Abstract:
:This work addresses the question of occurrence and function of photosystem II (PSII) in bundle sheath (BS) cells of leaves possessing NADP-malic enzyme-type C4 photosynthesis (Zea mays). Although no requirement for PSII activity in the BS has been established, several component proteins of PSII have been detected in BS cells of developing maize leaves exhibiting O2-insensitive photosynthesis. We used the basal fluorescence emissions of PSI (F 0I) and PSII (F 0II) as quantitative indicators of the respective relative photosystem densities. Chl fluorescence induction was measured simultaneously at 680 and 750 nm. In mature leaves, the F m(680)/F 0(680) ratio was 10.5 but less in immature leaves. We propose that the lower ratio was caused by the presence of a distinct non-variable component, F c, emitting at 680 and 750 nm. After F c was subtracted, the fluorescence of PSI (F 0I) was detected as a non-variable component at 750 nm and was undetectably low at 680 nm. Contents of Chls a and b were measured in addition to Chl fluorescence. The Chl b/(a + b) was relatively stable in developing sunflower leaves (0.25-0.26), but in maize it increased from 0.09 to 0.21 with leaf tissue age. In sunflower, the F 0I/(F 0I + F 0II) was 0.39 ± 0.01 independent of leaf age, but in maize, this parameter was 0.65 in young tissue of very low Chl content (20-50 mg m(-2)) falling to a stable level of 0.53 ± 0.01 at Chl contents >100 mg m(-2). The values of F 0I/(F 0I + F 0II) showed that in sunflower, excitation was partitioned between PSII and PSI in a ratio of 2:1, but the same ratio was 1:1 in the C4 plant. The latter is consistent with a PSII:PSI ratio of 2:1 in maize mesophyll cells and PSI only in BS cells (2:1:1 distribution). We suggest, moreover, that redox mediation of Chl synthesis, rather than protein accumulation, regulates photosystem assembly to ensure optimum excitation balance between functional PSII and PSI. Indeed, the apparent necessity for two Chls (a and b) may reside in their targeted functions in influencing accumulation of PSI and PSII, respectively, as opposed to their spectral differences.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Peterson RB,Oja V,Eichelmann H,Bichele I,Dall'Osto L,Laisk Adoi
10.1007/s11120-014-0009-5subject
Has Abstractpub_date
2014-10-01 00:00:00pages
41-56issue
1eissn
0166-8595issn
1573-5079journal_volume
122pub_type
杂志文章abstract::Cyanobacteria perform photosynthesis with the use of large light-harvesting antennae called phycobilisomes (PBSs). These hemispherical PBSs contain hundreds of open-chain tetrapyrrole chromophores bound to different peptides, providing an arrangement in which excitation energy is funnelled towards the PBS core from wh...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-017-0417-4
更新日期:2018-03-01 00:00:00
abstract::Time-resolved (TR) infrared (IR) spectroscopy in the nanosecond to second timescale has been extensively used, in the last 30 years, in the study of photosynthetic systems. Interesting results have also been obtained at lower time resolution (minutes or even hours). In this review, we first describe the used technique...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-016-0305-3
更新日期:2017-02-01 00:00:00
abstract::The fluorescence kinetics of both intact needles and isolated chloroplasts of summer active and winter stressed Pinus sylvestris were measured at both room temperature and 77 K. It was confirmed that winter stress inhibited the photochemical capacity of photosystem II but also that winter stress caused the strongest i...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00032919
更新日期:1985-01-01 00:00:00
abstract::Two green algal species, Chlamydomonas reinhardtii and Scenedesmus obliquus, exhibited a relative maximum during the decay of luminescence, when adapted to low CO2 conditions that was not observed in high CO2 adapted cells.From the kinetics of transient changes in the level of dark fluorescence, after illumination and...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00034857
更新日期:1990-03-01 00:00:00
abstract::Phosphoenolpyruvate carboxylase from leaves of the C4 plant Setaria verticillata (L.) Beauv. is activated by light; day levels of activity are reached after 30 minutes of illumination. Photoactivation is prevented by inhibitors of photosynthetic electron flow or of photophosphorylation and by D,L-glyceraldehyde, which...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00028842
更新日期:1988-06-01 00:00:00
abstract::A synthetic fluorochromous lipid, rhodaminyl triglyceride (rhodaminyl TG), was intercalated into isolated thylakoid membranes of chloroplasts up to 30 molecules per 100 molecules of chlorophyll. As a result of fluorochrome presence, an absorption band appeared in a yellow-green spectrum region, its intensity being com...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00047079
更新日期:1990-11-01 00:00:00
abstract::A new binding site for anions which inhibit the water oxidizing complex (WOC) of Photosystem II in spinach has been identified. Anions which bind to this site inhibit the flash-induced S2/S0 catalase reaction (2H2O2→2H2O+O2) of the WOC by displacing hydrogen peroxide. Using a mass spectrometer and gas permeable membra...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00046771
更新日期:1993-01-01 00:00:00
abstract::Three Chl-protein complexes were isolated from thylakoid membranes of Bryopsis maxima and Ulva pertusa, marine green algae that inhabit the intertidal zone of the Pacific Ocean off the eastern coast of Japan by dodecyl-beta-D-maltoside polyacrylamide gel electrophoresis. The slowest-moving fractions showed low Chl a/b...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-006-9064-x
更新日期:2006-07-01 00:00:00
abstract::In this brief report, we provide a pictorial essay on an international conference "Photosynthesis Research for Sustainability-2013 in honor of Jalal A. Aliyev" that was held in Baku, Azerbaijan, during June 5-9, 2013 ( http://photosynthesis2013.cellreg.org/ ). We begin this report with a brief note on Jalal Aliyev, th...
journal_title:Photosynthesis research
pub_type:
doi:10.1007/s11120-013-9901-7
更新日期:2013-12-01 00:00:00
abstract::Pulse modulated fluorescence has increasingly been used as an ecological tool to examine changes in the vertical distribution of microphytobenthic cells within the upper layers of estuarine sediments (most often using the minimum fluorescence yield F(o)) as well as to indicate the health of the community (using the ma...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/B:PRES.0000028397.86495.b5
更新日期:2004-01-01 00:00:00
abstract::P700 enriched fractions were isolated from two brown algae and one diatom using sucrose density centrifugation after digitinin solubilization. They had a Chl a/P700 ratio of about 250 to 375 according to the species, they were enriched in long-wavelength absorbing Chl a and exhibited a fluorescence emission maximum at...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00035009
更新日期:1990-02-01 00:00:00
abstract::Precise control of the proton concentration gradient across thylakoid membranes (ΔpH) is essential for photosynthesis and its regulation because the gradient contributes to the generation of the proton motive force used for ATP synthesis and also for the fast and reversible induction of non-photochemical quenching (NP...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-018-0575-z
更新日期:2019-03-01 00:00:00
abstract::The seasonal maximum in photosynthetic CO2 exchange rate (CER) and the cessation of leaf expansion in soybeans (Glycine max (L.) Merr) accompany fruiting under normal agricultural conditions. To investigate whether these phenomena were obligatively tied together, we caused early flowering of long-season varieties by i...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00034108
更新日期:1982-12-01 00:00:00
abstract::A three-dimensional model of the core proteins D1 and D2, including the cofactors, that form the Photosystem II reaction centre of pea (Pisum sativum), has been generated. This model was built with a rule-based computer modelling system using the information from the crystal structures of the photosynthetic reaction c...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00033446
更新日期:1992-11-01 00:00:00
abstract::This contribution gives a brief survey of the short history since the discovery of the aerobic phototrophic bacteria to focus on a general evolutionary scenario. Most of the citations are of reviews that have covered the earlier literature and to which the reader is directed at appropriate places in the following text...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1020493518379
更新日期:2002-01-01 00:00:00
abstract::The binding characteristics and the inhibitory power of atrazine and DCMU towards uncoupled electron flow activity were studied in acyl lipid-depleted thylakoid membranes from atrazine-susceptible and-resistant biotypes of Solanum nigrum L. For this purpose, phospholipase A2 from Vipera russelli and the lipase from Rh...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00049537
更新日期:1992-01-01 00:00:00
abstract::Photosystem I particles containing 30-40 chlorophyll a molecules per primary electron donor P700 were subjected to 1.5 ps low density laser flashes at 610 nm resulting in excitation of the antenna chlorophyll a molecules followed by energy transfer to P700 and subsequent oxidation of P700. Absorbance changes were moni...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00047947
更新日期:1987-01-01 00:00:00
abstract::The coupling factor CF1 is a catalytic part of chloroplast ATP synthase which is exposed to stroma whose viscosity is many-fold higher than that of reaction mixtures commonly used to measure kinetics of CF1-catalyzed ATP hydrolysis. This study is focused on the effect of medium viscosity modulated by sucrose or bovine...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-015-0213-y
更新日期:2016-05-01 00:00:00
abstract::Moderate heat stress and fluctuating light are typical conditions in summer in tropical and subtropical regions. This type of stress can cause photodamage to photosystems I and II (PSI and PSII). However, photosynthetic responses to the combination of heat and fluctuating light in young leaves are little known. In thi...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-020-00754-7
更新日期:2020-06-01 00:00:00
abstract::The xanthophyll cycle represents one of the important photoprotection mechanisms in plant cells. In the present review, we summarize current knowledge about the violaxanthin cycle of vascular plants, green and brown algae, and the diadinoxanthin cycle of the algal classes Bacillariophyceae, Xanthophyceae, Haptophyceae...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-010-9536-x
更新日期:2010-11-01 00:00:00
abstract::Peridinin-chlorophyll-protein (PCP), containing differently absorbing chlorophyll derivatives, are good models with which to study energy transfer among monomeric chlorophylls (Chls) by both bulk and single-molecule spectroscopy. They can be obtained by reconstituting the N-terminal domain of the protein (N-PCP) with ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-007-9277-7
更新日期:2008-02-01 00:00:00
abstract::Polyamines (PAs) are small metabolites that are produced and oxidized in chloroplasts with an obscure mode of action. Recently, we showed that qE is stimulated by PAs in higher plants (Nicotiana tabacum) and in genetically modified plants with elevated thylakoid-associated PAs (Ioannidis and Kotzabasis Biochim Biophys...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-010-9617-x
更新日期:2011-02-01 00:00:00
abstract::By recording leaf transmittance at 820 nm and quantifying the photon flux density of far red light (FRL) absorbed by long-wavelength chlorophylls of Photosystem I (PS I), the oxidation kinetics of electron carriers on the PS I donor side was mathematically analyzed in sunflower (Helianthus annuus L.), tobacco (Nicotia...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1026070612022
更新日期:2003-01-01 00:00:00
abstract::The thylakoid membrane protein PsbS is critical for quenching excessive excitation energy in mechanisms that involve the light-harvesting complexes of photosystem II. Liposomes of thylakoid lipids have been shown to be a very good platform to study photosynthetic membrane proteins and their interactions. In this study...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-015-0176-z
更新日期:2016-01-01 00:00:00
abstract::In the leaf succulent Asclepiad Hoya carnosa (L.) R. Br., CAM photosynthesis occurred under well-watered conditions, as characterized by diurnal gas exchange and changes in titratable acidity. Following 10-12 days of severe water stress, the plants shifted from CAM to a modified CAM-idling mode of metabolism. CAM-idli...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00052124
更新日期:1983-09-01 00:00:00
abstract::Relationships between induced high leaf intercellular CO₂ concentrations, leaf K⁺ and NO₃⁻ ion movement and early fruit formation under macronutrient limitation are not well understood. We examined the effects and interactions of reduced K/N input treatments on leaf intercellular CO₂, photosynthesis rate, carboxylatio...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-013-9832-3
更新日期:2013-07-01 00:00:00
abstract::Chloroplast NADP-dependent malate dehydrogenase (NADP-MDH, EC 1.1.1.82) is inactive in the dark and activated in the light via a reduction of specific disulfides by thiol-disulfide interchange with thioredoxin, reduced by the photosynthetic electron transfer. Compared to the constitutively active NAD-dependent forms, ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1016099228450
更新日期:2002-01-01 00:00:00
abstract::The discovery of a new maize (Zea mays L.) transposon system, Mutator, and the cloning of the 1.4 kilobase transposon, Mul, have made feasible the isolation of nuclear photosynthetic genes which are recognized only by their mutant phenotype. Mutant maize plants which express a high chlorophyll fluorescent (hcf) phenot...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00042979
更新日期:1988-10-01 00:00:00
abstract::The origin of the extraordinary high redox potential of P680, the primary electron donor of Photosystem II, is still unknown. Photochemically induced dynamic nuclear polarisation (photo-CIDNP) 13C magic-angle spinning (MAS) NMR is a powerful method to study primary electron donors. In order to reveal the electronic st...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-005-0411-0
更新日期:2005-06-01 00:00:00
abstract::A non-competitive enzyme-linked immunosorbent assay (ELISA) which enables detection of as little as 0.1 ng cytochrome f in leaf extracts has been developed. No evidence for specific or non-specific interference by proteins other than cytochrome f was found. The assay was applied to a comparative study of age-related c...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00032649
更新日期:1990-04-01 00:00:00