Abstract：
:Thylakoids are complex sub-organellar membrane systems whose role in photosynthesis makes them critical to life. Thylakoids require the coordinated expression of both nuclear- and plastid-encoded proteins to allow rapid response to changing environmental conditions. Transport of cytoplasmically synthesized proteins to thylakoids or the thylakoid lumen is complex; the process involves transport across up to three membrane systems with routing through three aqueous compartments. Protein transport in thylakoids is accomplished by conserved ancestral prokaryotic plasma membrane translocases containing novel adaptations for the sub-organellar location. This review focuses on the evolutionarily conserved chloroplast twin arginine transport (cpTat) pathway. An overview is provided of known aspects of the cpTat components, energy requirements, and mechanisms with a focus on recent discoveries. Some of the most exciting new studies have been in determining the structural architecture of the membrane complex involved in forming the point of passage for the precursor and binding features of the translocase components. The cpTat system is of particular interest because it transports folded protein domains using only the proton motive force for energy. The implications for mechanism of translocation by recent studies focusing on interactions between membrane Tat components and with the translocating precursor will be discussed.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
New CP,Ma Q,Dabney-Smith Cdoi
10.1007/s11120-018-0567-zsubject
Has Abstractpub_date
2018-12-01 00:00:00pages
289-301issue
3eissn
0166-8595issn
1573-5079pii
10.1007/s11120-018-0567-zjournal_volume
138pub_type
杂志文章,评审abstract：:In studies on photosynthetic systems it is common practice to interpret the results of time-resolved fluorescence experiments on the basis of compartmental, or target, models. Each compartment represents a group of molecules with similar fluorescence characteristics. In cases of practical interest, the members of each...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00014883
更新日期：1996-11-01 00:00:00
abstract：:In this article I review the history of research on the Fenna-Matthews-Olson (FMO) protein with emphasis on my contributions. The FMO protein, which transfers energy from the chlorosome to the reaction center in green sulfur bacteria, was discovered in 1962 and shown to contain bacteriochlorophyll a. From the absorpti...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/B:PRES.0000030428.36950.43
更新日期：2004-01-01 00:00:00
abstract：:The photosystem II (PSII) manganese-stabilizing protein (PsbO) is known to be the essential PSII extrinsic subunit for stabilization and retention of the Mn and Cl(-) cofactors in the oxygen evolving complex (OEC) of PSII, but its function relative to Ca(2+) is less clear. To obtain a better insight into the relations...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-011-9703-8
更新日期：2011-12-01 00:00:00
abstract：:Here, we describe a new imaging setup able to assess in vivo photosynthetic activity. The system specifically measures time-resolved chlorophyll fluorescence in response to light. It is composed of a fast digital camera equipped with a wide-angle lens for the analysis of samples up to 10 x 10 cm, i.e. entire plants or...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-009-9487-2
更新日期：2009-10-01 00:00:00
abstract：:Previous investigations have shown that increased source strength as a result of elevated CO(2) can alter the timing of the phases of change that occur in photosynthetic rates during dicot leaf ontogeny [Miller et al. (1997) Plant Physiol 115: 1195-1200]. To evaluate the converse situation of decreased source strength...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1006367719639
更新日期：2000-01-01 00:00:00
abstract：:Two portable instruments, designed to evaluate epidermal UV screening in leaves, were compared: the Dualex and the UV-A-PAM fluorimeter. Both instruments excite chlorophyll fluorescence at the same UV wavelengths but reference excitation is in the red and the blue spectral range in the former and the latter fluorimete...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-007-9135-7
更新日期：2007-07-01 00:00:00
abstract：:Peridinin-chlorophyll-protein (PCP), containing differently absorbing chlorophyll derivatives, are good models with which to study energy transfer among monomeric chlorophylls (Chls) by both bulk and single-molecule spectroscopy. They can be obtained by reconstituting the N-terminal domain of the protein (N-PCP) with ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-007-9277-7
更新日期：2008-02-01 00:00:00
abstract：:Relationships between induced high leaf intercellular CO₂ concentrations, leaf K⁺ and NO₃⁻ ion movement and early fruit formation under macronutrient limitation are not well understood. We examined the effects and interactions of reduced K/N input treatments on leaf intercellular CO₂, photosynthesis rate, carboxylatio...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-013-9832-3
更新日期：2013-07-01 00:00:00
abstract：:The functional peculiarities and responses of the photosynthetic system in the flowering homoiochlorophyllous desiccation-tolerant (HDT) Haberlea rhodopensis and the non-desiccation-tolerant spinach were compared during desiccation and rehydration. Increasing rate of water loss clearly modifies the kinetic parameters ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-005-2440-0
更新日期：2005-08-01 00:00:00
abstract：:Recycling of carbon by the photorespiratory pathway involves enzymatic steps in the chloroplast, mitochondria, and peroxisomes. Most of these reactions are essential for plants growing under ambient CO(2) concentrations. However, some disruptions of photorespiratory metabolism cause subtle phenotypes in plants grown i...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-011-9651-3
更新日期：2011-09-01 00:00:00
abstract：:With the discovery of targeted gene replacement, moss biology has been rapidly advancing over the last 10 years. This study demonstrates the usefulness of moss as a model organism for plant photosynthesis research. The two mosses examined in this study, Physcomitrella patens and Ceratodon purpureus, are easily culture...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-004-5577-3
更新日期：2005-01-01 00:00:00
abstract：:Chlorophyll (Chl) is an essential component of the photosynthetic apparatus. Embedded into Chl-binding proteins, Chl molecules play a central role in light harvesting and charge separation within the photosystems. It is critical for the photosynthetic cell to not only ensure the synthesis of a sufficient amount of new...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-013-9797-2
更新日期：2014-02-01 00:00:00
abstract：:The interaction of PsaC with the core heterodimer of Photosystem 1 was studied in wild type Synechocystis sp. PCC 6803 and the site-directed mutant R561E of PsaB. The mutant reaction center was much less stable in urea and the functional reconstitution of the mutant core using PsaC was impaired. However, the extent of...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00018261
更新日期：1994-12-01 00:00:00
abstract：:I provide here a personal perspective on (i) the awards that were given to young investigators at the 2008 and 2009 Gordon Research Conferences on Biochemistry/Biophysics of Photosynthesis; and (ii) the ambiance at these conferences through some photographs, particularly of the 2009 conference. ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-009-9479-2
更新日期：2009-10-01 00:00:00
abstract：:Vyacheslav Vasilevich (V.V.) Klimov (or Slava, as most of us called him) was born on January 12, 1945 and passed away on May 9, 2017. He began his scientific career at the Bach Institute of Biochemistry of the USSR Academy of Sciences (Akademy Nauk (AN) SSSR), Moscow, Russia, and then, he was associated with the Insti...
journal_title：Photosynthesis research
pub_type： 传,历史文章,杂志文章
doi：10.1007/s11120-017-0440-5
更新日期：2018-04-01 00:00:00
abstract：:The crystallization of a given protein is a hard task being even more complicated when the protein shows a hydrophobic behavior. In the case of photosynthetic proteins, the difficulty of the experiments increased due to the high light sensitivity. Aqueous solutions of photosystem II core complex (OEC PSII) of Pisum sa...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-007-9131-y
更新日期：2006-12-01 00:00:00
abstract：:A study of the absorption and fluorescence characteristics of the D1/D2/cytb-559 reaction centre complex of Photosystem II has been carried out by gaussian decomposition of absorption spectra both at room temperature and 72 K and of the room temperature fluorescence spectrum. A five component fit was found in which th...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF02183048
更新日期：1994-09-01 00:00:00
abstract：:We evaluate low-temperature (low-T) emission spectra of photosystem II core complexes (PSII-cc) previously reported in the literature, which are compared with emission spectra of PSII-cc obtained in this work from spinach and for dissolved PSII crystals from Thermosynechococcus (T.) elongatus. This new spectral datase...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-015-0128-7
更新日期：2015-06-01 00:00:00
abstract：:Flash-induced absorbance measurements at 830 nm on both nanosecond and microsecond timescales have been used to characterise the effect of ultraviolet light on Photosystem II core particles. A combination of UV-A and UV-B, closely simulating the spectrum of sunlight below 350 nm, was found to have a primary effect on ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00029424
更新日期：1996-07-01 00:00:00
abstract：:Quantum mechanics/molecular mechanics (QM/MM) hybrid methods are currently the most powerful computational tools for studies of structure/function relations and structural refinement of macrobiomolecules (e.g., proteins and nucleic acids). These methods are highly efficient, since they implement quantum chemistry tech...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-009-9467-6
更新日期：2009-11-01 00:00:00
abstract：:The first research paper on photosynthesis in China was published by T.T. Li(2) in 1929. Two photosynthesis laboratories were established in Shanghai and Beijing in the 1950s and the 1960s, respectively. A photophosphorylation 'intermediate' was discovered after the energy conversion process was separated into light a...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1024946222187
更新日期：2003-01-01 00:00:00
abstract：:The photosynthetic green sulfur bacterium Chlorobaculum (Cba.) tepidum produces bacteriochlorophyll (BChl) c pigments bearing a chiral 1-hydroxyethyl group at the 3-position, which self-aggregate to construct main light-harvesting antenna complexes, chlorosomes. The secondary alcoholic hydroxy group is requisite for c...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-016-0220-7
更新日期：2016-12-01 00:00:00
abstract：:Photosynthetic acclimation varies among species, which likely reveals variations at the biochemical level in the pathways that constitute carbon assimilation and energy transfer. Local adaptation and phenotypic plasticity affect the environmental response of photosynthesis. Phenotypic plasticity allows for a wide arra...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-013-9816-3
更新日期：2014-02-01 00:00:00
abstract：:Phosphatidylglycerol (PG), containing the unique fatty acid Delta3, trans-16:1-hexadecenoic acid, is a minor but ubiquitous lipid component of thylakoid membranes of chloroplasts and cyanobacteria. We investigated its role in electron transfers and structural organization of Photosystem II (PSII) by treating Arabidops...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-006-9126-0
更新日期：2007-07-01 00:00:00
abstract：:Isolated mesophyll cells from darkened leaves of the C(4) plant Digitaria sanguinalis keep functional plasmodesmata that allow the free exchange of low molecular mass compounds with the surrounding medium. This cell suspension system has been used to measure C(4) PEPC activity in situ using a spectrophotometric assay....
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/B:PRES.0000017179.31351.f0
更新日期：2004-01-01 00:00:00
abstract：:The discovery of a new maize (Zea mays L.) transposon system, Mutator, and the cloning of the 1.4 kilobase transposon, Mul, have made feasible the isolation of nuclear photosynthetic genes which are recognized only by their mutant phenotype. Mutant maize plants which express a high chlorophyll fluorescent (hcf) phenot...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00042979
更新日期：1988-10-01 00:00:00
abstract：:Cross-reconstitution of the extrinsic proteins and Photosystem II (PS II) from a green alga, Chlamydomonas reinhardtii, and a higher plant,Spinacia oleracea, was performed to clarify the differences of binding properties of the extrinsic proteins between these two species of organisms. (1) Chlamydomonas PsbP and PsbQ ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-004-7760-y
更新日期：2005-06-01 00:00:00
abstract：:We have used antibodies generated against synthetic peptides to determine the topology of the 43 kD chlorophyll a binding protein (CP 43) of Photosystem II. Based on the pattern of proteolytic fragments detected (on western blots) by peptide specific antibodies, a six transmembrane span topological model, with the ami...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00019041
更新日期：1994-04-01 00:00:00
abstract：:Four Indica and five Japonica varieties of rice (Oryza sativa L.) were examined to elucidate their differences in photosynthetic activity and dark respiratory rate as influenced by leaf nitrogen levels and temperatures. The photosynthetic rates of single leaf showed correlations with total nitrogen and soluble protein...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00032321
更新日期：1987-01-01 00:00:00
abstract：:Fluorescence and electron paramagnetic resonance measurements were used to study selenium influence on photosystem activity in rape seedlings affected by Cd stress. Water cultures containing Hoagland nutrients were supplemented with 400 microM of CdCl(2), 2 microM of Na(2)SeO(4) and a mixture of both CdCl(2) and Na(2)...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-010-9551-y
更新日期：2010-07-01 00:00:00
