Abstract:
:The biogenesis and oxygen-evolving activity of cyanobacterial Photosystem II (PSII) is dependent on a number of accessory proteins not found in the crystallised dimeric complex. These include Psb27, a small lipoprotein attached to the lumenal side of PSII, which has been assigned a role in regulating the assembly of the Mn(4)Ca cluster catalysing water oxidation. To gain a better understanding of Psb27, we have determined in this study the crystal structure of the soluble domain of Psb27 from Thermosynechococcus elongatus to a resolution of 1.6 Å. The structure is a four-helix bundle, similar to the recently published solution structures of Psb27 from Synechocystis PCC 6803 obtained by nuclear magnetic resonance (NMR) spectroscopy. Importantly, the crystal structure presented here helps us resolve the differences between the NMR-derived structural models. Potential binding sites for Psb27 within PSII are discussed in light of recent biochemical data in the literature.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Michoux F,Takasaka K,Boehm M,Komenda J,Nixon PJ,Murray JWdoi
10.1007/s11120-011-9712-7subject
Has Abstractpub_date
2012-02-01 00:00:00pages
169-75issue
3eissn
0166-8595issn
1573-5079journal_volume
110pub_type
杂志文章abstract::To determine the fluorescence properties of cyanobacterial Photosystem I (PS I) in relatively intact systems, fluorescence emission from 20 to 295 K and polarization at 77 K have been measured from phycobilisomes-less thylakoids of Synechocystis sp. PCC 6803 and a mutant strain lacking Photosystem II (PS II). At 295 K...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00028785
更新日期:1992-02-01 00:00:00
abstract::This article summarizes knowledge on genes and their respective proteins in the field of green algal hydrogen research. Emphasis is placed on recently cloned genes from the unicellular green alga Chlamydomonas reinhardtii, including HydA1 and HydA2, which encode homologous [Fe]-hydrogenases, Tla1, which encodes a chlo...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-004-2050-2
更新日期:2004-01-01 00:00:00
abstract::Bill Arnold's concept of photosynthesis as a solid state, as opposed to solution, phenomenon led him to an amazing series of fundamental discoveries. ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00040990
更新日期:1996-05-01 00:00:00
abstract::The first research paper on photosynthesis in China was published by T.T. Li(2) in 1929. Two photosynthesis laboratories were established in Shanghai and Beijing in the 1950s and the 1960s, respectively. A photophosphorylation 'intermediate' was discovered after the energy conversion process was separated into light a...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1024946222187
更新日期:2003-01-01 00:00:00
abstract::Photosynthetic acclimation varies among species, which likely reveals variations at the biochemical level in the pathways that constitute carbon assimilation and energy transfer. Local adaptation and phenotypic plasticity affect the environmental response of photosynthesis. Phenotypic plasticity allows for a wide arra...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-013-9816-3
更新日期:2014-02-01 00:00:00
abstract::The quantum yield for light-induced H2 generation was measured for a previously optimized bio-hybrid cytochrome c 6-crosslinked PSI(C13G)-1,8-octanedithiol-[FeFe]-H2ase(C97G) (PSI-H2ase) nanoconstruct. The theoretical quantum yield for the PSI-H2ase nanoconstruct is 0.50 molecules of H2 per photon absorbed, which equa...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-014-0064-y
更新日期:2016-01-01 00:00:00
abstract::The effect of desiccation and rehydration on the function of Photosystem II has been studied in the desiccation tolerant lichen Cladonia convoluta by thermoluminescence. We have shown that in functional fully hydrated thalli thermoluminescence signals can be observed from the recombination of the S2(3)QB (-) (B band),...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00041010
更新日期:1996-05-01 00:00:00
abstract::Characteristics of primary phases in chlorophyll a fluorescence transients based on room temperature in vivo measurement with a Plant Productivity Fluorometer (Brancker Model SF-10) can be greatly facilitated by coupling the instrument to a fast data acquisition system. The SF-10 was linked to a Multitech Industrial C...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00041817
更新日期:1983-01-01 00:00:00
abstract::Modified forms of the D1 protein with deletions in lumen-exposed regions, were constructed in the cyanobacterium Synechocystis 6803 using site-directed mutagenesis. Integration and stability of the mutated D1 proteins in the thylakoid membrane were studied by immunoblot and pulse-chase analyses. It was found that in Δ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00117663
更新日期:1996-08-01 00:00:00
abstract::A short list of the twentieth century's dominant figures in photosynthesis would unquestionably include Otto Warburg. One of his many discoveries, the 'bicarbonate effect' remains a lasting puzzle to his heirs in the field. Recent developments in this area of research have renewed interest and call for a re-examinatio...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1020447030191
更新日期:2002-01-01 00:00:00
abstract::When 23°C-grown potato leaves (Solanum tuberosum L.) were exposed for 15 min to elevated temperatures in weak light, a dramatic and preferential inactivation of Photosystem (PS) II was observed at temperatures higher than about 38°C. In vivo photoacoustic measurements indicated that, concomitantly with the loss of PS ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00017756
更新日期:1996-01-01 00:00:00
abstract::Phycobilisomes (PBS) are antenna complexes that harvest light for photosystem (PS) I and PS II in cyanobacteria and some algae. A process known as far-red light photoacclimation (FaRLiP) occurs when some cyanobacteria are grown in far-red light (FRL). They synthesize chlorophylls d and f and remodel PS I, PS II, and P...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-016-0303-5
更新日期:2017-02-01 00:00:00
abstract::A rapid procedure has been developed for the isolation of the photosystem two reaction centre complex (PS II RC) from a double mutant of Chlamydomonas reinhardtii, F54-14, which lacks the Photosystem one complex and the chloroplast ATPase. Thylakoid membranes are solubilised with 1.5% (w/v) Triton X-100 and the PS II ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00042974
更新日期:1995-02-01 00:00:00
abstract::The factors governing quinone protein binding in the photosynthetic bacterium Rps. viridis are investigated via use of empirical intermolecular energy calculations using the GRID method (PJ Goodford (1985) J Med Chem 28: 849-854). A probe atom corresponding to a carbonyl oxygen atom is used to investigate regions exhi...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00027142
更新日期:1994-01-01 00:00:00
abstract::Light activation of phosphoenolpyruvate carboxylase from the leaves of the C4 plant Setaria verticillata (L.) is more pronounced at low CO2 levels. The 2-fold activation observed at physiological ambient CO2 becomes 3.64-fold at 5 μL/L and completely abolished above 700 μL/L. When the stomata close under the influence...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00034834
更新日期:1988-11-01 00:00:00
abstract::An immunological method for quantitative determination of photosynthetic fructose-1,6-bisphosphatase in crude extracts of leaves is proposed. It is based on the ELISA technique, and offers two modifications. A non-competitive technique has a higher sensitivity and is the right option for samples of low fructose-1,6-bi...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00032710
更新日期:1987-01-01 00:00:00
abstract::With this Tribute, we remember and honor Thomas John (Tom) Wydrzynski. Tom was a highly innovative, independent and committed researcher, who had, early in his career, defined his life-long research goal. He was committed to understand how Photosystem II produces molecular oxygen from water, using the energy of sunlig...
journal_title:Photosynthesis research
pub_type: 传,历史文章,杂志文章
doi:10.1007/s11120-018-0606-9
更新日期:2019-06-01 00:00:00
abstract::Illumination of the dark-incubated cells of Synechocystis PCC6803 caused recovery of both respiratory activity of oxygen uptake and PS I-cyclic electron flow, which was monitored by the dark reduction of P700(+) in the presence of DCMU after a 50 ms pulse light (MT) under background far-red light, but the effects were...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1017946524199
更新日期:2001-01-01 00:00:00
abstract::The authors present a study of the fluorescence and absorbance transients occurring in whole cells of purple nonsulfur bacterium Rhodobacter sphaeroides on the millisecond timescale under pulsed actinic illumination. The fluorescence induction curve is interpreted in terms of combination of effects of redox changes in...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-010-9569-1
更新日期:2010-08-01 00:00:00
abstract::To understand design principles for assembling photosynthetic biohybrids that incorporate precisely-controlled sites for electron injection into redox enzyme cofactor arrays, we investigated the influence of chirality in assembly of the photosensitizer ruthenium(II)bis(2,2'-bipyridine)(4-bromomethyl-4'-methyl-2,2'-bip...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-019-00697-8
更新日期:2020-02-01 00:00:00
abstract::Productivity of most macroalgae is not currently considered limited by dissolved inorganic carbon (DIC), as the majority of species have CO2-concentrating mechanisms (CCM) allowing the active uptake of DIC. The alternative, diffusive uptake of CO2 (non-CCM), is considered rare (0-9% of all macroalgal cover in a given ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-015-0114-0
更新日期:2015-05-01 00:00:00
abstract::The 16th International Congress on Photosynthesis will be held August 11-16, 2013 in St. Louis, Missouri, USA. The congress will include 15 plenary lectures, 21 scientific symposia, poster sessions, exhibitors, opening reception, final banquet, excursions and accompanying persons program. The congress is organized as ...
journal_title:Photosynthesis research
pub_type:
doi:10.1007/s11120-013-9847-9
更新日期:2013-07-01 00:00:00
abstract::The effect of nitrate and nitrite on long-term chlorophyll fluorescence has been studied in filamentous blue-green algae. Cells grown autotrophically with nitrate as nitrogen source show, under argon atmosphere, a high level of fluorescence. The addition of either nitrete or nitrite induces a significant fluorescence ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00032356
更新日期:1981-09-01 00:00:00
abstract::Photosynthetic electron transfer has been examined in whole cells, isolated membranes and in partially purified reaction centers (RCs) of Roseicyclus mahoneyensis, strain ML6 and Porphyrobacter meromictius, strain ML31, two species of obligate aerobic anoxygenic phototrophic bacteria. Photochemical activity in strain ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-011-9718-1
更新日期:2012-02-01 00:00:00
abstract::Oxygenic photosynthesis is driven by two multi-subunit membrane protein complexes, Photosystem I and Photosystem II. In plants and green algae, both complexes are composed of two moieties: a reaction center (RC), where light-induced charge translocation occurs, and a peripheral antenna that absorbs light and funnels i...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/B:PRES.0000036881.23512.42
更新日期:2004-01-01 00:00:00
abstract::Soybean [Glycine max (L.) Merr. cv. Williams 82 and A3127] plants were grown in the field under long-term soil moisture deficit and irrigation to determine the effects of severe drought stress on the photosynthetic capacity of soybean leaves. Afternoon leaf water potentials, stomatal conductances, intercellular CO2 co...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00051735
更新日期:1990-07-01 00:00:00
abstract::Recent magnetic-resonance work on YŻ suggests that this species exhibits considerable motional flexibility in its functional site and that its phenol oxygen is not involved in a well-ordered hydrogen-bond interaction (Tang et al., submitted; Tommos et al., in press). Both of these observations are inconsistent with a ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00020428
更新日期:1995-11-01 00:00:00
abstract::The oxygen production of dark-adapted Photosystem II upon illumination by a series of single-turnover flashes shows a damped period four oscillation with flash number. The damping is attributed to 'misses' resulting from a statistical probability that a reaction center fails to produce a stable charge separation after...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1016128632704
更新日期:2002-01-01 00:00:00
abstract::Oxygenic photosynthesis requires chlorophyll (Chl) for the absorption of light energy, and charge separation in the reaction center of photosystem I and II, to feed electrons into the photosynthetic electron transfer chain. Chl is bound to different Chl-binding proteins assembled in the core complexes of the two photo...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-015-0154-5
更新日期:2015-12-01 00:00:00
abstract::Biogenesis of the photosynthetic apparatus in greening etiolated leaves of barley (Hordeum vulgare L) was investigated by an approach permitting investigation of this process under conditions that minimize differences in plastid development. Distributions of barley leaves greening for 24 h as to chlorophyll content an...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1016093127057
更新日期:2002-01-01 00:00:00