Abstract：
:In this communication, evidence is presented from the kinetics of QA (-) decay (where QA is the first plastoquinone electron acceptor of photosystem II) and oxygen evolution for the requirement of bicarbonate in the electron transport in a cyanobacteriumSynechocystis (Pasteur Culture Collection 6803). A large slowing down of QA (-) oxidation, measured from the variable chlorophylla fluorescence after saturating actinic flashes, was observed in the thylakoids ofSynechocystis 6803 depleted of bicarbonate in the presence of 25 mM formate. Qualitatively similar results were obtained with DCMU-treated thylakoids. This shows that bicarbonate depletion inhibits electron transport on the acceptor side of photosystem II between QA and the plastoquinone (PQ) pool in cyanobacteria. Addition of 2.5 mM HCO3 (-) fully reversed the inhibition of electron flow caused by bicarbonate depletion. Two exponential phases of QA (-) decay, a fast one and a slow one, were observed with halftimes of approx. 400 μs (fast) and 26 ms (slow) at pH 6.5. At pH 7.5, these phases were approx. 330 μs (fast) and 21 ms (slow), respectively. The amplitude, but not the halftime, of the fast component decreased by about 70% (pH 6.5) or 50% (pH 7.5); this was accompanied by a concomittant increase in the slow phase. Twenty mM bicarbonate stimulated, by a factor of 4, the Hill reaction in bicarbonate-depletedSynechocystis cells. This effect is independent of CO2 fixation as it was observed even in the presence of an inhibitor DBMIB.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Cao J,Govindjeedoi
10.1007/BF00046879subject
Has Abstractpub_date
1988-10-01 00:00:00pages
277-85issue
3eissn
0166-8595issn
1573-5079journal_volume
19pub_type
杂志文章abstract：:The nature of interaction of cytochrome b-559 high potential (HP) with electron transport on the reducing side of photosystem II was investigated by measuring the susceptibility of cytochrome b-559HP to 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) under different conditions. Submicromolar DCMU concentrations decreas...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00035839
更新日期：1991-03-01 00:00:00
abstract：:In recent years the enzymes of the C3 photosynthetic carbon reduction (PCR) cycle have been studied using the techniques of molecular biology. In this review we discuss the primary protein sequences and structural predictions that have been made for a number of these enzymes, which, with the input of crystallographic ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00029971
更新日期：1991-01-01 00:00:00
abstract：:We present a theoretical study of excitation dynamics in the chlorosome antenna complex of green photosynthetic bacteria based on a recently proposed model for the molecular assembly. Our model for the excitation energy transfer (EET) throughout the antenna combines a stochastic time propagation of the excitonic wave ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-014-9978-7
更新日期：2014-06-01 00:00:00
abstract：:The 16th International Congress on Photosynthesis will be held August 11-16, 2013 in St. Louis, Missouri, USA. The congress will include 15 plenary lectures, 21 scientific symposia, poster sessions, exhibitors, opening reception, final banquet, excursions and accompanying persons program. The congress is organized as ...
journal_title：Photosynthesis research
pub_type：
doi：10.1007/s11120-013-9847-9
更新日期：2013-07-01 00:00:00
abstract：:Chlorosomes of green photosynthetic bacterium Chlorobium tepidum contain aggregates of bacteriochlorophyll c (BChl c) with carotenoids and isoprenoid quinones. BChl aggregates with very similar optical properties can be prepared also in vitro either in non-polar solvents or in aqueous buffers with addition of lipids a...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-007-9259-9
更新日期：2008-02-01 00:00:00
abstract：:Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes carboxylation of ribulose-1,5-bisphosphate, the first in a series of reactions leading to the incorporation of atmospheric CO₂ into biomass. Rubisco requires Rubisco activase (RCA), an AAA+ ATPase that reactivates Rubisco by remodelling the conformati...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-013-9964-5
更新日期：2014-03-01 00:00:00
abstract：:Cyanobacteria perform photosynthesis with the use of large light-harvesting antennae called phycobilisomes (PBSs). These hemispherical PBSs contain hundreds of open-chain tetrapyrrole chromophores bound to different peptides, providing an arrangement in which excitation energy is funnelled towards the PBS core from wh...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-017-0417-4
更新日期：2018-03-01 00:00:00
abstract：:The effect of desiccation and rehydration on the function of Photosystem II has been studied in the desiccation tolerant lichen Cladonia convoluta by thermoluminescence. We have shown that in functional fully hydrated thalli thermoluminescence signals can be observed from the recombination of the S2(3)QB (-) (B band),...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00041010
更新日期：1996-05-01 00:00:00
abstract：:Currently, relatively few proteomics studies of chloroplast have been published, but the field has just started emerging and is likely to develop more rapidly in the future. While the complex membrane structure of the chloroplast makes it difficult to study its entire proteome by global approaches, proteomics has cons...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/B:PRES.0000006914.52898.67
更新日期：2003-01-01 00:00:00
abstract：:The problem of predicting the kinetics of proton efflux and the decay of the internal proton concentration for vesicles containing one or more buffers for which the internal proton concentration is initially higher than that of the surrounding medium is examined. An analytical solution is derived that describes the ti...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00019150
更新日期：1987-01-01 00:00:00
abstract：:Time-resolved (TR) infrared (IR) spectroscopy in the nanosecond to second timescale has been extensively used, in the last 30 years, in the study of photosynthetic systems. Interesting results have also been obtained at lower time resolution (minutes or even hours). In this review, we first describe the used technique...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-016-0305-3
更新日期：2017-02-01 00:00:00
abstract：:In vitro studies of the carotenoid peridinin, which is the primary pigment from the peridinin chlorophyll-a protein (PCP) light harvesting complex, showed a strong dependence on the lifetime of the peridinin lowest singlet excited state on solvent polarity. This dependence was attributed to the presence of an intramol...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-005-1447-x
更新日期：2005-11-01 00:00:00
abstract：:Plant terpenoids are among the most diverse group of naturally-occurring organic compounds known, and several are used in contemporary consumer products. Terpene synthase enzymes catalyze complex rearrangements of carbon skeleton precursors to yield thousands of unique chemical structures that range in size from the s...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-014-9979-6
更新日期：2015-03-01 00:00:00
abstract：:The content and composition of pigments and acyl lipids (monogalactosyl diacylglycerol, digalactosyl diacylglycerol and phosphatidyl glycerol) have been investigated in developing chloroplasts isolated from successive 2-cm sections along the leaves of wheat seedlings grown either under 100, 30 or 3 W·m(-2). In all exa...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00035244
更新日期：1986-01-01 00:00:00
abstract：:The quenching of variable fluorescence yield (qN) and the quenching of dark level fluorescence yield (q0) directly atributable to high-energy-state fluorescence quenching (qE) was studied to distinguish between energy dissipation in the antenna and light harvesting complexes (antenna quenching) and energy dissipation ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00033161
更新日期：1990-09-01 00:00:00
abstract：:This contribution gives a brief survey of the short history since the discovery of the aerobic phototrophic bacteria to focus on a general evolutionary scenario. Most of the citations are of reviews that have covered the earlier literature and to which the reader is directed at appropriate places in the following text...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1020493518379
更新日期：2002-01-01 00:00:00
abstract：:In this article I review the history of research on the Fenna-Matthews-Olson (FMO) protein with emphasis on my contributions. The FMO protein, which transfers energy from the chlorosome to the reaction center in green sulfur bacteria, was discovered in 1962 and shown to contain bacteriochlorophyll a. From the absorpti...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/B:PRES.0000030428.36950.43
更新日期：2004-01-01 00:00:00
abstract：:We have previously investigated the response mechanisms of photosystem II complexes from spinach to strong UV and visible irradiations (Wei et al J Photochem Photobiol B 104:118-125, 2011). In this work, we extend our study to the effects of strong light on the unusual cyanobacterium Acaryochloris marina, which is abl...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-013-9946-7
更新日期：2013-11-01 00:00:00
abstract：:Photosynthetic electron transfer has been examined in whole cells, isolated membranes and in partially purified reaction centers (RCs) of Roseicyclus mahoneyensis, strain ML6 and Porphyrobacter meromictius, strain ML31, two species of obligate aerobic anoxygenic phototrophic bacteria. Photochemical activity in strain ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-011-9718-1
更新日期：2012-02-01 00:00:00
abstract：:The rate and extent of light activation of PEPC may be used as another criterion to distinguish C3 and C4 plants. Light stimulated phosphoenolypyruvate carboxylase (PEPC) in leaf discs of C4 plants, the activity being three times greater than that in the dark but stimulation of PEPC was limited about 30% over the dark...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00015061
更新日期：1993-10-01 00:00:00
abstract：:A computer model comprising light reactions in PS II and PS I, electron-proton transport reactions in mesophyll and bundle sheath chloroplasts, all enzymatic reactions and most of the known regulatory functions of NADP-ME type C(4) photosynthesis has been developed as a system of differential budget equations for inte...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1010695402963
更新日期：2000-01-01 00:00:00
abstract：:Humic substances (HSs) can influence the growth and composition of freshwater phytoplankton assemblage. Since HSs contain many phenolic and quinonic moieties and cause growth reductions in eco-physiological field experiments, HSs are considered photosystem II herbicides. To test this specific mode of action in vivo an...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-018-0513-0
更新日期：2018-09-01 00:00:00
abstract：:Inorganic phosphate participates in many fundamental processes within the plant cell. Its broad influence on plant metabolism is related to such key operations as metabolite transport, enzyme regulation and carbohydrate metabolism in general. This review discusses these topics with special emphasis on the role assigne...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00016551
更新日期：1993-03-01 00:00:00
abstract：:Quantum mechanics/molecular mechanics (QM/MM) hybrid methods are currently the most powerful computational tools for studies of structure/function relations and structural refinement of macrobiomolecules (e.g., proteins and nucleic acids). These methods are highly efficient, since they implement quantum chemistry tech...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-009-9467-6
更新日期：2009-11-01 00:00:00
abstract：:The storage of light energy in chemical form through photosynthesis is the key process underlying life as we know it. To utilize photosynthates efficiently as structural materials or as fuel to drive endergonic processes, they have to be transported from where they are produced to where they are needed. In this primer...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-013-9867-5
更新日期：2013-11-01 00:00:00
abstract：:In this paper, we describe a protocol to obtain a site-directed mutants in thepsbA gene ofChlamydomonas reinhardtii, which overcomes several drawbacks of previous protocols, and makes it possible to generate a mutant within a month. Since the large size of the gene, and the presence of four large introns has made mole...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF02187123
更新日期：1994-11-01 00:00:00
abstract：:The engagement of different photoprotective mechanisms in the cells of the carotenogenic astaxanthin-accumulating chlorophyte Haematococcus pluvialis (i) under favorable conditions, (ii) in the course of stress-induced haematocyst formation and (iii) during recovery from the stress was studied. To this end, we followe...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-016-0246-x
更新日期：2016-06-01 00:00:00
abstract：:Here, we describe a new imaging setup able to assess in vivo photosynthetic activity. The system specifically measures time-resolved chlorophyll fluorescence in response to light. It is composed of a fast digital camera equipped with a wide-angle lens for the analysis of samples up to 10 x 10 cm, i.e. entire plants or...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-009-9487-2
更新日期：2009-10-01 00:00:00
abstract：:The chloroplast photo-oxidation and the expression of the Cab gene Lhcb1, encoding the Lhcb1 light-harvesting chlorophyll a/b-protein of PS II, have been studied in leaf cells of maize treated with the two bleaching herbicides norflurazon and amitrole and of the two carotenoid-free mutants vp9 and vp2 grown under high...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1006477215572
更新日期：2000-01-01 00:00:00
abstract：:In thylakoid membranes, several peptides of high MW† are present which may interfere with the study of CP1's components. Modifying Cleveland's technique [7] for limited proteolysis, we have characterized the polypeptides found in the 60 kD region. Some may result from incomplete washing of the CF1 while others come fr...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00020071
更新日期：1980-03-01 00:00:00
