Abstract:
:The oxygen flash yield (YO2) and photochemical yield of PS II (ΦPS II) were simultaneously detected in intact Chlorella cells on a bare platinum oxygen rate electrode. The two yields were measured as a function of background irradiance in the steady-state and following a transition from light to darkness. During steady-state illumination at moderate irradiance levels, YO2 and ΦPS II followed each other, suggesting a close coupling between the oxidation of water and QA reduction (Falkowski et al. (1988) Biochim. Biophys. Acta 933: 432-443). Following a light-to-dark transition, however, the relationship between QA reduction and the fraction of PS II reaction centers capable of evolving O2 became temporarily uncoupled. ΦPS II recovered to the preillumination levels within 5-10 s, while the YO2 required up to 60 s to recover under aerobic conditions. The recovery of YO2 was independent of the redox state of QA, but was accompanied by a 30% increase in the functional absorption cross-section of PS II (σPS II). The hysteresis between YO2 and the reduction of QA during the light-to-dark transition was dependent upon the reduction level of the plastoquinone pool and does not appear to be due to a direct radiative charge back-reaction, but rather is a consequence of a transient cyclic electron flow around PS II. The cycle is engaged in vivo only when the plastoquinone pool is reduced. Hence, the plastoquinone pool can act as a clutch that disconnects the oxygen evolution from photochemical charge separation in PS II.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Prasil O,Kolber Z,Berry JA,Falkowski PGdoi
10.1007/BF00029472subject
Has Abstractpub_date
1996-06-01 00:00:00pages
395-410issue
3eissn
0166-8595issn
1573-5079journal_volume
48pub_type
杂志文章abstract::The genome of the purple non-sulphur photosynthetic bacterium Rhodopseudomonas acidophila has been found to contain multiple copies of puc light-harvesting (LH2) peripheral antenna complex genes. Three wild-type isolates each exhibiting dissimilar peripheral antenna complex phenotypes in response to growth at reduced ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00034783
更新日期:1996-09-01 00:00:00
abstract::Free ribulose hisphosphate (RuBP(4-)) rather than its magnesium complex (RuBP-Mg(2-)) was the apparent substrate for spinach ribulose bisphosphate carboxylase/oxygenase. The apparent Km for total RuBP (pH 8.0 at 30° C) increased with increasing Mg(2+) concentrations from 11.6 μM at 13.33 mM Mg(2+) to 32.6 μM at 40.33 ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00041806
更新日期:1983-01-01 00:00:00
abstract::A comparative study of photosystem II complexes isolated from tobacco (Nicotiana tabacum L. cv. John William's Broadleaf) which contains normal stacked thylakoid membranes, and from two chlorophyll deficient tobacco mutants (Su/su and Su/su var. Aurea) which have low stacked grana or essentially unstacked thylakoids w...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00032264
更新日期:1987-01-01 00:00:00
abstract::Several protein-coding genes from land plant chloroplasts have been shown to contain introns. The majority of these introns resemble the fungal mitochondrial group II introns due to considerable nucleotide sequence homology at their 5' and 3' ends and they can readily be folded to form six hairpins characteristic of t...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00039484
更新日期:1988-04-01 00:00:00
abstract::To determine the fluorescence properties of cyanobacterial Photosystem I (PS I) in relatively intact systems, fluorescence emission from 20 to 295 K and polarization at 77 K have been measured from phycobilisomes-less thylakoids of Synechocystis sp. PCC 6803 and a mutant strain lacking Photosystem II (PS II). At 295 K...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00028785
更新日期:1992-02-01 00:00:00
abstract::Taro and cocoyam were grown outdoors in either full sun or under 40% shade. Leaves were tagged as they emerged and the effect of leaf age on net CO2 assimilation rate (A) was determined. The effects of shading on A, transpiration (E), stomatal conductance for CO2 (gc) and H2O (gs), and water use efficiency (WUE) were ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00055064
更新日期:1987-01-01 00:00:00
abstract::The Soret absorption spectra of six synthetic rigid porphyrin dimers whose crystal structures have been determined are simulated using simple exciton theory. The objective is to test the validity of the point dipole and associated approximations; the electronic interaction parameters are thus calculated using data obt...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00048299
更新日期:1989-12-01 00:00:00
abstract::A short list of the twentieth century's dominant figures in photosynthesis would unquestionably include Otto Warburg. One of his many discoveries, the 'bicarbonate effect' remains a lasting puzzle to his heirs in the field. Recent developments in this area of research have renewed interest and call for a re-examinatio...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1020447030191
更新日期:2002-01-01 00:00:00
abstract::A synthetic fluorochromous lipid, rhodaminyl triglyceride (rhodaminyl TG), was intercalated into isolated thylakoid membranes of chloroplasts up to 30 molecules per 100 molecules of chlorophyll. As a result of fluorochrome presence, an absorption band appeared in a yellow-green spectrum region, its intensity being com...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00047079
更新日期:1990-11-01 00:00:00
abstract::Photoacclimation by strains of Haslea "blue" diatom species H. ostrearia and H. silbo sp. nov. ined. was investigated with rapid light curves and induction-recovery curves using fast repetition rate fluorescence. Cultures were grown to exponential phase under 50 µmol m-2 s-1 photosynthetic available radiation (PAR) an...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-018-0508-x
更新日期:2018-09-01 00:00:00
abstract::In the leaf succulent Asclepiad Hoya carnosa (L.) R. Br., CAM photosynthesis occurred under well-watered conditions, as characterized by diurnal gas exchange and changes in titratable acidity. Following 10-12 days of severe water stress, the plants shifted from CAM to a modified CAM-idling mode of metabolism. CAM-idli...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00052124
更新日期:1983-09-01 00:00:00
abstract::The current status of research on the structure, regulation, mechanism and importance of Rubisco activase is reviewed. The activase is now recognized to be a member of the AAA(+) family, whose members participate in macromolecular complexes that perform diverse chaperone-like functions. The conserved nucleotide-bindin...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1022458108678
更新日期:2003-01-01 00:00:00
abstract::Photosynthesis was well-established on the earth at least 3.5 thousand million years ago, and it is widely believed that these ancient organisms had similar metabolic capabilities to modern cyanobacteria. This requires that development of two photosystems and the oxygen evolution capability occurred very early in the...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:
更新日期:1992-01-01 00:00:00
abstract::Joan Mary Anderson, known to most people as Jan, was born on May 12, 1932 in Dunedin, New Zealand. She died on August 28, 2015 in Canberra, Australia. To celebrate her life, we present here a brief biography, some comments on her discoveries in photosynthesis during a career spanning more than half a century, and remi...
journal_title:Photosynthesis research
pub_type: 传,历史文章,杂志文章
doi:10.1007/s11120-016-0287-1
更新日期:2016-08-01 00:00:00
abstract::Heliobacteria are a recently discovered group of anoxygenic phototrophic bacteria, first described in 1983. Heliobacteria contain bacteriochlorophyll g, a pigment unique to species of this group, and synthesize the simplest photosynthetic complexes of all known phototrophs. Also, unlike all other phototrophs, heliobac...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-009-9516-1
更新日期:2010-06-01 00:00:00
abstract::Recently, microalgae have attracted attention as sources of biomass energy. However, fatty acids from the microalgae are mainly unsaturated and show low stability in oxygenated environments, due to oxidation of the double bonds. The branched-chain fatty acid, 10-methyl stearic acid, is synthesized from oleic acid in c...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-018-0537-5
更新日期:2019-03-01 00:00:00
abstract::Ongoing work has led to the identification of most of the biochemical steps in carotenoid biosynthesis in chlorophototrophic bacteria. In carotenogenesis, a relatively small number of modifications leads to a great diversity of carotenoid structures. This review examines the individual steps in the pathway, discusses ...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-008-9312-3
更新日期:2008-08-01 00:00:00
abstract::Modulation of the efficiency with which leaves convert absorbed light to photochemical energy [intrinsic efficiency of open photosystem II (PSII) centers, as the ratio of variable to maximal chlorophyll fluorescence] as well as leaf xanthophyll composition (interconversions of the xanthophyll cycle pigments violaxanth...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-012-9761-6
更新日期:2012-09-01 00:00:00
abstract::The effect of nitrate and nitrite on long-term chlorophyll fluorescence has been studied in filamentous blue-green algae. Cells grown autotrophically with nitrate as nitrogen source show, under argon atmosphere, a high level of fluorescence. The addition of either nitrete or nitrite induces a significant fluorescence ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00032356
更新日期:1981-09-01 00:00:00
abstract::A new binding site for anions which inhibit the water oxidizing complex (WOC) of Photosystem II in spinach has been identified. Anions which bind to this site inhibit the flash-induced S2/S0 catalase reaction (2H2O2→2H2O+O2) of the WOC by displacing hydrogen peroxide. Using a mass spectrometer and gas permeable membra...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00046771
更新日期:1993-01-01 00:00:00
abstract::When excited by ultraviolet radiation, leaves of a great number of species of higher plants exhibit emission of blue fluorescence, comparable in intensity to the red emission of chlorophyll. The fluorescence decay of the blue emission of spinach leaves recorded by single photon counting techniques is decomposed into e...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00033171
更新日期:1990-09-01 00:00:00
abstract::A protocol for the isolation of functional thylakoids from Arabidopsis thaliana leaves was developed. The critical factor in obtaining active, coupled and stable preparation is the inclusion of EDTA and EGTA in the grinding buffer. Preparations were characterized with respect to the whole or partial electron transport...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1011818021875
更新日期:2001-01-01 00:00:00
abstract::The biogenesis and oxygen-evolving activity of cyanobacterial Photosystem II (PSII) is dependent on a number of accessory proteins not found in the crystallised dimeric complex. These include Psb27, a small lipoprotein attached to the lumenal side of PSII, which has been assigned a role in regulating the assembly of t...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-011-9712-7
更新日期:2012-02-01 00:00:00
abstract::Here, we describe a new imaging setup able to assess in vivo photosynthetic activity. The system specifically measures time-resolved chlorophyll fluorescence in response to light. It is composed of a fast digital camera equipped with a wide-angle lens for the analysis of samples up to 10 x 10 cm, i.e. entire plants or...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-009-9487-2
更新日期:2009-10-01 00:00:00
abstract::In order to evaluate genotypic variation, we measured leaf photosynthetic rate (Pn) and its associated factors for the rice diversity research set of germplasm (RDRS) selected from the Genebank in National Institute of Agrobiological Sciences (NIAS). Pn showed large genotypic variation from 11.9 to 32.1 micromol m(-2 ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-007-9208-7
更新日期:2007-10-01 00:00:00
abstract::Chlorosomes of green photosynthetic bacterium Chlorobium tepidum contain aggregates of bacteriochlorophyll c (BChl c) with carotenoids and isoprenoid quinones. BChl aggregates with very similar optical properties can be prepared also in vitro either in non-polar solvents or in aqueous buffers with addition of lipids a...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-007-9259-9
更新日期:2008-02-01 00:00:00
abstract::C4 photosynthesis is a complex trait that has a high degree of natural variation, involving anatomical and biochemical changes relative to the ancestral C3 state. It has evolved at least 66 times across a variety of lineages and the evolutionary route from C3 to C4 is likely conserved but not necessarily genetically i...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-013-9872-8
更新日期:2014-02-01 00:00:00
abstract::Phosphoenolpyruvate carboxylase from leaves of the C4 plant Setaria verticillata (L.) Beauv. is activated by light; day levels of activity are reached after 30 minutes of illumination. Photoactivation is prevented by inhibitors of photosynthetic electron flow or of photophosphorylation and by D,L-glyceraldehyde, which...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00028842
更新日期:1988-06-01 00:00:00
abstract::Intra- and inter-specific variation in CO2 assimilation rate (A) in Triticum spp. is well documented for reproductive growth stages. Research was conducted to characterize early vegetative photosynthetic variation in a diverse set of cultivated hexaploid wheat (T. aestivum L.) germplasm and in wild tetraploid (T. dico...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00034120
更新日期:1989-05-01 00:00:00
abstract::Chlorophyll f is a photosynthetic pigment that was discovered in 2010. In this study, we present investigations on spectral and dynamic characteristics of singlet-excited and triplet states of Chl f with the application of ultrafast time-resolved absorption and fluorescence spectroscopies. The pigment was studied at r...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-014-9981-z
更新日期:2014-07-01 00:00:00