Abstract:
:The crystallization of a given protein is a hard task being even more complicated when the protein shows a hydrophobic behavior. In the case of photosynthetic proteins, the difficulty of the experiments increased due to the high light sensitivity. Aqueous solutions of photosystem II core complex (OEC PSII) of Pisum sativum were screened for crystallization conditions using standard crystallization methods. Crystal improvement was achieved by counter-diffusion technique in single capillaries of 0.2 mm inner diameter with a three-layer configuration. The use of this advanced crystallization technique-for the first time applied to the crystallization of membrane proteins-improves the reproducibility of the experiments allowing the initial crystal characterization, and facilitates the manipulation under light protection.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Kutá Smatanová I,Gavira JA,Rezácová P,Vácha F,García-Ruiz JMdoi
10.1007/s11120-007-9131-ysubject
Has Abstractpub_date
2006-12-01 00:00:00pages
255-9issue
3eissn
0166-8595issn
1573-5079journal_volume
90pub_type
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