Abstract:
:Cells of the green alga Dunaliella tertiolecta grown in a light/dark cycle were exposed to high light for about 15 min. In light, energy-dependent quenching reduced fluorescence emission and decreased PS II efficiency. Within 3 minutes after darkening fluorescence quenching largely relaxed. However, PS II fluorescence emission decreased again after further darkening. Fo and Fm decreased to the same relative extent and the PS II efficiency was not reduced. This Reduction in Fluorescence yield in Darkness, termed RFD for the purpose of this paper, lasted about 20 min. The deepoxidation state of xanthophylls remained unchanged during and after the 15-min exposure to high light. We show that RFD is insensitive to the uncoupler nigericin and thus unrelated to energy-dependent quenching. RFD correlated with a reduction of the PQ pool after darkening and low levels of far red or blue light (430 nm more than 460 nm) prevented RFD. This is in contrast to observations in higher plants, where a post-illumination reduction of the PQ pool causes and increase in Fo (Groom et al. (1993) Photosynth Res 36: 205-215). Changes in the adenylate energy charge were not correlated with RFD. Antimycin A and cyanide, both inhibitors of the PQ-oxidase, caused an increase in RFD whereas SHAM, an inhibitor of the chloroplastic glycolate-quinone oxidoreductase, caused a decrease. Low CO2 concentrations, known to increase the oxygenase activity of Rubisco and to generate glycolate and P-glycolate in light, caused an increase in RFD. We propose that accumulated glycolate and P-glycolate reduce the PQ pool in darkness, leading to the formation of RFD. During RFD, 77 K fluorescence emission from PS II was more reduced than that from PS I, thus resembling a state I, state II transition. However, the reduction in fluorescence yield during RFD is much larger than the reduction previously attributed to state transitions and it is unclear whether RFD and state transitions are identical. The formation and relaxation of RFD increased with higher temperatures and the extent of RFD was largest at the growth temperature (25°C). RFD has to be taken into account when fluorescence is measured after darkening as it may be mistaken for energy-dependent quenching.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Casper-Lindley C,Björkman Odoi
10.1007/BF00033120subject
Has Abstractpub_date
1996-12-01 00:00:00pages
209-22issue
3eissn
0166-8595issn
1573-5079journal_volume
50pub_type
杂志文章abstract::A spontaneous mutant (R/89) of photosynthetic purple bacterium Rhodobacter sphaeroides R-26 was selected for resistance to 200 μM atrazin. It showed increased resistance to interquinone electron transfer inhibitors of o-phenanthroline (resistance factor, RF=20) in UQo reconstituted isolated reaction centers and terbut...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00032585
更新日期:1995-08-01 00:00:00
abstract::The rate and extent of light activation of PEPC may be used as another criterion to distinguish C3 and C4 plants. Light stimulated phosphoenolypyruvate carboxylase (PEPC) in leaf discs of C4 plants, the activity being three times greater than that in the dark but stimulation of PEPC was limited about 30% over the dark...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00015061
更新日期:1993-10-01 00:00:00
abstract::Flash-induced kinetics of the membrane potential increase related to electron transfer within the cytochrome (cyt) b/c1 complex (Phase III) and that of cyt c1+c2 reduction have been measured as a function of myxothiazol concentration in isolated chromatophores and whole cells of Rhodobacter sphaeroides. Upon addition ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00041020
更新日期:1996-05-01 00:00:00
abstract::Ongoing work has led to the identification of most of the biochemical steps in carotenoid biosynthesis in chlorophototrophic bacteria. In carotenogenesis, a relatively small number of modifications leads to a great diversity of carotenoid structures. This review examines the individual steps in the pathway, discusses ...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-008-9312-3
更新日期:2008-08-01 00:00:00
abstract::The interaction of PsaC with the core heterodimer of Photosystem 1 was studied in wild type Synechocystis sp. PCC 6803 and the site-directed mutant R561E of PsaB. The mutant reaction center was much less stable in urea and the functional reconstitution of the mutant core using PsaC was impaired. However, the extent of...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00018261
更新日期:1994-12-01 00:00:00
abstract::Photosynthetic electron transfer has been examined in whole cells, isolated membranes and in partially purified reaction centers (RCs) of Roseicyclus mahoneyensis, strain ML6 and Porphyrobacter meromictius, strain ML31, two species of obligate aerobic anoxygenic phototrophic bacteria. Photochemical activity in strain ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-011-9718-1
更新日期:2012-02-01 00:00:00
abstract::The authors present a study of the fluorescence and absorbance transients occurring in whole cells of purple nonsulfur bacterium Rhodobacter sphaeroides on the millisecond timescale under pulsed actinic illumination. The fluorescence induction curve is interpreted in terms of combination of effects of redox changes in...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-010-9569-1
更新日期:2010-08-01 00:00:00
abstract::Cyanobacteria, algae, and plants produce dioxygen from water. Driven and clocked by light quanta, the catalytic Mn(4)Ca Tyrosine centre accumulates four oxidizing equivalents before it abstracts four electrons from water and liberates dioxygen and protons. Intermediates of this reaction cascade are short-lived (<100 m...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-008-9321-2
更新日期:2008-10-01 00:00:00
abstract::Four Indica and five Japonica varieties of rice (Oryza sativa L.) were examined to elucidate their differences in photosynthetic activity and dark respiratory rate as influenced by leaf nitrogen levels and temperatures. The photosynthetic rates of single leaf showed correlations with total nitrogen and soluble protein...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00032321
更新日期:1987-01-01 00:00:00
abstract::A computer model comprising light reactions in PS II and PS I, electron-proton transport reactions in mesophyll and bundle sheath chloroplasts, all enzymatic reactions and most of the known regulatory functions of NADP-ME type C(4) photosynthesis has been developed as a system of differential budget equations for inte...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1010695402963
更新日期:2000-01-01 00:00:00
abstract::The xanthophyll cycle represents one of the important photoprotection mechanisms in plant cells. In the present review, we summarize current knowledge about the violaxanthin cycle of vascular plants, green and brown algae, and the diadinoxanthin cycle of the algal classes Bacillariophyceae, Xanthophyceae, Haptophyceae...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-010-9536-x
更新日期:2010-11-01 00:00:00
abstract::The 3- and 7-formyl groups of chlorophyll-d (Chl-d) and bacteriochlorophyll-e (BChl-e), respectively, were regioselectively labeled with an isotopically stable oxygen-18 (¹⁸O) atom to give 3¹-¹⁸O-labeled Chl-d and 7¹-¹⁸O-labeled BChl-e (ca. 90% ¹⁸O) by exchanging the carbonyl oxygen atoms in the presence of acidic H₂ ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-010-9590-4
更新日期:2010-09-01 00:00:00
abstract::The role of the ferredoxin:thioredoxin system in the reversible light activation of chloroplast enzymes by thiol-disulfide interchange with thioredoxins is now well established. Recent fruitful collaboration between biochemists and structural biologists, reflected by the shared authorship of the paper, allowed to solv...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/B:PRES.0000017194.34167.6d
更新日期:2004-01-01 00:00:00
abstract::Photosynthesis and carbohydrate metabolism of higher plants need to be tightly regulated to prevent tissue damage during environmental changes. The intracellular position of chloroplasts changes due to a changing light regime. Chloroplast avoidance and accumulation response under high and low light, respectively, are ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-020-00795-y
更新日期:2021-01-01 00:00:00
abstract::In this paper we compared the pigment composition, photochemical activity, chloroplast ultrastructure, thylakoid membrane polypeptide composition and ribosomal content of wild-type and seven light-sensitive mutants of Chlamydomonas reinhardii.All the mutants had low chlorophyll and carotenoid content compared to wild-...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00032261
更新日期:1982-01-01 00:00:00
abstract::Soybean [Glycine max (L.) Merr. cv. Williams 82 and A3127] plants were grown in the field under long-term soil moisture deficit and irrigation to determine the effects of severe drought stress on the photosynthetic capacity of soybean leaves. Afternoon leaf water potentials, stomatal conductances, intercellular CO2 co...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00051735
更新日期:1990-07-01 00:00:00
abstract::Heliobacteria are a recently discovered group of anoxygenic phototrophic bacteria, first described in 1983. Heliobacteria contain bacteriochlorophyll g, a pigment unique to species of this group, and synthesize the simplest photosynthetic complexes of all known phototrophs. Also, unlike all other phototrophs, heliobac...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-009-9516-1
更新日期:2010-06-01 00:00:00
abstract::Carbohydrate feedstocks are at the root of bioindustrial production and are needed in greater quantities than ever due to increased prioritization of renewable fuels with reduced carbon footprints. Cyanobacteria possess a number of features that make them well suited as an alternative feedstock crop in comparison to t...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-014-9980-0
更新日期:2015-03-01 00:00:00
abstract::This study was conducted to more clearly define the physiological effects of PS II herbicides on chloroplast thylakoid membrane activity and composition. Barley (Hordeum vulgare L. cv Boone) was grown in hydroponic culture at 20°C in a growth chamber with a light intensity of 500 μmole photons m(-2) s(-1). Atrazine (2...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00028793
更新日期:1992-04-01 00:00:00
abstract::Acaryochloris marina is a unique cyanobacterium that contains chlorophyll (Chl) d as a major pigment. Because Chl d has smaller excitation energy than Chl a used in ordinary photosynthetic organisms, the energetics of the photosystems of A. marina have been the subject of interest. It was previously shown that the red...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-014-0073-x
更新日期:2015-08-01 00:00:00
abstract::Alternative electron sinks are an important regulatory mechanism to dissipate excessively absorbed light energy particularly under fast changing dynamic light conditions. In diatoms, the cyclic electron transport (CET) around Photosystem II (PS II) is an alternative electron transport pathway (AET) that contributes to...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-015-0209-7
更新日期:2016-05-01 00:00:00
abstract::Unique light-harvesting antennas in the green sulfur bacterium Chlorobaculum tepidum, called chlorosomes, consist of self-aggregates of bacteriochlorophyll (BChl) c. In the biosynthesis of BChl c, BciC demethoxycarbonylase removes the C132-methoxycarbonyl group to facilitate the self-aggregation of BChl c. We previous...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-018-0573-1
更新日期:2019-03-01 00:00:00
abstract::We evaluate low-temperature (low-T) emission spectra of photosystem II core complexes (PSII-cc) previously reported in the literature, which are compared with emission spectra of PSII-cc obtained in this work from spinach and for dissolved PSII crystals from Thermosynechococcus (T.) elongatus. This new spectral datase...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-015-0128-7
更新日期:2015-06-01 00:00:00
abstract::Oxygen evolving photosystem II particles were exposed to 100 and 250 W m(-2) white light at 20°C under aerobic, anaerobic and strongly reducing (presence of dithionite) conditions. Three types of photoinactivation processes with different kinetics could be distinguished: (1) The fast process which occurs under strongl...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00030061
更新日期:1990-01-01 00:00:00
abstract::In studies on photosynthetic systems it is common practice to interpret the results of time-resolved fluorescence experiments on the basis of compartmental, or target, models. Each compartment represents a group of molecules with similar fluorescence characteristics. In cases of practical interest, the members of each...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00014883
更新日期:1996-11-01 00:00:00
abstract::Two species-invariant tryptophan residues at positions 109 and 250 of tobacco Rubisco activase were identified by site-directed mutagenesis as being responsible for the increase in intrinsic fluorescence upon addition of ATP, which has been previously attributed to increased self-association. Substitution of W109, whi...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-006-9051-2
更新日期:2006-05-01 00:00:00
abstract::In recent years the enzymes of the C3 photosynthetic carbon reduction (PCR) cycle have been studied using the techniques of molecular biology. In this review we discuss the primary protein sequences and structural predictions that have been made for a number of these enzymes, which, with the input of crystallographic ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00029971
更新日期:1991-01-01 00:00:00
abstract::Since 1986, non-uniform photosynthesis over the leaf area that may be attributed to patchy stomatal closure, has been an important issue in stress physiology of photosynthesis. In this review, I first outline the gaseous environment within the intercellular spaces, because this is the most fundamental background of th...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00035537
更新日期:1992-03-01 00:00:00
abstract::Using 77 K chlorophyll a (Chl a) fluorescence spectra in vivo, the development was studied of Photosystems II (PS II) and I (PS I) during greening of barley under intermittent light followed by continuous light at low (LI, 50 mumol m(-2) s(-1)) and high (HI, 1000 mumol m(-2) s(-1)) irradiances. The greening at HI inte...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1020943107318
更新日期:2002-01-01 00:00:00
abstract::Biological water oxidation, performed by a single enzyme, photosystem II, is a central research topic not only in understanding the photosynthetic apparatus but also for the development of water splitting catalysts for technological applications. Great progress has been made in this endeavor following the report of a ...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-019-00648-3
更新日期:2019-10-01 00:00:00