Abstract:
:A non-competitive enzyme-linked immunosorbent assay (ELISA) which enables detection of as little as 0.1 ng cytochrome f in leaf extracts has been developed. No evidence for specific or non-specific interference by proteins other than cytochrome f was found. The assay was applied to a comparative study of age-related changes in the cytochrome f content of leaves of Festuca pratensis Huds. cv. Rossa, and a non-yellowing mutant genotype (Bf993) having a lesion in the mechanism responsible for thylakoid membrane disassembly. Cytochrome f in senescent leaves of the latter genotype was found to be present at significantly higher levels than in the wild-type, implying an inability on the part of the mutant to degrade this protein. The results obtained by ELISA were confirmed by antibody probing of Western blots.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Davies TG,Thomas H,Rogers LJdoi
10.1007/BF00032649subject
Has Abstractpub_date
1990-04-01 00:00:00pages
99-108issue
1eissn
0166-8595issn
1573-5079journal_volume
24pub_type
杂志文章abstract::Oxygenic photosynthesis requires chlorophyll (Chl) for the absorption of light energy, and charge separation in the reaction center of photosystem I and II, to feed electrons into the photosynthetic electron transfer chain. Chl is bound to different Chl-binding proteins assembled in the core complexes of the two photo...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-015-0154-5
更新日期:2015-12-01 00:00:00
abstract::When excited by ultraviolet radiation, leaves of a great number of species of higher plants exhibit emission of blue fluorescence, comparable in intensity to the red emission of chlorophyll. The fluorescence decay of the blue emission of spinach leaves recorded by single photon counting techniques is decomposed into e...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00033171
更新日期:1990-09-01 00:00:00
abstract::Unique light-harvesting antennas in the green sulfur bacterium Chlorobaculum tepidum, called chlorosomes, consist of self-aggregates of bacteriochlorophyll (BChl) c. In the biosynthesis of BChl c, BciC demethoxycarbonylase removes the C132-methoxycarbonyl group to facilitate the self-aggregation of BChl c. We previous...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-018-0573-1
更新日期:2019-03-01 00:00:00
abstract::We investigated the dark-to-light transition in Synechococcus sp. PCC 7942 cells by a detailed analysis of fluorescence transients induced by strong red light. The transients, recorded with high data-acquisition, revealed all the steps of the fast (OJIP; 10(-5)-1 s) and slow phase (PSM(T); 1-10(3) s), kinetically dist...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-009-9405-7
更新日期:2009-03-01 00:00:00
abstract::Maximum photosynthetic CO2 exchange rates (Pn) of single attached leaves were determined for several cassava cultivars selected from different habitats and grown in pots outdoors at CIAT, Colombia, S.A. Pn rates were in a narrow range of 22 to 26 μmol CO2 m(-2)s(-1) for all cultivars tested when measured at high photo...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00030025
更新日期:1984-09-01 00:00:00
abstract::The excitation energy transfer between carotenoid and chlorophyll (Chl) in the cytochrome b ( 6 ) f complex from Bryopsis corticulans (B. corticulans), in which the carotenoid is 9-cis-alpha-carotene, was investigated by means of fluorescence excitation and sub-microsecond time-resolved absorption spectroscopies. The ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-005-9020-1
更新日期:2006-04-01 00:00:00
abstract::A fluorescence video imaging system utilizing relatively inexpensive commercial components is described. The instrument utilizes a black and white CCD video camera detector, a commercial video imaging board and a IBM-AT compatible computer. The color output of the imaging board greatly aids in the users ability to vis...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00048977
更新日期:1990-10-01 00:00:00
abstract::Recently, a number of techniques, some of them relatively new and many often used in combination, have given a clearer picture of the dynamic role of electron transport in Photosystem I of photosynthesis and of coupled cyclic photophosphorylation. For example, the photoacoustic technique has detected cyclic electron t...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00033035
更新日期:1993-06-01 00:00:00
abstract::Little is known about the mechanistic basis for the movement of promiscuous nucleic acids across cell membranes. To address this problem we sought conditions that would permit the entry of plasmid DNA into isolated, intact pea chloroplasts. DNA uptake did not occur normally, but was induced by hypotonic treatments, by...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00020448
更新日期:1995-11-01 00:00:00
abstract::We have constructed a mutant Synechococcus sp. PCC 7942, termed R2HECAT, in which the entire phycobilisome rod operon has been deleted. In the whole cell absorption spectra of R2HECAT, the peak corresponding to phycocyanin (PC), λmax≈620 nm, could not be detected. However, a single pigment-protein fraction with λmax=6...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00032236
更新日期:1995-07-01 00:00:00
abstract::This work addresses the question of occurrence and function of photosystem II (PSII) in bundle sheath (BS) cells of leaves possessing NADP-malic enzyme-type C4 photosynthesis (Zea mays). Although no requirement for PSII activity in the BS has been established, several component proteins of PSII have been detected in B...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-014-0009-5
更新日期:2014-10-01 00:00:00
abstract::Three Chl-protein complexes were isolated from thylakoid membranes of Bryopsis maxima and Ulva pertusa, marine green algae that inhabit the intertidal zone of the Pacific Ocean off the eastern coast of Japan by dodecyl-beta-D-maltoside polyacrylamide gel electrophoresis. The slowest-moving fractions showed low Chl a/b...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-006-9064-x
更新日期:2006-07-01 00:00:00
abstract::A chloroplast preparation was extracted from squash (Cucurbita pepo (L.) var. Senator). Enrichment of intact chloroplasts was achieved by continuous free-flow electrophoresis. The addition of monoterpenes, detergent and free fatty acids changed the elecrophoretic separation pattern characteristically. Monoterpene-depe...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00054124
更新日期:1991-06-01 00:00:00
abstract::A chlorosome is an antenna complex located on the cytoplasmic side of the inner membrane in green photosynthetic bacteria that contains tens of thousands of self-assembled bacteriochlorophylls (BChls). Green bacteria are known to incorporate various esterifying alcohols at the C-17 propionate position of BChls in the ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-014-0017-5
更新日期:2014-10-01 00:00:00
abstract::Fluorescence and electron paramagnetic resonance measurements were used to study selenium influence on photosystem activity in rape seedlings affected by Cd stress. Water cultures containing Hoagland nutrients were supplemented with 400 microM of CdCl(2), 2 microM of Na(2)SeO(4) and a mixture of both CdCl(2) and Na(2)...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-010-9551-y
更新日期:2010-07-01 00:00:00
abstract::We generated transgenic tobacco plants with high levels of fructose-1,6-bisphosphatase expressing cyanobacterialfructose-1,6-/sedoheptulose-1,7-bisphosphatase in the cytosol. At ambient CO(2) levels (360 ppm), growth, photosynthetic activity, and fresh weight were unchanged but the sucrose/hexose/starch ratio was slig...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-011-9645-1
更新日期:2011-05-01 00:00:00
abstract::The 16th International Congress on Photosynthesis will be held August 11-16, 2013 in St. Louis, Missouri, USA. The congress will include 15 plenary lectures, 21 scientific symposia, poster sessions, exhibitors, opening reception, final banquet, excursions and accompanying persons program. The congress is organized as ...
journal_title:Photosynthesis research
pub_type:
doi:10.1007/s11120-013-9847-9
更新日期:2013-07-01 00:00:00
abstract::The xanthophyll cycle represents one of the important photoprotection mechanisms in plant cells. In the present review, we summarize current knowledge about the violaxanthin cycle of vascular plants, green and brown algae, and the diadinoxanthin cycle of the algal classes Bacillariophyceae, Xanthophyceae, Haptophyceae...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-010-9536-x
更新日期:2010-11-01 00:00:00
abstract::Chlorophyll (Chl) is an essential component of the photosynthetic apparatus. Embedded into Chl-binding proteins, Chl molecules play a central role in light harvesting and charge separation within the photosystems. It is critical for the photosynthetic cell to not only ensure the synthesis of a sufficient amount of new...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-013-9797-2
更新日期:2014-02-01 00:00:00
abstract::Photosynthesis was well-established on the earth at least 3.5 thousand million years ago, and it is widely believed that these ancient organisms had similar metabolic capabilities to modern cyanobacteria. This requires that development of two photosystems and the oxygen evolution capability occurred very early in the...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:
更新日期:1992-01-01 00:00:00
abstract::The three major chlorophyll-proteins of spinach chloroplasts were solubilized with digitonin and isolated by electrophoresis with deoxycholate. The gel bands were identified from their absorption and fluorescence spectra measured at 77 K. The slowest moving band was a Photosystem I complex (CPI); the second, a Photosy...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00054145
更新日期:1983-12-01 00:00:00
abstract::The analysis of primary and secondary nitrogen metabolism in plants by nuclear magnetic resonance (NMR) spectroscopy is comprehensively reviewed. NMR is a versatile analytical tool, and the combined use of (1)H, (2)H, (13)C, (14)N and (15)N NMR allows detailed investigation of the acquisition, assimilation and metabol...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-004-2081-8
更新日期:2005-01-01 00:00:00
abstract::Peridinin-chlorophyll-protein (PCP), containing differently absorbing chlorophyll derivatives, are good models with which to study energy transfer among monomeric chlorophylls (Chls) by both bulk and single-molecule spectroscopy. They can be obtained by reconstituting the N-terminal domain of the protein (N-PCP) with ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-007-9277-7
更新日期:2008-02-01 00:00:00
abstract::The photophysical and photochemical reactions, after light absorption by a photosynthetic pigment-protein complex, are among the fastest events in biology, taking place on timescales ranging from tens of femtoseconds to a few nanoseconds. The advent of ultrafast laser systems that produce pulses with femtosecond durat...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-009-9454-y
更新日期:2009-08-01 00:00:00
abstract::Chlorosomes, the light-harvesting apparatus of green bacteria, are a unique antenna system, in which pigments are organized in aggregates rather than associated with proteins. Isolated chlorosomes from the green sulphur bacterium Chlorobium tepidum contain 10 surface-exposed proteins. Treatment of chlorosomes from Chl...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-005-3757-4
更新日期:2005-11-01 00:00:00
abstract::The first research paper on photosynthesis in China was published by T.T. Li(2) in 1929. Two photosynthesis laboratories were established in Shanghai and Beijing in the 1950s and the 1960s, respectively. A photophosphorylation 'intermediate' was discovered after the energy conversion process was separated into light a...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1024946222187
更新日期:2003-01-01 00:00:00
abstract::Several protein-coding genes from land plant chloroplasts have been shown to contain introns. The majority of these introns resemble the fungal mitochondrial group II introns due to considerable nucleotide sequence homology at their 5' and 3' ends and they can readily be folded to form six hairpins characteristic of t...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00039484
更新日期:1988-04-01 00:00:00
abstract::This paper is concerned with relating thermoluminescence to the total free-energy change, ΣG, involved in detrapping a particular electron-hole pair as a photosynthetic sample is warmed from an initial low temperature. It extends a mathematical discussion of four possible mechanisms introduced in an earlier paper [DeV...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00032597
更新日期:1990-05-01 00:00:00
abstract::Since 1986, non-uniform photosynthesis over the leaf area that may be attributed to patchy stomatal closure, has been an important issue in stress physiology of photosynthesis. In this review, I first outline the gaseous environment within the intercellular spaces, because this is the most fundamental background of th...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00035537
更新日期:1992-03-01 00:00:00
abstract::It has been known for some time that bicarbonate reverses the inhibition, by formate under HCO3 (-)-depletion conditions, of electron transport in thylakoid membranes. It has been shown that the major effect is on the electron acceptor side of photosystem II, at the site of plastoquinone reduction. After presenting a ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00114571
更新日期:1988-01-01 00:00:00