labeled with an isotopically stable oxygen-18 (¹⁸O) atom to give 3¹-¹⁸O-labeled Chl-d and 7¹-¹⁸O-labeled BChl-e (ca. 90% ¹⁸O) by exchanging the carbonyl oxygen atoms in the presence of acidic H₂ ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-010-9590-4
更新日期：2010-09-01 00:00:00
abstract：:Relationships between induced high leaf intercellular CO₂ concentrations, leaf K⁺ and NO₃⁻ ion movement and early fruit formation under macronutrient limitation are not well understood. We examined the effects and interactions of reduced K/N input treatments on leaf intercellular CO₂, photosynthesis rate, carboxylatio...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-013-9832-3
更新日期：2013-07-01 00:00:00
abstract：:Light-harvesting Chl a/b protein complexes were isolated from the higher plant Sinapis alba, the green alga Chlorella fusca, and the prasinophycean alga Mantoniella squamata by mild gel electrophoresis. The energy transfer from chlorophyll b and the accessory xanthophyll was measured by means of fluoresence spectrosco...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00035234
更新日期：1987-01-01 00:00:00
abstract：:Significance of molecular crowding in grana thylakoids of higher plants on photosystem II function was studied by 'titrating' the naturally high protein density by fusing unilamellar liposomes of the native lipid mixture with isolated grana membranes (BBY). The incorporation of lipids was monitored by equilibrium dens...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-007-9253-2
更新日期：2008-02-01 00:00:00
abstract：:Roderick K. Clayton passed away on October 23, 2011, at the age of 89, shortly after the plan for this dedicatory issue of Photosynthesis Research had been hatched. I had just written a lengthy letter to him to re-establish contact after a hiatus of 2 or 3 years, and to suggest that I visit him to talk about his life....
journal_title：Photosynthesis research
pub_type： 传,历史文章,杂志文章
doi：10.1007/s11120-013-9948-5
更新日期：2014-05-01 00:00:00
abstract：:Cross-reconstitution of the extrinsic proteins and Photosystem II (PS II) from a green alga, Chlamydomonas reinhardtii, and a higher plant,Spinacia oleracea, was performed to clarify the differences of binding properties of the extrinsic proteins between these two species of organisms. (1) Chlamydomonas PsbP and PsbQ ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-004-7760-y
更新日期：2005-06-01 00:00:00
abstract：:The Mg-dechelating activity of extracts of Chenopodium album (goosefoot) was investigated using an artificial substrate, chlorophyllin a. The activity was measured spectrophotometrically by the formation of a reaction product, pheophorbin a (Mg-free chlorin), after release of the central Mg. The Mg-releasing protein w...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1020915812770
更新日期：2002-01-01 00:00:00
abstract：:Soybean [Glycine max (L.) Merr. cv. Williams 82 and A3127] plants were grown in the field under long-term soil moisture deficit and irrigation to determine the effects of severe drought stress on the photosynthetic capacity of soybean leaves. Afternoon leaf water potentials, stomatal conductances, intercellular CO2 co...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00051735
更新日期：1990-07-01 00:00:00
abstract：:This review focuses on our recent efforts in synthetic ruthenium-tyrosine-manganese chemistry mimicking the donor side reactions of Photosystem II. Tyrosine and tryptophan residues were linked to ruthenium photosensitizers, which resulted in model complexes for proton-coupled electron transfer from amino acids. A new ...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-005-9005-0
更新日期：2006-01-01 00:00:00
abstract：:Prior to the 1960s, the model for the molecular structure of cell membranes consisted of a lipid bilayer held in place by a thin film of electrostatically-associated protein stretched over the bilayer surface: (the Danielli-Davson-Robertson "unit membrane" model). Andrew Benson, an expert in the lipids of chloroplast ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-007-9159-z
更新日期：2007-05-01 00:00:00
abstract：:In this brief report, we provide a pictorial essay on an international conference "Photosynthesis Research for Sustainability-2013 in honor of Jalal A. Aliyev" that was held in Baku, Azerbaijan, during June 5-9, 2013 ( http://photosynthesis2013.cellreg.org/ ). We begin this report with a brief note on Jalal Aliyev, th...
journal_title：Photosynthesis research
pub_type：
doi：10.1007/s11120-013-9901-7
更新日期：2013-12-01 00:00:00
abstract：:The chlorophyll-protein and polypeptide composition of manganese deficient and control sugar beet thylakoids was examined using three different detergent-electrophoresis systems. On a per chlorophyll basis, manganese deficiency reduced the amounts of CPa complex (separated by sodium dodecylsulfate (SDS)-polyacrylamide...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00014677
更新日期：1986-01-01 00:00:00
abstract：:In this Minireview, we discuss a number of issues on the primary photosynthetic reactions of the green plant Photosystem II. We discuss the origin of the 683 and 679 nm absorption bands of the PS II RC complex and suggest that these forms may reflect the single-site spectrum with dominant contributions from the zero-p...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1006468024245
更新日期：2000-01-01 00:00:00
abstract：:The dependence of chlorosome development on bacteriochlorophyll (BChl)c synthesis was studied with the phototrophic green bacteriumChloroflexus aurantiacus. By selecting defined culture conditions, three possibilities could be identified. Upon addition of 5-aminolevulinic acid, cells of resting cultures increased thei...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF02184154
更新日期：1994-07-01 00:00:00
abstract：:We have previously investigated the response mechanisms of photosystem II complexes from spinach to strong UV and visible irradiations (Wei et al J Photochem Photobiol B 104:118-125, 2011). In this work, we extend our study to the effects of strong light on the unusual cyanobacterium Acaryochloris marina, which is abl...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-013-9946-7
更新日期：2013-11-01 00:00:00
abstract：:Macroalgae occur in the marine benthos from the upper intertidal to depths of more than 200 m, contributing up to 1 Pg C per year to global primary productivity. Freshwater macroalgae are mainly green (Chlorophyta) with some red (Rhodophyta) and a small contribution of brown (Phaeophyceae) algae, while in the ocean al...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-012-9768-z
更新日期：2012-09-01 00:00:00
abstract：:Oxygenic photosynthesis requires chlorophyll (Chl) for the absorption of light energy, and charge separation in the reaction center of photosystem I and II, to feed electrons into the photosynthetic electron transfer chain. Chl is bound to different Chl-binding proteins assembled in the core complexes of the two photo...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-015-0154-5
更新日期：2015-12-01 00:00:00
abstract：:The PAM-2000 portable chlorophyll fluorometer represents one of the first commercially available instruments utilizing the Pulse Amplitude Modulation (PAM) measurement principle, and has become a widely used platform for measuring chlorophyll fluorescence in a wide range of study systems. In this paper, we describe a ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-005-9010-3
更新日期：2006-03-01 00:00:00
abstract：:The photosystem II (PSII) manganese-stabilizing protein (PsbO) is known to be the essential PSII extrinsic subunit for stabilization and retention of the Mn and Cl(-) cofactors in the oxygen evolving complex (OEC) of PSII, but its function relative to Ca(2+) is less clear. To obtain a better insight into the relations...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-011-9703-8
更新日期：2011-12-01 00:00:00
