Isolation and Characterization of an Outer Membrane Protein of Chlorobium tepidum.

Abstract:

:A protein was isolated from membranes of the green sulfur bacterium Chlorobium tepidum. This protein was characterized by gel electrophoresis, gel filtration, analytical ultracentrifugation and amino acid sequencing. The molecular weight of the purified protein was shown to be 26 kDa by SDS-PAGE. HPLC gelfiltration, SDS-PAGE and analytical ultracentrifugation are consistent with the presence of a homogenous protein in the preparations. Amino acid analysis was obtained from the isolated protein after fragmentation with Lys-C, trypsin and cyanogen bromide. The cleavage pattern resulting from these treatments combined with Edman sequencing yield a sequence allowing the identification of an integral membrane agglutinin in Chl. tepidum.

journal_name

Photosynth Res

journal_title

Photosynthesis research

authors

Aivaliotis M,Neofotistou E,Rémigy HW,Tsimpinos G,Lustig A,Lottspeich F,Tsiotis G

doi

10.1023/B:PRES.0000015383.58680.56

subject

Has Abstract

pub_date

2004-02-01 00:00:00

pages

161-6

issue

2

eissn

0166-8595

issn

1573-5079

pii

10.1023/B:PRES.0000015383.58680.56

journal_volume

79

pub_type

杂志文章
  • Analogues of NADP(+) as inhibitors and coenzymes for NADP(+) malic enzyme from maize leaves.

    abstract::Structural analogues of the NADP(+) were studied as potential coenzymes and inhibitors for NADP(+) dependent malic enzyme from Zea mays L. leaves. Results showed that 1, N(6)-etheno-nicotinamide adenine dinucleotide phosphate (∈ NADP(+)), 3-acetylpyridine-adenine dinucleotide phosphate (APADP(+)), nicotinamide-hypoxan...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/BF00033716

    authors: Spampinato CP,Paneth P,O'Leary MH,Andreo CS

    更新日期:1991-05-01 00:00:00

  • Far-red light photoacclimation (FaRLiP) in Synechococcus sp. PCC 7335: I. Regulation of FaRLiP gene expression.

    abstract::Far-red light photoacclimation (FaRLiP) is a mechanism that allows some cyanobacteria to utilize far-red light (FRL) for oxygenic photosynthesis. During FaRLiP, cyanobacteria remodel photosystem (PS) I, PS II, and phycobilisomes while synthesizing Chl d, Chl f, and far-red-absorbing phycobiliproteins, and these change...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/s11120-016-0309-z

    authors: Ho MY,Gan F,Shen G,Zhao C,Bryant DA

    更新日期:2017-02-01 00:00:00

  • Phototrophic purple sulfur bacteria as heat engines in the South Andros Black Hole.

    abstract::Photosynthetic organisms normally endeavor to optimize the efficiency of their light-harvesting apparatus. However, here we describe two bacterial isolates belonging to the genera Allochromatium and Thiocapsa that demonstrate a novel adaptation by optimizing their external growth conditions at the expense of photosynt...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/s11120-007-9246-1

    authors: Herbert RA,Gall A,Maoka T,Cogdell RJ,Robert B,Takaichi S,Schwabe S

    更新日期:2008-02-01 00:00:00

  • Modulation of photosynthetic energy conversion efficiency in nature: from seconds to seasons.

    abstract::Modulation of the efficiency with which leaves convert absorbed light to photochemical energy [intrinsic efficiency of open photosystem II (PSII) centers, as the ratio of variable to maximal chlorophyll fluorescence] as well as leaf xanthophyll composition (interconversions of the xanthophyll cycle pigments violaxanth...

    journal_title:Photosynthesis research

    pub_type: 杂志文章,评审

    doi:10.1007/s11120-012-9761-6

    authors: Demmig-Adams B,Cohu CM,Muller O,Adams WW 3rd

    更新日期:2012-09-01 00:00:00

  • An LED-based fluorometer for chlorophyll quantification in the laboratory and in the field.

    abstract::The chlorophyll content is an important experimental parameter in agronomy and plant biology research. In this report, we explore the feasibility of determining total concentration of extracts containing chlorophyll a and chlorophyll b by chlorophyll fluorescence. We found that an excitation at 457 nm results in the s...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/s11120-012-9777-y

    authors: Lamb JJ,Eaton-Rye JJ,Hohmann-Marriott MF

    更新日期:2012-10-01 00:00:00

  • Simultaneous collection of rapid chlorophyll fluorescence induction kinetics, fluorescence quenching parameters, and environmental data using an automated PAM-2000/CR10X data logging system.

    abstract::The PAM-2000 portable chlorophyll fluorometer represents one of the first commercially available instruments utilizing the Pulse Amplitude Modulation (PAM) measurement principle, and has become a widely used platform for measuring chlorophyll fluorescence in a wide range of study systems. In this paper, we describe a ...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/s11120-005-9010-3

    authors: Gray DW,Cardon ZG,Lewis LA

    更新日期:2006-03-01 00:00:00

  • The effects of moderately high temperature on zeaxanthin accumulation and decay.

    abstract::Moderately high temperature reduces photosynthetic capacities of leaves with large effects on thylakoid reactions of photosynthesis, including xanthophyll conversion in the lipid phase of the thylakoid membrane. In previous studies, we have found that leaf temperature of 40°C increased zeaxanthin accumulation in dark-...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/s11120-011-9672-y

    authors: Zhang R,Kramer DM,Cruz JA,Struck KR,Sharkey TD

    更新日期:2011-09-01 00:00:00

  • Quantum yield measurements of light-induced H₂ generation in a photosystem I-[FeFe]-H₂ase nanoconstruct.

    abstract::The quantum yield for light-induced H2 generation was measured for a previously optimized bio-hybrid cytochrome c 6-crosslinked PSI(C13G)-1,8-octanedithiol-[FeFe]-H2ase(C97G) (PSI-H2ase) nanoconstruct. The theoretical quantum yield for the PSI-H2ase nanoconstruct is 0.50 molecules of H2 per photon absorbed, which equa...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/s11120-014-0064-y

    authors: Applegate AM,Lubner CE,Knörzer P,Happe T,Golbeck JH

    更新日期:2016-01-01 00:00:00

  • Engineering cyanobacteria as photosynthetic feedstock factories.

    abstract::Carbohydrate feedstocks are at the root of bioindustrial production and are needed in greater quantities than ever due to increased prioritization of renewable fuels with reduced carbon footprints. Cyanobacteria possess a number of features that make them well suited as an alternative feedstock crop in comparison to t...

    journal_title:Photosynthesis research

    pub_type: 杂志文章,评审

    doi:10.1007/s11120-014-9980-0

    authors: Hays SG,Ducat DC

    更新日期:2015-03-01 00:00:00

  • Artificial inhibitory effects of sulfite on photosystem II activity measured by oxygen evolution in chloroplasts.

    abstract::In this report we demonstrate sulfite interaction with oxygen and PSII electron acceptors (ferricyanide and para-benzoquinone) during measurement of oxygen evolution in chloroplasts. Redox potentials of oxygen, ferricyanide and para-benzoquinone allow them to compete for sulfite. Without taking this into account, sulf...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/BF00019595

    authors: Beauregard M,Popovic R

    更新日期:1987-01-01 00:00:00

  • Mimicking the electron donor side of Photosystem II in artificial photosynthesis.

    abstract::This review focuses on our recent efforts in synthetic ruthenium-tyrosine-manganese chemistry mimicking the donor side reactions of Photosystem II. Tyrosine and tryptophan residues were linked to ruthenium photosensitizers, which resulted in model complexes for proton-coupled electron transfer from amino acids. A new ...

    journal_title:Photosynthesis research

    pub_type: 杂志文章,评审

    doi:10.1007/s11120-005-9005-0

    authors: Lomoth R,Magnuson A,Sjödin M,Huang P,Styring S,Hammarström L

    更新日期:2006-01-01 00:00:00

  • Fourier transform infrared (FTIR) spectroscopy.

    abstract::Fourier transform infrared (FTIR) spectroscopy probes the vibrational properties of amino acids and cofactors, which are sensitive to minute structural changes. The lack of specificity of this technique, on the one hand, permits us to probe directly the vibrational properties of almost all the cofactors, amino acid si...

    journal_title:Photosynthesis research

    pub_type: 杂志文章,评审

    doi:10.1007/s11120-009-9439-x

    authors: Berthomieu C,Hienerwadel R

    更新日期:2009-08-01 00:00:00

  • Regulation and function of xanthophyll cycle-dependent photoprotection in algae.

    abstract::The xanthophyll cycle represents one of the important photoprotection mechanisms in plant cells. In the present review, we summarize current knowledge about the violaxanthin cycle of vascular plants, green and brown algae, and the diadinoxanthin cycle of the algal classes Bacillariophyceae, Xanthophyceae, Haptophyceae...

    journal_title:Photosynthesis research

    pub_type: 杂志文章,评审

    doi:10.1007/s11120-010-9536-x

    authors: Goss R,Jakob T

    更新日期:2010-11-01 00:00:00

  • Growth, photosynthesis and photorespiration of Lemna gibba: response to variations in CO2 and O 2 concentrations and photon flux density.

    abstract::Dry weight and Relative Growth Rate of Lemna gibba were significantly increased by CO2 enrichment up to 6000 μl CO2 l(-1). This high CO2 optimum for growth is probably due to the presence of nonfunctional stomata. The response to high CO2 was less or absent following four days growth in 2% O2. The Leaf Area Ratio decr...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/BF00029048

    authors: Andersen IH,Dons C,Nilsen S,Haugstad MK

    更新日期:1985-03-01 00:00:00

  • Effects of temperature and photosynthetic inhibitors on light activation of C4-phosphoenolpyruvate carboxylase.

    abstract::Phosphoenolpyruvate carboxylase from leaves of the C4 plant Setaria verticillata (L.) Beauv. is activated by light; day levels of activity are reached after 30 minutes of illumination. Photoactivation is prevented by inhibitors of photosynthetic electron flow or of photophosphorylation and by D,L-glyceraldehyde, which...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/BF00028842

    authors: Samaras Y,Manetas Y,Gavalas NA

    更新日期:1988-06-01 00:00:00

  • Electron transfer in photosystem II.

    abstract::The picture presently emerging from studies on the mechanism of photosystem II electron transport is discussed. The reactions involved in excitation trapping, charge separation and stabilization of the charge pair in the reaction center, followed by the reactions with the substrates, plastoquinone reduction and water ...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/BF00032785

    authors: Van Gorkom HJ

    更新日期:1985-01-01 00:00:00

  • The MoD-QM/MM methodology for structural refinement of photosystem II and other biological macromolecules.

    abstract::Quantum mechanics/molecular mechanics (QM/MM) hybrid methods are currently the most powerful computational tools for studies of structure/function relations and structural refinement of macrobiomolecules (e.g., proteins and nucleic acids). These methods are highly efficient, since they implement quantum chemistry tech...

    journal_title:Photosynthesis research

    pub_type: 杂志文章,评审

    doi:10.1007/s11120-009-9467-6

    authors: Sproviero EM,Newcomer MB,Gascón JA,Batista ER,Brudvig GW,Batista VS

    更新日期:2009-11-01 00:00:00

  • The role of inorganic phosphate in the regulation of C4 photosynthesis.

    abstract::Inorganic phosphate participates in many fundamental processes within the plant cell. Its broad influence on plant metabolism is related to such key operations as metabolite transport, enzyme regulation and carbohydrate metabolism in general. This review discusses these topics with special emphasis on the role assigne...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/BF00016551

    authors: Iglesias AA,Plaxton WC,Podestá FE

    更新日期:1993-03-01 00:00:00

  • Fluorescence F 0 of photosystems II and I in developing C3 and C 4 leaves, and implications on regulation of excitation balance.

    abstract::This work addresses the question of occurrence and function of photosystem II (PSII) in bundle sheath (BS) cells of leaves possessing NADP-malic enzyme-type C4 photosynthesis (Zea mays). Although no requirement for PSII activity in the BS has been established, several component proteins of PSII have been detected in B...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/s11120-014-0009-5

    authors: Peterson RB,Oja V,Eichelmann H,Bichele I,Dall'Osto L,Laisk A

    更新日期:2014-10-01 00:00:00

  • Rubisco activase - Rubisco's catalytic chaperone.

    abstract::The current status of research on the structure, regulation, mechanism and importance of Rubisco activase is reviewed. The activase is now recognized to be a member of the AAA(+) family, whose members participate in macromolecular complexes that perform diverse chaperone-like functions. The conserved nucleotide-bindin...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1023/A:1022458108678

    authors: Portis AR Jr

    更新日期:2003-01-01 00:00:00

  • Remembering Otto Kandler (1920-2017) and his contributions.

    abstract::After a brief prologue on Otto Kandler's life, we describe briefly his pioneering work on photosynthesis (photophosphorylation and the carbon cycle) and his key participation in the discovery of the concept of three forms of life (Archaea, Prokarya, and Eukarya). With Otto Kandler's passing, both the international pho...

    journal_title:Photosynthesis research

    pub_type: 传,历史文章,杂志文章

    doi:10.1007/s11120-018-0530-z

    authors: Govindjee,Tanner W

    更新日期:2018-09-01 00:00:00

  • Characterization of a covalently linked complex involving ferredoxin and ferredoxin: NADP reductase.

    abstract::Ferredoxin and the flavoprotein, ferredoxin: NADP reductase, have been covalently linked by incubation in the presence of a water soluble carbodiimide. The cross-linking reaction yields an adduct having a 1:1 stoichiometry. The adduct has depressed levels of diaphorase and NADPH oxidase activity and is inactive in red...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/BF00035450

    authors: Colvert KK,Davis DJ

    更新日期:1988-09-01 00:00:00

  • Contribution to the structural characterization of eucaryotic PSI reaction centre-I. Critical analysis of the polypeptidic composition of different P700 enriched fractions.

    abstract::In thylakoid membranes, several peptides of high MW† are present which may interfere with the study of CP1's components. Modifying Cleveland's technique [7] for limited proteolysis, we have characterized the polypeptides found in the 60 kD region. Some may result from incomplete washing of the CF1 while others come fr...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/BF00020071

    authors: Lagoutte B,Setif P,Duranton J

    更新日期:1980-03-01 00:00:00

  • A three-dimensional model of the Photosystem II reaction centre of Pisum sativum.

    abstract::A three-dimensional model of the core proteins D1 and D2, including the cofactors, that form the Photosystem II reaction centre of pea (Pisum sativum), has been generated. This model was built with a rule-based computer modelling system using the information from the crystal structures of the photosynthetic reaction c...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/BF00033446

    authors: Ruffle SV,Donnelly D,Blundell TL,Nugent JH

    更新日期:1992-11-01 00:00:00

  • Response of chlorophyll d-containing cyanobacterium Acaryochloris marina to UV and visible irradiations.

    abstract::We have previously investigated the response mechanisms of photosystem II complexes from spinach to strong UV and visible irradiations (Wei et al J Photochem Photobiol B 104:118-125, 2011). In this work, we extend our study to the effects of strong light on the unusual cyanobacterium Acaryochloris marina, which is abl...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/s11120-013-9946-7

    authors: Hou X,Raposo A,Hou HJ

    更新日期:2013-11-01 00:00:00

  • The oldest records of photosynthesis.

    abstract::There is diverse, yet controversial fossil evidence for the existence of photosynthesis 3500 million years ago. Among the most persuasive evidence is the stromatolites described from low grade metasedimentary rocks in Western Australia and South Africa. Based on the understanding of the paleobiology of stromatolites a...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/BF00039172

    authors: Awramik SM

    更新日期:1992-08-01 00:00:00

  • In vitro effects of monoterpenes on chloroplast membranes.

    abstract::A chloroplast preparation was extracted from squash (Cucurbita pepo (L.) var. Senator). Enrichment of intact chloroplasts was achieved by continuous free-flow electrophoresis. The addition of monoterpenes, detergent and free fatty acids changed the elecrophoretic separation pattern characteristically. Monoterpene-depe...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/BF00054124

    authors: Klingler H,Frosch S,Wagner E

    更新日期:1991-06-01 00:00:00

  • Afterglow thermoluminescence band as a possible early indicator of changes in the photosynthetic electron transport in leaves.

    abstract::The afterglow (AG) band of thermoluminescence (TL) has been investigated in leaves of Arabidopsis thaliana. Excitation of dark-adapted leaves with two saturating single turn-over flashes induced the appearance of a complex TL glow curve that could be well simulated by three components: the two components, B1 and B2, o...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/s11120-004-7311-6

    authors: Roncel M,Ortega JM

    更新日期:2005-06-01 00:00:00

  • Spectral and kinetic characterization of electron acceptor A1 in a Photosystem I core devoid of iron-sulfur centers F X, F B and F A.

    abstract::The kinetic and spectroscopic properties of the secondary electron acceptor A1 were determined by flash absorption spectroscopy at room and cryogenic temperatures in a Photosystem I (PS I) core devoid of the iron-sulfur clusters FX, FB and FA. It was shown earlier (Warren, P.V., Golbeck, J.H. and Warden, J.T. (1993) B...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/BF00015559

    authors: Brettel K,Golbeck JH

    更新日期:1995-09-01 00:00:00

  • Effect of light intensity on ammonia assimilation in maize leaves.

    abstract::The effect of light on the metabolism of ammonia was studied by subjecting detached maize leaves to 150 or 1350 μmol m(-2) s(-1) PAR during incubation with the leaf base in 2 mM (15)NH4Cl. After up to 60 min, leaves were extracted. Ammonia, glutamine, glycine, serine, alanine, and aspartate were separated by isotherma...

    journal_title:Photosynthesis research

    pub_type: 杂志文章

    doi:10.1007/BF00049278

    authors: Klaus RE,Berger MG,Fock HP

    更新日期:1985-09-01 00:00:00