Abstract:
:Photosystem I particles containing 30-40 chlorophyll a molecules per primary electron donor P700 were subjected to 1.5 ps low density laser flashes at 610 nm resulting in excitation of the antenna chlorophyll a molecules followed by energy transfer to P700 and subsequent oxidation of P700. Absorbance changes were monitored as a function of time with 1.5 ps time resolution. P700 bleaching (decrease in absorbance) occurred within the time resolution of the experiment. This is attributed to the formation of (1)P700.(*) This observation was confirmed by monitoring the rise of a broad absorption band near 810 nm due to chlorophyll a excited singlet state formation. The appearance of the initial bleach at 700 nm was followed by a strong bleaching at 690 nm. The time constant for the appearance of the 690 nm bleach is 13.7±0.8 ps. In the near-infrared region of the spectrum, the 810 nm band (which formed upon the excitation of the photosystem I particles) diminished to about 60% of its original intensity with the same 13.7 ps time constant as the formation of the 690 nm band. The spectral changes are interpreted as due to the formation of the charge separated state P700(+)-A0 (-), where A0 is the primary electron acceptor chlorophyll a molecule.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Wasielewski MR,Fenton JM,Govindjeedoi
10.1007/BF00047947subject
Has Abstractpub_date
1987-01-01 00:00:00pages
181-9issue
2eissn
0166-8595issn
1573-5079journal_volume
12pub_type
杂志文章abstract::The chlorophyll content is an important experimental parameter in agronomy and plant biology research. In this report, we explore the feasibility of determining total concentration of extracts containing chlorophyll a and chlorophyll b by chlorophyll fluorescence. We found that an excitation at 457 nm results in the s...
journal_title:Photosynthesis research
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章,评审
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journal_title:Photosynthesis research
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更新日期:1987-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/BF00042974
更新日期:1995-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2007-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:1984-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2001-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2008-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/BF00019595
更新日期:1987-01-01 00:00:00
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pub_type: 传,历史文章,杂志文章
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pub_type: 杂志文章
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更新日期:2017-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1023/A:1016093127057
更新日期:2002-01-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2000-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2007-06-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1995-03-01 00:00:00
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更新日期:1988-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:1991-05-01 00:00:00
abstract::The Photosystem I reaction centre protein CP1, isolated from barley using polyacrylamide gel electrophoresis showed an EPR (Electron Paramgnetic Resonance) spectrum with the polarisation pattern AEEAAE, typical of the primary donor triplet state (3)P700, created via radical pair formation and recombination. (3)P700 co...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00034795
更新日期:1992-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:2014-10-01 00:00:00