Abstract：
:Recently, microalgae have attracted attention as sources of biomass energy. However, fatty acids from the microalgae are mainly unsaturated and show low stability in oxygenated environments, due to oxidation of the double bonds. The branched-chain fatty acid, 10-methyl stearic acid, is synthesized from oleic acid in certain bacteria; the fatty acid is saturated, but melting point is low. Thus, it is stable in the presence of oxygen and is highly fluid. We previously demonstrated that BfaA and BfaB in Mycobacterium chlorophenolicum are involved in the synthesis of 10-methyl stearic acid from oleic acid. In this study, as a consequence of the introduction of bfaA and bfaB into the cyanobacterium, Synechocystis sp. PCC 6803, we succeeded in producing 10-methyl stearic acid, with yields up to 4.1% of the total fatty acid content. The synthesis of 10-methyl stearic acid in Synechocystis cells did not show a significant effect on photosynthetic activity, but the growth of the cells was retarded at 34 °C. We observed that the synthesis of 10-methylene stearic acid, a precursor of 10-methyl stearic acid, had an inhibitory effect on the growth of the transformants, which was mitigated under microoxic conditions. Eventually, the amount of 10-methyl stearic acid present in the sulfoquinovosyl diacylglycerol and phosphatidylglycerol of the transformants was remarkably higher than that in the monogalactosyldiacylglycerol and digalactosyldiacylglycerol. Overall, we successfully synthesized 10-methyl stearic acid in the phototroph, Synechocystis, demonstrating that it is possible to synthesize unique modified fatty acids via photosynthesis that are not naturally produced in photosynthetic organisms.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Machida S,Suzuki Idoi
10.1007/s11120-018-0537-5subject
Has Abstractpub_date
2019-03-01 00:00:00pages
173-183issue
1-3eissn
0166-8595issn
1573-5079pii
10.1007/s11120-018-0537-5journal_volume
139pub_type
杂志文章abstract：:The oxidation kinetics under far-red light (FRL) of photosystem I (PSI) high potential donors P700, plastocyanin (PC), and cytochrome f (Cyt f) were investigated in sunflower leaves with the help of a new high-sensitivity photometer at 810 nm. The slopes of the 810 nm signal were measured immediately before and after ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-007-9224-7
更新日期：2007-10-01 00:00:00
abstract：:Significance of molecular crowding in grana thylakoids of higher plants on photosystem II function was studied by 'titrating' the naturally high protein density by fusing unilamellar liposomes of the native lipid mixture with isolated grana membranes (BBY). The incorporation of lipids was monitored by equilibrium dens...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-007-9253-2
更新日期：2008-02-01 00:00:00
abstract：:A three-dimensional model of the core proteins D1 and D2, including the cofactors, that form the Photosystem II reaction centre of pea (Pisum sativum), has been generated. This model was built with a rule-based computer modelling system using the information from the crystal structures of the photosynthetic reaction c...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00033446
更新日期：1992-11-01 00:00:00
abstract：:We investigated several photosynthetic parameters of a virescent mutant of durum wheat and of its wild-type. Electron transport rate to ferricyanide was the same in the two genotypes when expressed on leaf area basis while O2 evolution of the leaf tissue in saturating light and CO2 was slightly higher in the yellow ge...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00033365
更新日期：1989-08-01 00:00:00
abstract：:A combination of inhibitor and (15)N studies were used to investigate the photorespiratory nitrogen cycle in maize, a C4 plant. Inhibitors used included isonicotinyl hydrazide which blocks the conversion of glycine to serine, methionine sulfoximine an inhibitor of GS and azaserine an inhibitor of GOGAT. Results from l...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00041796
更新日期：1983-01-01 00:00:00
abstract：:The PAM-2000 portable chlorophyll fluorometer represents one of the first commercially available instruments utilizing the Pulse Amplitude Modulation (PAM) measurement principle, and has become a widely used platform for measuring chlorophyll fluorescence in a wide range of study systems. In this paper, we describe a ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-005-9010-3
更新日期：2006-03-01 00:00:00
abstract：:Six chlorophyll-protein complexes are isolated from thylakoid membranes of Bryopsis corticulans by dodecyl-beta-D: -maltoside polyacrylamide gel electrophoresis. Unlike that of higher plants, the 77 K fluorescence emission spectrum of the CP1 band, the PSI core complexes of B. corticulans, presents two peaks, one at 6...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-007-9286-6
更新日期：2008-04-01 00:00:00
abstract：:Cells of the green alga Dunaliella tertiolecta grown in a light/dark cycle were exposed to high light for about 15 min. In light, energy-dependent quenching reduced fluorescence emission and decreased PS II efficiency. Within 3 minutes after darkening fluorescence quenching largely relaxed. However, PS II fluorescence...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00033120
更新日期：1996-12-01 00:00:00
abstract：:Ongoing work has led to the identification of most of the biochemical steps in carotenoid biosynthesis in chlorophototrophic bacteria. In carotenogenesis, a relatively small number of modifications leads to a great diversity of carotenoid structures. This review examines the individual steps in the pathway, discusses ...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-008-9312-3
更新日期：2008-08-01 00:00:00
abstract：:This contribution gives a brief survey of the short history since the discovery of the aerobic phototrophic bacteria to focus on a general evolutionary scenario. Most of the citations are of reviews that have covered the earlier literature and to which the reader is directed at appropriate places in the following text...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1020493518379
更新日期：2002-01-01 00:00:00
abstract：:This article gives an historical overview of our group's research on various metabolite translocators of chloroplasts, such as the translocators for phosphorylated intermediates of the Calvin-Benson cycle and of glycolysis, of ADP and ATP, of dicarboxylates, of pyruvate and of hexoses; how it began and where it led to...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1020475803784
更新日期：2002-01-01 00:00:00
abstract：:The major light-harvesting system in cyanobacteria, the phycobilisome, is an essential component of the photosynthetic apparatus that regulates the utilization of the natural light source-the Sun. Earlier works revealed that the thylakoid membrane composition and its physical properties might have an important role in...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-020-00776-1
更新日期：2020-08-01 00:00:00
abstract：:Chloroplast NADP-dependent malate dehydrogenase (NADP-MDH, EC 1.1.1.82) is inactive in the dark and activated in the light via a reduction of specific disulfides by thiol-disulfide interchange with thioredoxin, reduced by the photosynthetic electron transfer. Compared to the constitutively active NAD-dependent forms, ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1016099228450
更新日期：2002-01-01 00:00:00
abstract：:Exposure of the red alga Porphyra perforata or leaves of Phytolacca americana and Echinodorus sp. to white light equivalent to full sunlight for short periods induced large decreases of variable fluorescence measured at 695 nm at 77K. This change was not produced by photoinhibition but rather appeared to result from a...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00118298
更新日期：1986-01-01 00:00:00
abstract：:Mike Wasielewski's pioneering work on Photosystem II photochemistry has an important place in the history of photosynthesis; we are proud to have been associated with him in making those first measurements. Here, we present our association and publications with him, and provide some of the history behind this research...
journal_title：Photosynthesis research
pub_type： 传,历史文章,杂志文章
doi：10.1007/s11120-009-9505-4
更新日期：2010-01-01 00:00:00
abstract：:Roderick K. Clayton passed away on October 23, 2011, at the age of 89, shortly after the plan for this dedicatory issue of Photosynthesis Research had been hatched. I had just written a lengthy letter to him to re-establish contact after a hiatus of 2 or 3 years, and to suggest that I visit him to talk about his life....
journal_title：Photosynthesis research
pub_type： 传,历史文章,杂志文章
doi：10.1007/s11120-013-9948-5
更新日期：2014-05-01 00:00:00
abstract：:In vitro studies of the carotenoid peridinin, which is the primary pigment from the peridinin chlorophyll-a protein (PCP) light harvesting complex, showed a strong dependence on the lifetime of the peridinin lowest singlet excited state on solvent polarity. This dependence was attributed to the presence of an intramol...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-005-1447-x
更新日期：2005-11-01 00:00:00
abstract：:The linear dichroism of Photosystem I particles containing 10 chlorophylls per P700 has been investigated at 10 K. The particles were oriented by uniaxial squeezing of polyacrylamide gels. The oxidation state of P700 was altered either by incubation of the gels with redox mediators or by low temperature illumination. ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00047172
更新日期：1989-07-01 00:00:00
abstract：:Ferredoxin and the flavoprotein, ferredoxin: NADP reductase, have been covalently linked by incubation in the presence of a water soluble carbodiimide. The cross-linking reaction yields an adduct having a 1:1 stoichiometry. The adduct has depressed levels of diaphorase and NADPH oxidase activity and is inactive in red...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00035450
更新日期：1988-09-01 00:00:00
abstract：:The chloroplast photo-oxidation and the expression of the Cab gene Lhcb1, encoding the Lhcb1 light-harvesting chlorophyll a/b-protein of PS II, have been studied in leaf cells of maize treated with the two bleaching herbicides norflurazon and amitrole and of the two carotenoid-free mutants vp9 and vp2 grown under high...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1006477215572
更新日期：2000-01-01 00:00:00
abstract：:We have compared the properties of a mutant of barley lacking Photosystem I (viridis-zb (63) ) with the corresponding wild type using modulated fluorescence measurements. The mutant showed two unexpected characteristics. Firstly, there was a slow decline in the fluorescence signal in the light which was dependent on t...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00016561
更新日期：1993-03-01 00:00:00
abstract：:Cyanobacteria exhibit a novel form of non-photochemical quenching (NPQ) at the level of the phycobilisome. NPQ is a process that protects photosystem II (PSII) from possible highlight-induced photo-damage. Although significant advancement has been made in understanding the NPQ, there are still some missing details. Th...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-018-0498-8
更新日期：2018-08-01 00:00:00
abstract：:A proton electrochemical potential across the membranes of photosynthetic purple bacteria is established by a light-driven proton pump mechanism: the absorbed light in the reaction center initiates electron transfer which is coupled to the vectorial displacement of protons from the cytoplasm to the periplasm. The stoi...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF02185435
更新日期：1993-07-01 00:00:00
abstract：:In thylakoid membranes, several peptides of high MW† are present which may interfere with the study of CP1's components. Modifying Cleveland's technique [7] for limited proteolysis, we have characterized the polypeptides found in the 60 kD region. Some may result from incomplete washing of the CF1 while others come fr...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00020071
更新日期：1980-03-01 00:00:00
abstract：:Recent magnetic-resonance work on YŻ suggests that this species exhibits considerable motional flexibility in its functional site and that its phenol oxygen is not involved in a well-ordered hydrogen-bond interaction (Tang et al., submitted; Tommos et al., in press). Both of these observations are inconsistent with a ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00020428
更新日期：1995-11-01 00:00:00
abstract：:The picture presently emerging from studies on the mechanism of photosystem II electron transport is discussed. The reactions involved in excitation trapping, charge separation and stabilization of the charge pair in the reaction center, followed by the reactions with the substrates, plastoquinone reduction and water ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00032785
更新日期：1985-01-01 00:00:00
abstract：:Bill Arnold's concept of photosynthesis as a solid state, as opposed to solution, phenomenon led him to an amazing series of fundamental discoveries. ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00040990
更新日期：1996-05-01 00:00:00
abstract：:Oxygen evolving photosystem II particles were exposed to 100 and 250 W m(-2) white light at 20°C under aerobic, anaerobic and strongly reducing (presence of dithionite) conditions. Three types of photoinactivation processes with different kinetics could be distinguished: (1) The fast process which occurs under strongl...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00030061
更新日期：1990-01-01 00:00:00
abstract：:Historically, two main concepts guided research into possible mechanisms of light-induced atomic rearrangements in oxygenic photosynthesis: Photodecomposition of CO2 and photodecomposition of water. Both concepts envisioned photoinduced transfers of cumbersome whole atoms and not, as is currently held, photoinduced el...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00036216
更新日期：1991-09-01 00:00:00
abstract：:A chlorosome is an antenna complex located on the cytoplasmic side of the inner membrane in green photosynthetic bacteria that contains tens of thousands of self-assembled bacteriochlorophylls (BChls). Green bacteria are known to incorporate various esterifying alcohols at the C-17 propionate position of BChls in the ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-014-0017-5
更新日期：2014-10-01 00:00:00
