Abstract:
:A new binding site for anions which inhibit the water oxidizing complex (WOC) of Photosystem II in spinach has been identified. Anions which bind to this site inhibit the flash-induced S2/S0 catalase reaction (2H2O2→2H2O+O2) of the WOC by displacing hydrogen peroxide. Using a mass spectrometer and gas permeable membrane to detect the (32)O2 product, the yield and lifetime of the active state of the flash-induced catalase (to be referred to simply as 'flash-catalase') reaction were measured after forming the S2 or S0-states by a short flash. The increase in flash-catalase activity with H2O2 concentration exhibits a Km=10-20 mM, and originates from an increase in the lifetime by 20-fold of the active state. The increased lifetime in the presence of peroxide is ascribed to formation of the long-lived S0-state at the expense of the unstable S2-state. The anion inhibition site differs from the chloride site involved in stimulating the photolytic water oxidation reaction (2H2O→O2+4e(-)+4H(+)). Whereas water oxidation requires Cl(-) and is inhibited with increasing effectiveness by F(-)≪CN(-)≪N3 (-), the flash-catalase reaction is weakly inhibited by Cl(-), and with increasing effectiveness by F(-)≪CN(-), N3 (-). Unlike water oxidation, chloride is unable to suppress or reverse inhibition of the flash-catalase reaction caused by these anions. The inhibitor effectiveness correlates with the pKa of the conjugate acid, suggesting that the protonated species may be the active inhibitor. The reduced activity arises from a shortening of the lifetime of the flash-induced catalase active state by 3-10 fold owing to stronger anion binding in the flash-induced states, S2 and S0, than in the dark S-states, S1 and S-1. To account for the paradoxical result that higher anion concentrations are required to inhibit at lower H2O2 concentrations, where S2 forms initially after the flash, than at higher H2O2 concentrations, where S0 forms initially after the flash, stronger anion binding to the S0-state than to the S2-state is proposed. A kinetic model is given which accounts for these equilibria with anions and H2O2. The rate constant for the formation/release of O2 by reduction of S2 in the WOC is <0.4 s(-1).
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Mano J,Kawamoto K,Dismukes GC,Asada Kdoi
10.1007/BF00046771subject
Has Abstractpub_date
1993-01-01 00:00:00pages
433-40issue
3eissn
0166-8595issn
1573-5079journal_volume
38pub_type
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00030061
更新日期:1990-01-01 00:00:00
abstract::In vitro studies of the carotenoid peridinin, which is the primary pigment from the peridinin chlorophyll-a protein (PCP) light harvesting complex, showed a strong dependence on the lifetime of the peridinin lowest singlet excited state on solvent polarity. This dependence was attributed to the presence of an intramol...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-005-1447-x
更新日期:2005-11-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-008-9315-0
更新日期:2008-08-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/B:PRES.0000036881.23512.42
更新日期:2004-01-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/B:PRES.0000030428.36950.43
更新日期:2004-01-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-010-9614-0
更新日期:2011-09-01 00:00:00
abstract::Solubilisation of thylakoid membranes from young leaves of Pisum sativum in the presence of Triton X-100 resulted in an almost complete loss of quenching of light-harvesting chlorophyll-protein (LHCP) fluorescence, as measured at 77°K. There were concomitant changes in the kinetics of light-saturation curves of electr...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00037130
更新日期:1986-01-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-006-9051-2
更新日期:2006-05-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1010695402963
更新日期:2000-01-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00032236
更新日期:1995-07-01 00:00:00
abstract::The thylakoid membrane protein PsbS is critical for quenching excessive excitation energy in mechanisms that involve the light-harvesting complexes of photosystem II. Liposomes of thylakoid lipids have been shown to be a very good platform to study photosynthetic membrane proteins and their interactions. In this study...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-015-0176-z
更新日期:2016-01-01 00:00:00
abstract::Most chloroplasts undergo changes in composition, function and structure in response to growth irradiance. However, Tradescantia albiflora, a facultative shade plant, is unable to modulate its light-harvesting components and has the same Chl a/Chl b ratios and number of functional PS II and PS I reaction centres on a ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1010681721929
更新日期:2001-01-01 00:00:00
abstract::Chlorophyll f is a photosynthetic pigment that was discovered in 2010. In this study, we present investigations on spectral and dynamic characteristics of singlet-excited and triplet states of Chl f with the application of ultrafast time-resolved absorption and fluorescence spectroscopies. The pigment was studied at r...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-014-9981-z
更新日期:2014-07-01 00:00:00
abstract::Dan Arnon, Bob Whatley, Mary Belle Allen, and their colleagues, were the first to obtain evidence for 'complete photosynthesis by isolated chloroplasts' albeit at rates which were 1% or less of those displayed by the intact leaf. By the 1960s, partly in the hope of confirming full functionality, there was a perceived ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1024962328483
更新日期:2003-01-01 00:00:00
abstract::In plants, chlorophylls (chlorophyll a and chlorophyll b) are the most abundant tetrapyrrole molecules and are essential for photosynthesis. The first committed step of chlorophyll biosynthesis is the insertion of Mg(2+) into protoporphyrin IX, and thus subsequent steps of the biosynthesis are called the Mg branch. As...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-008-9291-4
更新日期:2008-05-01 00:00:00
abstract::We have previously investigated the response mechanisms of photosystem II complexes from spinach to strong UV and visible irradiations (Wei et al J Photochem Photobiol B 104:118-125, 2011). In this work, we extend our study to the effects of strong light on the unusual cyanobacterium Acaryochloris marina, which is abl...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-013-9946-7
更新日期:2013-11-01 00:00:00
abstract::The factors governing quinone protein binding in the photosynthetic bacterium Rps. viridis are investigated via use of empirical intermolecular energy calculations using the GRID method (PJ Goodford (1985) J Med Chem 28: 849-854). A probe atom corresponding to a carbonyl oxygen atom is used to investigate regions exhi...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00027142
更新日期:1994-01-01 00:00:00
abstract::Recycling of carbon by the photorespiratory pathway involves enzymatic steps in the chloroplast, mitochondria, and peroxisomes. Most of these reactions are essential for plants growing under ambient CO(2) concentrations. However, some disruptions of photorespiratory metabolism cause subtle phenotypes in plants grown i...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-011-9651-3
更新日期:2011-09-01 00:00:00
abstract::Far-red light photoacclimation (FaRLiP) is a mechanism that allows some cyanobacteria to utilize far-red light (FRL) for oxygenic photosynthesis. During FaRLiP, cyanobacteria remodel photosystem (PS) I, PS II, and phycobilisomes while synthesizing Chl d, Chl f, and far-red-absorbing phycobiliproteins, and these change...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-016-0309-z
更新日期:2017-02-01 00:00:00
abstract::The cyanobacterial small CAB-like proteins (SCPs) are one-helix proteins with compelling similarity to the first and third transmembrane helix of proteins belonging to the CAB family of light-harvesting complex proteins in plants. The SCP proteins are transiently expressed at high light intensity and other stress cond...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-007-9244-3
更新日期:2008-02-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/B:PRES.0000017194.34167.6d
更新日期:2004-01-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00032919
更新日期:1985-01-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00055064
更新日期:1987-01-01 00:00:00
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journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00046879
更新日期:1988-10-01 00:00:00
abstract::The effect of light on the metabolism of ammonia was studied by subjecting detached maize leaves to 150 or 1350 μmol m(-2) s(-1) PAR during incubation with the leaf base in 2 mM (15)NH4Cl. After up to 60 min, leaves were extracted. Ammonia, glutamine, glycine, serine, alanine, and aspartate were separated by isotherma...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00049278
更新日期:1985-09-01 00:00:00
abstract::Phycobilisomes, comprised of both chromophoric (phycobiliproteins) and non-chromophoric (linker polypeptides) proteins, are light-harvesting complexes present in the prokaryotic cyanobacteria and the eukaryotic red algae. Many cyanobacteria exhibit complementary chromatic adaptation, a process which enables these orga...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00047680
更新日期:1988-07-01 00:00:00
abstract::A time line of important research relating to anoxygenic photosynthetic organisms is presented. The time line includes discoveries of organisms, metabolic capabilities, molecular complexes and genetic systems. It also pinpoints important milestones in our understanding of the structure, function, organization, assembl...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/B:PRES.0000030448.24695.ec
更新日期:2004-01-01 00:00:00
abstract::This minireview summarizes the current state of knowledge concerning the role of Cl(-) in the oxygen-evolving complex (OEC) of photosystem II (PSII). The model that proposes that Cl(-) is a Mn ligand is discussed in light of more recent work. Studies of Cl(-) specificity, stoichiometry, kinetics, and retention by extr...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-006-9121-5
更新日期:2007-07-01 00:00:00
abstract::The genome of the purple non-sulphur photosynthetic bacterium Rhodopseudomonas acidophila has been found to contain multiple copies of puc light-harvesting (LH2) peripheral antenna complex genes. Three wild-type isolates each exhibiting dissimilar peripheral antenna complex phenotypes in response to growth at reduced ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00034783
更新日期:1996-09-01 00:00:00
abstract::Earlier mass spectrometric measurements, in which oxygen evolution was measured following short saturating light flashes, indicated that with a time resolution of about 30 s no form of bound water and/or an oxidation product exists up to the redox state S3 of the oxygen evolving center (R. Radmer and O. Ollinger, 1986...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00046761
更新日期:1993-01-01 00:00:00