Abstract：
:Ten strains from a collection of mutants ofSynechocystis 6803 defective in Photosystem II (PS II) function were transformed with chromosomal DNA of wild-type and mutant cells. Cross hybridization data allowed to identify four groups of PS II-mutants. Highly efficient transformation was observed between different mutant groups, but not within the groups. Restoration of photosynthetic activity of the mutant cells was also achieved by transformation with different parts of a 5.6 kbBam HI fragment of wild typeSynechocystis DNA containing thepsbB gene. Each group of mutants was transformed to photoautotrophic growth by specific subfragments of thepsbB gene. DNA fragments of four selected mutant strains hybridizing with thepsbB gene were isolated and sequenced. The mutations were identified as a single nucleotide insertion or substitution leading to stop codon formation in two of the mutants, as a deletion of 12 nucleotides, or as a nucleotide substitution resulting in an amino acid substitution in the other two mutants. Deletion of 12 nucleotides in mutant strain PMB1 and stop codon formation in strain NF16 affect membrane-spanning regions of the gene product, the CP 47 protein.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Ermakova SY,Elanskaya IV,Kallies KU,Weihe A,Börner T,Shestakov SVdoi
10.1007/BF02187472subject
Has Abstractpub_date
1993-08-01 00:00:00pages
139-46issue
2eissn
0166-8595issn
1573-5079journal_volume
37pub_type
杂志文章abstract：:Chlorophyll (Chl) b serves an essential function in accumulation of light-harvesting complexes (LHCs) in plants. In this article, this role of Chl b is explored by considering the properties of Chls and the ligands with which they interact in the complexes. The overall properties of the Chls, not only their spectral f...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-007-9181-1
更新日期：2007-11-01 00:00:00
abstract：:Photosynthesis and carbohydrate metabolism of higher plants need to be tightly regulated to prevent tissue damage during environmental changes. The intracellular position of chloroplasts changes due to a changing light regime. Chloroplast avoidance and accumulation response under high and low light, respectively, are ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-020-00795-y
更新日期：2021-01-01 00:00:00
abstract：:Photosystem II (PSII) catalyzes the oxidation of water to O2 at the manganese-containing, oxygen-evolving complex (OEC). Photoexcitation of PSII results in the oxidation of the OEC; four sequential oxidation reactions are required for the generation and release of molecular oxygen. Therefore, with flash illumination, ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-007-9147-3
更新日期：2007-06-01 00:00:00
abstract：:Photosynthesis was well-established on the earth at least 3.5 thousand million years ago, and it is widely believed that these ancient organisms had similar metabolic capabilities to modern cyanobacteria. This requires that development of two photosystems and the oxygen evolution capability occurred very early in the...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：
更新日期：1992-01-01 00:00:00
abstract：:A correlated influence of cation concentration and excitation energy level on PS II Abbreviations: AMPD = 2-amino-2-methyl-1,3-propanediol; DCIP = 2-6 dichlorophenol indophenol; DCIPH2 = DCIP in reduced state; DCIPr = rate of DCIP-reduction; DCMU = 3-(3,4 dichlorophenyl)1-1 dimethyl-urea; Fv = variable fluorescence; F...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00018228
更新日期：1980-06-01 00:00:00
abstract：:Here, we describe a new imaging setup able to assess in vivo photosynthetic activity. The system specifically measures time-resolved chlorophyll fluorescence in response to light. It is composed of a fast digital camera equipped with a wide-angle lens for the analysis of samples up to 10 x 10 cm, i.e. entire plants or...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-009-9487-2
更新日期：2009-10-01 00:00:00
abstract：:Illumination of the dark-incubated cells of Synechocystis PCC6803 caused recovery of both respiratory activity of oxygen uptake and PS I-cyclic electron flow, which was monitored by the dark reduction of P700(+) in the presence of DCMU after a 50 ms pulse light (MT) under background far-red light, but the effects were...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1017946524199
更新日期：2001-01-01 00:00:00
abstract：:As part of an ongoing investigation into the effects of long-term UV-B radiation exposure on the growth and morphology of woody perennials, the gas exchange and photosynthesis of five common deciduous tree species were measured. All five tree species had been exposed to UV-B radiation for 5 years, in the field, at an ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1010620228989
更新日期：2001-01-01 00:00:00
abstract：:Photosynthetic acclimation varies among species, which likely reveals variations at the biochemical level in the pathways that constitute carbon assimilation and energy transfer. Local adaptation and phenotypic plasticity affect the environmental response of photosynthesis. Phenotypic plasticity allows for a wide arra...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-013-9816-3
更新日期：2014-02-01 00:00:00
abstract：:Chloroplasts of a chlorophyll (Chl) b-less barley mutant were solubilized with digitonin and fractionated by polyacrylamide gel electrophoresis with sodium deoxycholate in the running buffer. By this procedure, in contrast to using sodium dodecylsulfate (SDS) for solubilization, a Chl a-protein analogous to the major ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00018272
更新日期：1987-01-01 00:00:00
abstract：:This review describes the progress in our understanding of the structure of the Mn complex in Photosystem II over the last two decades. Emphasis is on the research from our laboratory, especially the results from X-ray absorption spectroscopy, low temperature electron paramagnetic resonance and electron spin echo enve...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00046751
更新日期：1993-01-01 00:00:00
abstract：:Sulphoquinovosyldiacylglycerol is a negatively charged lipid which exists in the thylakoid membrane. It is proposed that a large proportion of this acidic lipid does not form a part of the bulk lipid matrix but is closely associated with protein complexes where it is tightly bound and participates in either optimising...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00029747
更新日期：1986-01-01 00:00:00
abstract：:We investigated the dark-to-light transition in Synechococcus sp. PCC 7942 cells by a detailed analysis of fluorescence transients induced by strong red light. The transients, recorded with high data-acquisition, revealed all the steps of the fast (OJIP; 10(-5)-1 s) and slow phase (PSM(T); 1-10(3) s), kinetically dist...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-009-9405-7
更新日期：2009-03-01 00:00:00
abstract：:This contribution gives a brief survey of the short history since the discovery of the aerobic phototrophic bacteria to focus on a general evolutionary scenario. Most of the citations are of reviews that have covered the earlier literature and to which the reader is directed at appropriate places in the following text...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1020493518379
更新日期：2002-01-01 00:00:00
abstract：:The photoenzymatic cycle of the light-dependent NADPH:protochlorophyllide oxidoreductase (LPOR) was investigated in situ during early stages of development of bean leaves under light-dark cycles (LDC). In the experimental system used in this study, prolamellar bodies developed during night periods and disappeared duri...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-007-9274-x
更新日期：2008-04-01 00:00:00
abstract：:I provide here a personal perspective on (i) the awards that were given to young investigators at the 2008 and 2009 Gordon Research Conferences on Biochemistry/Biophysics of Photosynthesis; and (ii) the ambiance at these conferences through some photographs, particularly of the 2009 conference. ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-009-9479-2
更新日期：2009-10-01 00:00:00
abstract：:The major light-harvesting system in cyanobacteria, the phycobilisome, is an essential component of the photosynthetic apparatus that regulates the utilization of the natural light source-the Sun. Earlier works revealed that the thylakoid membrane composition and its physical properties might have an important role in...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-020-00776-1
更新日期：2020-08-01 00:00:00
abstract：:The potential role of foliar carbon export features in the acclimation of photosynthetic capacity to differences and changes in light environment was evaluated. These features included apoplastic vs. symplastic phloem loading, density of loading veins, plasmodesmatal frequency in intermediary cells, and the ratio of l...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-006-9123-3
更新日期：2007-11-01 00:00:00
abstract：:The electron transport rates of photosystems II and I, amounts of electron carriers, coupling factor activity and photosynthetic rates were investigated in thylakoids isolated from pea plants grown under a wide range of light intensities (16 h light-8 h dark). The electron transport rates of PS II and PS I, as partial...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00028525
更新日期：1984-06-01 00:00:00
abstract：:Due to various activities associated with nuclear industry, uranium is migrated to aquatic environments like groundwater, ponds or oceans. Uranium forms stable carbonate complexes in the oxic waters of pH 7-10 which results in a high degree of uranium mobility. Microorganisms employ various mechanisms which significan...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-013-9928-9
更新日期：2013-11-01 00:00:00
abstract：:A chloroplast preparation was extracted from squash (Cucurbita pepo (L.) var. Senator). Enrichment of intact chloroplasts was achieved by continuous free-flow electrophoresis. The addition of monoterpenes, detergent and free fatty acids changed the elecrophoretic separation pattern characteristically. Monoterpene-depe...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00054124
更新日期：1991-06-01 00:00:00
abstract：:Soybean [Glycine max (L.) Merr. cv. Williams 82 and A3127] plants were grown in the field under long-term soil moisture deficit and irrigation to determine the effects of severe drought stress on the photosynthetic capacity of soybean leaves. Afternoon leaf water potentials, stomatal conductances, intercellular CO2 co...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00051735
更新日期：1990-07-01 00:00:00
abstract：:Historically, two main concepts guided research into possible mechanisms of light-induced atomic rearrangements in oxygenic photosynthesis: Photodecomposition of CO2 and photodecomposition of water. Both concepts envisioned photoinduced transfers of cumbersome whole atoms and not, as is currently held, photoinduced el...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00036216
更新日期：1991-09-01 00:00:00
abstract：:Pigment-protein-complexes of two chlorophyll b deficient mutants of Arabidopsis and from the wild type were separated electrophoretically. Light-harvesting proteins were absent in the chlorophyll b free mutant ch(1) and their amount was reduced in the mutant ch(2) which has a reduced content of chlorophyll b. The rati...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00041827
更新日期：1983-01-01 00:00:00
abstract：:The excitation energy transfer between carotenoid and chlorophyll (Chl) in the cytochrome b ( 6 ) f complex from Bryopsis corticulans (B. corticulans), in which the carotenoid is 9-cis-alpha-carotene, was investigated by means of fluorescence excitation and sub-microsecond time-resolved absorption spectroscopies. The ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-005-9020-1
更新日期：2006-04-01 00:00:00
abstract：:The light-harvesting complex (LHC) of higher plants isolated using Triton X-100 has been studied during its transformation into a monomeric form known as CPII. The change was accomplished by gradually increasing the concentration of the detergent, sodium dodecyl sulfate (SDS). Changes in the red spectral region of the...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00029727
更新日期：1986-01-01 00:00:00
abstract：:Maximum photosynthetic CO2 exchange rates (Pn) of single attached leaves were determined for several cassava cultivars selected from different habitats and grown in pots outdoors at CIAT, Colombia, S.A. Pn rates were in a narrow range of 22 to 26 μmol CO2 m(-2)s(-1) for all cultivars tested when measured at high photo...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00030025
更新日期：1984-09-01 00:00:00
abstract：:It had been proposed that a loop, typically containing 26 or 27 amino acids, which is only present in monomeric, ferredoxin-dependent, "plant-type" glutamate synthases and is absent from the catalytic α-subunits of both NADPH-dependent, heterodimeric glutamate synthases found in non-photosynthetic bacteria and NADH-de...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-014-0044-2
更新日期：2015-02-01 00:00:00
abstract：:Minimal photosynthetic catalytic F1(αβ) core complexes, containing equimolar ratios of the α and β subunits, were isolated from membrane-bound spinach chloroplast CF1 and Rhodospirillum rubrum chromatophore RrF1. A CF1-α3β3 hexamer and RrF1-α1β1 dimer, which were purified from the respective F1(αβ) complexes, exhibit ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00020418
更新日期：1995-11-01 00:00:00
abstract：:Heliobacteria are a recently discovered group of anoxygenic phototrophic bacteria, first described in 1983. Heliobacteria contain bacteriochlorophyll g, a pigment unique to species of this group, and synthesize the simplest photosynthetic complexes of all known phototrophs. Also, unlike all other phototrophs, heliobac...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-009-9516-1
更新日期：2010-06-01 00:00:00
