Abstract:
:Peridinin-chlorophyll-protein (PCP), containing differently absorbing chlorophyll derivatives, are good models with which to study energy transfer among monomeric chlorophylls (Chls) by both bulk and single-molecule spectroscopy. They can be obtained by reconstituting the N-terminal domain of the protein (N-PCP) with peridinin and chlorophyll mixtures. Upon dimerization of these "half-mers", homo- and heterochlorophyllous complexes are generated, that correspond structurally to monomeric protomers of native PCP from Amphidinium carterae. Heterochlorophyllous complexes contain two different Chls in the two halves of the complete structure. Here, we report reconstitution of N-PCP with binary mixtures of Chl a, Chl b, and [3-acetyl]-Chl a. The ratios of the pigments were varied in the reconstitution mixture, and relative binding constants were determined from quantification of these pigments in the reconstituted PCPs. We find higher affinities for both Chl b and [3-acetyl]-Chl a than for the native pigment, Chl a.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Brotosudarmo TH,Mackowski S,Hofmann E,Hiller RG,Bräuchle C,Scheer Hdoi
10.1007/s11120-007-9277-7subject
Has Abstractpub_date
2008-02-01 00:00:00pages
247-52issue
2-3eissn
0166-8595issn
1573-5079journal_volume
95pub_type
杂志文章abstract::We have used antibodies generated against synthetic peptides to determine the topology of the 43 kD chlorophyll a binding protein (CP 43) of Photosystem II. Based on the pattern of proteolytic fragments detected (on western blots) by peptide specific antibodies, a six transmembrane span topological model, with the ami...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00019041
更新日期:1994-04-01 00:00:00
abstract::Soybean [Glycine max (L.) Merr. cv. Williams 82 and A3127] plants were grown in the field under long-term soil moisture deficit and irrigation to determine the effects of severe drought stress on the photosynthetic capacity of soybean leaves. Afternoon leaf water potentials, stomatal conductances, intercellular CO2 co...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00051735
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journal_title:Photosynthesis research
pub_type: 杂志文章
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00016274
更新日期:1993-05-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 传,历史文章,杂志文章
doi:10.1007/s11120-012-9752-7
更新日期:2012-09-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00028750
更新日期:1981-06-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF02183048
更新日期:1994-09-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
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更新日期:2014-09-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00118298
更新日期:1986-01-01 00:00:00
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pub_type: 杂志文章
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00016551
更新日期:1993-03-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-018-0567-z
更新日期:2018-12-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1021237026500
更新日期:2002-01-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-007-9266-x
更新日期:2008-02-01 00:00:00
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doi:10.1007/s11120-014-0021-9
更新日期:2014-11-01 00:00:00
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pub_type: 传,历史文章,杂志文章
doi:10.1007/s11120-009-9505-4
更新日期:2010-01-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-008-9291-4
更新日期:2008-05-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00033716
更新日期:1991-05-01 00:00:00
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更新日期:1994-11-01 00:00:00
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更新日期:2002-01-01 00:00:00
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更新日期:2014-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:1994-03-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
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更新日期:1996-01-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
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更新日期:2019-03-01 00:00:00
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更新日期:2016-01-01 00:00:00
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doi:10.1007/s11120-007-9192-y
更新日期:2007-11-01 00:00:00
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pub_type: 杂志文章
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