Abstract:
:The quantum yield for light-induced H2 generation was measured for a previously optimized bio-hybrid cytochrome c 6-crosslinked PSI(C13G)-1,8-octanedithiol-[FeFe]-H2ase(C97G) (PSI-H2ase) nanoconstruct. The theoretical quantum yield for the PSI-H2ase nanoconstruct is 0.50 molecules of H2 per photon absorbed, which equates to a requirement of two photons per H2 generated. Illumination of the PSI-H2ase nanoconstruct with visible light between 400 and 700 nm resulted in an average quantum yield of 0.10-0.15 molecules of H2 per photon absorbed, which equates to a requirement of 6.7-10 photons per H2 generated. A possible reason for the difference between the theoretical and experimental quantum yield is the occurrence of non-productive PSI(C13G)-1,8-octanedithiol-PSIC13G (PSI-PSI) conjugates, which would absorb light without generating H2. Assuming the thiol-Fe coupling is equally efficient at producing PSI-PSI conjugates as well as in producing PSI-H2ase nanoconstructs, the theoretical quantum yield would decrease to 0.167 molecules of H2 per photon absorbed, which equates to 6 photons per H2 generated. This value is close to the range of measured values in the current study. A strategy that purifies the PSI-H2ase nanoconstructs from the unproductive PSI-PSI conjugates or that incorporates different chemistries on the PSI and [FeFe]-H2ase enzyme sites could potentially allow the PSI-H2ase nanoconstruct to approach the expected theoretical quantum yield for light-induced H2 generation.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Applegate AM,Lubner CE,Knörzer P,Happe T,Golbeck JHdoi
10.1007/s11120-014-0064-ysubject
Has Abstractpub_date
2016-01-01 00:00:00pages
5-11issue
1eissn
0166-8595issn
1573-5079pii
10.1007/s11120-014-0064-yjournal_volume
127pub_type
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更新日期:1993-01-01 00:00:00
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更新日期:1994-04-01 00:00:00
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更新日期:1988-10-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00032585
更新日期:1995-08-01 00:00:00
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更新日期:1980-06-01 00:00:00
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更新日期:1990-01-01 00:00:00
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doi:10.1007/BF00016274
更新日期:1993-05-01 00:00:00
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更新日期:2010-11-01 00:00:00
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更新日期:2013-11-01 00:00:00
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更新日期:1994-10-01 00:00:00
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更新日期:1996-06-01 00:00:00
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更新日期:2014-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2004-01-01 00:00:00
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doi:10.1007/BF00029737
更新日期:1986-01-01 00:00:00
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更新日期:1994-01-01 00:00:00
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journal_title:Photosynthesis research
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doi:10.1007/BF00035537
更新日期:1992-03-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-008-9312-3
更新日期:2008-08-01 00:00:00