Abstract:
:Electron transfer pathways in photosynthesis involve interactions between membrane-bound complexes such as reaction centres with an extrinsic partner. In this study, the biological specificity of electron transfer between the reaction centre-light-harvesting 1-PufX complex and its extrinsic electron donor, cytochrome c 2, formed the basis for mapping the location of surface-attached RC-LH1-PufX complexes using atomic force microscopy (AFM). This nano-mechanical mapping method used an AFM probe functionalised with cyt c 2 molecules to quantify the interaction forces involved, at the single-molecule level under native conditions. With surface-bound RC-His12-LH1-PufX complexes in the photo-oxidised state, the mean interaction force with cyt c 2 is approximately 480 pN with an interaction frequency of around 66 %. The latter value lowered 5.5-fold when chemically reduced RC-His12-LH1-PufX complexes are imaged in the dark to abolish electron transfer from cyt c 2 to the RC. The correspondence between topographic and adhesion images recorded over the same area of the sample shows that affinity-based AFM methods are a useful tool when topology alone is insufficient for spatially locating proteins at the surface of photosynthetic membranes.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Vasilev C,Brindley AA,Olsen JD,Saer RG,Beatty JT,Hunter CNdoi
10.1007/s11120-013-9812-7subject
Has Abstractpub_date
2014-05-01 00:00:00pages
169-80issue
1-2eissn
0166-8595issn
1573-5079journal_volume
120pub_type
杂志文章abstract::Isolated mesophyll cells from darkened leaves of the C(4) plant Digitaria sanguinalis keep functional plasmodesmata that allow the free exchange of low molecular mass compounds with the surrounding medium. This cell suspension system has been used to measure C(4) PEPC activity in situ using a spectrophotometric assay....
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/B:PRES.0000017179.31351.f0
更新日期:2004-01-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00034120
更新日期:1989-05-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00028793
更新日期:1992-04-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-013-9797-2
更新日期:2014-02-01 00:00:00
abstract::We evaluate low-temperature (low-T) emission spectra of photosystem II core complexes (PSII-cc) previously reported in the literature, which are compared with emission spectra of PSII-cc obtained in this work from spinach and for dissolved PSII crystals from Thermosynechococcus (T.) elongatus. This new spectral datase...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-015-0128-7
更新日期:2015-06-01 00:00:00
abstract::Chlorophyll fluorescence imaging has been used to analyse the response elicited in Phaseolus vulgaris after inoculation with Pseudomonas syringae pv. phaseolicola 1448A (compatible interaction) and P. syringae pv. tomato DC3000 (incompatible interaction). With the aim of modulating timing of symptom development, diffe...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-007-9278-6
更新日期:2008-04-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00032236
更新日期:1995-07-01 00:00:00
abstract::C4 photosynthesis is a complex trait that has a high degree of natural variation, involving anatomical and biochemical changes relative to the ancestral C3 state. It has evolved at least 66 times across a variety of lineages and the evolutionary route from C3 to C4 is likely conserved but not necessarily genetically i...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-013-9872-8
更新日期:2014-02-01 00:00:00
abstract::This concise review describes the current status of research into how carotenoids function in bacterial photosynthesis. This is illustrated by reference to very recent studies on both the photoprotective and antenna functions of carotenoids. The major remaining open questions on the detailed molecular mechanisms invol...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1014715114520
更新日期:2001-01-01 00:00:00
abstract::Induction of chlorophyll a fluorescence and photosynthesis as affected by temperature were measured in cucumber leaf discs. Abrupt changes of the maximal variable fluorescence, Fv(p), and photosynthesis were observed around 9° and 21°C when the temperature was decreased from 30° to 0°C. The temperature-dependent maxim...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00035259
更新日期:1988-02-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00020595
更新日期:1980-09-01 00:00:00
abstract::The biogenesis and oxygen-evolving activity of cyanobacterial Photosystem II (PSII) is dependent on a number of accessory proteins not found in the crystallised dimeric complex. These include Psb27, a small lipoprotein attached to the lumenal side of PSII, which has been assigned a role in regulating the assembly of t...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-011-9712-7
更新日期:2012-02-01 00:00:00
abstract::After a brief prologue on Otto Kandler's life, we describe briefly his pioneering work on photosynthesis (photophosphorylation and the carbon cycle) and his key participation in the discovery of the concept of three forms of life (Archaea, Prokarya, and Eukarya). With Otto Kandler's passing, both the international pho...
journal_title:Photosynthesis research
pub_type: 传,历史文章,杂志文章
doi:10.1007/s11120-018-0530-z
更新日期:2018-09-01 00:00:00
abstract::Phycobilisomes (PBS) are antenna complexes that harvest light for photosystem (PS) I and PS II in cyanobacteria and some algae. A process known as far-red light photoacclimation (FaRLiP) occurs when some cyanobacteria are grown in far-red light (FRL). They synthesize chlorophylls d and f and remodel PS I, PS II, and P...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-016-0303-5
更新日期:2017-02-01 00:00:00
abstract::Biogenesis of the photosynthetic apparatus in greening etiolated leaves of barley (Hordeum vulgare L) was investigated by an approach permitting investigation of this process under conditions that minimize differences in plastid development. Distributions of barley leaves greening for 24 h as to chlorophyll content an...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1016093127057
更新日期:2002-01-01 00:00:00
abstract::The previous work in our lab showed that the spinach chloroplast ATP synthase epsilon mutant with 3 amino acid residues deleted from the N-terminus had much lower ability to inhibit ATP hydrolysis and block proton leakage in comparison to a mutant with 1 or 2 residues deleted from the N-terminus. The present study aim...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-004-6554-6
更新日期:2005-01-01 00:00:00
abstract::Two-component regulatory systems that respond to changes in redox potential have recently been discovered in bacteria. 'Redox sensors' are defined as electron carriers which initiate control of gene expression upon oxidation or reduction. 'Redox response regulators' are defined as DNA-binding proteins which modify gen...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00016274
更新日期:1993-05-01 00:00:00
abstract::Effect of water-soluble and stable sucrose-bound iron oxyhydroxide nanoparticles [Fe[III] sucrose complex (FSC)] on the efficiency of electron transport in the photosystem II membranes was studied. FSC significantly increases (by a factor 1.5) the rate of light-induced oxygen evolution in the presence of alternative e...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-019-00647-4
更新日期:2019-10-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-009-9479-2
更新日期:2009-10-01 00:00:00
abstract::Triplet state electron paramagnetic resonance (EPR) experiments have been carried out at X-band on Rb. sphaeroides R-26 reaction centers that have been reconstituted with the carotenoid, spheroidene, and exchanged with 13(2)-OH-Zn-bacteriochlorophyll a and [3-vinyl]-13(2)-OH-bacteriochlorophyll a at the monomeric, 'ac...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00032823
更新日期:1993-09-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00017756
更新日期:1996-01-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00019595
更新日期:1987-01-01 00:00:00
abstract::Cassava, bean and maize leaves were fed with(14)CO2 in light and the primary products of photosynthesis identified 5 and 10 seconds after assimilation. In maize, approximately three quarters of the labelled carbon was incorporated in C4 acids, in beans about two thirds in PGA, and in cassava approximately 40-60% in C4...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00055123
更新日期:1987-01-01 00:00:00
abstract::Around 1960 experiments of Arnold and Clayton, Chance and Nishimura and Calvin and coworkers demonstrated that the primary photosynthetic electron transfer processes are not abolished by cooling to cryogenic temperatures. After a brief historical introduction, this review discusses some aspects of electron transfer in...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00041000
更新日期:1996-05-01 00:00:00
abstract::In plants, chlorophylls (chlorophyll a and chlorophyll b) are the most abundant tetrapyrrole molecules and are essential for photosynthesis. The first committed step of chlorophyll biosynthesis is the insertion of Mg(2+) into protoporphyrin IX, and thus subsequent steps of the biosynthesis are called the Mg branch. As...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-008-9291-4
更新日期:2008-05-01 00:00:00
abstract::Phosphatidylglycerol (PG), containing the unique fatty acid Delta3, trans-16:1-hexadecenoic acid, is a minor but ubiquitous lipid component of thylakoid membranes of chloroplasts and cyanobacteria. We investigated its role in electron transfers and structural organization of Photosystem II (PSII) by treating Arabidops...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-006-9126-0
更新日期:2007-07-01 00:00:00
abstract::The role of photosynthetic pigments in the development of separation methods in biochemistry during the period 1900-1980 is described beginning with M. Tswett who introduced separation of chlorophylls and carotenoids on columns and coined the term chromatography in 1906. In Uppsala, T. Svedberg developed the ultracent...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1024944606930
更新日期:2003-01-01 00:00:00
abstract::This review presents a summary of recent investigations examining the structure and function of the chlorophyll-proteins CPa-1 (CP47) and CPa-2 (CP43). Comparisons of the derived amino acid sequences of these proteins suggest sites for chlorophyll binding and for interactions between these chlorophyll-proteins and oth...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00032639
更新日期:1990-04-01 00:00:00
abstract::The chloroplast photo-oxidation and the expression of the Cab gene Lhcb1, encoding the Lhcb1 light-harvesting chlorophyll a/b-protein of PS II, have been studied in leaf cells of maize treated with the two bleaching herbicides norflurazon and amitrole and of the two carotenoid-free mutants vp9 and vp2 grown under high...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1006477215572
更新日期:2000-01-01 00:00:00
abstract::Detached wheat leaves were illuminated in air until a steady rate of photosynthesis was established. Then the gas was changed to 1% O2, 99% N2 and after 2.5 h further illumination the capacity of the leaves for photosynthesis in air was decreased to approximately 50%. Measurement of RuBP carboxylase activity in extrac...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00040708
更新日期:1982-06-01 00:00:00