Abstract：
:In this communication, we report our progress on the development of low-frequency Fourier transform infrared (FTIR) spectroscopic techniques to study metal-substrate and metal-ligand vibrational modes in the Photosystem II/oxygen-evolving complex (PS II/OEC). This information will provide important structural and mechanistic insight into the OEC. Strong water absorption in the low-frequency region (below 1000 cm(-1)), a lack of suitable materials, and temperature control problems have limited previous FTIR spectroscopic studies of the OEC to higher frequencies (>1000 cm(-1)). We have overcome these technical difficulties that have blocked access to the low-frequency region and have developed successive instruments that allow us to move deeper into the low-frequency region (down to 350 cm(-1)), while increasing both data accumulation efficiency and S/N ratio. We have detected several low-frequency modes in the S(2)/S(1)spectrum that are specifically associated with these two states. Our results demonstrate the utility of FTIR techniques in accessing low-frequency modes in Photosystem II and in proteins generally.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Chu HA,Gardner MT,Hillier W,Babcock GTdoi
10.1023/A:1010779327960subject
Has Abstractpub_date
2000-01-01 00:00:00pages
57-63issue
1-2eissn
0166-8595issn
1573-5079journal_volume
66pub_type
杂志文章abstract：:A short list of the twentieth century's dominant figures in photosynthesis would unquestionably include Otto Warburg. One of his many discoveries, the 'bicarbonate effect' remains a lasting puzzle to his heirs in the field. Recent developments in this area of research have renewed interest and call for a re-examinatio...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1020447030191
更新日期：2002-01-01 00:00:00
abstract：:Photosynthetic acclimation varies among species, which likely reveals variations at the biochemical level in the pathways that constitute carbon assimilation and energy transfer. Local adaptation and phenotypic plasticity affect the environmental response of photosynthesis. Phenotypic plasticity allows for a wide arra...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-013-9816-3
更新日期：2014-02-01 00:00:00
abstract：:The oxidation kinetics under far-red light (FRL) of photosystem I (PSI) high potential donors P700, plastocyanin (PC), and cytochrome f (Cyt f) were investigated in sunflower leaves with the help of a new high-sensitivity photometer at 810 nm. The slopes of the 810 nm signal were measured immediately before and after ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-007-9224-7
更新日期：2007-10-01 00:00:00
abstract：:The xanthophyll cycle represents one of the important photoprotection mechanisms in plant cells. In the present review, we summarize current knowledge about the violaxanthin cycle of vascular plants, green and brown algae, and the diadinoxanthin cycle of the algal classes Bacillariophyceae, Xanthophyceae, Haptophyceae...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-010-9536-x
更新日期：2010-11-01 00:00:00
abstract：:In this paper, we describe a protocol to obtain a site-directed mutants in thepsbA gene ofChlamydomonas reinhardtii, which overcomes several drawbacks of previous protocols, and makes it possible to generate a mutant within a month. Since the large size of the gene, and the presence of four large introns has made mole...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF02187123
更新日期：1994-11-01 00:00:00
abstract：:I provide here a personal perspective on (i) the awards that were given to young investigators at the 2008 and 2009 Gordon Research Conferences on Biochemistry/Biophysics of Photosynthesis; and (ii) the ambiance at these conferences through some photographs, particularly of the 2009 conference. ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-009-9479-2
更新日期：2009-10-01 00:00:00
abstract：:Oxygenic photosynthesis is driven by two multi-subunit membrane protein complexes, Photosystem I and Photosystem II. In plants and green algae, both complexes are composed of two moieties: a reaction center (RC), where light-induced charge translocation occurs, and a peripheral antenna that absorbs light and funnels i...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/B:PRES.0000036881.23512.42
更新日期：2004-01-01 00:00:00
abstract：:Artificial aggregates of bacteriochlorophyllc (BChlc) were formed in an aqueous medium in the presence of a lipid, monogalactosyl diglyceride (MGDG), and the optical properties of those aggregates were studied by absorption and circular dichroism (CD) mainly. Four BChlc homologs, ([E,E]BChlc F, [P,E]BChlc F, [E,M]BChl...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF02184164
更新日期：1994-07-01 00:00:00
abstract：:The cyanobacterial small CAB-like proteins (SCPs) are one-helix proteins with compelling similarity to the first and third transmembrane helix of proteins belonging to the CAB family of light-harvesting complex proteins in plants. The SCP proteins are transiently expressed at high light intensity and other stress cond...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-007-9244-3
更新日期：2008-02-01 00:00:00
abstract：:Several protein-coding genes from land plant chloroplasts have been shown to contain introns. The majority of these introns resemble the fungal mitochondrial group II introns due to considerable nucleotide sequence homology at their 5' and 3' ends and they can readily be folded to form six hairpins characteristic of t...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00039484
更新日期：1988-04-01 00:00:00
abstract：:We have constructed a mutant Synechococcus sp. PCC 7942, termed R2HECAT, in which the entire phycobilisome rod operon has been deleted. In the whole cell absorption spectra of R2HECAT, the peak corresponding to phycocyanin (PC), λmax≈620 nm, could not be detected. However, a single pigment-protein fraction with λmax=6...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00032236
更新日期：1995-07-01 00:00:00
abstract：:DCMU (3-(3,4-dichlorophenyl)-1,1-dimethylurea) at concentrations higher than 10 μM suppresses the second time range delayed fluorescence (DF) of pea chloroplasts, due to inhibition of the oxidizing side of photosystem II (PS II). The inhibition of the reducing side of PS II resulting in the suppression of millisecond ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00054986
更新日期：1988-01-01 00:00:00
abstract：:We present a theoretical study of excitation dynamics in the chlorosome antenna complex of green photosynthetic bacteria based on a recently proposed model for the molecular assembly. Our model for the excitation energy transfer (EET) throughout the antenna combines a stochastic time propagation of the excitonic wave ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-014-9978-7
更新日期：2014-06-01 00:00:00
abstract：:Pulse modulated fluorescence has increasingly been used as an ecological tool to examine changes in the vertical distribution of microphytobenthic cells within the upper layers of estuarine sediments (most often using the minimum fluorescence yield F(o)) as well as to indicate the health of the community (using the ma...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/B:PRES.0000028397.86495.b5
更新日期：2004-01-01 00:00:00
abstract：:Ferredoxin and the flavoprotein, ferredoxin: NADP reductase, have been covalently linked by incubation in the presence of a water soluble carbodiimide. The cross-linking reaction yields an adduct having a 1:1 stoichiometry. The adduct has depressed levels of diaphorase and NADPH oxidase activity and is inactive in red...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00035450
更新日期：1988-09-01 00:00:00
abstract：:In polyploid plants the photosynthetic rate per cell is correlated with the amount of DNA per cell. The photosynthetic rate per unit leaf area is the product of the rate per cell times the number of photosynthetic cells per unit area. Therefore, the photosynthetic rate per unit leaf area will increase if there is a le...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00014744
更新日期：1993-02-01 00:00:00
abstract：:A pigment system containing carotenoids and oxidised reaction centre pigments is present in chromatophores of Rhodospirillum rubrum and this pigment system may cause fluorescence quenching when a still unidentified chromatophore component is in its oxidised state. Besides by its action spectrum, this pigment system is...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00018376
更新日期：1984-03-01 00:00:00
abstract：:Recent magnetic-resonance work on YŻ suggests that this species exhibits considerable motional flexibility in its functional site and that its phenol oxygen is not involved in a well-ordered hydrogen-bond interaction (Tang et al., submitted; Tommos et al., in press). Both of these observations are inconsistent with a ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00020428
更新日期：1995-11-01 00:00:00
abstract：:Significance of molecular crowding in grana thylakoids of higher plants on photosystem II function was studied by 'titrating' the naturally high protein density by fusing unilamellar liposomes of the native lipid mixture with isolated grana membranes (BBY). The incorporation of lipids was monitored by equilibrium dens...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-007-9253-2
更新日期：2008-02-01 00:00:00
abstract：:Chlorophyll (Chl) b serves an essential function in accumulation of light-harvesting complexes (LHCs) in plants. In this article, this role of Chl b is explored by considering the properties of Chls and the ligands with which they interact in the complexes. The overall properties of the Chls, not only their spectral f...
journal_title：Photosynthesis research
pub_type： 杂志文章,评审
doi：10.1007/s11120-007-9181-1
更新日期：2007-11-01 00:00:00
abstract：:A detailed analysis of the periplasmic electron carriers of the photosynthetic bacterium Ectothiorhodospira sp. has been performed. Two low mid-point redox potential electron carriers, cytochrome c' and cytochrome c, are detected. A high potential iron-sulfur protein is the only high mid-point redox potential electron...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1006486307473
更新日期：2000-01-01 00:00:00
abstract：:Energy transfer of the light harvesting complex LHC-II trimer, extracted from spinach, was studied in the Q(y) region at room temperature by femtosecond transient absorption spectroscopy. Configuration interaction exciton method [Linnanto et al. (1999) J Phys Chem B 103: 8739-8750] and 2.72 A structural information re...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-005-9004-1
更新日期：2006-03-01 00:00:00
abstract：:The chloroplast photo-oxidation and the expression of the Cab gene Lhcb1, encoding the Lhcb1 light-harvesting chlorophyll a/b-protein of PS II, have been studied in leaf cells of maize treated with the two bleaching herbicides norflurazon and amitrole and of the two carotenoid-free mutants vp9 and vp2 grown under high...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1023/A:1006477215572
更新日期：2000-01-01 00:00:00
abstract：:The photosynthetic green sulfur bacterium Chlorobaculum (Cba.) tepidum produces bacteriochlorophyll (BChl) c pigments bearing a chiral 1-hydroxyethyl group at the 3-position, which self-aggregate to construct main light-harvesting antenna complexes, chlorosomes. The secondary alcoholic hydroxy group is requisite for c...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-016-0220-7
更新日期：2016-12-01 00:00:00
abstract：:A simple approach to determine CO2/O2 specificity factor (τ) of ribulose 1,5-bisphosphate carboxylase/oxygenase is described. The assay measures the amount of CO2 fixation at varying [CO2]/[O2] ratios after complete consumption of ribulose 1,5-bisphosphate (RuBP). Carbon dioxide fixation catalyzed by the carboxylase w...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00029463
更新日期：1995-01-01 00:00:00
abstract：:Precise control of the proton concentration gradient across thylakoid membranes (ΔpH) is essential for photosynthesis and its regulation because the gradient contributes to the generation of the proton motive force used for ATP synthesis and also for the fast and reversible induction of non-photochemical quenching (NP...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-018-0575-z
更新日期：2019-03-01 00:00:00
abstract：:Temperature response of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) catalytic properties directly determines the CO2 assimilation capacity of photosynthetic organisms as well as their survival in environments with different thermal conditions. Despite unquestionable importance of Rubisco, the comprehensi...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-014-0067-8
更新日期：2015-02-01 00:00:00
abstract：:Data on cadmium accumulation in chloroplasts of terrestrial plants are scarce and contradictory. We introduced CdSO4 in hydroponic media to the final concentrations 80 and 250 μM and studied the accumulation of Cd in chloroplasts of Hordeum vulgare and Zea mays. Barley accumulated more Cd in the chloroplasts as compar...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-014-0047-z
更新日期：2015-08-01 00:00:00
abstract：:Lipids are important components of transmembrane protein complexes. In order to study the roles of lipids in photosystem II (PSII), we treated the PSII core dimer complex from a thermophilic cyanobacterium Thermosynechococcus vulcanus with phospholipase A(2) (PLA(2)) and lipase, and examined their effects on PSII stru...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/s11120-008-9335-9
更新日期：2008-10-01 00:00:00
abstract：:A rapid procedure has been developed for the isolation of the photosystem two reaction centre complex (PS II RC) from a double mutant of Chlamydomonas reinhardtii, F54-14, which lacks the Photosystem one complex and the chloroplast ATPase. Thylakoid membranes are solubilised with 1.5% (w/v) Triton X-100 and the PS II ...
journal_title：Photosynthesis research
pub_type： 杂志文章
doi：10.1007/BF00042974
更新日期：1995-02-01 00:00:00
