Abstract：
:Earlier mass spectrometric measurements, in which oxygen evolution was measured following short saturating light flashes, indicated that with a time resolution of about 30 s no form of bound water and/or an oxidation product exists up to the redox state S3 of the oxygen evolving center (R. Radmer and O. Ollinger, 1986, FEBS Lett 195: 285-289; K.P. Bader, P. Thibault and G.H. Schmid, 1987, Biochim Biophys Acta 893: 564-571). In the present study, isotope exchange experiments with H2 (18)O were performed under different experimental conditions. We found: a) the isotope exchange pattern is virtually the same at both pH 6.0 and 7.8, although marked structural changes of the PS II donor side are inferred to take place within this pH-range (Renger G., Messinger J. and Wacker U., 1992, Research in Photosynthesis, II: 329-332); b) injection of H2 (18)O at about 0°C gives rise to mass ratios of the evolved oxygen which markedly deviate from the theoretically expected values of complete isotope scrambling; and c) rapid injection of H2 (18)O into samples with high population of S1 and S2 and subsequent illumination with three and two flashes, respectively, spaced by a dark time of only 1.5 ms lead to similar (18)O-labeling of the evolved oxygen. Based on the published data on the interaction with redox active amines, possible pathways of substrate exchange in the water oxidase are discussed.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Bader KP,Renger G,Schmid GHdoi
10.1007/BF00046761subject
Has Abstractpub_date
1993-01-01 00:00:00pages
355-61issue
3eissn
0166-8595issn
1573-5079journal_volume
38pub_type
杂志文章abstract：:We have compared the properties of a mutant of barley lacking Photosystem I (viridis-zb (63) ) with the corresponding wild type using modulated fluorescence measurements. The mutant showed two unexpected characteristics. Firstly, there was a slow decline in the fluorescence signal in the light which was dependent on t...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00016561
更新日期：1993-03-01 00:00:00
abstract：:The current status of research on the structure, regulation, mechanism and importance of Rubisco activase is reviewed. The activase is now recognized to be a member of the AAA(+) family, whose members participate in macromolecular complexes that perform diverse chaperone-like functions. The conserved nucleotide-bindin...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1022458108678
更新日期：2003-01-01 00:00:00
abstract：:Treatment of lyophilized thylakoid membranes of the thermophilic cyanobacterium Synechococcus sp. with n-heptane for 6 h resulted in marked changes in the pattern of photosystem I reaction center complexes resolved by sodium dodecylsulfate-polyacrylamide gel electrophoresis. CP1-a, which consists of two large subunits...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00117671
更新日期：1987-01-01 00:00:00
abstract：:Rhodobacter sphaeroides 2.4.1 is an alpha-3 purple nonsulfur eubacterium with an extensive metabolic repertoire. Under anaerobic conditions, it is able to grow by photosynthesis, respiration and fermentation. Photosynthesis may be photoheterotrophic using organic compounds as both a carbon and a reducing source, or ph...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1013831823701
更新日期：2001-01-01 00:00:00
abstract：:Photosynthetic organisms normally endeavor to optimize the efficiency of their light-harvesting apparatus. However, here we describe two bacterial isolates belonging to the genera Allochromatium and Thiocapsa that demonstrate a novel adaptation by optimizing their external growth conditions at the expense of photosynt...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-007-9246-1
更新日期：2008-02-01 00:00:00
abstract：:The Mg-dechelating activity of extracts of Chenopodium album (goosefoot) was investigated using an artificial substrate, chlorophyllin a. The activity was measured spectrophotometrically by the formation of a reaction product, pheophorbin a (Mg-free chlorin), after release of the central Mg. The Mg-releasing protein w...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1020915812770
更新日期：2002-01-01 00:00:00
abstract：:The problem of predicting the kinetics of proton efflux and the decay of the internal proton concentration for vesicles containing one or more buffers for which the internal proton concentration is initially higher than that of the surrounding medium is examined. An analytical solution is derived that describes the ti...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00019150
更新日期：1987-01-01 00:00:00
abstract：:Photosynthesis powers our planet and is a source of inspiration for developing artificial constructs mimicking many aspects of the natural energy transducing process. In the complex machinery of photosystem II (PSII), the redox activity of the tyrosine Z (Tyrz) hydrogen-bonded to histidine 190 (His190) is essential fo...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-020-00815-x
更新日期：2021-01-11 00:00:00
abstract：:In thylakoid membranes, several peptides of high MW† are present which may interfere with the study of CP1's components. Modifying Cleveland's technique [7] for limited proteolysis, we have characterized the polypeptides found in the 60 kD region. Some may result from incomplete washing of the CF1 while others come fr...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00020071
更新日期：1980-03-01 00:00:00
abstract：:Application of target theory to the photoinactivation of Photosystem II in pea leaf discs (Park et al. 1995, 1996a,b) reveals that there is a critical light dosage below which there is complete photoprotection and above which there is photoinactivation (i.e a light-induced loss of oxygen flash yield). The critical dos...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00018219
更新日期：1996-10-01 00:00:00
abstract：:Flash-induced absorbance measurements at 830 nm on both nanosecond and microsecond timescales have been used to characterise the effect of ultraviolet light on Photosystem II core particles. A combination of UV-A and UV-B, closely simulating the spectrum of sunlight below 350 nm, was found to have a primary effect on ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00029424
更新日期：1996-07-01 00:00:00
abstract：:Redox dependent protein phosphorylation in chloroplast thylakoids regulates distribution of excitation energy between the two photosystems of photosynthesis, PS I and PS II. Several thylakoid phosphoproteins are known to be phosphorylated on N-terminal threonine residues exposed to the chloroplast stroma. Phosphorylat...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1011891017067
更新日期：2001-01-01 00:00:00
abstract：:Species in the Laxa and Grandia groups of the genus Panicum are adapted to low, wet areas of tropical and subtropical America. Panicum milioides is a species with C3 photosynthesis and low apparent photorespiration and has been classified as a C3/C4 intermediate. Other species in the Laxa group are C3 with normal phot...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00024189
更新日期：1986-01-01 00:00:00
abstract：:Data on cadmium accumulation in chloroplasts of terrestrial plants are scarce and contradictory. We introduced CdSO4 in hydroponic media to the final concentrations 80 and 250 μM and studied the accumulation of Cd in chloroplasts of Hordeum vulgare and Zea mays. Barley accumulated more Cd in the chloroplasts as compar...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-014-0047-z
更新日期：2015-08-01 00:00:00
abstract：:Recent magnetic-resonance work on YŻ suggests that this species exhibits considerable motional flexibility in its functional site and that its phenol oxygen is not involved in a well-ordered hydrogen-bond interaction (Tang et al., submitted; Tommos et al., in press). Both of these observations are inconsistent with a ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00020428
更新日期：1995-11-01 00:00:00
abstract：:The role of photosynthetic pigments in the development of separation methods in biochemistry during the period 1900-1980 is described beginning with M. Tswett who introduced separation of chlorophylls and carotenoids on columns and coined the term chromatography in 1906. In Uppsala, T. Svedberg developed the ultracent...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1024944606930
更新日期：2003-01-01 00:00:00
abstract：:An immunological method for quantitative determination of photosynthetic fructose-1,6-bisphosphatase in crude extracts of leaves is proposed. It is based on the ELISA technique, and offers two modifications. A non-competitive technique has a higher sensitivity and is the right option for samples of low fructose-1,6-bi...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00032710
更新日期：1987-01-01 00:00:00
abstract：:Sulphoquinovosyldiacylglycerol is a negatively charged lipid which exists in the thylakoid membrane. It is proposed that a large proportion of this acidic lipid does not form a part of the bulk lipid matrix but is closely associated with protein complexes where it is tightly bound and participates in either optimising...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00029747
更新日期：1986-01-01 00:00:00
abstract：:We present here our tribute to an outstanding unique scientist, a pioneer of plant biochemistry, best known for his seminal ground-breaking discoveries related to electron and proton transport, and their intimate relation to ATP synthesis in chloroplasts. His dedication and in-depth understanding of science was matche...
journal_title：Photosynthesis research
pub_type： 传,历史文章,杂志文章
doi：10.1007/s11120-017-0380-0
更新日期：2017-06-01 00:00:00
abstract：:To investigate the impact of iron deficiency on bioenergetic pathways in Chlamydomonas, we compared growth rates, iron content, and photosynthetic parameters systematically in acetate versus CO(2)-grown cells. Acetate-grown cells have, predictably (2-fold) greater abundance of respiration components but also, counter-...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-010-9562-8
更新日期：2010-07-01 00:00:00
abstract：:Leaf variegation mutants constitute a unique group of chloroplast development mutants and are ideal genetic materials to dissect the regulation of chloroplast development. We have utilized the Arabidopsis yellow variegated (var2) mutant and genetic suppressor analysis to probe the mechanisms of chloroplast development...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-015-0195-9
更新日期：2016-03-01 00:00:00
abstract：:Cyanobacteria perform photosynthesis with the use of large light-harvesting antennae called phycobilisomes (PBSs). These hemispherical PBSs contain hundreds of open-chain tetrapyrrole chromophores bound to different peptides, providing an arrangement in which excitation energy is funnelled towards the PBS core from wh...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-017-0417-4
更新日期：2018-03-01 00:00:00
abstract：:Rates of net CO2 uptake were examined in developing leaves of Hydrocotyle bonariensis. Leaves that developed under high photosynthetically active radiation (48 mol m(-2) day(-1) PAR) were smaller, thicker, and reached maximum size sooner than did leaves that developed under low PAR (4.8 mol m(-2) day(-1)). Maximum net...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00028750
更新日期：1981-06-01 00:00:00
abstract：:A pigment system containing carotenoids and oxidised reaction centre pigments is present in chromatophores of Rhodospirillum rubrum and this pigment system may cause fluorescence quenching when a still unidentified chromatophore component is in its oxidised state. Besides by its action spectrum, this pigment system is...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00018376
更新日期：1984-03-01 00:00:00
abstract：:It is already well established that the high-frequency intramolecular vibrations are responsible for many observed dynamic phenomena in linear and nonlinear electronic spectroscopy such as the spectral lineshape formation, the transition dipole moment, the lifetime borrowing, and vibrational and mixed coherence beats....
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-014-0002-z
更新日期：2014-07-01 00:00:00
abstract：:DCMU (3-(3,4-dichlorophenyl)-1,1-dimethylurea) at concentrations higher than 10 μM suppresses the second time range delayed fluorescence (DF) of pea chloroplasts, due to inhibition of the oxidizing side of photosystem II (PS II). The inhibition of the reducing side of PS II resulting in the suppression of millisecond ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00054986
更新日期：1988-01-01 00:00:00
abstract：:The availability of a complete genome database for the cyanobacterium Synechocystis sp. PCC6803 (glucose-tolerant strain) has raised expectations that this organism would become a reference strain for work aimed at understanding the CO(2)-concentrating mechanism (CCM) in cyanobacteria. However, the amount of physiolog...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1025850230977
更新日期：2003-01-01 00:00:00
abstract：:A correlated influence of cation concentration and excitation energy level on PS II Abbreviations: AMPD = 2-amino-2-methyl-1,3-propanediol; DCIP = 2-6 dichlorophenol indophenol; DCIPH2 = DCIP in reduced state; DCIPr = rate of DCIP-reduction; DCMU = 3-(3,4 dichlorophenyl)1-1 dimethyl-urea; Fv = variable fluorescence; F...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00018228
更新日期：1980-06-01 00:00:00
abstract：:C3 carbon fixation has a bad reputation, primarily because it is associated with photorespiration, a biochemical pathway thought to waste a substantial amount of the carbohydrate produced in a plant. This review presents evidence collected over nearly a century that (1) Rubisco when associated with Mn(2+) generates ad...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-014-0056-y
更新日期：2015-02-01 00:00:00
abstract：:A recent publication (Esteban in New Phytol 217:341-342, 2018) describes how the use and citation of the assay of chlorophylls a and b extracted in aqueous 80% acetone by Arnon (Plant Physiol 2:1-15, 1949) is increasing, even in journals with high impact factors. This is a very disconcerting situation: the assay is ou...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-018-0579-8
更新日期：2019-05-01 00:00:00
