Time-resolved infrared spectroscopy in the study of photosynthetic systems.

abstract：:Chlorophyll (Chl) is an essential component of the photosynthetic apparatus. Embedded into Chl-binding proteins, Chl molecules play a central role in light harvesting and charge separation within the photosystems. It is critical for the photosynthetic cell to not only ensure the synthesis of a sufficient amount of new...

journal_title：Photosynthesis research

authors： Sobotka R

更新日期：2014-02-01 00:00:00

abstract：:The chlorophyll content is an important experimental parameter in agronomy and plant biology research. In this report, we explore the feasibility of determining total concentration of extracts containing chlorophyll a and chlorophyll b by chlorophyll fluorescence. We found that an excitation at 457 nm results in the s...

journal_title：Photosynthesis research

authors： Lamb JJ,Eaton-Rye JJ,Hohmann-Marriott MF

更新日期：2012-10-01 00:00:00

abstract：:The Fabaceae tree Butea monosperma (TAUB.; syn. Erythrina monosperma (LAM.)) is widely distributed in Central and West-India. We studied it at three sites, i.e. at two locations with contrasting exposure (NE and SW, respectively) in a small mountain range with poor soil on highly drained rocky slopes and at a third lo...

journal_title：Photosynthesis research

authors： Mikosch M,Kumari N,Sharma T,Sharma V,Gessler A,Fischer-Schliebs E,Lüttge U

更新日期：2012-09-01 00:00:00

abstract：:Various techniques of electron microscopy (EM) such as ultrathin sectioning, freeze-fracturing, freeze-etching, negative staining and (cryo-)electron crystallography of two-dimensional crystals have been employed, since now, to obtain much of the structural information of the Photosystem II (PS II) pigment-protein com...

journal_title：Photosynthesis research

authors： Bumba L,Vácha FE

更新日期：2003-01-01 00:00:00

abstract：:The polypeptide composition of thylakoid membranes of the red alga Cyanidium caldarium was studied by PAGE in the presence of lithium dodecyl sulfate. The thylakoid membranes were shown to contain 65 polypeptides with mol wt from 110 to 10 kDa. PS I isolated from C. caldarium cells is composed of at least 5 components...

journal_title：Photosynthesis research

authors： Yurina NP,Karakashev GV,Karapetyan NV,Odintsova MS

更新日期：1991-10-01 00:00:00

abstract：:The 16th International Congress on Photosynthesis will be held August 11-16, 2013 in St. Louis, Missouri, USA. The congress will include 15 plenary lectures, 21 scientific symposia, poster sessions, exhibitors, opening reception, final banquet, excursions and accompanying persons program. The congress is organized as ...

journal_title：Photosynthesis research

authors： Blankenship RE,Musick J,Cooley J,Dutcher S,Govindjee

更新日期：2013-07-01 00:00:00

abstract：:Modified forms of the D1 protein with deletions in lumen-exposed regions, were constructed in the cyanobacterium Synechocystis 6803 using site-directed mutagenesis. Integration and stability of the mutated D1 proteins in the thylakoid membrane were studied by immunoblot and pulse-chase analyses. It was found that in Δ...

journal_title：Photosynthesis research

authors： Salih G,Wiklund R,Tyystjärvi T,Mäenpää P,Gerez C,Jansson C

更新日期：1996-08-01 00:00:00

abstract：:The quantum yield for light-induced H2 generation was measured for a previously optimized bio-hybrid cytochrome c 6-crosslinked PSI(C13G)-1,8-octanedithiol-[FeFe]-H2ase(C97G) (PSI-H2ase) nanoconstruct. The theoretical quantum yield for the PSI-H2ase nanoconstruct is 0.50 molecules of H2 per photon absorbed, which equa...

journal_title：Photosynthesis research

authors： Applegate AM,Lubner CE,Knörzer P,Happe T,Golbeck JH

更新日期：2016-01-01 00:00:00

abstract：:Roderick K. Clayton passed away on October 23, 2011, at the age of 89, shortly after the plan for this dedicatory issue of Photosynthesis Research had been hatched. I had just written a lengthy letter to him to re-establish contact after a hiatus of 2 or 3 years, and to suggest that I visit him to talk about his life....

journal_title：Photosynthesis research

authors： Wraight CA

更新日期：2014-05-01 00:00:00

abstract：:Water vapor was added differentially to the gas streams entering the cells of three makes of differential infrared carbon dioxide analysers. Analyser deflections were compared with those expected from dilution of the carbon dioxide by the additional gas. Tests were made at 0, 365, and 730 cm(3) m(-3) concentrations of...

journal_title：Photosynthesis research

authors： Bunce JA,Ward DA

更新日期：1985-12-01 00:00:00

abstract：:Photoacclimation by strains of Haslea "blue" diatom species H. ostrearia and H. silbo sp. nov. ined. was investigated with rapid light curves and induction-recovery curves using fast repetition rate fluorescence. Cultures were grown to exponential phase under 50 µmol m-2 s-1 photosynthetic available radiation (PAR) an...

journal_title：Photosynthesis research

authors： Perkins R,Williamson C,Lavaud J,Mouget JL,Campbell DA

更新日期：2018-09-01 00:00:00

abstract：:Two portable instruments, designed to evaluate epidermal UV screening in leaves, were compared: the Dualex and the UV-A-PAM fluorimeter. Both instruments excite chlorophyll fluorescence at the same UV wavelengths but reference excitation is in the red and the blue spectral range in the former and the latter fluorimete...

journal_title：Photosynthesis research

authors： Pfündel EE,Ben Ghozlen N,Meyer S,Cerovic ZG

更新日期：2007-07-01 00:00:00

abstract：:The author gives an autobiographical sketch of his path to chlorophyll research, and describes some results. The discussion is largely focused on long wavelength forms of chlorophyll and how they might be generated by self-assembly. Dimers or oligomers, (Chl)n, result from coordination interactions between the central...

journal_title：Photosynthesis research

authors： Katz JJ

更新日期：1990-12-01 00:00:00

abstract：:The activity of ribulose-1,5-bisphosphate carboxylase/oxygenase fromEuglena gracilis decays steadily when exposed to agents that induce oxidative modification of cysteine residues (Cu(2+), benzofuroxan, disulfides, arsenite, oxidized ascorbate). Inactivation takes place with a concomitant loss of cysteine sulfhydryl g...

journal_title：Photosynthesis research

authors： García-Ferris C,Moreno J

更新日期：1993-01-01 00:00:00

abstract：:A pigment system containing carotenoids and oxidised reaction centre pigments is present in chromatophores of Rhodospirillum rubrum and this pigment system may cause fluorescence quenching when a still unidentified chromatophore component is in its oxidised state. Besides by its action spectrum, this pigment system is...

journal_title：Photosynthesis research

authors： Goedheer JC

更新日期：1984-03-01 00:00:00

abstract：:Recycling of carbon by the photorespiratory pathway involves enzymatic steps in the chloroplast, mitochondria, and peroxisomes. Most of these reactions are essential for plants growing under ambient CO(2) concentrations. However, some disruptions of photorespiratory metabolism cause subtle phenotypes in plants grown i...

journal_title：Photosynthesis research

authors： Cousins AB,Walker BJ,Pracharoenwattana I,Smith SM,Badger MR

更新日期：2011-09-01 00:00:00

abstract：:The interaction of PsaC with the core heterodimer of Photosystem 1 was studied in wild type Synechocystis sp. PCC 6803 and the site-directed mutant R561E of PsaB. The mutant reaction center was much less stable in urea and the functional reconstitution of the mutant core using PsaC was impaired. However, the extent of...

journal_title：Photosynthesis research

authors： Rodday SM,Schulz R,McLntosh L,Biggins J

更新日期：1994-12-01 00:00:00

abstract：:Historically, two main concepts guided research into possible mechanisms of light-induced atomic rearrangements in oxygenic photosynthesis: Photodecomposition of CO2 and photodecomposition of water. Both concepts envisioned photoinduced transfers of cumbersome whole atoms and not, as is currently held, photoinduced el...

journal_title：Photosynthesis research

authors： Arnon DI

更新日期：1991-09-01 00:00:00

abstract：:A rapid procedure has been developed for the isolation of the photosystem two reaction centre complex (PS II RC) from a double mutant of Chlamydomonas reinhardtii, F54-14, which lacks the Photosystem one complex and the chloroplast ATPase. Thylakoid membranes are solubilised with 1.5% (w/v) Triton X-100 and the PS II ...

journal_title：Photosynthesis research

authors： Alizadeh S,Nixon PJ,Telfer A,Barber J

更新日期：1995-02-01 00:00:00

abstract：:We have constructed a mutant Synechococcus sp. PCC 7942, termed R2HECAT, in which the entire phycobilisome rod operon has been deleted. In the whole cell absorption spectra of R2HECAT, the peak corresponding to phycocyanin (PC), λmax≈620 nm, could not be detected. However, a single pigment-protein fraction with λmax=6...

journal_title：Photosynthesis research

authors： Bhalerao RP,Gillbro T,Gustafsson P

更新日期：1995-07-01 00:00:00

abstract：:The factors governing quinone protein binding in the photosynthetic bacterium Rps. viridis are investigated via use of empirical intermolecular energy calculations using the GRID method (PJ Goodford (1985) J Med Chem 28: 849-854). A probe atom corresponding to a carbonyl oxygen atom is used to investigate regions exhi...

journal_title：Photosynthesis research

authors： O'Malley PJ,Braithwaite CJ

更新日期：1994-01-01 00:00:00

abstract：:The oxygen flash yield (YO2) and photochemical yield of PS II (ΦPS II) were simultaneously detected in intact Chlorella cells on a bare platinum oxygen rate electrode. The two yields were measured as a function of background irradiance in the steady-state and following a transition from light to darkness. During stead...

journal_title：Photosynthesis research

authors： Prasil O,Kolber Z,Berry JA,Falkowski PG

更新日期：1996-06-01 00:00:00

abstract：:Chloroplasts of a chlorophyll (Chl) b-less barley mutant were solubilized with digitonin and fractionated by polyacrylamide gel electrophoresis with sodium deoxycholate in the running buffer. By this procedure, in contrast to using sodium dodecylsulfate (SDS) for solubilization, a Chl a-protein analogous to the major ...

journal_title：Photosynthesis research

authors： Duranton J,Brown J

更新日期：1987-01-01 00:00:00

abstract：:This review focuses on our recent efforts in synthetic ruthenium-tyrosine-manganese chemistry mimicking the donor side reactions of Photosystem II. Tyrosine and tryptophan residues were linked to ruthenium photosensitizers, which resulted in model complexes for proton-coupled electron transfer from amino acids. A new ...

journal_title：Photosynthesis research

authors： Lomoth R,Magnuson A,Sjödin M,Huang P,Styring S,Hammarström L

更新日期：2006-01-01 00:00:00

abstract：:In this article I review the history of research on the Fenna-Matthews-Olson (FMO) protein with emphasis on my contributions. The FMO protein, which transfers energy from the chlorosome to the reaction center in green sulfur bacteria, was discovered in 1962 and shown to contain bacteriochlorophyll a. From the absorpti...

journal_title：Photosynthesis research

authors： Olson JM

更新日期：2004-01-01 00:00:00

abstract：:A comparative study of photosystem II complexes isolated from tobacco (Nicotiana tabacum L. cv. John William's Broadleaf) which contains normal stacked thylakoid membranes, and from two chlorophyll deficient tobacco mutants (Su/su and Su/su var. Aurea) which have low stacked grana or essentially unstacked thylakoids w...

journal_title：Photosynthesis research

authors： Specht S,Pistorius EK,Schmid GH

更新日期：1987-01-01 00:00:00

abstract：:The effect of temperatures between 15 and 50°C on the light utilization efficiency of leaf net photosynthesis was studied in two C4 grasses and two C3 legumes. In the legumes, light utilization efficiency (μ mol of CO2 fixed per μ Einstein of incident photosynthetic quantum flux) declined linearly with temperature bet...

journal_title：Photosynthesis research

authors： Ludlow MM

更新日期：1981-12-01 00:00:00

abstract：:Energy storage measurements by modulated photothermal radiometry (PTR) were carried out on intact leaves to assess the value of the PTR method for photosynthesis research. In particular, correlations to the redox state of P700 under various conditions were examined. PTR monitors modulated light conversion to heat by s...

journal_title：Photosynthesis research

authors： Malkin S,Schreiber U,Jansen M,Canaani O,Shalgi E,Cahen D

更新日期：1991-08-01 00:00:00

abstract：:Green sulfur bacteria and heliobacteria are strictly anaerobic phototrophs that have homodimeric type 1 reaction center complexes. Within these complexes, highly reducing substances are produced through an initial charge separation followed by electron transfer reactions driven by light energy absorption. In order to ...

journal_title：Photosynthesis research

authors： Azai C,Tsukatani Y,Itoh S,Oh-oka H

更新日期：2010-06-01 00:00:00

abstract：:Light energy harvested by the pigments in Photosystem I (PSI) is used for charge separation in the reaction center (RC), after which the positive charge resides on a special chlorophyll dimer called P700. In studies on the PSI trapping kinetics, P700(+) is usually chemically reduced to re-open the RCs. So far, the inf...

journal_title：Photosynthesis research

authors： Wientjes E,Croce R

更新日期：2012-03-01 00:00:00