Abstract:
:Solubilisation of thylakoid membranes from young leaves of Pisum sativum in the presence of Triton X-100 resulted in an almost complete loss of quenching of light-harvesting chlorophyll-protein (LHCP) fluorescence, as measured at 77°K. There were concomitant changes in the kinetics of light-saturation curves of electron transport from 2,6-dichlorophenolindophenol/ascorbate to methyl viologen. These effects were accompenied by a physical dissociation of LHCP polypeptides from photosystem I (PSI) and photosystem II (PSII) polypeptides, as determined by polyacrylamide gel-electrophoresis. Detergent-dialysis in the presence of exogenous purified galactolipids, about 80% of which were linoleoyl molecular species, only partially reversed these effects. However, detergent-dialysis using the phospholipids, phosphatidylglycerol and phosphatidylcholine, resulted in the substantial restoration of 77°K fluorescence quenching and the restoration of both emission spectra and electron transport kinetics of both Photosystems I and II that were typical of native membranes.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Murphy DJdoi
10.1007/BF00037130subject
Has Abstractpub_date
1986-01-01 00:00:00pages
219-33issue
3eissn
0166-8595issn
1573-5079journal_volume
8pub_type
杂志文章abstract::Due to various activities associated with nuclear industry, uranium is migrated to aquatic environments like groundwater, ponds or oceans. Uranium forms stable carbonate complexes in the oxic waters of pH 7-10 which results in a high degree of uranium mobility. Microorganisms employ various mechanisms which significan...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-013-9928-9
更新日期:2013-11-01 00:00:00
abstract::A synthetic fluorochromous lipid, rhodaminyl triglyceride (rhodaminyl TG), was intercalated into isolated thylakoid membranes of chloroplasts up to 30 molecules per 100 molecules of chlorophyll. As a result of fluorochrome presence, an absorption band appeared in a yellow-green spectrum region, its intensity being com...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00047079
更新日期:1990-11-01 00:00:00
abstract::The effects of Fe resupply to Fe-deficient plants have been investigated in hydroponically-grown sugar beet. In the short-term (24 h) after Fe resupply, major changes were observed, although de novo chlorophyll (Chl) synthesis had not begun yet. Approximately 50% of the zeaxanthin was converted into violaxanthin, wher...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/B:PRES.0000011919.35309.5e
更新日期:2004-01-01 00:00:00
abstract::The origin of the extraordinary high redox potential of P680, the primary electron donor of Photosystem II, is still unknown. Photochemically induced dynamic nuclear polarisation (photo-CIDNP) 13C magic-angle spinning (MAS) NMR is a powerful method to study primary electron donors. In order to reveal the electronic st...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-005-0411-0
更新日期:2005-06-01 00:00:00
abstract::Joan Mary Anderson, known to most people as Jan, was born on May 12, 1932 in Dunedin, New Zealand. She died on August 28, 2015 in Canberra, Australia. To celebrate her life, we present here a brief biography, some comments on her discoveries in photosynthesis during a career spanning more than half a century, and remi...
journal_title:Photosynthesis research
pub_type: 传,历史文章,杂志文章
doi:10.1007/s11120-016-0287-1
更新日期:2016-08-01 00:00:00
abstract::The absorption spectra of two light harvesting complexes from higher plants, CP29 and LHC II, have been analysed in the Soret region in order to obtain a description in terms of the absorption spectra of the individual pigments. This information is of great practical use when applying spectroscopic techniques to the s...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1006455230379
更新日期:2000-01-01 00:00:00
abstract::DCMU (3-(3,4-dichlorophenyl)-1,1-dimethylurea) at concentrations higher than 10 μM suppresses the second time range delayed fluorescence (DF) of pea chloroplasts, due to inhibition of the oxidizing side of photosystem II (PS II). The inhibition of the reducing side of PS II resulting in the suppression of millisecond ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00054986
更新日期:1988-01-01 00:00:00
abstract::Photosystem I particles containing 30-40 chlorophyll a molecules per primary electron donor P700 were subjected to 1.5 ps low density laser flashes at 610 nm resulting in excitation of the antenna chlorophyll a molecules followed by energy transfer to P700 and subsequent oxidation of P700. Absorbance changes were moni...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00047947
更新日期:1987-01-01 00:00:00
abstract::Lipids are important components of transmembrane protein complexes. In order to study the roles of lipids in photosystem II (PSII), we treated the PSII core dimer complex from a thermophilic cyanobacterium Thermosynechococcus vulcanus with phospholipase A(2) (PLA(2)) and lipase, and examined their effects on PSII stru...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-008-9335-9
更新日期:2008-10-01 00:00:00
abstract::Energy storage measurements by modulated photothermal radiometry (PTR) were carried out on intact leaves to assess the value of the PTR method for photosynthesis research. In particular, correlations to the redox state of P700 under various conditions were examined. PTR monitors modulated light conversion to heat by s...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00035379
更新日期:1991-08-01 00:00:00
abstract::Time-resolved (TR) infrared (IR) spectroscopy in the nanosecond to second timescale has been extensively used, in the last 30 years, in the study of photosynthetic systems. Interesting results have also been obtained at lower time resolution (minutes or even hours). In this review, we first describe the used technique...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-016-0305-3
更新日期:2017-02-01 00:00:00
abstract::Ten strains from a collection of mutants ofSynechocystis 6803 defective in Photosystem II (PS II) function were transformed with chromosomal DNA of wild-type and mutant cells. Cross hybridization data allowed to identify four groups of PS II-mutants. Highly efficient transformation was observed between different mutan...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF02187472
更新日期:1993-08-01 00:00:00
abstract::Photosystem II (PSII) catalyzes the oxidation of water to O2 at the manganese-containing, oxygen-evolving complex (OEC). Photoexcitation of PSII results in the oxidation of the OEC; four sequential oxidation reactions are required for the generation and release of molecular oxygen. Therefore, with flash illumination, ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-007-9147-3
更新日期:2007-06-01 00:00:00
abstract::A study of the absorption and fluorescence characteristics of the D1/D2/cytb-559 reaction centre complex of Photosystem II has been carried out by gaussian decomposition of absorption spectra both at room temperature and 72 K and of the room temperature fluorescence spectrum. A five component fit was found in which th...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF02183048
更新日期:1994-09-01 00:00:00
abstract::A guided tour through much of photosynthesis research as I saw it, 1936-2001, is presented here. For earlier perspectives, see Myers 1974 (Plant Physiol 54: 420-426) and 1996 (Photosynth Res 50: 195-208). ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1020475502933
更新日期:2002-01-01 00:00:00
abstract::Water vapor was added differentially to the gas streams entering the cells of three makes of differential infrared carbon dioxide analysers. Analyser deflections were compared with those expected from dilution of the carbon dioxide by the additional gas. Tests were made at 0, 365, and 730 cm(3) m(-3) concentrations of...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00054104
更新日期:1985-12-01 00:00:00
abstract::The binding characteristics and the inhibitory power of atrazine and DCMU towards uncoupled electron flow activity were studied in acyl lipid-depleted thylakoid membranes from atrazine-susceptible and-resistant biotypes of Solanum nigrum L. For this purpose, phospholipase A2 from Vipera russelli and the lipase from Rh...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00049537
更新日期:1992-01-01 00:00:00
abstract::Phosphatidylglycerol (PG), containing the unique fatty acid Delta3, trans-16:1-hexadecenoic acid, is a minor but ubiquitous lipid component of thylakoid membranes of chloroplasts and cyanobacteria. We investigated its role in electron transfers and structural organization of Photosystem II (PSII) by treating Arabidops...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-006-9126-0
更新日期:2007-07-01 00:00:00
abstract::While interest in photosynthetic thermal acclimation has been stimulated by climate warming, comparing results across studies requires consistent terminology. We identify five types of photosynthetic adjustments in warming experiments: photosynthesis as measured at the high growth temperature, the growth temperature, ...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-013-9873-7
更新日期:2014-02-01 00:00:00
abstract::The analysis of primary and secondary nitrogen metabolism in plants by nuclear magnetic resonance (NMR) spectroscopy is comprehensively reviewed. NMR is a versatile analytical tool, and the combined use of (1)H, (2)H, (13)C, (14)N and (15)N NMR allows detailed investigation of the acquisition, assimilation and metabol...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-004-2081-8
更新日期:2005-01-01 00:00:00
abstract::The cyanobacterial small CAB-like proteins (SCPs) are one-helix proteins with compelling similarity to the first and third transmembrane helix of proteins belonging to the CAB family of light-harvesting complex proteins in plants. The SCP proteins are transiently expressed at high light intensity and other stress cond...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-007-9244-3
更新日期:2008-02-01 00:00:00
abstract::Glycolate oxidase (GO; EC 1.1.3.1) was purified from the leaves of three plant species:Amaranthus hypochondriacus L.(NAD-ME type C4 dicot),Pisum sativum L. (C3 species) andParthenium hysterophorus L. (C3-C4. intermediate). A flavin moiety was present in the enzyme from all the three species. The enzyme from the C4 pla...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF02184284
更新日期:1996-03-01 00:00:00
abstract::This concise review describes the current status of research into how carotenoids function in bacterial photosynthesis. This is illustrated by reference to very recent studies on both the photoprotective and antenna functions of carotenoids. The major remaining open questions on the detailed molecular mechanisms invol...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1014715114520
更新日期:2001-01-01 00:00:00
abstract::The availability of a complete genome database for the cyanobacterium Synechocystis sp. PCC6803 (glucose-tolerant strain) has raised expectations that this organism would become a reference strain for work aimed at understanding the CO(2)-concentrating mechanism (CCM) in cyanobacteria. However, the amount of physiolog...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1025850230977
更新日期:2003-01-01 00:00:00
abstract::The seasonal maximum in photosynthetic CO2 exchange rate (CER) and the cessation of leaf expansion in soybeans (Glycine max (L.) Merr) accompany fruiting under normal agricultural conditions. To investigate whether these phenomena were obligatively tied together, we caused early flowering of long-season varieties by i...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00034108
更新日期:1982-12-01 00:00:00
abstract::Mike Wasielewski's pioneering work on Photosystem II photochemistry has an important place in the history of photosynthesis; we are proud to have been associated with him in making those first measurements. Here, we present our association and publications with him, and provide some of the history behind this research...
journal_title:Photosynthesis research
pub_type: 传,历史文章,杂志文章
doi:10.1007/s11120-009-9505-4
更新日期:2010-01-01 00:00:00
abstract::Bill Arnold's concept of photosynthesis as a solid state, as opposed to solution, phenomenon led him to an amazing series of fundamental discoveries. ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00040990
更新日期:1996-05-01 00:00:00
abstract::The picture presently emerging from studies on the mechanism of photosystem II electron transport is discussed. The reactions involved in excitation trapping, charge separation and stabilization of the charge pair in the reaction center, followed by the reactions with the substrates, plastoquinone reduction and water ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00032785
更新日期:1985-01-01 00:00:00
abstract::The role of photosynthetic pigments in the development of separation methods in biochemistry during the period 1900-1980 is described beginning with M. Tswett who introduced separation of chlorophylls and carotenoids on columns and coined the term chromatography in 1906. In Uppsala, T. Svedberg developed the ultracent...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1024944606930
更新日期:2003-01-01 00:00:00
abstract::Maximum photosynthetic CO2 exchange rates (Pn) of single attached leaves were determined for several cassava cultivars selected from different habitats and grown in pots outdoors at CIAT, Colombia, S.A. Pn rates were in a narrow range of 22 to 26 μmol CO2 m(-2)s(-1) for all cultivars tested when measured at high photo...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00030025
更新日期:1984-09-01 00:00:00