Immunochemical quantification of cytochrome f in leaves of a non-yellowing senescence mutant of Festuca pratensis.

Abstract:

:A non-competitive enzyme-linked immunosorbent assay (ELISA) which enables detection of as little as 0.1 ng cytochrome f in leaf extracts has been developed. No evidence for specific or non-specific interference by proteins other than cytochrome f was found. The assay was applied to a comparative study of age-related changes in the cytochrome f content of leaves of Festuca pratensis Huds. cv. Rossa, and a non-yellowing mutant genotype (Bf993) having a lesion in the mechanism responsible for thylakoid membrane disassembly. Cytochrome f in senescent leaves of the latter genotype was found to be present at significantly higher levels than in the wild-type, implying an inability on the part of the mutant to degrade this protein. The results obtained by ELISA were confirmed by antibody probing of Western blots.

journal_name

Photosynth Res

journal_title

Photosynthesis research

authors

Davies TG,Thomas H,Rogers LJ

doi

10.1007/BF00032649

subject

Has Abstract

pub_date

1990-04-01 00:00:00

pages

99-108

issue

1

eissn

0166-8595

issn

1573-5079

journal_volume

24

pub_type

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