Abstract：
:Photoinhibition of the light-induced Photosystem I (PS I) electron transfer activity from the reduced dichlorophenol indophenol to methyl viologen was studied. PS I preparations with Chl/P700 ratios of about 180 (PS I-180), 100 (PS I-100) and 40 (PS I(HA)-40) were isolated from spinach thylakoid membranes by the treatments with Triton X-100, followed by sucrose density gradient centrifugation and hydroxylapatite column chromatography. White light irradiation (1.1 × 10(4)μE m(-2) s(-1)) of PS I-180 for 2 hours bleached 50% of the chlorophyll and caused a 58% decrease in the electron transfer activity with virtually no loss of the primary donor, P700. The flash-induced absorbance change showed the decay phase with a half time of about 10 μs that was attributed to the P700 triplet, suggesting that the photoinhibitory light treatment caused the destruction of the PS I acceptor(s), Fx and possibly A1. PS I-100 was similarly photobleached by the irradiation and the electron transfer activity decreased. There was, however, no apparent photoinhibition of the electron transport activity in PS I(HA)-40. Photoinhibition similar to that seen in PS I-180 also occurred in membrane fragments that were isolated without any detergent from a PS II-deficient mutant strain of the cyanobacterium Synechocystis sp. PCC 6803. PS I-180 was not photoinhibited under anaerobic conditions. The production of superoxide and fatty acid hydroperoxide during white light irradiation was significantly greater in PS I-180 than in PS I(HA)-40. The mechanism of photoinhibition in PS I preparations is discussed in relation to the formation of toxic oxygen molecules.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Baba K,Itoh S,Hastings G,Hoshina Sdoi
10.1007/BF00016175subject
Has Abstractpub_date
1996-02-01 00:00:00pages
121-30issue
2eissn
0166-8595issn
1573-5079journal_volume
47pub_type
杂志文章abstract：:The relationship between the redox state of P700, the primary donor of PS I, monitored using absorbance changes at 830 nm and photochemical energy storage in PS I reaction centers assayed with the photoacoustic method (PA) was studied in isolated PS I submembrane particles aspirated onto nitrocellulose filters. Severa...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1021237026500
更新日期：2002-01-01 00:00:00
abstract：:Two-component regulatory systems that respond to changes in redox potential have recently been discovered in bacteria. 'Redox sensors' are defined as electron carriers which initiate control of gene expression upon oxidation or reduction. 'Redox response regulators' are defined as DNA-binding proteins which modify gen...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00016274
更新日期：1993-05-01 00:00:00
abstract：:Flash-induced absorbance measurements at 830 nm on both nanosecond and microsecond timescales have been used to characterise the effect of ultraviolet light on Photosystem II core particles. A combination of UV-A and UV-B, closely simulating the spectrum of sunlight below 350 nm, was found to have a primary effect on ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00029424
更新日期：1996-07-01 00:00:00
abstract：:The polypeptide composition of thylakoid membranes of the red alga Cyanidium caldarium was studied by PAGE in the presence of lithium dodecyl sulfate. The thylakoid membranes were shown to contain 65 polypeptides with mol wt from 110 to 10 kDa. PS I isolated from C. caldarium cells is composed of at least 5 components...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00035678
更新日期：1991-10-01 00:00:00
abstract：:Chlorophyll f is a photosynthetic pigment that was discovered in 2010. In this study, we present investigations on spectral and dynamic characteristics of singlet-excited and triplet states of Chl f with the application of ultrafast time-resolved absorption and fluorescence spectroscopies. The pigment was studied at r...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-014-9981-z
更新日期：2014-07-01 00:00:00
abstract：:When excited by ultraviolet radiation, leaves of a great number of species of higher plants exhibit emission of blue fluorescence, comparable in intensity to the red emission of chlorophyll. The fluorescence decay of the blue emission of spinach leaves recorded by single photon counting techniques is decomposed into e...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00033171
更新日期：1990-09-01 00:00:00
abstract：:Relationships between induced high leaf intercellular CO₂ concentrations, leaf K⁺ and NO₃⁻ ion movement and early fruit formation under macronutrient limitation are not well understood. We examined the effects and interactions of reduced K/N input treatments on leaf intercellular CO₂, photosynthesis rate, carboxylatio...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-013-9832-3
更新日期：2013-07-01 00:00:00
abstract：:Most chloroplasts undergo changes in composition, function and structure in response to growth irradiance. However, Tradescantia albiflora, a facultative shade plant, is unable to modulate its light-harvesting components and has the same Chl a/Chl b ratios and number of functional PS II and PS I reaction centres on a ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1010681721929
更新日期：2001-01-01 00:00:00
abstract：:In many ways, the 1963 conference at Airlie House was an unusual event. The meeting convincingly demonstrated that photosynthetic research, previously reported mostly at conferences of botany and/or plant physiology, had become an independent and important field of biological research. New techniques had been develope...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/B:PRES.0000030427.35645.5c
更新日期：2004-01-01 00:00:00
abstract：:In this paper, we describe a protocol to obtain a site-directed mutants in thepsbA gene ofChlamydomonas reinhardtii, which overcomes several drawbacks of previous protocols, and makes it possible to generate a mutant within a month. Since the large size of the gene, and the presence of four large introns has made mole...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF02187123
更新日期：1994-11-01 00:00:00
abstract：:Earlier mass spectrometric measurements, in which oxygen evolution was measured following short saturating light flashes, indicated that with a time resolution of about 30 s no form of bound water and/or an oxidation product exists up to the redox state S3 of the oxygen evolving center (R. Radmer and O. Ollinger, 1986...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00046761
更新日期：1993-01-01 00:00:00
abstract：:A chlorosome is an antenna complex located on the cytoplasmic side of the inner membrane in green photosynthetic bacteria that contains tens of thousands of self-assembled bacteriochlorophylls (BChls). Green bacteria are known to incorporate various esterifying alcohols at the C-17 propionate position of BChls in the ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-014-0017-5
更新日期：2014-10-01 00:00:00
abstract：:Chlorobium tepidum is a Gram-negative bacterium of the green sulfur phylum (Chlorobia). Chlorobia are obligate anaerobic photolithoautotrophs that are widely distributed in aquatic environments where anoxic layers containing reduced sulfur compounds are exposed to light. The envelope of C. tepidum is a complex organel...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-010-9544-x
更新日期：2010-06-01 00:00:00
abstract：:Triplet state electron paramagnetic resonance (EPR) experiments have been carried out at X-band on Rb. sphaeroides R-26 reaction centers that have been reconstituted with the carotenoid, spheroidene, and exchanged with 13(2)-OH-Zn-bacteriochlorophyll a and [3-vinyl]-13(2)-OH-bacteriochlorophyll a at the monomeric, 'ac...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00032823
更新日期：1993-09-01 00:00:00
abstract：:Nonlinear optical responses of bacteriochlorophyll a (BChl a) were investigated by means of the three-pulse four-wave mixing (FWM) technique under the resonant excitation into the Q ( y ) band. The experimental results are explained by a theoretical model calculation including the Brownian oscillation mode of the solv...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-007-9266-x
更新日期：2008-02-01 00:00:00
abstract：:Various techniques of electron microscopy (EM) such as ultrathin sectioning, freeze-fracturing, freeze-etching, negative staining and (cryo-)electron crystallography of two-dimensional crystals have been employed, since now, to obtain much of the structural information of the Photosystem II (PS II) pigment-protein com...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1024927631196
更新日期：2003-01-01 00:00:00
abstract：:A hypothesis is proposed to explain the function of Cl(-) in activating the oxygenevolving complex (OEC) of photosystem II (PS II), based on the results of recent (35)Cl-NMR studies. The putative mechanism involves Cl(-) binding to two types of sites. An intrinsic site is suggested to be composed of three histidyl res...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00029400
更新日期：1987-09-01 00:00:00
abstract：:Exposure of the red alga Porphyra perforata or leaves of Phytolacca americana and Echinodorus sp. to white light equivalent to full sunlight for short periods induced large decreases of variable fluorescence measured at 695 nm at 77K. This change was not produced by photoinhibition but rather appeared to result from a...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00118298
更新日期：1986-01-01 00:00:00
abstract：:The quantum yield for light-induced H2 generation was measured for a previously optimized bio-hybrid cytochrome c 6-crosslinked PSI(C13G)-1,8-octanedithiol-[FeFe]-H2ase(C97G) (PSI-H2ase) nanoconstruct. The theoretical quantum yield for the PSI-H2ase nanoconstruct is 0.50 molecules of H2 per photon absorbed, which equa...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-014-0064-y
更新日期：2016-01-01 00:00:00
abstract：:Two lessons I have learned during my research career are the importance of following up unexpected observations and realizing that the most obvious interpretation of such observations can be rational but wrong. When you carry out an experiment there is usually an expectation that the result will fall within a range of...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/B:PRES.0000030439.62331.d0
更新日期：2004-01-01 00:00:00
abstract：:Biological water oxidation, performed by a single enzyme, photosystem II, is a central research topic not only in understanding the photosynthetic apparatus but also for the development of water splitting catalysts for technological applications. Great progress has been made in this endeavor following the report of a ...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-019-00648-3
更新日期：2019-10-01 00:00:00
abstract：:Bill Arnold's concept of photosynthesis as a solid state, as opposed to solution, phenomenon led him to an amazing series of fundamental discoveries. ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00040990
更新日期：1996-05-01 00:00:00
abstract：:Cassava, bean and maize leaves were fed with(14)CO2 in light and the primary products of photosynthesis identified 5 and 10 seconds after assimilation. In maize, approximately three quarters of the labelled carbon was incorporated in C4 acids, in beans about two thirds in PGA, and in cassava approximately 40-60% in C4...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00055123
更新日期：1987-01-01 00:00:00
abstract：:The dependence on temperature of tryptophan fluorescence lifetime in trimeric photosystem I (PSI) complexes from cyanobacteria Synechocystis sp. PCC 6803 during the heating of pre-frozen to - 180 °C in the dark or in the light-activated preparations has been studied. Fluorescence lifetime in samples frozen in the ligh...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-018-0595-8
更新日期：2019-03-01 00:00:00
abstract：:The effect of light on the metabolism of ammonia was studied by subjecting detached maize leaves to 150 or 1350 μmol m(-2) s(-1) PAR during incubation with the leaf base in 2 mM (15)NH4Cl. After up to 60 min, leaves were extracted. Ammonia, glutamine, glycine, serine, alanine, and aspartate were separated by isotherma...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00049278
更新日期：1985-09-01 00:00:00
abstract：:We present a theoretical study of excitation dynamics in the chlorosome antenna complex of green photosynthetic bacteria based on a recently proposed model for the molecular assembly. Our model for the excitation energy transfer (EET) throughout the antenna combines a stochastic time propagation of the excitonic wave ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-014-9978-7
更新日期：2014-06-01 00:00:00
abstract：:Green sulfur bacteria and heliobacteria are strictly anaerobic phototrophs that have homodimeric type 1 reaction center complexes. Within these complexes, highly reducing substances are produced through an initial charge separation followed by electron transfer reactions driven by light energy absorption. In order to ...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-009-9521-4
更新日期：2010-06-01 00:00:00
abstract：:Bioenergetic properties of thylakoids from plants submitted to a water stress stress (watering stopped for 6-15 days) have been measured in two lupin genotypes characterized as resistant or susceptible to drought. This energy coupling was assessed by flow-force relationships relating the phosphorylation rate to the ma...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00035944
更新日期：1992-05-01 00:00:00
abstract：:Although it has been believed that wild-type plants are capable of protecting photosystem I (PSI) under high light, our previous study indicates that PSI is sensitive to high light in the shade-established tree species Psychotria rubra. However, the underlying physiological mechanisms are unclear. In this study, we ex...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-016-0275-5
更新日期：2016-07-01 00:00:00
abstract：:A proton electrochemical potential across the membranes of photosynthetic purple bacteria is established by a light-driven proton pump mechanism: the absorbed light in the reaction center initiates electron transfer which is coupled to the vectorial displacement of protons from the cytoplasm to the periplasm. The stoi...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF02185435
更新日期：1993-07-01 00:00:00
