Chemical oxidation of the FMO antenna protein from Chlorobaculum tepidum.

Abstract:

:Effect of chemical oxidation by ferricyanide on bacteriochlorophyll a (BChl a) in the Fenna-Matthews-Olson protein (FMO) was studied using absorbance and fluorescence spectroscopy at ambient and cryogenic temperatures. Partially selective oxidation of pigments bound to the antenna complex was achieved and the probable absorption wavelength corresponding to the recently discovered bacteriochlorophyll No. 8 of 806 nm was obtained by comparative analysis of the effect of chemical oxidation and the effect of different isolation procedures. Formation of a stable product identified as a chlorophyll a derivative occurred upon chemical oxidation. This new pigment remained bound within the pigment-protein complex, and exhibited an efficient energy transfer to BChl a. Furthermore, complex effects of the pigment oxidation upon the fluorescence yield of the FMO protein were observed. Utility of this approach based on chemical modifications for the investigation of the native regulatory mechanisms involved in the energy transfer in the FMO protein is discussed.

journal_name

Photosynth Res

journal_title

Photosynthesis research

authors

Bina D,Blankenship RE

doi

10.1007/s11120-013-9878-2

subject

Has Abstract

pub_date

2013-09-01 00:00:00

pages

11-9

issue

1

eissn

0166-8595

issn

1573-5079

journal_volume

116

pub_type

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