Abstract:
:A spontaneous mutant (R/89) of photosynthetic purple bacterium Rhodobacter sphaeroides R-26 was selected for resistance to 200 μM atrazin. It showed increased resistance to interquinone electron transfer inhibitors of o-phenanthroline (resistance factor, RF=20) in UQo reconstituted isolated reaction centers and terbutryne in reaction centers (RF=55) and in chromatophores (RF=85). The amino acid sequence of the QB binding protein of the photosynthetic reaction center (the L subunit) was determined by sequencing the corresponding pufL gene and a single mutation was found (Ile(L229) → Met). The changed amino acid of the mutant strain is in van der Waals contact with the secondary quinone QB. The binding and redox properties of QB in the mutant were characterized by kinetic (charge recombination) and multiple turnover (cytochrome oxidation and semiquinone oscillation) assays of the reaction center. The free energy for stabilization of QAQB (-) with respect to QA (-)QB was ΔGAB=-60 meV and 0 meV in reaction centers and ΔGAB=-85 meV and -46 meV in chromatophores of R-26 and R/89 strains at pH 8, respectively. The dissociation constants of the quinone UQo and semiquinone UQo (-) in reaction centers from R-26 and R/89 showed significant and different pH dependence. The observed changes in binding and redox properties of quinones are interpreted in terms of differential effects (electrostatics and mesomerism) of mutation on the oxidized and reduced states of QB.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Tandori J,Nagy L,Puskás A,Droppa M,Horváth G,Maróti Pdoi
10.1007/BF00032585subject
Has Abstractpub_date
1995-08-01 00:00:00pages
135-46issue
2eissn
0166-8595issn
1573-5079journal_volume
45pub_type
杂志文章abstract::Moderately high temperature reduces photosynthetic capacities of leaves with large effects on thylakoid reactions of photosynthesis, including xanthophyll conversion in the lipid phase of the thylakoid membrane. In previous studies, we have found that leaf temperature of 40°C increased zeaxanthin accumulation in dark-...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-011-9672-y
更新日期:2011-09-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00029737
更新日期:1986-01-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-010-9562-8
更新日期:2010-07-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00042974
更新日期:1995-02-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00027142
更新日期:1994-01-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-012-9770-5
更新日期:2012-09-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00049278
更新日期:1985-09-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1006455230379
更新日期:2000-01-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-004-7311-6
更新日期:2005-06-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-018-0573-1
更新日期:2019-03-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-011-9703-8
更新日期:2011-12-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-005-9004-1
更新日期:2006-03-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-015-0128-7
更新日期:2015-06-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00047079
更新日期:1990-11-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1020447030191
更新日期:2002-01-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00018272
更新日期:1987-01-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-015-0094-0
更新日期:2015-08-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-006-9064-x
更新日期:2006-07-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00032321
更新日期:1987-01-01 00:00:00
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pub_type: 传,历史文章,杂志文章
doi:10.1007/s11120-017-0380-0
更新日期:2017-06-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-014-0047-z
更新日期:2015-08-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00035234
更新日期:1987-01-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1010681721929
更新日期:2001-01-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-017-0417-4
更新日期:2018-03-01 00:00:00
abstract::Roderick K. Clayton passed away on October 23, 2011, at the age of 89, shortly after the plan for this dedicatory issue of Photosynthesis Research had been hatched. I had just written a lengthy letter to him to re-establish contact after a hiatus of 2 or 3 years, and to suggest that I visit him to talk about his life....
journal_title:Photosynthesis research
pub_type: 传,历史文章,杂志文章
doi:10.1007/s11120-013-9948-5
更新日期:2014-05-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF02183048
更新日期:1994-09-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-012-9761-6
更新日期:2012-09-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-013-9964-5
更新日期:2014-03-01 00:00:00
abstract::This review focuses on our recent efforts in synthetic ruthenium-tyrosine-manganese chemistry mimicking the donor side reactions of Photosystem II. Tyrosine and tryptophan residues were linked to ruthenium photosensitizers, which resulted in model complexes for proton-coupled electron transfer from amino acids. A new ...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-005-9005-0
更新日期:2006-01-01 00:00:00
abstract::Cross-reconstitution of the extrinsic proteins and Photosystem II (PS II) from a green alga, Chlamydomonas reinhardtii, and a higher plant,Spinacia oleracea, was performed to clarify the differences of binding properties of the extrinsic proteins between these two species of organisms. (1) Chlamydomonas PsbP and PsbQ ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-004-7760-y
更新日期:2005-06-01 00:00:00