Phycobilisome integrity and functionality in lipid unsaturation and xanthophyll mutants in Synechocystis.

Abstract:

:The major light-harvesting system in cyanobacteria, the phycobilisome, is an essential component of the photosynthetic apparatus that regulates the utilization of the natural light source-the Sun. Earlier works revealed that the thylakoid membrane composition and its physical properties might have an important role in antennas docking. Polyunsaturated lipids and xanthophylls are among the most significant modulators of the physical properties of thylakoid membranes. In the nature, the action of these molecules is orchestrated in response to environmental stimuli among which the growth temperature is the most influential. In order to further clarify the significance of thylakoid membrane physical properties for the phycobilisomes assembly (i.e. structural integrity) and their ability to efficiently direct the excitation energy towards the photosynthetic complexes, in this work, we utilize cyanobacterial Synechocystis sp. PCC 6803 mutants deficient in polyunsaturated lipids (AD mutant) and xanthophylls (RO mutant), as well as a strain depleted of both xanthophylls and polyunsaturated lipids (ROAD multiple mutant). For the first time, we discuss the effect of those mutations on the phycobilisomes assembly, integrity and functionality at optimal (30 °C) and moderate low (25 °C) and high (35 °C) temperatures. Our results show that xanthophyll depletion exerts a much stronger effect on both phycobilisome's integrity and the response of cells to growth at suboptimal temperatures than lipid unsaturation level. The strongest effects were observed for the combined ROAD mutant, which exhibited thermally destabilized phycobilisomes and a population of energetically uncoupled phycocyanin units.

journal_name

Photosynth Res

journal_title

Photosynthesis research

authors

Vajravel S,Laczkó-Dobos H,Petrova N,Herman É,Kovács T,Zakar T,Todinova S,Taneva S,Kovács L,Gombos Z,Tóth T,Krumova S

doi

10.1007/s11120-020-00776-1

subject

Has Abstract

pub_date

2020-08-01 00:00:00

pages

179-188

issue

2

eissn

0166-8595

issn

1573-5079

pii

10.1007/s11120-020-00776-1

journal_volume

145

pub_type

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