Abstract:
:A rapid procedure has been developed for the isolation of the photosystem two reaction centre complex (PS II RC) from a double mutant of Chlamydomonas reinhardtii, F54-14, which lacks the Photosystem one complex and the chloroplast ATPase. Thylakoid membranes are solubilised with 1.5% (w/v) Triton X-100 and the PS II RC purified by anion-exchange chromatography using TSK DEAE-650(S) (Merck). The complex has a pigment stoichiometry of approximately six chlorophyll a: two pheophytin a: one cytochrome b-559: one to two β-carotene. It photoaccumulates reduced pheophytin and oxidised P680 in the presence of sodium dithionite and silicomolybdate, respectively. Immunoblotting experiments have confirmed the presence of the D1 and D2 polypeptides in this complex. The α-subunit of cytochrome b-559 was identified by N-terminal sequencing. Comparison of the complex with the PS II RC from pea using SDS-polyacrylamide gel electrophoresis showed that their polypeptide compositions were similar. However, the α-subunit of cytochrome b-559 from C. reinhardtii has a lower apparent molecular weight than the pea counterpart whereas the β-subunit is larger.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Alizadeh S,Nixon PJ,Telfer A,Barber Jdoi
10.1007/BF00042974subject
Has Abstractpub_date
1995-02-01 00:00:00pages
165-71issue
2eissn
0166-8595issn
1573-5079journal_volume
43pub_type
杂志文章abstract::The study of the light-harvesting complex II (LHC-II) phosphatase activity has been difficult due to the membrane association of its substrate. Thylakoid membranes labeled with [γ-(32)P]ATP were incubated with chymotrypsin, releasing phosphopeptides which served as labeled substrates for LHC-II phosphatase. Utilizing ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00018301
更新日期:1995-05-01 00:00:00
abstract::In order to evaluate genotypic variation, we measured leaf photosynthetic rate (Pn) and its associated factors for the rice diversity research set of germplasm (RDRS) selected from the Genebank in National Institute of Agrobiological Sciences (NIAS). Pn showed large genotypic variation from 11.9 to 32.1 micromol m(-2 ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-007-9208-7
更新日期:2007-10-01 00:00:00
abstract::The coupling factor CF1 is a catalytic part of chloroplast ATP synthase which is exposed to stroma whose viscosity is many-fold higher than that of reaction mixtures commonly used to measure kinetics of CF1-catalyzed ATP hydrolysis. This study is focused on the effect of medium viscosity modulated by sucrose or bovine...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-015-0213-y
更新日期:2016-05-01 00:00:00
abstract::Chlorosomes of green photosynthetic bacteria are the most amazing example of long-range ordered natural light-harvesting antennae. Chlorosomes are the largest among all known photosynthetic light-harvesting structures (~ 104-105 pigments in the aggregated state). The chlorosomal bacteriochlorophyll (BChl) c/d/e molecu...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-019-00707-9
更新日期:2020-12-01 00:00:00
abstract::We have used antibodies generated against synthetic peptides to determine the topology of the 43 kD chlorophyll a binding protein (CP 43) of Photosystem II. Based on the pattern of proteolytic fragments detected (on western blots) by peptide specific antibodies, a six transmembrane span topological model, with the ami...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00019041
更新日期:1994-04-01 00:00:00
abstract::By recording leaf transmittance at 820 nm and quantifying the photon flux density of far red light (FRL) absorbed by long-wavelength chlorophylls of Photosystem I (PS I), the oxidation kinetics of electron carriers on the PS I donor side was mathematically analyzed in sunflower (Helianthus annuus L.), tobacco (Nicotia...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1026070612022
更新日期:2003-01-01 00:00:00
abstract::The binding characteristics and the inhibitory power of atrazine and DCMU towards uncoupled electron flow activity were studied in acyl lipid-depleted thylakoid membranes from atrazine-susceptible and-resistant biotypes of Solanum nigrum L. For this purpose, phospholipase A2 from Vipera russelli and the lipase from Rh...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00049537
更新日期:1992-01-01 00:00:00
abstract::The picture presently emerging from studies on the mechanism of photosystem II electron transport is discussed. The reactions involved in excitation trapping, charge separation and stabilization of the charge pair in the reaction center, followed by the reactions with the substrates, plastoquinone reduction and water ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00032785
更新日期:1985-01-01 00:00:00
abstract::Time-resolved (TR) infrared (IR) spectroscopy in the nanosecond to second timescale has been extensively used, in the last 30 years, in the study of photosynthetic systems. Interesting results have also been obtained at lower time resolution (minutes or even hours). In this review, we first describe the used technique...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-016-0305-3
更新日期:2017-02-01 00:00:00
abstract::A detailed analysis of the periplasmic electron carriers of the photosynthetic bacterium Ectothiorhodospira sp. has been performed. Two low mid-point redox potential electron carriers, cytochrome c' and cytochrome c, are detected. A high potential iron-sulfur protein is the only high mid-point redox potential electron...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1006486307473
更新日期:2000-01-01 00:00:00
abstract::Temperature response of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) catalytic properties directly determines the CO2 assimilation capacity of photosynthetic organisms as well as their survival in environments with different thermal conditions. Despite unquestionable importance of Rubisco, the comprehensi...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-014-0067-8
更新日期:2015-02-01 00:00:00
abstract::Three Chl-protein complexes were isolated from thylakoid membranes of Bryopsis maxima and Ulva pertusa, marine green algae that inhabit the intertidal zone of the Pacific Ocean off the eastern coast of Japan by dodecyl-beta-D-maltoside polyacrylamide gel electrophoresis. The slowest-moving fractions showed low Chl a/b...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-006-9064-x
更新日期:2006-07-01 00:00:00
abstract::Chlorosomes, the light-harvesting apparatus of green bacteria, are a unique antenna system, in which pigments are organized in aggregates rather than associated with proteins. Isolated chlorosomes from the green sulphur bacterium Chlorobium tepidum contain 10 surface-exposed proteins. Treatment of chlorosomes from Chl...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-005-3757-4
更新日期:2005-11-01 00:00:00
abstract::Oxygen evolving photosystem II particles were exposed to 100 and 250 W m(-2) white light at 20°C under aerobic, anaerobic and strongly reducing (presence of dithionite) conditions. Three types of photoinactivation processes with different kinetics could be distinguished: (1) The fast process which occurs under strongl...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00030061
更新日期:1990-01-01 00:00:00
abstract::In recent years the enzymes of the C3 photosynthetic carbon reduction (PCR) cycle have been studied using the techniques of molecular biology. In this review we discuss the primary protein sequences and structural predictions that have been made for a number of these enzymes, which, with the input of crystallographic ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00029971
更新日期:1991-01-01 00:00:00
abstract::With the discovery of targeted gene replacement, moss biology has been rapidly advancing over the last 10 years. This study demonstrates the usefulness of moss as a model organism for plant photosynthesis research. The two mosses examined in this study, Physcomitrella patens and Ceratodon purpureus, are easily culture...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-004-5577-3
更新日期:2005-01-01 00:00:00
abstract::Solubilisation of thylakoid membranes from young leaves of Pisum sativum in the presence of Triton X-100 resulted in an almost complete loss of quenching of light-harvesting chlorophyll-protein (LHCP) fluorescence, as measured at 77°K. There were concomitant changes in the kinetics of light-saturation curves of electr...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00037130
更新日期:1986-01-01 00:00:00
abstract::A combination of inhibitor and (15)N studies were used to investigate the photorespiratory nitrogen cycle in maize, a C4 plant. Inhibitors used included isonicotinyl hydrazide which blocks the conversion of glycine to serine, methionine sulfoximine an inhibitor of GS and azaserine an inhibitor of GOGAT. Results from l...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00041796
更新日期:1983-01-01 00:00:00
abstract::Roderick K. Clayton passed away on October 23, 2011, at the age of 89, shortly after the plan for this dedicatory issue of Photosynthesis Research had been hatched. I had just written a lengthy letter to him to re-establish contact after a hiatus of 2 or 3 years, and to suggest that I visit him to talk about his life....
journal_title:Photosynthesis research
pub_type: 传,历史文章,杂志文章
doi:10.1007/s11120-013-9948-5
更新日期:2014-05-01 00:00:00
abstract::Structural analogues of the NADP(+) were studied as potential coenzymes and inhibitors for NADP(+) dependent malic enzyme from Zea mays L. leaves. Results showed that 1, N(6)-etheno-nicotinamide adenine dinucleotide phosphate (∈ NADP(+)), 3-acetylpyridine-adenine dinucleotide phosphate (APADP(+)), nicotinamide-hypoxan...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00033716
更新日期:1991-05-01 00:00:00
abstract::Since 1986, non-uniform photosynthesis over the leaf area that may be attributed to patchy stomatal closure, has been an important issue in stress physiology of photosynthesis. In this review, I first outline the gaseous environment within the intercellular spaces, because this is the most fundamental background of th...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00035537
更新日期:1992-03-01 00:00:00
abstract::Electron transfer pathways in photosynthesis involve interactions between membrane-bound complexes such as reaction centres with an extrinsic partner. In this study, the biological specificity of electron transfer between the reaction centre-light-harvesting 1-PufX complex and its extrinsic electron donor, cytochrome ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-013-9812-7
更新日期:2014-05-01 00:00:00
abstract::Fluorescence and electron paramagnetic resonance measurements were used to study selenium influence on photosystem activity in rape seedlings affected by Cd stress. Water cultures containing Hoagland nutrients were supplemented with 400 microM of CdCl(2), 2 microM of Na(2)SeO(4) and a mixture of both CdCl(2) and Na(2)...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-010-9551-y
更新日期:2010-07-01 00:00:00
abstract::A spontaneous mutant (R/89) of photosynthetic purple bacterium Rhodobacter sphaeroides R-26 was selected for resistance to 200 μM atrazin. It showed increased resistance to interquinone electron transfer inhibitors of o-phenanthroline (resistance factor, RF=20) in UQo reconstituted isolated reaction centers and terbut...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00032585
更新日期:1995-08-01 00:00:00
abstract::We investigated the dark-to-light transition in Synechococcus sp. PCC 7942 cells by a detailed analysis of fluorescence transients induced by strong red light. The transients, recorded with high data-acquisition, revealed all the steps of the fast (OJIP; 10(-5)-1 s) and slow phase (PSM(T); 1-10(3) s), kinetically dist...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-009-9405-7
更新日期:2009-03-01 00:00:00
abstract::We have constructed a mutant Synechococcus sp. PCC 7942, termed R2HECAT, in which the entire phycobilisome rod operon has been deleted. In the whole cell absorption spectra of R2HECAT, the peak corresponding to phycocyanin (PC), λmax≈620 nm, could not be detected. However, a single pigment-protein fraction with λmax=6...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00032236
更新日期:1995-07-01 00:00:00
abstract::The thylakoid membrane protein PsbS is critical for quenching excessive excitation energy in mechanisms that involve the light-harvesting complexes of photosystem II. Liposomes of thylakoid lipids have been shown to be a very good platform to study photosynthetic membrane proteins and their interactions. In this study...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-015-0176-z
更新日期:2016-01-01 00:00:00
abstract::Cuttings of grapevine (Vitis vinifera L. cv. Chardonnay) were dark-forced at least three weeks. Pigment contents, 77 K fluorescence emission, excitation spectra of the leaves, petioles, stems, transmission electron micrographs of the etioplasts from leaves, the chlorenchyma tissues of the stems were analysed. The dark...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-004-1061-3
更新日期:2004-01-01 00:00:00
abstract::Moderately high temperature reduces photosynthetic capacities of leaves with large effects on thylakoid reactions of photosynthesis, including xanthophyll conversion in the lipid phase of the thylakoid membrane. In previous studies, we have found that leaf temperature of 40°C increased zeaxanthin accumulation in dark-...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-011-9672-y
更新日期:2011-09-01 00:00:00
abstract::The discovery of a new maize (Zea mays L.) transposon system, Mutator, and the cloning of the 1.4 kilobase transposon, Mul, have made feasible the isolation of nuclear photosynthetic genes which are recognized only by their mutant phenotype. Mutant maize plants which express a high chlorophyll fluorescent (hcf) phenot...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00042979
更新日期:1988-10-01 00:00:00