Abstract:
:To determine the fluorescence properties of cyanobacterial Photosystem I (PS I) in relatively intact systems, fluorescence emission from 20 to 295 K and polarization at 77 K have been measured from phycobilisomes-less thylakoids of Synechocystis sp. PCC 6803 and a mutant strain lacking Photosystem II (PS II). At 295 K, the fluorescence maxima are 686 nm in the wild type from PS I and PS II and at 688 nm from PS I in the mutant. This emission is characteristic of bulk antenna chlorophylls (Chls). The 690-nm fluorescence component of PS I is temperature independent. For wild-type and mutant, 725-nm fluorescence increases by a factor of at least 40 from 295 to 20 K. We model this temperature dependence assuming a small number of Chls within PS I, emitting at 725 nm, with an energy level below that of the reaction center, P700. Their excitation transfer rate to P700 decreases with decreasing temperature increasing the yield of 725-nm fluorescence.Fluorescence excitation spectra of polarized emission from low-energy Chls were measured at 77 and 295 K on the mutant lacking PS II. At excitation wavelengths longer than 715 nm, 760-nm emission is highly polarized indicating either direct excitation of the emitting Chls with no participation in excitation transfer or total alignment of the chromophores. Fluorescence at 760 nm is unpolarized for excitation wavelengths shorter than 690 nm, inferring excitation transfer between Chls before 760-nm fluorescence occurs.Our measurements illustrate that: 1) a single group of low-energy Chls (F725) of the core-like PS I complex in cyanobacteria shows a strongly temperature-dependent fluorescence and, when directly excited, nearly complete fluorescence polarization, 2) these properties are not the result of detergent-induced artifacts as we are examining intact PS I within the thylakoid membrane of S. 6803, and 3) the activation energy for excitation transfer from F725 Chls to P700 is less than that of F735 Chls in green plants; F725 Chls may act as a sink to locate excitations near P700 in PS I.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Wittmershaus BP,Woolf VM,Vermaas WFdoi
10.1007/BF00028785subject
Has Abstractpub_date
1992-02-01 00:00:00pages
75-87issue
2eissn
0166-8595issn
1573-5079journal_volume
31pub_type
杂志文章abstract::This review presents a summary of recent investigations examining the structure and function of the chlorophyll-proteins CPa-1 (CP47) and CPa-2 (CP43). Comparisons of the derived amino acid sequences of these proteins suggest sites for chlorophyll binding and for interactions between these chlorophyll-proteins and oth...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00032639
更新日期:1990-04-01 00:00:00
abstract::The effect of acetate metabolism on the light energy distribution between the two photosystems, on the PS II/PS I stoichiometry and on the expression of psbA and psbB and psaA genes was investigated in the green alga, Chlamydobotrys stellata during autotrophic (CO(2)), mixotrophic (CO(2) plus acetate) and photoheterot...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1010650532693
更新日期:2000-01-01 00:00:00
abstract::Oxygenic photosynthesis requires chlorophyll (Chl) for the absorption of light energy, and charge separation in the reaction center of photosystem I and II, to feed electrons into the photosynthetic electron transfer chain. Chl is bound to different Chl-binding proteins assembled in the core complexes of the two photo...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-015-0154-5
更新日期:2015-12-01 00:00:00
abstract::The nucleotide sequences of the genes coding for the subunits of the Photosystem I (PS I) core, PsaA and PsaB were determined for the marine prokaryotic oxyphototrophs Prochlorococcus sp. MED4 (CCMP1378), P. marinus SS120 (CCMP1375) and Synechococcus sp. WH7803. Divergence of these sequences from those of both freshwa...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1006445810996
更新日期:2000-01-01 00:00:00
abstract::Pigment-protein-complexes of two chlorophyll b deficient mutants of Arabidopsis and from the wild type were separated electrophoretically. Light-harvesting proteins were absent in the chlorophyll b free mutant ch(1) and their amount was reduced in the mutant ch(2) which has a reduced content of chlorophyll b. The rati...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00041827
更新日期:1983-01-01 00:00:00
abstract::Mike Wasielewski's pioneering work on Photosystem II photochemistry has an important place in the history of photosynthesis; we are proud to have been associated with him in making those first measurements. Here, we present our association and publications with him, and provide some of the history behind this research...
journal_title:Photosynthesis research
pub_type: 传,历史文章,杂志文章
doi:10.1007/s11120-009-9505-4
更新日期:2010-01-01 00:00:00
abstract::The content and composition of pigments and acyl lipids (monogalactosyl diacylglycerol, digalactosyl diacylglycerol and phosphatidyl glycerol) have been investigated in developing chloroplasts isolated from successive 2-cm sections along the leaves of wheat seedlings grown either under 100, 30 or 3 W·m(-2). In all exa...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00035244
更新日期:1986-01-01 00:00:00
abstract::The localization of isoprenoid lipids in chloroplasts, the accumulation of particular isoprenoids under high irradiance conditions, and channelling of photosynthetically fixed carbon into plastidic thylakoid isoprenoids, volatile isoprenoids, and cytosolic sterols are reviewed. During leaf and chloroplast development ...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-007-9204-y
更新日期:2007-05-01 00:00:00
abstract::This article gives an historical overview of our group's research on various metabolite translocators of chloroplasts, such as the translocators for phosphorylated intermediates of the Calvin-Benson cycle and of glycolysis, of ADP and ATP, of dicarboxylates, of pyruvate and of hexoses; how it began and where it led to...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1020475803784
更新日期:2002-01-01 00:00:00
abstract::Recent magnetic-resonance work on YŻ suggests that this species exhibits considerable motional flexibility in its functional site and that its phenol oxygen is not involved in a well-ordered hydrogen-bond interaction (Tang et al., submitted; Tommos et al., in press). Both of these observations are inconsistent with a ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00020428
更新日期:1995-11-01 00:00:00
abstract::The effect of light on the metabolism of ammonia was studied by subjecting detached maize leaves to 150 or 1350 μmol m(-2) s(-1) PAR during incubation with the leaf base in 2 mM (15)NH4Cl. After up to 60 min, leaves were extracted. Ammonia, glutamine, glycine, serine, alanine, and aspartate were separated by isotherma...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00049278
更新日期:1985-09-01 00:00:00
abstract::Almost 1 year has passed since the death of Wolfgang Haehnel on 13.5.2013, after a long fight against cancer. With his death, the photosynthesis community has lost an excellent scientist, a critical peer, and a stimulating sparring partner in discussions: I also lost a good friend. ...
journal_title:Photosynthesis research
pub_type: 传,历史文章
doi:10.1007/s11120-014-9999-2
更新日期:2014-06-01 00:00:00
abstract::There is currently a renewed interest in developing microalgae as a source of renewable energy and fuel. Microalgae hold great potential as a source of biomass for the production of energy and fungible liquid transportation fuels. However, the technologies required for large-scale cultivation, processing, and conversi...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-011-9638-0
更新日期:2011-09-01 00:00:00
abstract::Several models have been proposed to interpret the mechanism of proton-pumping associated with the electron transfer reactions in the cytochrome b/f complex. Energetics considerations suggest that the proton pump is coupled to the oxidation of cytochrome b by plastoquinone. Experiments performed in living cells under ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00029737
更新日期:1986-01-01 00:00:00
abstract::Isolated mesophyll cells from darkened leaves of the C(4) plant Digitaria sanguinalis keep functional plasmodesmata that allow the free exchange of low molecular mass compounds with the surrounding medium. This cell suspension system has been used to measure C(4) PEPC activity in situ using a spectrophotometric assay....
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/B:PRES.0000017179.31351.f0
更新日期:2004-01-01 00:00:00
abstract::Phosphoenolpyruvate carboxylase from leaves of the C4 plant Setaria verticillata (L.) Beauv. is activated by light; day levels of activity are reached after 30 minutes of illumination. Photoactivation is prevented by inhibitors of photosynthetic electron flow or of photophosphorylation and by D,L-glyceraldehyde, which...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00028842
更新日期:1988-06-01 00:00:00
abstract::The Fabaceae tree Butea monosperma (TAUB.; syn. Erythrina monosperma (LAM.)) is widely distributed in Central and West-India. We studied it at three sites, i.e. at two locations with contrasting exposure (NE and SW, respectively) in a small mountain range with poor soil on highly drained rocky slopes and at a third lo...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-012-9770-5
更新日期:2012-09-01 00:00:00
abstract::We investigated several photosynthetic parameters of a virescent mutant of durum wheat and of its wild-type. Electron transport rate to ferricyanide was the same in the two genotypes when expressed on leaf area basis while O2 evolution of the leaf tissue in saturating light and CO2 was slightly higher in the yellow ge...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00033365
更新日期:1989-08-01 00:00:00
abstract::The light-harvesting complex (LHC) of higher plants isolated using Triton X-100 has been studied during its transformation into a monomeric form known as CPII. The change was accomplished by gradually increasing the concentration of the detergent, sodium dodecyl sulfate (SDS). Changes in the red spectral region of the...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00029727
更新日期:1986-01-01 00:00:00
abstract::The first research paper on photosynthesis in China was published by T.T. Li(2) in 1929. Two photosynthesis laboratories were established in Shanghai and Beijing in the 1950s and the 1960s, respectively. A photophosphorylation 'intermediate' was discovered after the energy conversion process was separated into light a...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1024946222187
更新日期:2003-01-01 00:00:00
abstract::In polyploid plants the photosynthetic rate per cell is correlated with the amount of DNA per cell. The photosynthetic rate per unit leaf area is the product of the rate per cell times the number of photosynthetic cells per unit area. Therefore, the photosynthetic rate per unit leaf area will increase if there is a le...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00014744
更新日期:1993-02-01 00:00:00
abstract::Plant terpenoids are among the most diverse group of naturally-occurring organic compounds known, and several are used in contemporary consumer products. Terpene synthase enzymes catalyze complex rearrangements of carbon skeleton precursors to yield thousands of unique chemical structures that range in size from the s...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-014-9979-6
更新日期:2015-03-01 00:00:00
abstract::We generated transgenic tobacco plants with high levels of fructose-1,6-bisphosphatase expressing cyanobacterialfructose-1,6-/sedoheptulose-1,7-bisphosphatase in the cytosol. At ambient CO(2) levels (360 ppm), growth, photosynthetic activity, and fresh weight were unchanged but the sucrose/hexose/starch ratio was slig...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-011-9645-1
更新日期:2011-05-01 00:00:00
abstract::The current status of research on the structure, regulation, mechanism and importance of Rubisco activase is reviewed. The activase is now recognized to be a member of the AAA(+) family, whose members participate in macromolecular complexes that perform diverse chaperone-like functions. The conserved nucleotide-bindin...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1022458108678
更新日期:2003-01-01 00:00:00
abstract::A hypothesis is proposed to explain the function of Cl(-) in activating the oxygenevolving complex (OEC) of photosystem II (PS II), based on the results of recent (35)Cl-NMR studies. The putative mechanism involves Cl(-) binding to two types of sites. An intrinsic site is suggested to be composed of three histidyl res...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00029400
更新日期:1987-09-01 00:00:00
abstract::Chlorophyll f is a photosynthetic pigment that was discovered in 2010. In this study, we present investigations on spectral and dynamic characteristics of singlet-excited and triplet states of Chl f with the application of ultrafast time-resolved absorption and fluorescence spectroscopies. The pigment was studied at r...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-014-9981-z
更新日期:2014-07-01 00:00:00
abstract::Macroalgae occur in the marine benthos from the upper intertidal to depths of more than 200 m, contributing up to 1 Pg C per year to global primary productivity. Freshwater macroalgae are mainly green (Chlorophyta) with some red (Rhodophyta) and a small contribution of brown (Phaeophyceae) algae, while in the ocean al...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-012-9768-z
更新日期:2012-09-01 00:00:00
abstract::The role of the ferredoxin:thioredoxin system in the reversible light activation of chloroplast enzymes by thiol-disulfide interchange with thioredoxins is now well established. Recent fruitful collaboration between biochemists and structural biologists, reflected by the shared authorship of the paper, allowed to solv...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/B:PRES.0000017194.34167.6d
更新日期:2004-01-01 00:00:00
abstract::The origin of the extraordinary high redox potential of P680, the primary electron donor of Photosystem II, is still unknown. Photochemically induced dynamic nuclear polarisation (photo-CIDNP) 13C magic-angle spinning (MAS) NMR is a powerful method to study primary electron donors. In order to reveal the electronic st...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-005-0411-0
更新日期:2005-06-01 00:00:00
abstract::Historically, two main concepts guided research into possible mechanisms of light-induced atomic rearrangements in oxygenic photosynthesis: Photodecomposition of CO2 and photodecomposition of water. Both concepts envisioned photoinduced transfers of cumbersome whole atoms and not, as is currently held, photoinduced el...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00036216
更新日期:1991-09-01 00:00:00