Preparation and functional characterization of thylakoids from Arabidopsis thaliana.

Abstract:

:A protocol for the isolation of functional thylakoids from Arabidopsis thaliana leaves was developed. The critical factor in obtaining active, coupled and stable preparation is the inclusion of EDTA and EGTA in the grinding buffer. Preparations were characterized with respect to the whole or partial electron transport chain, ATP/NADPH, ATP/O(2) and PS II/chlorophyll ratios. Sensitivity to a light-chill photoinhibitory treatment was also determined by evaluating the decrease in both maximal photochemical efficiency (Fv/Fm) and in electron transport rate.

journal_name

Photosynth Res

journal_title

Photosynthesis research

authors

Casazza AP,Tarantino D,Soave C

doi

10.1023/A:1011818021875

subject

Has Abstract

pub_date

2001-01-01 00:00:00

pages

175-80

issue

2

eissn

0166-8595

issn

1573-5079

journal_volume

68

pub_type

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