Two conserved tryptophan residues are responsible for intrinsic fluorescence enhancement in Rubisco activase upon ATP binding.


:Two species-invariant tryptophan residues at positions 109 and 250 of tobacco Rubisco activase were identified by site-directed mutagenesis as being responsible for the increase in intrinsic fluorescence upon addition of ATP, which has been previously attributed to increased self-association. Substitution of W109, which is immediately prior to a 'P-loop' sequence in the ATP catalytic motif, with aromatic residues (Tyr or Phe), Cys or Lys eliminated both ATP hydrolysis and the intrinsic fluorescence enhancement. Although the W109 mutants bound ATP, ATP did not provide a partial protection against proteolysis by trypsin that was observed with the recombinant wild-type enzyme. In contrast, substitution of W250 with Tyr or Phe abolished about half (44%) of the increase in intrinsic fluorescence with ATP, but had little effect on ATP hydrolysis, ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activation or proteolytic protection with ATP. The substitution of the other tryptophan residues, W16 and W305, with phenylalanine did not significantly alter the change in intrinsic fluorescence upon addition of ATP. Therefore, W109 and W250 are the residues reporting the conformational change that increases the intrinsic fluorescence.


Photosynth Res


Photosynthesis research


Wang D,Portis AR Jr




Has Abstract


2006-05-01 00:00:00












  • Margareta Ryberg (1946-2012): a personal tribute.

    abstract::We pay tribute to the life and work of Margareta Ryberg (1946-2012). She was an expert on the different forms of protochlorophyll(ide), their protein partners, and their transformations in angiosperms; on the structural aspects, and the nature of prolamellar bodies, as well as on the localization of light-dependent NA...

    journal_title:Photosynthesis research

    pub_type: 传,历史文章,杂志文章


    authors: Ryberg H,Björn LO,Skagerfält B,Björn G

    更新日期:2012-09-01 00:00:00

  • Mechanisms of inorganic carbon acquisition in two estuarine Rhodophyceans: Bostrychia scorpioides (Hudson) ex Kützing Montagne and Catenella caespitosa (Withering) L. M. Irvine.

    abstract::Marine macroalgae possess a range of mechanisms to increase the availability of CO2 for fixation by ribulose-1,5-bisphosphate carboxylase/oxygenase. Of these, possession of a periplasmic or external carbonic anhydrase and the ability to use bicarbonate ions is widely distributed. The mechanisms of carbon acquisition w...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Ruiz-Nieto M,Fernández JA,Niell FX,Carmona R

    更新日期:2014-09-01 00:00:00

  • Supramolecular organization of the photosynthetic chain in chromatophores and cells of Rhodobacter sphaeroides.

    abstract::Flash-induced kinetics of the membrane potential increase related to electron transfer within the cytochrome (cyt) b/c1 complex (Phase III) and that of cyt c1+c2 reduction have been measured as a function of myxothiazol concentration in isolated chromatophores and whole cells of Rhodobacter sphaeroides. Upon addition ...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Joliot P,Verméglio A,Joliot A

    更新日期:1996-05-01 00:00:00

  • Chemical oxidation of the FMO antenna protein from Chlorobaculum tepidum.

    abstract::Effect of chemical oxidation by ferricyanide on bacteriochlorophyll a (BChl a) in the Fenna-Matthews-Olson protein (FMO) was studied using absorbance and fluorescence spectroscopy at ambient and cryogenic temperatures. Partially selective oxidation of pigments bound to the antenna complex was achieved and the probable...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Bina D,Blankenship RE

    更新日期:2013-09-01 00:00:00

  • High radiation and desiccation tolerance of nitrogen-fixing cultures of the cyanobacterium Anabaena sp. strain PCC 7120 emanates from genome/proteome repair capabilities.

    abstract::The filamentous nitrogen-fixing cyanobacterium, Anabaena sp. strain PCC 7120 was found to tolerate very high doses of 60Co-gamma radiation or prolonged desiccation. Post-stress, cells remained intact and revived all the vital functions. A remarkable capacity to repair highly disintegrated genome and recycle the damage...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Singh H,Anurag K,Apte SK

    更新日期:2013-10-12 00:00:00

  • The photosynthetic apparatus and photoinduced electron transfer in the aerobic phototrophic bacteria Roseicyclus mahoneyensis and Porphyrobacter meromictius.

    abstract::Photosynthetic electron transfer has been examined in whole cells, isolated membranes and in partially purified reaction centers (RCs) of Roseicyclus mahoneyensis, strain ML6 and Porphyrobacter meromictius, strain ML31, two species of obligate aerobic anoxygenic phototrophic bacteria. Photochemical activity in strain ...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Rathgeber C,Alric J,Hughes E,Verméglio A,Yurkov V

    更新日期:2012-02-01 00:00:00

  • Effects of polyploidy on photosynthesis.

    abstract::In polyploid plants the photosynthetic rate per cell is correlated with the amount of DNA per cell. The photosynthetic rate per unit leaf area is the product of the rate per cell times the number of photosynthetic cells per unit area. Therefore, the photosynthetic rate per unit leaf area will increase if there is a le...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Warner DA,Edwards GE

    更新日期:1993-02-01 00:00:00

  • Primary charge separation in Photosystem II.

    abstract::In this Minireview, we discuss a number of issues on the primary photosynthetic reactions of the green plant Photosystem II. We discuss the origin of the 683 and 679 nm absorption bands of the PS II RC complex and suggest that these forms may reflect the single-site spectrum with dominant contributions from the zero-p...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Dekker JP,Van Grondelle R

    更新日期:2000-01-01 00:00:00

  • Crystal structure of the Psb27 assembly factor at 1.6 Å: implications for binding to Photosystem II.

    abstract::The biogenesis and oxygen-evolving activity of cyanobacterial Photosystem II (PSII) is dependent on a number of accessory proteins not found in the crystallised dimeric complex. These include Psb27, a small lipoprotein attached to the lumenal side of PSII, which has been assigned a role in regulating the assembly of t...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Michoux F,Takasaka K,Boehm M,Komenda J,Nixon PJ,Murray JW

    更新日期:2012-02-01 00:00:00

  • Isolation and Characterization of an Outer Membrane Protein of Chlorobium tepidum.

    abstract::A protein was isolated from membranes of the green sulfur bacterium Chlorobium tepidum. This protein was characterized by gel electrophoresis, gel filtration, analytical ultracentrifugation and amino acid sequencing. The molecular weight of the purified protein was shown to be 26 kDa by SDS-PAGE. HPLC gelfiltration, S...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Aivaliotis M,Neofotistou E,Rémigy HW,Tsimpinos G,Lustig A,Lottspeich F,Tsiotis G

    更新日期:2004-02-01 00:00:00

  • Analogues of NADP(+) as inhibitors and coenzymes for NADP(+) malic enzyme from maize leaves.

    abstract::Structural analogues of the NADP(+) were studied as potential coenzymes and inhibitors for NADP(+) dependent malic enzyme from Zea mays L. leaves. Results showed that 1, N(6)-etheno-nicotinamide adenine dinucleotide phosphate (∈ NADP(+)), 3-acetylpyridine-adenine dinucleotide phosphate (APADP(+)), nicotinamide-hypoxan...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Spampinato CP,Paneth P,O'Leary MH,Andreo CS

    更新日期:1991-05-01 00:00:00

  • Probing the topography of the photosystem II oxygen evolving complex: PsbO is required for efficient calcium protection of the manganese cluster against dark-inhibition by an artificial reductant.

    abstract::The photosystem II (PSII) manganese-stabilizing protein (PsbO) is known to be the essential PSII extrinsic subunit for stabilization and retention of the Mn and Cl(-) cofactors in the oxygen evolving complex (OEC) of PSII, but its function relative to Ca(2+) is less clear. To obtain a better insight into the relations...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Popelkova H,Boswell N,Yocum C

    更新日期:2011-12-01 00:00:00

  • Accumulation of silver by Chromatium vinosum from solutions containing silver thiosulfate.

    abstract::The photosynthetic sulfur bacterium, Chromatium vinosum, was cultured in inorganic photographic processing solutions containing silver thiosulfate complex salt (AgNa3(S2O3)2) under light. It was found that Chromatium was resistant to Ag and accumulated granular silver in the membrane during growth. The amount of Ag ac...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Kitajima M

    更新日期:1986-01-01 00:00:00

  • Analysis of the proposed Fe-SX binding region of Photosystem 1 by site directed mutation of PsaA in Chlamydomonas reinhardtii.

    abstract::The psaA and psaB genes of the chloroplast genome in oxygenic photosynthetic organisms code for the major peptides of the Photosystem 1 reaction center. A heterodimer of the two polypeptides PsaA and PsaB is thought to bind the reaction center chlorophyll, P700, and the early electron acceptors A0, A1 and Fe-SX. Fe-SX...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Hallahan BJ,Purton S,Ivison A,Wright D,Evans MC

    更新日期:1995-11-01 00:00:00

  • Alternative photosystem I-driven electron transport routes: mechanisms and functions.

    abstract::In addition to the linear electron transport, several alternative Photosystem I-driven (PS I) electron pathways recycle the electrons to the intersystem electron carriers mediated by either ferredoxin:NADPH reductase, NAD(P)H dehydrogenase, or putative ferredoxin:plastoquinone reductase. The following functions have b...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Bukhov N,Carpentier R

    更新日期:2004-01-01 00:00:00

  • Thylakoid membrane landscape in the sixties: a tribute to Andrew Benson.

    abstract::Prior to the 1960s, the model for the molecular structure of cell membranes consisted of a lipid bilayer held in place by a thin film of electrostatically-associated protein stretched over the bilayer surface: (the Danielli-Davson-Robertson "unit membrane" model). Andrew Benson, an expert in the lipids of chloroplast ...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Anderson JM

    更新日期:2007-05-01 00:00:00

  • D1-protein dynamics in photosystem II: the lingering enigma.

    abstract::The D1/D2 heterodimer core is the heart of the photosystem II reaction center. A characteristic feature of this heterodimer is the differentially rapid, light-dependent degradation of the D1 protein. The D1 protein is possibly the most researched photosynthetic polypeptide, with aspects of structure-function, gene, me...

    journal_title:Photosynthesis research

    pub_type: 杂志文章,评审


    authors: Edelman M,Mattoo AK

    更新日期:2008-10-01 00:00:00

  • Dark-to-light transition in Synechococcus sp. PCC 7942 cells studied by fluorescence kinetics assesses plastoquinone redox poise in the dark and photosystem II fluorescence component and dynamics during state 2 to state 1 transition.

    abstract::We investigated the dark-to-light transition in Synechococcus sp. PCC 7942 cells by a detailed analysis of fluorescence transients induced by strong red light. The transients, recorded with high data-acquisition, revealed all the steps of the fast (OJIP; 10(-5)-1 s) and slow phase (PSM(T); 1-10(3) s), kinetically dist...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Tsimilli-Michael M,Stamatakis K,Papageorgiou GC

    更新日期:2009-03-01 00:00:00

  • Electron transfer in photosystem II.

    abstract::The picture presently emerging from studies on the mechanism of photosystem II electron transport is discussed. The reactions involved in excitation trapping, charge separation and stabilization of the charge pair in the reaction center, followed by the reactions with the substrates, plastoquinone reduction and water ...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Van Gorkom HJ

    更新日期:1985-01-01 00:00:00

  • Cyclic electron flow around Photosystem II in vivo.

    abstract::The oxygen flash yield (YO2) and photochemical yield of PS II (ΦPS II) were simultaneously detected in intact Chlorella cells on a bare platinum oxygen rate electrode. The two yields were measured as a function of background irradiance in the steady-state and following a transition from light to darkness. During stead...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Prasil O,Kolber Z,Berry JA,Falkowski PG

    更新日期:1996-06-01 00:00:00

  • Engineering cyanobacteria as photosynthetic feedstock factories.

    abstract::Carbohydrate feedstocks are at the root of bioindustrial production and are needed in greater quantities than ever due to increased prioritization of renewable fuels with reduced carbon footprints. Cyanobacteria possess a number of features that make them well suited as an alternative feedstock crop in comparison to t...

    journal_title:Photosynthesis research

    pub_type: 杂志文章,评审


    authors: Hays SG,Ducat DC

    更新日期:2015-03-01 00:00:00

  • Plasticity of photosynthetic performance of the Indian tree Butea monosperma TAUB at three sites with different microclimates.

    abstract::The Fabaceae tree Butea monosperma (TAUB.; syn. Erythrina monosperma (LAM.)) is widely distributed in Central and West-India. We studied it at three sites, i.e. at two locations with contrasting exposure (NE and SW, respectively) in a small mountain range with poor soil on highly drained rocky slopes and at a third lo...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Mikosch M,Kumari N,Sharma T,Sharma V,Gessler A,Fischer-Schliebs E,Lüttge U

    更新日期:2012-09-01 00:00:00

  • Reconstitution of energy transfer and electron transfer between solubilised pigment-protein complexes from thylakoid membranes. The role of acyl lipids.

    abstract::Solubilisation of thylakoid membranes from young leaves of Pisum sativum in the presence of Triton X-100 resulted in an almost complete loss of quenching of light-harvesting chlorophyll-protein (LHCP) fluorescence, as measured at 77°K. There were concomitant changes in the kinetics of light-saturation curves of electr...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Murphy DJ

    更新日期:1986-01-01 00:00:00

  • Thermal acclimation of photosynthesis: on the importance of adjusting our definitions and accounting for thermal acclimation of respiration.

    abstract::While interest in photosynthetic thermal acclimation has been stimulated by climate warming, comparing results across studies requires consistent terminology. We identify five types of photosynthetic adjustments in warming experiments: photosynthesis as measured at the high growth temperature, the growth temperature, ...

    journal_title:Photosynthesis research

    pub_type: 杂志文章,评审


    authors: Way DA,Yamori W

    更新日期:2014-02-01 00:00:00

  • NMR analysis of plant nitrogen metabolism.

    abstract::The analysis of primary and secondary nitrogen metabolism in plants by nuclear magnetic resonance (NMR) spectroscopy is comprehensively reviewed. NMR is a versatile analytical tool, and the combined use of (1)H, (2)H, (13)C, (14)N and (15)N NMR allows detailed investigation of the acquisition, assimilation and metabol...

    journal_title:Photosynthesis research

    pub_type: 杂志文章,评审


    authors: Mesnard F,Ratcliffe RG

    更新日期:2005-01-01 00:00:00

  • Chloroplasts in envelopes: CO(2) fixation by fully functional intact chloroplasts.

    abstract::Dan Arnon, Bob Whatley, Mary Belle Allen, and their colleagues, were the first to obtain evidence for 'complete photosynthesis by isolated chloroplasts' albeit at rates which were 1% or less of those displayed by the intact leaf. By the 1960s, partly in the hope of confirming full functionality, there was a perceived ...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Walker DA

    更新日期:2003-01-01 00:00:00

  • What role does sulpholipid play within the thylakoid membrane?

    abstract::Sulphoquinovosyldiacylglycerol is a negatively charged lipid which exists in the thylakoid membrane. It is proposed that a large proportion of this acidic lipid does not form a part of the bulk lipid matrix but is closely associated with protein complexes where it is tightly bound and participates in either optimising...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Barber J,Gounaris K

    更新日期:1986-01-01 00:00:00

  • Impact of esterified bacteriochlorophylls on the biogenesis of chlorosomes in Chloroflexus aurantiacus.

    abstract::A chlorosome is an antenna complex located on the cytoplasmic side of the inner membrane in green photosynthetic bacteria that contains tens of thousands of self-assembled bacteriochlorophylls (BChls). Green bacteria are known to incorporate various esterifying alcohols at the C-17 propionate position of BChls in the ...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Wang Y,Freund DM,Magdaong NM,Urban VS,Frank HA,Hegeman AD,Tang JK

    更新日期:2014-10-01 00:00:00

  • Properties of a pigment system consisting of carotenoids and reaction centre pigments in chromatophores of Rhodospirillum rubrum.

    abstract::A pigment system containing carotenoids and oxidised reaction centre pigments is present in chromatophores of Rhodospirillum rubrum and this pigment system may cause fluorescence quenching when a still unidentified chromatophore component is in its oxidised state. Besides by its action spectrum, this pigment system is...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Goedheer JC

    更新日期:1984-03-01 00:00:00

  • Photoprotection in intact cells of photosynthetic bacteria: quenching of bacteriochlorophyll fluorescence by carotenoid triplets.

    abstract::Upon high light excitation in photosynthetic bacteria, various triplet states of pigments can accumulate leading to harmful effects. Here, the generation and lifetime of flash-induced carotenoid triplets (3Car) have been studied by observation of the quenching of bacteriochlorophyll (BChl) fluorescence in different st...

    journal_title:Photosynthesis research

    pub_type: 杂志文章


    authors: Sipka G,Maróti P

    更新日期:2018-04-01 00:00:00