Abstract：
:The thylakoid membrane protein PsbS is critical for quenching excessive excitation energy in mechanisms that involve the light-harvesting complexes of photosystem II. Liposomes of thylakoid lipids have been shown to be a very good platform to study photosynthetic membrane proteins and their interactions. In this study, we simultaneously refolded and reconstituted functional pea PsbS into liposomes of thylakoid lipids starting from denatured expressed protein. Intrinsic fluorescence spectroscopy, trypsin digestion, and circular dichroism spectroscopy were used to characterize the native state of PsbS in the proteoliposomes. The functionality of refolded PsbS was further demonstrated by its effect on the fluorescence quenching of the major antenna system of photosystem II (LHCII) co-inserted into the liposomes. The fluorescence yield of native trimeric LHCII was lowered by PsbS by 50% at neutral pH and by a further 25% upon lowering the pH to 4.5. Furthermore, the acid-induced fluorescence reduction was completely reversed by addition of N,N'-dicyclohexylcarbodiimide, an inhibitor of protein protonation. These results indicate that reconstituted PsbS induces strong quenching of LHCII sensing changes in local pH via its protonation sites.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Liu C,Gao Z,Liu K,Sun R,Cui C,Holzwarth AR,Yang Cdoi
10.1007/s11120-015-0176-zsubject
Has Abstractpub_date
2016-01-01 00:00:00pages
109-16issue
1eissn
0166-8595issn
1573-5079pii
10.1007/s11120-015-0176-zjournal_volume
127pub_type
杂志文章abstract：:The functional peculiarities and responses of the photosynthetic system in the flowering homoiochlorophyllous desiccation-tolerant (HDT) Haberlea rhodopensis and the non-desiccation-tolerant spinach were compared during desiccation and rehydration. Increasing rate of water loss clearly modifies the kinetic parameters ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-005-2440-0
更新日期：2005-08-01 00:00:00
abstract：:Recently, microalgae have attracted attention as sources of biomass energy. However, fatty acids from the microalgae are mainly unsaturated and show low stability in oxygenated environments, due to oxidation of the double bonds. The branched-chain fatty acid, 10-methyl stearic acid, is synthesized from oleic acid in c...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-018-0537-5
更新日期：2019-03-01 00:00:00
abstract：:The nature of interaction of cytochrome b-559 high potential (HP) with electron transport on the reducing side of photosystem II was investigated by measuring the susceptibility of cytochrome b-559HP to 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) under different conditions. Submicromolar DCMU concentrations decreas...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00035839
更新日期：1991-03-01 00:00:00
abstract：:The coupling factor CF1 is a catalytic part of chloroplast ATP synthase which is exposed to stroma whose viscosity is many-fold higher than that of reaction mixtures commonly used to measure kinetics of CF1-catalyzed ATP hydrolysis. This study is focused on the effect of medium viscosity modulated by sucrose or bovine...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-015-0213-y
更新日期：2016-05-01 00:00:00
abstract：:Analysis of leaf-level photosynthetic responses of 39 tree species grown in elevated concentrations of atmospheric CO2 indicated an average photosynthetic enhancement of 44% when measured at the growth [CO2]. When photosynthesis was measured at a common ambient [CO2], photosynthesis of plants grown at elevated [CO2] w...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00014592
更新日期：1994-03-01 00:00:00
abstract：:Pigment-protein-complexes of two chlorophyll b deficient mutants of Arabidopsis and from the wild type were separated electrophoretically. Light-harvesting proteins were absent in the chlorophyll b free mutant ch(1) and their amount was reduced in the mutant ch(2) which has a reduced content of chlorophyll b. The rati...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00041827
更新日期：1983-01-01 00:00:00
abstract：:The storage of light energy in chemical form through photosynthesis is the key process underlying life as we know it. To utilize photosynthates efficiently as structural materials or as fuel to drive endergonic processes, they have to be transported from where they are produced to where they are needed. In this primer...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-013-9867-5
更新日期：2013-11-01 00:00:00
abstract：:The author gives an autobiographical sketch of his path to chlorophyll research, and describes some results. The discussion is largely focused on long wavelength forms of chlorophyll and how they might be generated by self-assembly. Dimers or oligomers, (Chl)n, result from coordination interactions between the central...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00033128
更新日期：1990-12-01 00:00:00
abstract：:Unique light-harvesting antennas in the green sulfur bacterium Chlorobaculum tepidum, called chlorosomes, consist of self-aggregates of bacteriochlorophyll (BChl) c. In the biosynthesis of BChl c, BciC demethoxycarbonylase removes the C132-methoxycarbonyl group to facilitate the self-aggregation of BChl c. We previous...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-018-0573-1
更新日期：2019-03-01 00:00:00
abstract：:Here, we describe a new imaging setup able to assess in vivo photosynthetic activity. The system specifically measures time-resolved chlorophyll fluorescence in response to light. It is composed of a fast digital camera equipped with a wide-angle lens for the analysis of samples up to 10 x 10 cm, i.e. entire plants or...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-009-9487-2
更新日期：2009-10-01 00:00:00
abstract：:Most chloroplasts undergo changes in composition, function and structure in response to growth irradiance. However, Tradescantia albiflora, a facultative shade plant, is unable to modulate its light-harvesting components and has the same Chl a/Chl b ratios and number of functional PS II and PS I reaction centres on a ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1010681721929
更新日期：2001-01-01 00:00:00
abstract：:A comparative study of photosystem II complexes isolated from tobacco (Nicotiana tabacum L. cv. John William's Broadleaf) which contains normal stacked thylakoid membranes, and from two chlorophyll deficient tobacco mutants (Su/su and Su/su var. Aurea) which have low stacked grana or essentially unstacked thylakoids w...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00032264
更新日期：1987-01-01 00:00:00
abstract：:In order to evaluate genotypic variation, we measured leaf photosynthetic rate (Pn) and its associated factors for the rice diversity research set of germplasm (RDRS) selected from the Genebank in National Institute of Agrobiological Sciences (NIAS). Pn showed large genotypic variation from 11.9 to 32.1 micromol m(-2 ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-007-9208-7
更新日期：2007-10-01 00:00:00
abstract：:The photoenzymatic cycle of the light-dependent NADPH:protochlorophyllide oxidoreductase (LPOR) was investigated in situ during early stages of development of bean leaves under light-dark cycles (LDC). In the experimental system used in this study, prolamellar bodies developed during night periods and disappeared duri...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-007-9274-x
更新日期：2008-04-01 00:00:00
abstract：:Pulse modulated fluorescence has increasingly been used as an ecological tool to examine changes in the vertical distribution of microphytobenthic cells within the upper layers of estuarine sediments (most often using the minimum fluorescence yield F(o)) as well as to indicate the health of the community (using the ma...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/B:PRES.0000028397.86495.b5
更新日期：2004-01-01 00:00:00
abstract：:Cross-reconstitution of the extrinsic proteins and Photosystem II (PS II) from a green alga, Chlamydomonas reinhardtii, and a higher plant,Spinacia oleracea, was performed to clarify the differences of binding properties of the extrinsic proteins between these two species of organisms. (1) Chlamydomonas PsbP and PsbQ ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-004-7760-y
更新日期：2005-06-01 00:00:00
abstract：:This work addresses the question of occurrence and function of photosystem II (PSII) in bundle sheath (BS) cells of leaves possessing NADP-malic enzyme-type C4 photosynthesis (Zea mays). Although no requirement for PSII activity in the BS has been established, several component proteins of PSII have been detected in B...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-014-0009-5
更新日期：2014-10-01 00:00:00
abstract：:The world is facing energy crisis and environmental issues due to the depletion of fossil fuels and increasing CO2 concentration in the atmosphere. Growing microalgae can contribute to practical solutions for these global problems because they can harvest solar energy and capture CO2 by converting it into biofuel usin...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-013-9828-z
更新日期：2013-11-01 00:00:00
abstract：:The D1/D2 heterodimer core is the heart of the photosystem II reaction center. A characteristic feature of this heterodimer is the differentially rapid, light-dependent degradation of the D1 protein. The D1 protein is possibly the most researched photosynthetic polypeptide, with aspects of structure-function, gene, me...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-008-9342-x
更新日期：2008-10-01 00:00:00
abstract：:In this communication, we report our progress on the development of low-frequency Fourier transform infrared (FTIR) spectroscopic techniques to study metal-substrate and metal-ligand vibrational modes in the Photosystem II/oxygen-evolving complex (PS II/OEC). This information will provide important structural and mech...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1010779327960
更新日期：2000-01-01 00:00:00
abstract：:Solubilisation of thylakoid membranes from young leaves of Pisum sativum in the presence of Triton X-100 resulted in an almost complete loss of quenching of light-harvesting chlorophyll-protein (LHCP) fluorescence, as measured at 77°K. There were concomitant changes in the kinetics of light-saturation curves of electr...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00037130
更新日期：1986-01-01 00:00:00
abstract：:Four Indica and five Japonica varieties of rice (Oryza sativa L.) were examined to elucidate their differences in photosynthetic activity and dark respiratory rate as influenced by leaf nitrogen levels and temperatures. The photosynthetic rates of single leaf showed correlations with total nitrogen and soluble protein...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00032321
更新日期：1987-01-01 00:00:00
abstract：:This review describes the progress in our understanding of the structure of the Mn complex in Photosystem II over the last two decades. Emphasis is on the research from our laboratory, especially the results from X-ray absorption spectroscopy, low temperature electron paramagnetic resonance and electron spin echo enve...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00046751
更新日期：1993-01-01 00:00:00
abstract：:In vitro studies of the carotenoid peridinin, which is the primary pigment from the peridinin chlorophyll-a protein (PCP) light harvesting complex, showed a strong dependence on the lifetime of the peridinin lowest singlet excited state on solvent polarity. This dependence was attributed to the presence of an intramol...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-005-1447-x
更新日期：2005-11-01 00:00:00
abstract：:A correlated influence of cation concentration and excitation energy level on PS II Abbreviations: AMPD = 2-amino-2-methyl-1,3-propanediol; DCIP = 2-6 dichlorophenol indophenol; DCIPH2 = DCIP in reduced state; DCIPr = rate of DCIP-reduction; DCMU = 3-(3,4 dichlorophenyl)1-1 dimethyl-urea; Fv = variable fluorescence; F...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00018228
更新日期：1980-06-01 00:00:00
abstract：:Cyanobacteria exhibit a novel form of non-photochemical quenching (NPQ) at the level of the phycobilisome. NPQ is a process that protects photosystem II (PSII) from possible highlight-induced photo-damage. Although significant advancement has been made in understanding the NPQ, there are still some missing details. Th...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-018-0498-8
更新日期：2018-08-01 00:00:00
abstract：:Brownian Dynamics (BD) computer simulations were used to study electrostatic interactions between turnip cytochrome f (cyt f) and spinach plastocyanin (PC). Three different spinach PC structures were studied: The X-ray crystal structure of Xue and coworkers [(1998) Protein Sci 7:2099-2105] and the NMR structure of Mus...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-007-9192-y
更新日期：2007-11-01 00:00:00
abstract：:Light-harvesting Chl a/b protein complexes were isolated from the higher plant Sinapis alba, the green alga Chlorella fusca, and the prasinophycean alga Mantoniella squamata by mild gel electrophoresis. The energy transfer from chlorophyll b and the accessory xanthophyll was measured by means of fluoresence spectrosco...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00035234
更新日期：1987-01-01 00:00:00
abstract：:A guided tour through much of photosynthesis research as I saw it, 1936-2001, is presented here. For earlier perspectives, see Myers 1974 (Plant Physiol 54: 420-426) and 1996 (Photosynth Res 50: 195-208). ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1020475502933
更新日期：2002-01-01 00:00:00
abstract：:Oxygen evolving photosystem II particles were exposed to 100 and 250 W m(-2) white light at 20°C under aerobic, anaerobic and strongly reducing (presence of dithionite) conditions. Three types of photoinactivation processes with different kinetics could be distinguished: (1) The fast process which occurs under strongl...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00030061
更新日期：1990-01-01 00:00:00
