Abstract:
:Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes carboxylation of ribulose-1,5-bisphosphate, the first in a series of reactions leading to the incorporation of atmospheric CO₂ into biomass. Rubisco requires Rubisco activase (RCA), an AAA+ ATPase that reactivates Rubisco by remodelling the conformation of inhibitor-bound sites. RCA is regulated by the ratio of ADP:ATP, with the precise response potentiated by redox regulation of the alpha-isoform. Measuring the effects of ADP on the activation of Rubisco by RCA using the well-established photometric assay is problematic because of the adenine nucleotide requirement of 3-phosphoglycerate (3-PGA) kinase. Described here is a novel assay for measuring RCA activity in the presence of variable ratios of ADP:ATP. The assay couples the formation of 3-PGA from ribulose 1,5-bisphosphate and CO₂ to NADH oxidation through cofactor-dependent phosphoglycerate mutase, enolase, PEP carboxylase and malate dehydrogenase. The assay was used to determine the effects of Rubisco and RCA concentration and ADP:ATP ratio on RCA activity, and to measure the activation of a modified Rubisco by RCA. Variations of the basic assay were used to measure the activation state of Rubisco in leaf extracts and the activity of purified Rubisco. The assay can be automated for high-throughput processing by conducting the reactions in two stages.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Scales JC,Parry MA,Salvucci MEdoi
10.1007/s11120-013-9964-5subject
Has Abstractpub_date
2014-03-01 00:00:00pages
355-65issue
3eissn
0166-8595issn
1573-5079journal_volume
119pub_type
杂志文章abstract::Effect of chemical oxidation by ferricyanide on bacteriochlorophyll a (BChl a) in the Fenna-Matthews-Olson protein (FMO) was studied using absorbance and fluorescence spectroscopy at ambient and cryogenic temperatures. Partially selective oxidation of pigments bound to the antenna complex was achieved and the probable...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-013-9878-2
更新日期:2013-09-01 00:00:00
abstract::The Photosystem I reaction centre protein CP1, isolated from barley using polyacrylamide gel electrophoresis showed an EPR (Electron Paramgnetic Resonance) spectrum with the polarisation pattern AEEAAE, typical of the primary donor triplet state (3)P700, created via radical pair formation and recombination. (3)P700 co...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00034795
更新日期:1992-06-01 00:00:00
abstract::Previous investigations have shown that increased source strength as a result of elevated CO(2) can alter the timing of the phases of change that occur in photosynthetic rates during dicot leaf ontogeny [Miller et al. (1997) Plant Physiol 115: 1195-1200]. To evaluate the converse situation of decreased source strength...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1006367719639
更新日期:2000-01-01 00:00:00
abstract::Photoinhibition of the light-induced Photosystem I (PS I) electron transfer activity from the reduced dichlorophenol indophenol to methyl viologen was studied. PS I preparations with Chl/P700 ratios of about 180 (PS I-180), 100 (PS I-100) and 40 (PS I(HA)-40) were isolated from spinach thylakoid membranes by the treat...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00016175
更新日期:1996-02-01 00:00:00
abstract::NADP:ferredoxin oxidoreductase (EC. 1.6.7.1.) isolated from a thermophilic blue-green alga, Synechococcus sp., was stable at temperatures up to 65°C. The diaphorase and cytochrome c reductase activities of the enzyme were low at 25°C but increased with elevated temperature to reach a maximum at about 60°C. The pH-prof...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00020595
更新日期:1980-09-01 00:00:00
abstract::Photosynthesis was well-established on the earth at least 3.5 thousand million years ago, and it is widely believed that these ancient organisms had similar metabolic capabilities to modern cyanobacteria. This requires that development of two photosystems and the oxygen evolution capability occurred very early in the...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:
更新日期:1992-01-01 00:00:00
abstract::The thylakoid membrane protein PsbS is critical for quenching excessive excitation energy in mechanisms that involve the light-harvesting complexes of photosystem II. Liposomes of thylakoid lipids have been shown to be a very good platform to study photosynthetic membrane proteins and their interactions. In this study...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-015-0176-z
更新日期:2016-01-01 00:00:00
abstract::A time line of important research relating to anoxygenic photosynthetic organisms is presented. The time line includes discoveries of organisms, metabolic capabilities, molecular complexes and genetic systems. It also pinpoints important milestones in our understanding of the structure, function, organization, assembl...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/B:PRES.0000030448.24695.ec
更新日期:2004-01-01 00:00:00
abstract::The discovery of a new maize (Zea mays L.) transposon system, Mutator, and the cloning of the 1.4 kilobase transposon, Mul, have made feasible the isolation of nuclear photosynthetic genes which are recognized only by their mutant phenotype. Mutant maize plants which express a high chlorophyll fluorescent (hcf) phenot...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00042979
更新日期:1988-10-01 00:00:00
abstract::A hypothesis is proposed to explain the function of Cl(-) in activating the oxygenevolving complex (OEC) of photosystem II (PS II), based on the results of recent (35)Cl-NMR studies. The putative mechanism involves Cl(-) binding to two types of sites. An intrinsic site is suggested to be composed of three histidyl res...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00029400
更新日期:1987-09-01 00:00:00
abstract::Light-induced reaction dynamics of isolated photosynthetic membranes obtained from wild-type (WT) and reaction center (RC)-subunit deletion strains SPUHK1 (an H-subunit deletion mutant) and SK Delta LM (an (L+M) deletion mutant) of the purple non-sulphur bacterium Rhodospirillum rubrum have been investigated by femtos...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-009-9520-5
更新日期:2010-03-01 00:00:00
abstract::After a brief prologue on Otto Kandler's life, we describe briefly his pioneering work on photosynthesis (photophosphorylation and the carbon cycle) and his key participation in the discovery of the concept of three forms of life (Archaea, Prokarya, and Eukarya). With Otto Kandler's passing, both the international pho...
journal_title:Photosynthesis research
pub_type: 传,历史文章,杂志文章
doi:10.1007/s11120-018-0530-z
更新日期:2018-09-01 00:00:00
abstract::In this communication, we report our progress on the development of low-frequency Fourier transform infrared (FTIR) spectroscopic techniques to study metal-substrate and metal-ligand vibrational modes in the Photosystem II/oxygen-evolving complex (PS II/OEC). This information will provide important structural and mech...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1010779327960
更新日期:2000-01-01 00:00:00
abstract::We present the wavelength dependence of homogeneous holewidths of persistent spectral holes burnt in O2-evolving Photosystem II core complexes isolated from spinach, in the temperature range 2.5-8 K. The data supports the assignment that those chlorophylls which undergo persistent spectral hole-burning are specific CP...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-004-7927-6
更新日期:2005-06-01 00:00:00
abstract::Polyamines (PAs) are small metabolites that are produced and oxidized in chloroplasts with an obscure mode of action. Recently, we showed that qE is stimulated by PAs in higher plants (Nicotiana tabacum) and in genetically modified plants with elevated thylakoid-associated PAs (Ioannidis and Kotzabasis Biochim Biophys...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-010-9617-x
更新日期:2011-02-01 00:00:00
abstract::A simple approach to determine CO2/O2 specificity factor (τ) of ribulose 1,5-bisphosphate carboxylase/oxygenase is described. The assay measures the amount of CO2 fixation at varying [CO2]/[O2] ratios after complete consumption of ribulose 1,5-bisphosphate (RuBP). Carbon dioxide fixation catalyzed by the carboxylase w...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00029463
更新日期:1995-01-01 00:00:00
abstract::Taro and cocoyam were grown outdoors in either full sun or under 40% shade. Leaves were tagged as they emerged and the effect of leaf age on net CO2 assimilation rate (A) was determined. The effects of shading on A, transpiration (E), stomatal conductance for CO2 (gc) and H2O (gs), and water use efficiency (WUE) were ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00055064
更新日期:1987-01-01 00:00:00
abstract::Photosynthesis powers our planet and is a source of inspiration for developing artificial constructs mimicking many aspects of the natural energy transducing process. In the complex machinery of photosystem II (PSII), the redox activity of the tyrosine Z (Tyrz) hydrogen-bonded to histidine 190 (His190) is essential fo...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-020-00815-x
更新日期:2021-01-11 00:00:00
abstract::Characteristics of primary phases in chlorophyll a fluorescence transients based on room temperature in vivo measurement with a Plant Productivity Fluorometer (Brancker Model SF-10) can be greatly facilitated by coupling the instrument to a fast data acquisition system. The SF-10 was linked to a Multitech Industrial C...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00041817
更新日期:1983-01-01 00:00:00
abstract::The possible origins of the different fluorescence decay components in green plants are discussed in terms of a random walk and Butler's bipartite model. The interaction of the excitations with the photosystem II reaction centers and, specifically, the regeneration of theses excitations by charge recombination within ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00118288
更新日期:1986-01-01 00:00:00
abstract::We present here our tribute to an outstanding unique scientist, a pioneer of plant biochemistry, best known for his seminal ground-breaking discoveries related to electron and proton transport, and their intimate relation to ATP synthesis in chloroplasts. His dedication and in-depth understanding of science was matche...
journal_title:Photosynthesis research
pub_type: 传,历史文章,杂志文章
doi:10.1007/s11120-017-0380-0
更新日期:2017-06-01 00:00:00
abstract::In this paper, we describe a protocol to obtain a site-directed mutants in thepsbA gene ofChlamydomonas reinhardtii, which overcomes several drawbacks of previous protocols, and makes it possible to generate a mutant within a month. Since the large size of the gene, and the presence of four large introns has made mole...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF02187123
更新日期:1994-11-01 00:00:00
abstract::Illumination of the dark-incubated cells of Synechocystis PCC6803 caused recovery of both respiratory activity of oxygen uptake and PS I-cyclic electron flow, which was monitored by the dark reduction of P700(+) in the presence of DCMU after a 50 ms pulse light (MT) under background far-red light, but the effects were...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1017946524199
更新日期:2001-01-01 00:00:00
abstract::We generated transgenic tobacco plants with high levels of fructose-1,6-bisphosphatase expressing cyanobacterialfructose-1,6-/sedoheptulose-1,7-bisphosphatase in the cytosol. At ambient CO(2) levels (360 ppm), growth, photosynthetic activity, and fresh weight were unchanged but the sucrose/hexose/starch ratio was slig...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-011-9645-1
更新日期:2011-05-01 00:00:00
abstract::Cuttings of grapevine (Vitis vinifera L. cv. Chardonnay) were dark-forced at least three weeks. Pigment contents, 77 K fluorescence emission, excitation spectra of the leaves, petioles, stems, transmission electron micrographs of the etioplasts from leaves, the chlorenchyma tissues of the stems were analysed. The dark...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-004-1061-3
更新日期:2004-01-01 00:00:00
abstract::Relationships between induced high leaf intercellular CO₂ concentrations, leaf K⁺ and NO₃⁻ ion movement and early fruit formation under macronutrient limitation are not well understood. We examined the effects and interactions of reduced K/N input treatments on leaf intercellular CO₂, photosynthesis rate, carboxylatio...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-013-9832-3
更新日期:2013-07-01 00:00:00
abstract::Joan Mary Anderson, known to most people as Jan, was born on May 12, 1932 in Dunedin, New Zealand. She died on August 28, 2015 in Canberra, Australia. To celebrate her life, we present here a brief biography, some comments on her discoveries in photosynthesis during a career spanning more than half a century, and remi...
journal_title:Photosynthesis research
pub_type: 传,历史文章,杂志文章
doi:10.1007/s11120-016-0287-1
更新日期:2016-08-01 00:00:00
abstract::Chlorobaculum [Cba.] tepidum is known to grow optimally at 48-52 °C and can also be cultured at ambient temperatures. In this paper, we prepared constant temperature, temperature shift, and temperature shift followed by backshift cultures and investigated the intrinsic properties and spectral features of chlorosomes f...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-013-9800-y
更新日期:2013-05-01 00:00:00
abstract::The main objective of the present review is to provide a compilation of published data of the effects of several climatic conditions on Rubisco, particularly its activity, state of activation, and concentration, and its influence on leaf gas exchange and photosynthesis. The environmental conditions analyzed include dr...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-013-9861-y
更新日期:2013-11-01 00:00:00
abstract::A combination of inhibitor and (15)N studies were used to investigate the photorespiratory nitrogen cycle in maize, a C4 plant. Inhibitors used included isonicotinyl hydrazide which blocks the conversion of glycine to serine, methionine sulfoximine an inhibitor of GS and azaserine an inhibitor of GOGAT. Results from l...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00041796
更新日期:1983-01-01 00:00:00