Abstract:
:Effect of chemical oxidation by ferricyanide on bacteriochlorophyll a (BChl a) in the Fenna-Matthews-Olson protein (FMO) was studied using absorbance and fluorescence spectroscopy at ambient and cryogenic temperatures. Partially selective oxidation of pigments bound to the antenna complex was achieved and the probable absorption wavelength corresponding to the recently discovered bacteriochlorophyll No. 8 of 806 nm was obtained by comparative analysis of the effect of chemical oxidation and the effect of different isolation procedures. Formation of a stable product identified as a chlorophyll a derivative occurred upon chemical oxidation. This new pigment remained bound within the pigment-protein complex, and exhibited an efficient energy transfer to BChl a. Furthermore, complex effects of the pigment oxidation upon the fluorescence yield of the FMO protein were observed. Utility of this approach based on chemical modifications for the investigation of the native regulatory mechanisms involved in the energy transfer in the FMO protein is discussed.
journal_name
Photosynth Resjournal_title
Photosynthesis researchauthors
Bina D,Blankenship REdoi
10.1007/s11120-013-9878-2subject
Has Abstractpub_date
2013-09-01 00:00:00pages
11-9issue
1eissn
0166-8595issn
1573-5079journal_volume
116pub_type
杂志文章abstract::We have used antibodies generated against synthetic peptides to determine the topology of the 43 kD chlorophyll a binding protein (CP 43) of Photosystem II. Based on the pattern of proteolytic fragments detected (on western blots) by peptide specific antibodies, a six transmembrane span topological model, with the ami...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00019041
更新日期:1994-04-01 00:00:00
abstract::The Fabaceae tree Butea monosperma (TAUB.; syn. Erythrina monosperma (LAM.)) is widely distributed in Central and West-India. We studied it at three sites, i.e. at two locations with contrasting exposure (NE and SW, respectively) in a small mountain range with poor soil on highly drained rocky slopes and at a third lo...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-012-9770-5
更新日期:2012-09-01 00:00:00
abstract::Six chlorophyll-protein complexes are isolated from thylakoid membranes of Bryopsis corticulans by dodecyl-beta-D: -maltoside polyacrylamide gel electrophoresis. Unlike that of higher plants, the 77 K fluorescence emission spectrum of the CP1 band, the PSI core complexes of B. corticulans, presents two peaks, one at 6...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-007-9286-6
更新日期:2008-04-01 00:00:00
abstract::Bill Arnold's concept of photosynthesis as a solid state, as opposed to solution, phenomenon led him to an amazing series of fundamental discoveries. ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00040990
更新日期:1996-05-01 00:00:00
abstract::Various techniques of electron microscopy (EM) such as ultrathin sectioning, freeze-fracturing, freeze-etching, negative staining and (cryo-)electron crystallography of two-dimensional crystals have been employed, since now, to obtain much of the structural information of the Photosystem II (PS II) pigment-protein com...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1024927631196
更新日期:2003-01-01 00:00:00
abstract::The author gives an autobiographical sketch of his path to chlorophyll research, and describes some results. The discussion is largely focused on long wavelength forms of chlorophyll and how they might be generated by self-assembly. Dimers or oligomers, (Chl)n, result from coordination interactions between the central...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00033128
更新日期:1990-12-01 00:00:00
abstract::A protocol for the isolation of functional thylakoids from Arabidopsis thaliana leaves was developed. The critical factor in obtaining active, coupled and stable preparation is the inclusion of EDTA and EGTA in the grinding buffer. Preparations were characterized with respect to the whole or partial electron transport...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1011818021875
更新日期:2001-01-01 00:00:00
abstract::The linear, four-step oxidation of water to molecular oxygen by photosystem II requires cooperation between redox reactions driven by light and a set of redox reactions involving the S-states within the oxygen-evolving complex. The oxygenevolving complex is a highly ordered structure in which a number of polypeptides ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00037001
更新日期:1985-01-01 00:00:00
abstract::The electronic structure of a Mn(II) ion bound to highly oxidizing reaction centers of Rhodobacter sphaeroides was studied in a mutant modified to possess a metal binding site at a location comparable to the Mn4Ca cluster of photosystem II. The Mn-binding site of the previously described mutant, M2, contains three car...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-013-9887-1
更新日期:2014-05-01 00:00:00
abstract::Optically detected magnetic resonance of chlorosome-containing membranes from the green filamentous bacterium Chloroflexus aurantiacus has been performed both by fluorescence and absorption detection. Triplet states localized in the chlorosomes and in the B808-866 complex have been characterized. After chemical reduct...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1014947412940
更新日期:2002-01-01 00:00:00
abstract::Modulation of the efficiency with which leaves convert absorbed light to photochemical energy [intrinsic efficiency of open photosystem II (PSII) centers, as the ratio of variable to maximal chlorophyll fluorescence] as well as leaf xanthophyll composition (interconversions of the xanthophyll cycle pigments violaxanth...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-012-9761-6
更新日期:2012-09-01 00:00:00
abstract::Polyamines (PAs) are small metabolites that are produced and oxidized in chloroplasts with an obscure mode of action. Recently, we showed that qE is stimulated by PAs in higher plants (Nicotiana tabacum) and in genetically modified plants with elevated thylakoid-associated PAs (Ioannidis and Kotzabasis Biochim Biophys...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-010-9617-x
更新日期:2011-02-01 00:00:00
abstract::This concise review describes the current status of research into how carotenoids function in bacterial photosynthesis. This is illustrated by reference to very recent studies on both the photoprotective and antenna functions of carotenoids. The major remaining open questions on the detailed molecular mechanisms invol...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1014715114520
更新日期:2001-01-01 00:00:00
abstract::A protein was isolated from membranes of the green sulfur bacterium Chlorobium tepidum. This protein was characterized by gel electrophoresis, gel filtration, analytical ultracentrifugation and amino acid sequencing. The molecular weight of the purified protein was shown to be 26 kDa by SDS-PAGE. HPLC gelfiltration, S...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/B:PRES.0000015383.58680.56
更新日期:2004-02-01 00:00:00
abstract::A new binding site for anions which inhibit the water oxidizing complex (WOC) of Photosystem II in spinach has been identified. Anions which bind to this site inhibit the flash-induced S2/S0 catalase reaction (2H2O2→2H2O+O2) of the WOC by displacing hydrogen peroxide. Using a mass spectrometer and gas permeable membra...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00046771
更新日期:1993-01-01 00:00:00
abstract::Two green algal species, Chlamydomonas reinhardtii and Scenedesmus obliquus, exhibited a relative maximum during the decay of luminescence, when adapted to low CO2 conditions that was not observed in high CO2 adapted cells.From the kinetics of transient changes in the level of dark fluorescence, after illumination and...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00034857
更新日期:1990-03-01 00:00:00
abstract::Photosynthesis was well-established on the earth at least 3.5 thousand million years ago, and it is widely believed that these ancient organisms had similar metabolic capabilities to modern cyanobacteria. This requires that development of two photosystems and the oxygen evolution capability occurred very early in the...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:
更新日期:1992-01-01 00:00:00
abstract::Plant terpenoids are among the most diverse group of naturally-occurring organic compounds known, and several are used in contemporary consumer products. Terpene synthase enzymes catalyze complex rearrangements of carbon skeleton precursors to yield thousands of unique chemical structures that range in size from the s...
journal_title:Photosynthesis research
pub_type: 杂志文章,评审
doi:10.1007/s11120-014-9979-6
更新日期:2015-03-01 00:00:00
abstract::The thylakoid membrane protein PsbS is critical for quenching excessive excitation energy in mechanisms that involve the light-harvesting complexes of photosystem II. Liposomes of thylakoid lipids have been shown to be a very good platform to study photosynthetic membrane proteins and their interactions. In this study...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-015-0176-z
更新日期:2016-01-01 00:00:00
abstract::The dependence on temperature of tryptophan fluorescence lifetime in trimeric photosystem I (PSI) complexes from cyanobacteria Synechocystis sp. PCC 6803 during the heating of pre-frozen to - 180 °C in the dark or in the light-activated preparations has been studied. Fluorescence lifetime in samples frozen in the ligh...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-018-0595-8
更新日期:2019-03-01 00:00:00
abstract::Using 77 K chlorophyll a (Chl a) fluorescence spectra in vivo, the development was studied of Photosystems II (PS II) and I (PS I) during greening of barley under intermittent light followed by continuous light at low (LI, 50 mumol m(-2) s(-1)) and high (HI, 1000 mumol m(-2) s(-1)) irradiances. The greening at HI inte...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1023/A:1020943107318
更新日期:2002-01-01 00:00:00
abstract::NADP:ferredoxin oxidoreductase (EC. 1.6.7.1.) isolated from a thermophilic blue-green alga, Synechococcus sp., was stable at temperatures up to 65°C. The diaphorase and cytochrome c reductase activities of the enzyme were low at 25°C but increased with elevated temperature to reach a maximum at about 60°C. The pH-prof...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00020595
更新日期:1980-09-01 00:00:00
abstract::A comparative study of photosystem II complexes isolated from tobacco (Nicotiana tabacum L. cv. John William's Broadleaf) which contains normal stacked thylakoid membranes, and from two chlorophyll deficient tobacco mutants (Su/su and Su/su var. Aurea) which have low stacked grana or essentially unstacked thylakoids w...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00032264
更新日期:1987-01-01 00:00:00
abstract::Productivity of most macroalgae is not currently considered limited by dissolved inorganic carbon (DIC), as the majority of species have CO2-concentrating mechanisms (CCM) allowing the active uptake of DIC. The alternative, diffusive uptake of CO2 (non-CCM), is considered rare (0-9% of all macroalgal cover in a given ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-015-0114-0
更新日期:2015-05-01 00:00:00
abstract::Cross-reconstitution of the extrinsic proteins and Photosystem II (PS II) from a green alga, Chlamydomonas reinhardtii, and a higher plant,Spinacia oleracea, was performed to clarify the differences of binding properties of the extrinsic proteins between these two species of organisms. (1) Chlamydomonas PsbP and PsbQ ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-004-7760-y
更新日期:2005-06-01 00:00:00
abstract::Dry weight and Relative Growth Rate of Lemna gibba were significantly increased by CO2 enrichment up to 6000 μl CO2 l(-1). This high CO2 optimum for growth is probably due to the presence of nonfunctional stomata. The response to high CO2 was less or absent following four days growth in 2% O2. The Leaf Area Ratio decr...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00029048
更新日期:1985-03-01 00:00:00
abstract::In this communication, evidence is presented from the kinetics of QA (-) decay (where QA is the first plastoquinone electron acceptor of photosystem II) and oxygen evolution for the requirement of bicarbonate in the electron transport in a cyanobacteriumSynechocystis (Pasteur Culture Collection 6803). A large slowing ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00046879
更新日期:1988-10-01 00:00:00
abstract::Cyanobacteria, algae, and plants produce dioxygen from water. Driven and clocked by light quanta, the catalytic Mn(4)Ca Tyrosine centre accumulates four oxidizing equivalents before it abstracts four electrons from water and liberates dioxygen and protons. Intermediates of this reaction cascade are short-lived (<100 m...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/s11120-008-9321-2
更新日期:2008-10-01 00:00:00
abstract::Chloroplasts of a chlorophyll (Chl) b-less barley mutant were solubilized with digitonin and fractionated by polyacrylamide gel electrophoresis with sodium deoxycholate in the running buffer. By this procedure, in contrast to using sodium dodecylsulfate (SDS) for solubilization, a Chl a-protein analogous to the major ...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00018272
更新日期:1987-01-01 00:00:00
abstract::This review describes the progress in our understanding of the structure of the Mn complex in Photosystem II over the last two decades. Emphasis is on the research from our laboratory, especially the results from X-ray absorption spectroscopy, low temperature electron paramagnetic resonance and electron spin echo enve...
journal_title:Photosynthesis research
pub_type: 杂志文章
doi:10.1007/BF00046751
更新日期:1993-01-01 00:00:00