Abstract：
:Application of target theory to the photoinactivation of Photosystem II in pea leaf discs (Park et al. 1995, 1996a,b) reveals that there is a critical light dosage below which there is complete photoprotection and above which there is photoinactivation (i.e a light-induced loss of oxygen flash yield). The critical dosage is about 3 mol photons m(-2) for medium and high light-grown leaves and 0.36 mol photons m(-2) for low light-grown leaves. Photoinactivation is a one-hit process with an effective cross-section of 0.045 m(2) mol(-1) photons which does not vary with growth irradiance, unlike the cross-section for oxygen evolution which increases with decreasing growth irradiance. The cross-section for oxygen evolution increased by about 20% following exposure to 6.8 mol photons m(-2) which may be due to energy transfer from photoinactivated units to functional Photosystem II units. We propose that the photoinactivation of PS II begins when a small group of PS II pigment molecules whose structure is uninfluenced by growth irradiance, becomes uncoupled energetically from the rest of the photosynthetic unit and thus no longer transfers excitions to P680. De-excitation of this group of pigment molecules provides the energy which leads to the damage of Photosystem II. Treatment of pea leaves with dithiothreitol, an inhibitor of the xanthophyll cycle, decreases the critical dosage i.e. decreases photoprotection but has no effect on the PS II photoinactivation cross-section. Treatment with 1 μM nigericin increased the photoinactivation cross-section of PS II as did exposure to lincomycin which inhibits D1 protein synthesis and thus the repair of PS II reaction centres.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Sinclair J,Park YI,Chow WS,Anderson JMdoi
10.1007/BF00018219subject
Has Abstractpub_date
1996-10-01 00:00:00pages
33-40issue
1eissn
0166-8595issn
1573-5079journal_volume
50pub_type
杂志文章abstract：:A protocol for the isolation of functional thylakoids from Arabidopsis thaliana leaves was developed. The critical factor in obtaining active, coupled and stable preparation is the inclusion of EDTA and EGTA in the grinding buffer. Preparations were characterized with respect to the whole or partial electron transport...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1011818021875
更新日期：2001-01-01 00:00:00
abstract：:The authors present a study of the fluorescence and absorbance transients occurring in whole cells of purple nonsulfur bacterium Rhodobacter sphaeroides on the millisecond timescale under pulsed actinic illumination. The fluorescence induction curve is interpreted in terms of combination of effects of redox changes in...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-010-9569-1
更新日期：2010-08-01 00:00:00
abstract：:Exposure of the red alga Porphyra perforata or leaves of Phytolacca americana and Echinodorus sp. to white light equivalent to full sunlight for short periods induced large decreases of variable fluorescence measured at 695 nm at 77K. This change was not produced by photoinhibition but rather appeared to result from a...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00118298
更新日期：1986-01-01 00:00:00
abstract：:The world is facing energy crisis and environmental issues due to the depletion of fossil fuels and increasing CO2 concentration in the atmosphere. Growing microalgae can contribute to practical solutions for these global problems because they can harvest solar energy and capture CO2 by converting it into biofuel usin...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-013-9828-z
更新日期：2013-11-01 00:00:00
abstract：:The genome of the purple non-sulphur photosynthetic bacterium Rhodopseudomonas acidophila has been found to contain multiple copies of puc light-harvesting (LH2) peripheral antenna complex genes. Three wild-type isolates each exhibiting dissimilar peripheral antenna complex phenotypes in response to growth at reduced ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00034783
更新日期：1996-09-01 00:00:00
abstract：:Phosphatidylglycerol (PG), containing the unique fatty acid Delta3, trans-16:1-hexadecenoic acid, is a minor but ubiquitous lipid component of thylakoid membranes of chloroplasts and cyanobacteria. We investigated its role in electron transfers and structural organization of Photosystem II (PSII) by treating Arabidops...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-006-9126-0
更新日期：2007-07-01 00:00:00
abstract：:In this article I review the history of research on the Fenna-Matthews-Olson (FMO) protein with emphasis on my contributions. The FMO protein, which transfers energy from the chlorosome to the reaction center in green sulfur bacteria, was discovered in 1962 and shown to contain bacteriochlorophyll a. From the absorpti...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/B:PRES.0000030428.36950.43
更新日期：2004-01-01 00:00:00
abstract：:The thylakoid membrane protein PsbS is critical for quenching excessive excitation energy in mechanisms that involve the light-harvesting complexes of photosystem II. Liposomes of thylakoid lipids have been shown to be a very good platform to study photosynthetic membrane proteins and their interactions. In this study...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-015-0176-z
更新日期：2016-01-01 00:00:00
abstract：:Effect of chemical oxidation by ferricyanide on bacteriochlorophyll a (BChl a) in the Fenna-Matthews-Olson protein (FMO) was studied using absorbance and fluorescence spectroscopy at ambient and cryogenic temperatures. Partially selective oxidation of pigments bound to the antenna complex was achieved and the probable...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-013-9878-2
更新日期：2013-09-01 00:00:00
abstract：:The binding characteristics and the inhibitory power of atrazine and DCMU towards uncoupled electron flow activity were studied in acyl lipid-depleted thylakoid membranes from atrazine-susceptible and-resistant biotypes of Solanum nigrum L. For this purpose, phospholipase A2 from Vipera russelli and the lipase from Rh...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00049537
更新日期：1992-01-01 00:00:00
abstract：:Treatment of lyophilized thylakoid membranes of the thermophilic cyanobacterium Synechococcus sp. with n-heptane for 6 h resulted in marked changes in the pattern of photosystem I reaction center complexes resolved by sodium dodecylsulfate-polyacrylamide gel electrophoresis. CP1-a, which consists of two large subunits...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00117671
更新日期：1987-01-01 00:00:00
abstract：:Recently, a number of techniques, some of them relatively new and many often used in combination, have given a clearer picture of the dynamic role of electron transport in Photosystem I of photosynthesis and of coupled cyclic photophosphorylation. For example, the photoacoustic technique has detected cyclic electron t...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00033035
更新日期：1993-06-01 00:00:00
abstract：:Characteristics of primary phases in chlorophyll a fluorescence transients based on room temperature in vivo measurement with a Plant Productivity Fluorometer (Brancker Model SF-10) can be greatly facilitated by coupling the instrument to a fast data acquisition system. The SF-10 was linked to a Multitech Industrial C...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00041817
更新日期：1983-01-01 00:00:00
abstract：:The relationship between the redox state of P700, the primary donor of PS I, monitored using absorbance changes at 830 nm and photochemical energy storage in PS I reaction centers assayed with the photoacoustic method (PA) was studied in isolated PS I submembrane particles aspirated onto nitrocellulose filters. Severa...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1021237026500
更新日期：2002-01-01 00:00:00
abstract：:After a brief prologue on Otto Kandler's life, we describe briefly his pioneering work on photosynthesis (photophosphorylation and the carbon cycle) and his key participation in the discovery of the concept of three forms of life (Archaea, Prokarya, and Eukarya). With Otto Kandler's passing, both the international pho...
journal_title：Photosynthesis research
pub_type： 传,历史文章,杂志文章
doi：10.1007/s11120-018-0530-z
更新日期：2018-09-01 00:00:00
abstract：:Ferredoxin and the flavoprotein, ferredoxin: NADP reductase, have been covalently linked by incubation in the presence of a water soluble carbodiimide. The cross-linking reaction yields an adduct having a 1:1 stoichiometry. The adduct has depressed levels of diaphorase and NADPH oxidase activity and is inactive in red...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00035450
更新日期：1988-09-01 00:00:00
abstract：:Chlorophyll f is a photosynthetic pigment that was discovered in 2010. In this study, we present investigations on spectral and dynamic characteristics of singlet-excited and triplet states of Chl f with the application of ultrafast time-resolved absorption and fluorescence spectroscopies. The pigment was studied at r...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-014-9981-z
更新日期：2014-07-01 00:00:00
abstract：:It had been proposed that a loop, typically containing 26 or 27 amino acids, which is only present in monomeric, ferredoxin-dependent, "plant-type" glutamate synthases and is absent from the catalytic α-subunits of both NADPH-dependent, heterodimeric glutamate synthases found in non-photosynthetic bacteria and NADH-de...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-014-0044-2
更新日期：2015-02-01 00:00:00
abstract：:Light energy harvested by the pigments in Photosystem I (PSI) is used for charge separation in the reaction center (RC), after which the positive charge resides on a special chlorophyll dimer called P700. In studies on the PSI trapping kinetics, P700(+) is usually chemically reduced to re-open the RCs. So far, the inf...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-011-9671-z
更新日期：2012-03-01 00:00:00
abstract：:Minimal photosynthetic catalytic F1(αβ) core complexes, containing equimolar ratios of the α and β subunits, were isolated from membrane-bound spinach chloroplast CF1 and Rhodospirillum rubrum chromatophore RrF1. A CF1-α3β3 hexamer and RrF1-α1β1 dimer, which were purified from the respective F1(αβ) complexes, exhibit ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00020418
更新日期：1995-11-01 00:00:00
abstract：:Tropical plants are sensitive to chilling temperatures above zero but it is still unclear whether photosystem I (PSI) or photosystem II (PSII) of tropical plants is mainly affected by chilling temperatures. In this study, the effect of 4 degrees C associated with various light densities on PSII and PSI was studied in ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-010-9539-7
更新日期：2010-03-01 00:00:00
abstract：:The oxidation kinetics under far-red light (FRL) of photosystem I (PSI) high potential donors P700, plastocyanin (PC), and cytochrome f (Cyt f) were investigated in sunflower leaves with the help of a new high-sensitivity photometer at 810 nm. The slopes of the 810 nm signal were measured immediately before and after ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-007-9224-7
更新日期：2007-10-01 00:00:00
abstract：:Dry weight and Relative Growth Rate of Lemna gibba were significantly increased by CO2 enrichment up to 6000 μl CO2 l(-1). This high CO2 optimum for growth is probably due to the presence of nonfunctional stomata. The response to high CO2 was less or absent following four days growth in 2% O2. The Leaf Area Ratio decr...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00029048
更新日期：1985-03-01 00:00:00
abstract：:Chlorophyll (Chl) b serves an essential function in accumulation of light-harvesting complexes (LHCs) in plants. In this article, this role of Chl b is explored by considering the properties of Chls and the ligands with which they interact in the complexes. The overall properties of the Chls, not only their spectral f...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-007-9181-1
更新日期：2007-11-01 00:00:00
abstract：:Precise control of the proton concentration gradient across thylakoid membranes (ΔpH) is essential for photosynthesis and its regulation because the gradient contributes to the generation of the proton motive force used for ATP synthesis and also for the fast and reversible induction of non-photochemical quenching (NP...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-018-0575-z
更新日期：2019-03-01 00:00:00
abstract：:In higher plants, photosystem II (PSII) is a multi-subunit pigment-protein complex embedded in the thylakoid membranes of chloroplasts, where it is present mostly in dimeric form within the grana. Its light-harvesting antenna system, LHCII, is composed of trimeric and monomeric complexes, which can associate in variab...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-016-0216-3
更新日期：2016-12-01 00:00:00
abstract：:The chlorophyll content is an important experimental parameter in agronomy and plant biology research. In this report, we explore the feasibility of determining total concentration of extracts containing chlorophyll a and chlorophyll b by chlorophyll fluorescence. We found that an excitation at 457 nm results in the s...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-012-9777-y
更新日期：2012-10-01 00:00:00
abstract：:The factors governing quinone protein binding in the photosynthetic bacterium Rps. viridis are investigated via use of empirical intermolecular energy calculations using the GRID method (PJ Goodford (1985) J Med Chem 28: 849-854). A probe atom corresponding to a carbonyl oxygen atom is used to investigate regions exhi...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00027142
更新日期：1994-01-01 00:00:00
abstract：:The light response curves of the acceptor and donor side mechanisms of photoinhibition of Photosystem II were calculated, using Arabidopsis as a model organism. Acceptor-side photoinhibition was modelled as double reduction of QA, noting that non-photochemical quenching has the same effect on the quantum yield of QA d...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-004-7174-x
更新日期：2005-06-01 00:00:00
abstract：:Photoacclimation by strains of Haslea "blue" diatom species H. ostrearia and H. silbo sp. nov. ined. was investigated with rapid light curves and induction-recovery curves using fast repetition rate fluorescence. Cultures were grown to exponential phase under 50 µmol m-2 s-1 photosynthetic available radiation (PAR) an...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-018-0508-x
更新日期：2018-09-01 00:00:00
