Abstract:
:The chlorophyll content is an important experimental parameter in agronomy and plant biology research. In this report, we explore the feasibility of determining total concentration of extracts containing chlorophyll a and chlorophyll b by chlorophyll fluorescence. We found that an excitation at 457 nm results in the same integrated fluorescence emission for a molecule of chlorophyll a and a molecule of chlorophyll b. The fluorescence yield induced by 457 nm is therefore proportional to total molar chlorophyll concentration. Based on this observation, we designed an instrument to determine total chlorophyll concentrations. A single light emitting diode (LED) is used to excite chlorophyll extracts. After passing through a long-pass filter, the fluorescence emission is assessed by a photodiode. We demonstrate that this instrument facilitates the determination of total chlorophyll concentrations. We further extended the functionality of the instrument by including LEDs emitting at 435 and 470 nm wavelengths, thereby preferentially exciting chlorophyll a and chlorophyll b. This instrument can be used to determine chlorophyll a and chlorophyll b concentrations in a variety of organisms containing different ratios of chlorophylls. Monte-Carlo simulations are in agreement with experimental data such that a precise determination of chlorophyll concentrations in carotenoid-containing biological samples containing a concentration of less than 5 nmol/mL total chlorophyll can be achieved.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Lamb JJ,Eaton-Rye JJ,Hohmann-Marriott MFdoi
10.1007/s11120-012-9777-ysubject
Has Abstractpub_date
2012-10-01 00:00:00pages
59-68issue
1eissn
0166-8595issn
1573-5079journal_volume
114pub_type
杂志文章abstract::Fourier transform infrared (FTIR) spectroscopy probes the vibrational properties of amino acids and cofactors, which are sensitive to minute structural changes. The lack of specificity of this technique, on the one hand, permits us to probe directly the vibrational properties of almost all the cofactors, amino acid si...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-009-9439-x
更新日期:2009-08-01 00:00:00
abstract::The unicellular green alga Chlamydomonas reinhardtii acclimates to low-CO2 (LC) conditions by actively transporting inorganic carbon (Ci) into the cell, resulting in an increase in photosynthetic efficiency. This mechanism is called the carbon-concentrating mechanism (CCM), and soluble protein LCIB is essential for th...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-013-9963-6
更新日期:2014-09-01 00:00:00
abstract::Although it has been believed that wild-type plants are capable of protecting photosystem I (PSI) under high light, our previous study indicates that PSI is sensitive to high light in the shade-established tree species Psychotria rubra. However, the underlying physiological mechanisms are unclear. In this study, we ex...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-016-0275-5
更新日期:2016-07-01 00:00:00
abstract::We generated transgenic tobacco plants with high levels of fructose-1,6-bisphosphatase expressing cyanobacterialfructose-1,6-/sedoheptulose-1,7-bisphosphatase in the cytosol. At ambient CO(2) levels (360 ppm), growth, photosynthetic activity, and fresh weight were unchanged but the sucrose/hexose/starch ratio was slig...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-011-9645-1
更新日期:2011-05-01 00:00:00
abstract::Chlorosomes of green photosynthetic bacterium Chlorobium tepidum contain aggregates of bacteriochlorophyll c (BChl c) with carotenoids and isoprenoid quinones. BChl aggregates with very similar optical properties can be prepared also in vitro either in non-polar solvents or in aqueous buffers with addition of lipids a...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-007-9259-9
更新日期:2008-02-01 00:00:00
abstract::The effect of leaf water potential (Ψ) on net CO2 assimilation rate (A), stomatal conductance (g), transpiration (E) and water-use efficiency (WUE) was measured for three cultivars of cacao (Theobroma cacao L.) seedlings during three recurrent drought cycles. Net assimilation varied greatly at high water potentials, b...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00037179
更新日期:1989-09-01 00:00:00
abstract::Light activation of phosphoenolpyruvate carboxylase from the leaves of the C4 plant Setaria verticillata (L.) is more pronounced at low CO2 levels. The 2-fold activation observed at physiological ambient CO2 becomes 3.64-fold at 5 μL/L and completely abolished above 700 μL/L. When the stomata close under the influence...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00034834
更新日期:1988-11-01 00:00:00
abstract::Pulse modulated fluorescence has increasingly been used as an ecological tool to examine changes in the vertical distribution of microphytobenthic cells within the upper layers of estuarine sediments (most often using the minimum fluorescence yield F(o)) as well as to indicate the health of the community (using the ma...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/B:PRES.0000028397.86495.b5
更新日期:2004-01-01 00:00:00
abstract::The engagement of different photoprotective mechanisms in the cells of the carotenogenic astaxanthin-accumulating chlorophyte Haematococcus pluvialis (i) under favorable conditions, (ii) in the course of stress-induced haematocyst formation and (iii) during recovery from the stress was studied. To this end, we followe...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-016-0246-x
更新日期:2016-06-01 00:00:00
abstract::Green sulfur bacteria and heliobacteria are strictly anaerobic phototrophs that have homodimeric type 1 reaction center complexes. Within these complexes, highly reducing substances are produced through an initial charge separation followed by electron transfer reactions driven by light energy absorption. In order to ...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-009-9521-4
更新日期:2010-06-01 00:00:00
abstract::DCMU (3-(3,4-dichlorophenyl)-1,1-dimethylurea) at concentrations higher than 10 μM suppresses the second time range delayed fluorescence (DF) of pea chloroplasts, due to inhibition of the oxidizing side of photosystem II (PS II). The inhibition of the reducing side of PS II resulting in the suppression of millisecond ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00054986
更新日期:1988-01-01 00:00:00
abstract::The seasonal maximum in photosynthetic CO2 exchange rate (CER) and the cessation of leaf expansion in soybeans (Glycine max (L.) Merr) accompany fruiting under normal agricultural conditions. To investigate whether these phenomena were obligatively tied together, we caused early flowering of long-season varieties by i...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00034108
更新日期:1982-12-01 00:00:00
abstract::In polyploid plants the photosynthetic rate per cell is correlated with the amount of DNA per cell. The photosynthetic rate per unit leaf area is the product of the rate per cell times the number of photosynthetic cells per unit area. Therefore, the photosynthetic rate per unit leaf area will increase if there is a le...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00014744
更新日期:1993-02-01 00:00:00
abstract::A correlated influence of cation concentration and excitation energy level on PS II Abbreviations: AMPD = 2-amino-2-methyl-1,3-propanediol; DCIP = 2-6 dichlorophenol indophenol; DCIPH2 = DCIP in reduced state; DCIPr = rate of DCIP-reduction; DCMU = 3-(3,4 dichlorophenyl)1-1 dimethyl-urea; Fv = variable fluorescence; F...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00018228
更新日期:1980-06-01 00:00:00
abstract::A protocol for the isolation of functional thylakoids from Arabidopsis thaliana leaves was developed. The critical factor in obtaining active, coupled and stable preparation is the inclusion of EDTA and EGTA in the grinding buffer. Preparations were characterized with respect to the whole or partial electron transport...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1011818021875
更新日期:2001-01-01 00:00:00
abstract::Roderick K. Clayton passed away on October 23, 2011, at the age of 89, shortly after the plan for this dedicatory issue of Photosynthesis Research had been hatched. I had just written a lengthy letter to him to re-establish contact after a hiatus of 2 or 3 years, and to suggest that I visit him to talk about his life....
journal_title:Photosynthesis research
pub_type: 传,历史文章,杂志文章
doi:10.1007/s11120-013-9948-5
更新日期:2014-05-01 00:00:00
abstract::The quantum yield for light-induced H2 generation was measured for a previously optimized bio-hybrid cytochrome c 6-crosslinked PSI(C13G)-1,8-octanedithiol-[FeFe]-H2ase(C97G) (PSI-H2ase) nanoconstruct. The theoretical quantum yield for the PSI-H2ase nanoconstruct is 0.50 molecules of H2 per photon absorbed, which equa...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-014-0064-y
更新日期:2016-01-01 00:00:00
abstract::Several protein-coding genes from land plant chloroplasts have been shown to contain introns. The majority of these introns resemble the fungal mitochondrial group II introns due to considerable nucleotide sequence homology at their 5' and 3' ends and they can readily be folded to form six hairpins characteristic of t...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00039484
更新日期:1988-04-01 00:00:00
abstract::The polypeptide composition of thylakoid membranes of the red alga Cyanidium caldarium was studied by PAGE in the presence of lithium dodecyl sulfate. The thylakoid membranes were shown to contain 65 polypeptides with mol wt from 110 to 10 kDa. PS I isolated from C. caldarium cells is composed of at least 5 components...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00035678
更新日期:1991-10-01 00:00:00
abstract::The crystallization of a given protein is a hard task being even more complicated when the protein shows a hydrophobic behavior. In the case of photosynthetic proteins, the difficulty of the experiments increased due to the high light sensitivity. Aqueous solutions of photosystem II core complex (OEC PSII) of Pisum sa...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-007-9131-y
更新日期:2006-12-01 00:00:00
abstract::In plants, chlorophylls (chlorophyll a and chlorophyll b) are the most abundant tetrapyrrole molecules and are essential for photosynthesis. The first committed step of chlorophyll biosynthesis is the insertion of Mg(2+) into protoporphyrin IX, and thus subsequent steps of the biosynthesis are called the Mg branch. As...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-008-9291-4
更新日期:2008-05-01 00:00:00
abstract::The rate and extent of light activation of PEPC may be used as another criterion to distinguish C3 and C4 plants. Light stimulated phosphoenolypyruvate carboxylase (PEPC) in leaf discs of C4 plants, the activity being three times greater than that in the dark but stimulation of PEPC was limited about 30% over the dark...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00015061
更新日期:1993-10-01 00:00:00
abstract::The role of the ferredoxin:thioredoxin system in the reversible light activation of chloroplast enzymes by thiol-disulfide interchange with thioredoxins is now well established. Recent fruitful collaboration between biochemists and structural biologists, reflected by the shared authorship of the paper, allowed to solv...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/B:PRES.0000017194.34167.6d
更新日期:2004-01-01 00:00:00
abstract::The chloroplast photo-oxidation and the expression of the Cab gene Lhcb1, encoding the Lhcb1 light-harvesting chlorophyll a/b-protein of PS II, have been studied in leaf cells of maize treated with the two bleaching herbicides norflurazon and amitrole and of the two carotenoid-free mutants vp9 and vp2 grown under high...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1006477215572
更新日期:2000-01-01 00:00:00
abstract::Photosynthetic organisms normally endeavor to optimize the efficiency of their light-harvesting apparatus. However, here we describe two bacterial isolates belonging to the genera Allochromatium and Thiocapsa that demonstrate a novel adaptation by optimizing their external growth conditions at the expense of photosynt...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-007-9246-1
更新日期:2008-02-01 00:00:00
abstract::The current status of research on the structure, regulation, mechanism and importance of Rubisco activase is reviewed. The activase is now recognized to be a member of the AAA(+) family, whose members participate in macromolecular complexes that perform diverse chaperone-like functions. The conserved nucleotide-bindin...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1022458108678
更新日期:2003-01-01 00:00:00
abstract::A protein was isolated from membranes of the green sulfur bacterium Chlorobium tepidum. This protein was characterized by gel electrophoresis, gel filtration, analytical ultracentrifugation and amino acid sequencing. The molecular weight of the purified protein was shown to be 26 kDa by SDS-PAGE. HPLC gelfiltration, S...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/B:PRES.0000015383.58680.56
更新日期:2004-02-01 00:00:00
abstract::Brownian Dynamics (BD) computer simulations were used to study electrostatic interactions between turnip cytochrome f (cyt f) and spinach plastocyanin (PC). Three different spinach PC structures were studied: The X-ray crystal structure of Xue and coworkers [(1998) Protein Sci 7:2099-2105] and the NMR structure of Mus...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-007-9192-y
更新日期:2007-11-01 00:00:00
abstract::The three major chlorophyll-proteins of spinach chloroplasts were solubilized with digitonin and isolated by electrophoresis with deoxycholate. The gel bands were identified from their absorption and fluorescence spectra measured at 77 K. The slowest moving band was a Photosystem I complex (CPI); the second, a Photosy...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00054145
更新日期:1983-12-01 00:00:00
abstract::Cyanobacteria exhibit a novel form of non-photochemical quenching (NPQ) at the level of the phycobilisome. NPQ is a process that protects photosystem II (PSII) from possible highlight-induced photo-damage. Although significant advancement has been made in understanding the NPQ, there are still some missing details. Th...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-018-0498-8
更新日期:2018-08-01 00:00:00