Abstract：
:Two species-invariant tryptophan residues at positions 109 and 250 of tobacco Rubisco activase were identified by site-directed mutagenesis as being responsible for the increase in intrinsic fluorescence upon addition of ATP, which has been previously attributed to increased self-association. Substitution of W109, which is immediately prior to a 'P-loop' sequence in the ATP catalytic motif, with aromatic residues (Tyr or Phe), Cys or Lys eliminated both ATP hydrolysis and the intrinsic fluorescence enhancement. Although the W109 mutants bound ATP, ATP did not provide a partial protection against proteolysis by trypsin that was observed with the recombinant wild-type enzyme. In contrast, substitution of W250 with Tyr or Phe abolished about half (44%) of the increase in intrinsic fluorescence with ATP, but had little effect on ATP hydrolysis, ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activation or proteolytic protection with ATP. The substitution of the other tryptophan residues, W16 and W305, with phenylalanine did not significantly alter the change in intrinsic fluorescence upon addition of ATP. Therefore, W109 and W250 are the residues reporting the conformational change that increases the intrinsic fluorescence.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Wang D,Portis AR Jrdoi
10.1007/s11120-006-9051-2subject
Has Abstractpub_date
2006-05-01 00:00:00pages
185-93issue
2eissn
0166-8595issn
1573-5079journal_volume
88pub_type
杂志文章abstract：:In many ways, the 1963 conference at Airlie House was an unusual event. The meeting convincingly demonstrated that photosynthetic research, previously reported mostly at conferences of botany and/or plant physiology, had become an independent and important field of biological research. New techniques had been develope...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/B:PRES.0000030427.35645.5c
更新日期：2004-01-01 00:00:00
abstract：:Temperature response of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) catalytic properties directly determines the CO2 assimilation capacity of photosynthetic organisms as well as their survival in environments with different thermal conditions. Despite unquestionable importance of Rubisco, the comprehensi...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-014-0067-8
更新日期：2015-02-01 00:00:00
abstract：:A combination of inhibitor and (15)N studies were used to investigate the photorespiratory nitrogen cycle in maize, a C4 plant. Inhibitors used included isonicotinyl hydrazide which blocks the conversion of glycine to serine, methionine sulfoximine an inhibitor of GS and azaserine an inhibitor of GOGAT. Results from l...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00041796
更新日期：1983-01-01 00:00:00
abstract：:A rapid procedure has been developed for the isolation of the photosystem two reaction centre complex (PS II RC) from a double mutant of Chlamydomonas reinhardtii, F54-14, which lacks the Photosystem one complex and the chloroplast ATPase. Thylakoid membranes are solubilised with 1.5% (w/v) Triton X-100 and the PS II ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00042974
更新日期：1995-02-01 00:00:00
abstract：:The previous work in our lab showed that the spinach chloroplast ATP synthase epsilon mutant with 3 amino acid residues deleted from the N-terminus had much lower ability to inhibit ATP hydrolysis and block proton leakage in comparison to a mutant with 1 or 2 residues deleted from the N-terminus. The present study aim...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-004-6554-6
更新日期：2005-01-01 00:00:00
abstract：:Historically, two main concepts guided research into possible mechanisms of light-induced atomic rearrangements in oxygenic photosynthesis: Photodecomposition of CO2 and photodecomposition of water. Both concepts envisioned photoinduced transfers of cumbersome whole atoms and not, as is currently held, photoinduced el...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00036216
更新日期：1991-09-01 00:00:00
abstract：:Bill Arnold's concept of photosynthesis as a solid state, as opposed to solution, phenomenon led him to an amazing series of fundamental discoveries. ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00040990
更新日期：1996-05-01 00:00:00
abstract：:A three-dimensional model of the core proteins D1 and D2, including the cofactors, that form the Photosystem II reaction centre of pea (Pisum sativum), has been generated. This model was built with a rule-based computer modelling system using the information from the crystal structures of the photosynthetic reaction c...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00033446
更新日期：1992-11-01 00:00:00
abstract：:Biogenesis of the photosynthetic apparatus in greening etiolated leaves of barley (Hordeum vulgare L) was investigated by an approach permitting investigation of this process under conditions that minimize differences in plastid development. Distributions of barley leaves greening for 24 h as to chlorophyll content an...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1016093127057
更新日期：2002-01-01 00:00:00
abstract：:Effect of chemical oxidation by ferricyanide on bacteriochlorophyll a (BChl a) in the Fenna-Matthews-Olson protein (FMO) was studied using absorbance and fluorescence spectroscopy at ambient and cryogenic temperatures. Partially selective oxidation of pigments bound to the antenna complex was achieved and the probable...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-013-9878-2
更新日期：2013-09-01 00:00:00
abstract：:Upon a sudden transition from high to low light, the rate of CO2 assimilation (AN) in some plants first decreases to a low level before gradually becoming stable. However, the underlying mechanisms remain controversial. The activity of chloroplast ATP synthase (gH+) is usually depressed under high light when compared ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-020-00725-y
更新日期：2020-04-01 00:00:00
abstract：:The effects of Fe resupply to Fe-deficient plants have been investigated in hydroponically-grown sugar beet. In the short-term (24 h) after Fe resupply, major changes were observed, although de novo chlorophyll (Chl) synthesis had not begun yet. Approximately 50% of the zeaxanthin was converted into violaxanthin, wher...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/B:PRES.0000011919.35309.5e
更新日期：2004-01-01 00:00:00
abstract：:Solubilisation of thylakoid membranes from young leaves of Pisum sativum in the presence of Triton X-100 resulted in an almost complete loss of quenching of light-harvesting chlorophyll-protein (LHCP) fluorescence, as measured at 77°K. There were concomitant changes in the kinetics of light-saturation curves of electr...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00037130
更新日期：1986-01-01 00:00:00
abstract：:When excited by ultraviolet radiation, leaves of a great number of species of higher plants exhibit emission of blue fluorescence, comparable in intensity to the red emission of chlorophyll. The fluorescence decay of the blue emission of spinach leaves recorded by single photon counting techniques is decomposed into e...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00033171
更新日期：1990-09-01 00:00:00
abstract：:A proton electrochemical potential across the membranes of photosynthetic purple bacteria is established by a light-driven proton pump mechanism: the absorbed light in the reaction center initiates electron transfer which is coupled to the vectorial displacement of protons from the cytoplasm to the periplasm. The stoi...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF02185435
更新日期：1993-07-01 00:00:00
abstract：:A study of the absorption and fluorescence characteristics of the D1/D2/cytb-559 reaction centre complex of Photosystem II has been carried out by gaussian decomposition of absorption spectra both at room temperature and 72 K and of the room temperature fluorescence spectrum. A five component fit was found in which th...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF02183048
更新日期：1994-09-01 00:00:00
abstract：:The effect of temperatures between 15 and 50°C on the light utilization efficiency of leaf net photosynthesis was studied in two C4 grasses and two C3 legumes. In the legumes, light utilization efficiency (μ mol of CO2 fixed per μ Einstein of incident photosynthetic quantum flux) declined linearly with temperature bet...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00034267
更新日期：1981-12-01 00:00:00
abstract：:Envelope membranes were isolated by sucrose density gradient floatation centrifugation from the homogenate of cyanelles prepared from Cyanophora paradoxa. Two yellow bands were separated after 40 h of centrifugation. The buoyant density of one of the two fractions (fraction Y2) coincided with that of inner envelope me...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-007-9156-2
更新日期：2007-07-01 00:00:00
abstract：:Maximum photosynthetic CO2 exchange rates (Pn) of single attached leaves were determined for several cassava cultivars selected from different habitats and grown in pots outdoors at CIAT, Colombia, S.A. Pn rates were in a narrow range of 22 to 26 μmol CO2 m(-2)s(-1) for all cultivars tested when measured at high photo...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00030025
更新日期：1984-09-01 00:00:00
abstract：:An immunological method for quantitative determination of photosynthetic fructose-1,6-bisphosphatase in crude extracts of leaves is proposed. It is based on the ELISA technique, and offers two modifications. A non-competitive technique has a higher sensitivity and is the right option for samples of low fructose-1,6-bi...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00032710
更新日期：1987-01-01 00:00:00
abstract：:The current study confirmed earlier conclusions regarding differential ozone (O(3)) tolerances of two soybean cultivars, Essex and Forrest, and evaluated antioxidant enzyme activities of these two varieties based on their performance under environmentally relevant, elevated O(3) conditions. The experiment was conducte...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1026500911237
更新日期：2000-01-01 00:00:00
abstract：:The unicellular green alga Chlamydomonas reinhardtii acclimates to low-CO2 (LC) conditions by actively transporting inorganic carbon (Ci) into the cell, resulting in an increase in photosynthetic efficiency. This mechanism is called the carbon-concentrating mechanism (CCM), and soluble protein LCIB is essential for th...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-013-9963-6
更新日期：2014-09-01 00:00:00
abstract：:In the leaf succulent Asclepiad Hoya carnosa (L.) R. Br., CAM photosynthesis occurred under well-watered conditions, as characterized by diurnal gas exchange and changes in titratable acidity. Following 10-12 days of severe water stress, the plants shifted from CAM to a modified CAM-idling mode of metabolism. CAM-idli...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00052124
更新日期：1983-09-01 00:00:00
abstract：:Earlier mass spectrometric measurements, in which oxygen evolution was measured following short saturating light flashes, indicated that with a time resolution of about 30 s no form of bound water and/or an oxidation product exists up to the redox state S3 of the oxygen evolving center (R. Radmer and O. Ollinger, 1986...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00046761
更新日期：1993-01-01 00:00:00
abstract：:Humic substances (HSs) can influence the growth and composition of freshwater phytoplankton assemblage. Since HSs contain many phenolic and quinonic moieties and cause growth reductions in eco-physiological field experiments, HSs are considered photosystem II herbicides. To test this specific mode of action in vivo an...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-018-0513-0
更新日期：2018-09-01 00:00:00
abstract：:The pigment composition of the light-harvesting complexes of Photosystem II (LHC II) has been determined for lettuce (Lactuca sativa). In common with other members of the composite, the photosynthetic tissues of this species may contain large amounts of the carotenoid lactucaxanthin (ε, ε-carotene-3,3'-diol) in additi...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00029940
更新日期：1995-03-01 00:00:00
abstract：:There is currently a renewed interest in developing microalgae as a source of renewable energy and fuel. Microalgae hold great potential as a source of biomass for the production of energy and fungible liquid transportation fuels. However, the technologies required for large-scale cultivation, processing, and conversi...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-011-9638-0
更新日期：2011-09-01 00:00:00
abstract：:This article provides a glimpse into the dawning of research on chlorophyll-protein complexes and a brief recollection of the path that led us to the identification of the photosystem II reaction center, i.e., the polypeptides that carry the site of primary charge separation in oxygenic photosynthesis. A preliminary v...
journal_title：Photosynthesis research
pub_type： 历史文章,杂志文章
doi：10.1007/s11120-008-9348-4
更新日期：2008-10-01 00:00:00
abstract：:When CO(2) supply is limited, aquatic photosynthetic organisms induce a CO(2)-concentrating mechanism (CCM) and acclimate to the CO(2)-limiting environment. Although the CCM is well studied in unicellular green algae such as Chlamydomonas reinhardtii, physiological aspects of the CCM and its associated genes in multic...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-010-9614-0
更新日期：2011-09-01 00:00:00
abstract：:Heliobacteria contain Type I reaction centers (RCs) and a homodimeric core, but unlike green sulfur bacteria, they do not contain an extended antenna system. Given their simplicity, the heliobacterial RC (HbRC) should be ideal for the study of a prototypical homodimeric RC. However, there exist enormous gaps in our kn...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-007-9162-4
更新日期：2007-04-01 00:00:00
