Abstract：
:It had been proposed that a loop, typically containing 26 or 27 amino acids, which is only present in monomeric, ferredoxin-dependent, "plant-type" glutamate synthases and is absent from the catalytic α-subunits of both NADPH-dependent, heterodimeric glutamate synthases found in non-photosynthetic bacteria and NADH-dependent heterodimeric cyanobacterial glutamate synthases, plays a key role in productive binding of ferredoxin to the plant-type enzymes. Site-directed mutagenesis has been used to delete the entire 27 amino acid-long loop in the ferredoxin-dependent glutamate synthase from the cyanobacterium Synechocystis sp. PCC 6803. The specific activity of the resulting loopless variant of this glutamate synthase, when reduced ferredoxin serves as the electron donor, is actually higher than that of the wild-type enzyme, suggesting that this loop is not absolutely essential for efficient electron transfer from reduced ferredoxin to the enzyme. These results are consistent with the results of an in-silico study that suggests that the loop is unlikely to interact directly with ferredoxin in the energetically most favorable model of a 1:1 complex of ferredoxin with the wild-type enzyme.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Tripathy JN,Hirasawa M,Sutton RB,Dasgupta A,Vaidyanathan N,Zabet-Moghaddam M,Florencio FJ,Srivastava AP,Knaff DBdoi
10.1007/s11120-014-0044-2subject
Has Abstractpub_date
2015-02-01 00:00:00pages
129-39issue
2eissn
0166-8595issn
1573-5079journal_volume
123pub_type
杂志文章abstract：:MOA-stilbene is known to be a specific inhibitor of the Qo site of mammalian cytochrome bc 1 complex. We show that it also binds to the chloroplast cytochrome bf complex. Binding to the reduced enzyme induces a red-shift of the Soret and visible absorption bands of the haems b. Steady state and single turnover experim...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00029819
更新日期：1992-12-01 00:00:00
abstract：:C3 carbon fixation has a bad reputation, primarily because it is associated with photorespiration, a biochemical pathway thought to waste a substantial amount of the carbohydrate produced in a plant. This review presents evidence collected over nearly a century that (1) Rubisco when associated with Mn(2+) generates ad...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-014-0056-y
更新日期：2015-02-01 00:00:00
abstract：:Energy transfer of the light harvesting complex LHC-II trimer, extracted from spinach, was studied in the Q(y) region at room temperature by femtosecond transient absorption spectroscopy. Configuration interaction exciton method [Linnanto et al. (1999) J Phys Chem B 103: 8739-8750] and 2.72 A structural information re...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-005-9004-1
更新日期：2006-03-01 00:00:00
abstract：:Solubilisation of thylakoid membranes from young leaves of Pisum sativum in the presence of Triton X-100 resulted in an almost complete loss of quenching of light-harvesting chlorophyll-protein (LHCP) fluorescence, as measured at 77°K. There were concomitant changes in the kinetics of light-saturation curves of electr...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00037130
更新日期：1986-01-01 00:00:00
abstract：:NADP:ferredoxin oxidoreductase (EC. 1.6.7.1.) isolated from a thermophilic blue-green alga, Synechococcus sp., was stable at temperatures up to 65°C. The diaphorase and cytochrome c reductase activities of the enzyme were low at 25°C but increased with elevated temperature to reach a maximum at about 60°C. The pH-prof...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00020595
更新日期：1980-09-01 00:00:00
abstract：:In this communication, evidence is presented from the kinetics of QA (-) decay (where QA is the first plastoquinone electron acceptor of photosystem II) and oxygen evolution for the requirement of bicarbonate in the electron transport in a cyanobacteriumSynechocystis (Pasteur Culture Collection 6803). A large slowing ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00046879
更新日期：1988-10-01 00:00:00
abstract：:Phosphatidylglycerol (PG), containing the unique fatty acid Delta3, trans-16:1-hexadecenoic acid, is a minor but ubiquitous lipid component of thylakoid membranes of chloroplasts and cyanobacteria. We investigated its role in electron transfers and structural organization of Photosystem II (PSII) by treating Arabidops...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-006-9126-0
更新日期：2007-07-01 00:00:00
abstract：:Peridinin-chlorophyll-protein (PCP), containing differently absorbing chlorophyll derivatives, are good models with which to study energy transfer among monomeric chlorophylls (Chls) by both bulk and single-molecule spectroscopy. They can be obtained by reconstituting the N-terminal domain of the protein (N-PCP) with ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-007-9277-7
更新日期：2008-02-01 00:00:00
abstract：:Relationships between induced high leaf intercellular CO₂ concentrations, leaf K⁺ and NO₃⁻ ion movement and early fruit formation under macronutrient limitation are not well understood. We examined the effects and interactions of reduced K/N input treatments on leaf intercellular CO₂, photosynthesis rate, carboxylatio...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-013-9832-3
更新日期：2013-07-01 00:00:00
abstract：:The binding characteristics and the inhibitory power of atrazine and DCMU towards uncoupled electron flow activity were studied in acyl lipid-depleted thylakoid membranes from atrazine-susceptible and-resistant biotypes of Solanum nigrum L. For this purpose, phospholipase A2 from Vipera russelli and the lipase from Rh...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00049537
更新日期：1992-01-01 00:00:00
abstract：:Two portable instruments, designed to evaluate epidermal UV screening in leaves, were compared: the Dualex and the UV-A-PAM fluorimeter. Both instruments excite chlorophyll fluorescence at the same UV wavelengths but reference excitation is in the red and the blue spectral range in the former and the latter fluorimete...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-007-9135-7
更新日期：2007-07-01 00:00:00
abstract：:The relationship between the redox state of P700, the primary donor of PS I, monitored using absorbance changes at 830 nm and photochemical energy storage in PS I reaction centers assayed with the photoacoustic method (PA) was studied in isolated PS I submembrane particles aspirated onto nitrocellulose filters. Severa...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1021237026500
更新日期：2002-01-01 00:00:00
abstract：:We present the wavelength dependence of homogeneous holewidths of persistent spectral holes burnt in O2-evolving Photosystem II core complexes isolated from spinach, in the temperature range 2.5-8 K. The data supports the assignment that those chlorophylls which undergo persistent spectral hole-burning are specific CP...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-004-7927-6
更新日期：2005-06-01 00:00:00
abstract：:Around 1960 experiments of Arnold and Clayton, Chance and Nishimura and Calvin and coworkers demonstrated that the primary photosynthetic electron transfer processes are not abolished by cooling to cryogenic temperatures. After a brief historical introduction, this review discusses some aspects of electron transfer in...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00041000
更新日期：1996-05-01 00:00:00
abstract：:Data on cadmium accumulation in chloroplasts of terrestrial plants are scarce and contradictory. We introduced CdSO4 in hydroponic media to the final concentrations 80 and 250 μM and studied the accumulation of Cd in chloroplasts of Hordeum vulgare and Zea mays. Barley accumulated more Cd in the chloroplasts as compar...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-014-0047-z
更新日期：2015-08-01 00:00:00
abstract：:Modulation of the efficiency with which leaves convert absorbed light to photochemical energy [intrinsic efficiency of open photosystem II (PSII) centers, as the ratio of variable to maximal chlorophyll fluorescence] as well as leaf xanthophyll composition (interconversions of the xanthophyll cycle pigments violaxanth...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-012-9761-6
更新日期：2012-09-01 00:00:00
abstract：:Several protein-coding genes from land plant chloroplasts have been shown to contain introns. The majority of these introns resemble the fungal mitochondrial group II introns due to considerable nucleotide sequence homology at their 5' and 3' ends and they can readily be folded to form six hairpins characteristic of t...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00039484
更新日期：1988-04-01 00:00:00
abstract：:The authors present a study of the fluorescence and absorbance transients occurring in whole cells of purple nonsulfur bacterium Rhodobacter sphaeroides on the millisecond timescale under pulsed actinic illumination. The fluorescence induction curve is interpreted in terms of combination of effects of redox changes in...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-010-9569-1
更新日期：2010-08-01 00:00:00
abstract：:Earlier mass spectrometric measurements, in which oxygen evolution was measured following short saturating light flashes, indicated that with a time resolution of about 30 s no form of bound water and/or an oxidation product exists up to the redox state S3 of the oxygen evolving center (R. Radmer and O. Ollinger, 1986...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00046761
更新日期：1993-01-01 00:00:00
abstract：:We investigated several photosynthetic parameters of a virescent mutant of durum wheat and of its wild-type. Electron transport rate to ferricyanide was the same in the two genotypes when expressed on leaf area basis while O2 evolution of the leaf tissue in saturating light and CO2 was slightly higher in the yellow ge...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00033365
更新日期：1989-08-01 00:00:00
abstract：:The effects of root applications of kinetin, gibberellic acid (GA3) and indoleacetic acid (IAA) on photosynthesis was measured using an open infrared CO2 gas-exchange system. There was a 30-35% increase in the photosynthetic rates (mg CO2/dm(2)/hr) of attached leaves within 8 hr following root treatment with 0.47 μM k...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00030048
更新日期：1982-01-01 00:00:00
abstract：:The discovery of a new maize (Zea mays L.) transposon system, Mutator, and the cloning of the 1.4 kilobase transposon, Mul, have made feasible the isolation of nuclear photosynthetic genes which are recognized only by their mutant phenotype. Mutant maize plants which express a high chlorophyll fluorescent (hcf) phenot...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00042979
更新日期：1988-10-01 00:00:00
abstract：:The cyanobacterial small CAB-like proteins (SCPs) are one-helix proteins with compelling similarity to the first and third transmembrane helix of proteins belonging to the CAB family of light-harvesting complex proteins in plants. The SCP proteins are transiently expressed at high light intensity and other stress cond...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-007-9244-3
更新日期：2008-02-01 00:00:00
abstract：:Photoinhibition of the light-induced Photosystem I (PS I) electron transfer activity from the reduced dichlorophenol indophenol to methyl viologen was studied. PS I preparations with Chl/P700 ratios of about 180 (PS I-180), 100 (PS I-100) and 40 (PS I(HA)-40) were isolated from spinach thylakoid membranes by the treat...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00016175
更新日期：1996-02-01 00:00:00
abstract：:The Photosystem I reaction centre protein CP1, isolated from barley using polyacrylamide gel electrophoresis showed an EPR (Electron Paramgnetic Resonance) spectrum with the polarisation pattern AEEAAE, typical of the primary donor triplet state (3)P700, created via radical pair formation and recombination. (3)P700 co...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00034795
更新日期：1992-06-01 00:00:00
abstract：:Three Chl-protein complexes were isolated from thylakoid membranes of Bryopsis maxima and Ulva pertusa, marine green algae that inhabit the intertidal zone of the Pacific Ocean off the eastern coast of Japan by dodecyl-beta-D-maltoside polyacrylamide gel electrophoresis. The slowest-moving fractions showed low Chl a/b...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-006-9064-x
更新日期：2006-07-01 00:00:00
abstract：:Pigment-protein-complexes of two chlorophyll b deficient mutants of Arabidopsis and from the wild type were separated electrophoretically. Light-harvesting proteins were absent in the chlorophyll b free mutant ch(1) and their amount was reduced in the mutant ch(2) which has a reduced content of chlorophyll b. The rati...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00041827
更新日期：1983-01-01 00:00:00
abstract：:The first research paper on photosynthesis in China was published by T.T. Li(2) in 1929. Two photosynthesis laboratories were established in Shanghai and Beijing in the 1950s and the 1960s, respectively. A photophosphorylation 'intermediate' was discovered after the energy conversion process was separated into light a...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1024946222187
更新日期：2003-01-01 00:00:00
abstract：:The chloroplast genome of the highly neutral-lipid-producing marine pennate diatom Fistulifera sp. strain JPCC DA0580 was fully sequenced using high-throughput pyrosequencing. The general features and gene content were compared with three other complete diatom chloroplast genomes. The chloroplast genome is 134,918 bp ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-011-9622-8
更新日期：2011-09-01 00:00:00
abstract：:Sulphoquinovosyldiacylglycerol is a negatively charged lipid which exists in the thylakoid membrane. It is proposed that a large proportion of this acidic lipid does not form a part of the bulk lipid matrix but is closely associated with protein complexes where it is tightly bound and participates in either optimising...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00029747
更新日期：1986-01-01 00:00:00
