X-ray spectroscopy of the Mn4Ca cluster in the water-oxidation complex of Photosystem II.

Abstract:

:The water-oxidation complex of Photosystem II (PS II) contains a heteronuclear cluster of 4 Mn atoms and a Ca atom. Ligands to the metal cluster involve bridging O atoms, and O and N atoms from amino acid side-chains of the D1 polypeptide of PS II, with likely additional contributions from water and CP43. Although moderate resolution X-ray diffraction-based structures of PS II have been reported recently, and the location of the Mn4Ca cluster has been identified, the structures are not resolved at the atomic level. X-ray absorption (XAS), emission (XES), resonant inelastic X-ray scattering (RIXS) and extended X-ray absorption fine structure (EXAFS) provide independent and potentially highly accurate sources of structural and oxidation-state information. When combined with polarized X-ray studies of oriented membranes or single-crystals of PS II, a more detailed picture of the cluster and its disposition in PS II is obtained.

journal_name

Photosynth Res

journal_title

Photosynthesis research

authors

Sauer K,Yano J,Yachandra VK

doi

10.1007/s11120-005-0638-9

subject

Has Abstract

pub_date

2005-01-01 00:00:00

pages

73-86

issue

1

eissn

0166-8595

issn

1573-5079

journal_volume

85

pub_type

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